WO2009049223A3 - Methods and compositions for the site-selective incorporation of fluorinated amino acids into polypeptides - Google Patents

Methods and compositions for the site-selective incorporation of fluorinated amino acids into polypeptides Download PDF

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Publication number
WO2009049223A3
WO2009049223A3 PCT/US2008/079603 US2008079603W WO2009049223A3 WO 2009049223 A3 WO2009049223 A3 WO 2009049223A3 US 2008079603 W US2008079603 W US 2008079603W WO 2009049223 A3 WO2009049223 A3 WO 2009049223A3
Authority
WO
WIPO (PCT)
Prior art keywords
compositions
polypeptides
site
methods
amino acids
Prior art date
Application number
PCT/US2008/079603
Other languages
French (fr)
Other versions
WO2009049223A2 (en
Inventor
Susan E Cellitti
David H Jones
Youngha Ryu
Peter G. Schultz
Bernhard H. Geierstanger
Original Assignee
Irm Llc
The Scripps Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Irm Llc, The Scripps Research Institute filed Critical Irm Llc
Publication of WO2009049223A2 publication Critical patent/WO2009049223A2/en
Publication of WO2009049223A3 publication Critical patent/WO2009049223A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B59/00—Introduction of isotopes of elements into organic compounds ; Labelled organic compounds per se
    • C07B59/008—Peptides; Proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/006—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length of peptides containing derivatised side chain amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/13—Labelling of peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)

Abstract

Compositions including orthogonal aminoacyl-tRNA synthetases (O-RS) that preferentially aminoacylate an orthogonal tRNA (O-tRNA) with trifluoromethoxyphenylalanine are provided. Nucleic acids encoding these aminoacyl-tRNA synthetases are also provided.
PCT/US2008/079603 2007-10-10 2008-10-10 Methods and compositions for the site-selective incorporation of fluorinated amino acids into polypeptides WO2009049223A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US97903607P 2007-10-10 2007-10-10
US60/979,036 2007-10-10
US3148008P 2008-02-26 2008-02-26
US61/031,480 2008-02-26

Publications (2)

Publication Number Publication Date
WO2009049223A2 WO2009049223A2 (en) 2009-04-16
WO2009049223A3 true WO2009049223A3 (en) 2009-11-05

Family

ID=40430072

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/079603 WO2009049223A2 (en) 2007-10-10 2008-10-10 Methods and compositions for the site-selective incorporation of fluorinated amino acids into polypeptides

Country Status (1)

Country Link
WO (1) WO2009049223A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10501734B2 (en) 2014-02-06 2019-12-10 Yale University Compositions and methods of use thereof for making polypeptides with many instances of nonstandard amino acids
US11408007B2 (en) 2014-09-26 2022-08-09 Yale University Compositions and methods for biocontainment of microorganisms
US11149280B2 (en) 2019-10-29 2021-10-19 Yale University Engineering organisms resistant to viruses and horizontally transferred genetic elements
WO2023196596A2 (en) * 2022-04-08 2023-10-12 Absci Corporation Trna synthetases and methods of use thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006034410A2 (en) * 2004-09-21 2006-03-30 The Scripps Resarch Institute Adding photoregulated amino acids to the genetic code
WO2006036779A2 (en) * 2004-09-22 2006-04-06 The Scripps Research Institute Site-specific labeling of proteins for nmr studies
WO2006110182A2 (en) * 2004-10-27 2006-10-19 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids
WO2007092475A2 (en) * 2006-02-06 2007-08-16 Franklin And Marshall College Site-specific incorporation of fluorinated amino acids into proteins

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006034410A2 (en) * 2004-09-21 2006-03-30 The Scripps Resarch Institute Adding photoregulated amino acids to the genetic code
WO2006036779A2 (en) * 2004-09-22 2006-04-06 The Scripps Research Institute Site-specific labeling of proteins for nmr studies
WO2006110182A2 (en) * 2004-10-27 2006-10-19 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids
WO2007092475A2 (en) * 2006-02-06 2007-08-16 Franklin And Marshall College Site-specific incorporation of fluorinated amino acids into proteins

Also Published As

Publication number Publication date
WO2009049223A2 (en) 2009-04-16

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