WO2009141163A3 - Bioreaktor und verfahren zum kultivieren von zellen und geweben - Google Patents

Bioreaktor und verfahren zum kultivieren von zellen und geweben Download PDF

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Publication number
WO2009141163A3
WO2009141163A3 PCT/EP2009/003682 EP2009003682W WO2009141163A3 WO 2009141163 A3 WO2009141163 A3 WO 2009141163A3 EP 2009003682 W EP2009003682 W EP 2009003682W WO 2009141163 A3 WO2009141163 A3 WO 2009141163A3
Authority
WO
WIPO (PCT)
Prior art keywords
bioreactor
tissues
cultivating cells
tissue culture
relates
Prior art date
Application number
PCT/EP2009/003682
Other languages
English (en)
French (fr)
Other versions
WO2009141163A2 (de
Inventor
Günther KNEBEL
Sven MÜHLFRIEDEL
Original Assignee
Greiner Bio - One Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Greiner Bio - One Gmbh filed Critical Greiner Bio - One Gmbh
Priority to EP09749653A priority Critical patent/EP2283109A2/de
Priority to US12/736,866 priority patent/US20110111504A1/en
Publication of WO2009141163A2 publication Critical patent/WO2009141163A2/de
Publication of WO2009141163A3 publication Critical patent/WO2009141163A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/40—Manifolds; Distribution pieces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/12—Pulsatile flow
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/14—Pressurized fluid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/16—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature by recirculation of culture medium at controlled temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH

Abstract

Die Erfindung betrifft einen Bioreaktor (1) für Zell- und Gewebekultur zur mechanischen Stimulation und/oder Perfusion von Gewebe- bzw. Zellkulturen umfassend ein Oberteil (3) und ein damit verbundenes Unterteil (4) mit einer Gewebekulturkammer (8), wobei in der Gewebekulturkammer (8) zumindest eine Verteilerplatte (12) angeordnet ist, sowie ein Verfahren zur Kultivierung von Zellen und Geweben im Bioreaktor (1).
PCT/EP2009/003682 2008-05-23 2009-05-25 Bioreaktor und verfahren zum kultivieren von zellen und geweben WO2009141163A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP09749653A EP2283109A2 (de) 2008-05-23 2009-05-25 Bioreaktor und verfahren zum kultivieren von zellen und geweben
US12/736,866 US20110111504A1 (en) 2008-05-23 2009-05-25 Bioreactor and method for cultivating cells and tissues

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT0082308A AT506826B1 (de) 2008-05-23 2008-05-23 Bioreaktor und verfahren zum kultivieren von zellen und geweben
ATA823/2008 2008-05-23

Publications (2)

Publication Number Publication Date
WO2009141163A2 WO2009141163A2 (de) 2009-11-26
WO2009141163A3 true WO2009141163A3 (de) 2012-01-12

Family

ID=41340603

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2009/003682 WO2009141163A2 (de) 2008-05-23 2009-05-25 Bioreaktor und verfahren zum kultivieren von zellen und geweben

Country Status (4)

Country Link
US (1) US20110111504A1 (de)
EP (1) EP2283109A2 (de)
AT (1) AT506826B1 (de)
WO (1) WO2009141163A2 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20110091078A (ko) * 2010-02-05 2011-08-11 전민용 미생물의 오염을 방지하는 줄기세포 배양용기
DE102010039229A1 (de) * 2010-08-11 2012-02-16 Universität Potsdam Perfusionsvorrichtung
KR20130044912A (ko) * 2011-10-25 2013-05-03 삼성전기주식회사 배양 장치 및 배양액 교체 방법
WO2014111518A1 (en) * 2013-01-17 2014-07-24 Medibiome Ab Tissue bioreactor
CN109689853B (zh) * 2016-08-27 2022-08-23 三维生物科技有限公司 生物反应器
US11680241B2 (en) 2018-02-08 2023-06-20 University Of Florida Research Foundation, Inc. Perfusion enabled bioreactors
DE102018004890B4 (de) * 2018-06-19 2023-03-16 Sartorius Stedim Biotech Gmbh Filtersystem für biopharmazeutische Prozesse, Verteilerplatte für ein Filtersystem und Verfahren zum Herstellen eines Filtersystems
US20220195368A1 (en) * 2020-12-21 2022-06-23 Upside Foods, Inc. Fluid conduit with radial expansion of fluid flow

Citations (8)

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US6060306A (en) * 1995-06-07 2000-05-09 Advanced Tissue Sciences, Inc. Apparatus and method for sterilizing, seeding, culturing, storing, shipping and testing replacement cartilage tissue constructs
WO2000055299A1 (de) * 1999-03-15 2000-09-21 Wolfgang Fege Vorrichtung zum züchten von menschlichem oder tierischem gewebe
WO2001009282A2 (de) * 1999-07-29 2001-02-08 Augustinus Bader Vorrichtung zum züchten und/oder behandeln von zellen
DE19964113A1 (de) * 1999-12-31 2001-07-05 Joerg C Gerlach Vorrichtung und Verfahren zur Züchtung und zur Nutzung von Hautzellen
US20050095711A1 (en) * 2003-11-01 2005-05-05 More Robert B. Bioreactor for growing engineered tissue
WO2005040332A2 (de) * 2003-10-21 2005-05-06 Universität Leipzig Verfahren und bioreaktor zum kultivieren und stimulieren von dreidimensionalen, vitalen und mechanisch widerstandsfähigen zelltransplantaten
WO2005087912A2 (de) * 2004-03-10 2005-09-22 Fachhochschule Giessen-Friedberg Erfindung betreffend bioreaktoren und bioreaktorsysteme
US20060110822A1 (en) * 2004-09-16 2006-05-25 Robbins Neil F Perfusion bioreactors for culturing cells

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US4806246A (en) * 1986-04-11 1989-02-21 Applied Membrane Technology, Inc. Pore size control using plasma polymerization techniques
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DE4306661C2 (de) * 1993-03-03 1995-04-20 Michael Dipl Biol Sittinger Verfahren zum Herstellen eines Implantates aus Zellkulturen
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Publication number Priority date Publication date Assignee Title
US6060306A (en) * 1995-06-07 2000-05-09 Advanced Tissue Sciences, Inc. Apparatus and method for sterilizing, seeding, culturing, storing, shipping and testing replacement cartilage tissue constructs
WO2000055299A1 (de) * 1999-03-15 2000-09-21 Wolfgang Fege Vorrichtung zum züchten von menschlichem oder tierischem gewebe
WO2001009282A2 (de) * 1999-07-29 2001-02-08 Augustinus Bader Vorrichtung zum züchten und/oder behandeln von zellen
DE19964113A1 (de) * 1999-12-31 2001-07-05 Joerg C Gerlach Vorrichtung und Verfahren zur Züchtung und zur Nutzung von Hautzellen
WO2005040332A2 (de) * 2003-10-21 2005-05-06 Universität Leipzig Verfahren und bioreaktor zum kultivieren und stimulieren von dreidimensionalen, vitalen und mechanisch widerstandsfähigen zelltransplantaten
US20050095711A1 (en) * 2003-11-01 2005-05-05 More Robert B. Bioreactor for growing engineered tissue
WO2005087912A2 (de) * 2004-03-10 2005-09-22 Fachhochschule Giessen-Friedberg Erfindung betreffend bioreaktoren und bioreaktorsysteme
US20060110822A1 (en) * 2004-09-16 2006-05-25 Robbins Neil F Perfusion bioreactors for culturing cells

Also Published As

Publication number Publication date
AT506826A1 (de) 2009-12-15
US20110111504A1 (en) 2011-05-12
WO2009141163A2 (de) 2009-11-26
EP2283109A2 (de) 2011-02-16
AT506826B1 (de) 2010-03-15

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