WO2009139665A1 - Лекарственное средство, обладающее противопротозойной активностью в отношении тriсhоmоnаs vаginаlis в модельной системе in vitrо - Google Patents
Лекарственное средство, обладающее противопротозойной активностью в отношении тriсhоmоnаs vаginаlis в модельной системе in vitrо Download PDFInfo
- Publication number
- WO2009139665A1 WO2009139665A1 PCT/RU2009/000215 RU2009000215W WO2009139665A1 WO 2009139665 A1 WO2009139665 A1 WO 2009139665A1 RU 2009000215 W RU2009000215 W RU 2009000215W WO 2009139665 A1 WO2009139665 A1 WO 2009139665A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- vagipalis
- concentration
- trichomonas
- drug
- strains
- Prior art date
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/13—Coniferophyta (gymnosperms)
- A61K36/15—Pinaceae (Pine family), e.g. pine or cedar
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
- A61K31/05—Phenols
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
Definitions
- the invention relates to medicine and can be used to treat various forms of trichomonas infection, both in acute diseases of the urogenital tract and in chronic and latent forms of infection.
- Trichomoniasis ranks first in terms of prevalence among diseases of the genitourinary tract and holds first place among sexually transmitted diseases. This infection has great medical and socio-economic importance due to the high infection of people, often a chronic course with relapses, damage to various organs and systems.
- Genitourinary trichomoniasis is transmitted through sexual contact from a sick person or carrier, sometimes there is also a non-sexual route of infection: children from sick mothers during childbirth, a contact transmission path through non-disposable gloves during examination, lining diapers, ships, night pots, toilet seats, general items personal hygiene and others (Isakov BA, Zakharkiv Yu.F., Ermolenko D.K. et al. Diagnostics and treatment of urogenital trichomoniasis, Recommendations for doctors, St. Moscow, Veliky Novgorod, 2006, 49c).
- trichomonasus The presence of low-symptom forms and trichomonasus is important in the epidemic process, which contributes to the epidemiological spread of the pathogen. Without treatment, these protozoa do not leave the host and are capable of causing all kinds of complications. So, trichomoniasis was diagnosed in 70-80 year old men with the last sexual relationship thirty years ago.
- Known drugs used to treat trichomonal infections which give a slight decrease in symptoms. These include acrychin, aminarson, dichlorodiphenyl trichloroethane, trichomycin, etc. However, these drugs are ineffective.
- phytotherapy methods extracts and tinctures obtained from onions, garlic, radish, horseradish, needles of spruce, pine, and juniper.
- metronidazole synthesized in 1959, is widely used for the treatment of urogenital trichomoniasis.
- complex treatment methods are widely used, which is expressed in the combined use of oral and intravaginal agents - Klion-D, ginalgin, terzhinan, metrogil, patent RU 2320319 “Vaginal cytoprositropy” )), 2006.11.07).
- the position that chronic urogenital trichomoniasis proceeding as a mixed invasion is substantiated is an indicator of the high probability of detecting drug-resistant strains of T. vagipalis.
- New drugs are needed to create shortened as well as integrated treatment methods for urogenital trichomoniasis based on studying pharmacokinetics and determining the sensitivity of different strains of T. vagipalis to the same dose of the drug used.
- Urogenital trichomonads are a depot for the survival of gonococci, fungi, chlamydia, mycoplasmas, viruses (Thomasop JL Trichopas vagipalis / JX. Thomasop, SM Gelbert // Obstr Ap-Gol36-5. for protozoan enzymes cannot always destroy the microorganisms phagocytosed by them. Subsequently, these pathogens can support the inflammatory process for a long time. Trichomonas is known to interfere with the detection of chlamydia in cell cultures. Inflammation of the mycoplasma and chlamydial etiology clinically occurs with relapse, often with damage to the accessory genital glands.
- nitroimidazoles are used to treat urogenital trichomoniasis along with metronidazole: tinidazole, ornidazole, secnidazole, nimorazole, carnidazole.
- the closest reference drug was the drug metronidazole, which, as a rule, is always included as a treatment for trichomoniasis in individual or complex therapy. Disclosure of invention
- the problem to which the invention is directed is to create a new herbal medicine for the treatment of various forms of Trichomonas infection with minimal side effect.
- a medicinal product of plant origin was found to have antiprotozoal activity against Trichomes vagipalis in the model vitro system, which is a coniferous chlorophyll-carotene paste (CCP).
- CCP coniferous chlorophyll-carotene paste
- HKHP is characterized by the fact that at a concentration of at least 100 mg / ml it exhibits trichomonaddocytic and trichomonadostatic properties, and at a concentration of 300 mg / ml it inhibits the growth of Trichomopas vagipalis.
- HKHP on the parasitic protozoan Trichopathy vagipalis in the model vitro system and the effect of this drug on various forms of Trichoptosis vagipalis isolated from sick people at the final concentrations of 100, 200, 300 and 500 mg / ml was studied.
- a herbal remedy is an olive-colored paste with a moisture content of 35-40%, has a smell of needles.
- Pasta is part of a drug produced under the name
- Bioeffective-A in which HKHP (substance) is enclosed in capsules.
- ChCCP includes chlorophyll derivatives, carotenoids, vitamins A, E, K, phytosterols, polyprenols, squalene, sodium salts of fatty and resin acids, natural antibiotics - phytoncides, essential oils and other terpenoids (labdanic alcohols, aldehydes and acids.
- the causative agent of the infection is the parasitic protozoan Trichopaths vagipalis, isolated from patients, cultured on nutrient media in vitro.
- Nutrient medium with a volume of 4.5 ml was placed in sterile tubes and poured with a layer of sterile vaseline oil with a thickness of 5 mm to create anaerobic conditions for the cultivation of Trichomonas.
- Inoculation was carried out with a sterile Pasteur pipette by placing 0.5 - 1.0 ml of the test material at the bottom of the tubes. Samples were incubated at 37 ° C. Accounting for growth was made after 48 and 96 hours after sowing.
- Trichomonas give bottom growth in the form of a dense whitish precipitate, from which material for microscopic examination is taken with a Pasteur pipette.
- a drop of a warm isotonic sodium chloride solution or Ringer-Locke solution was applied to a glass slide, with which the test sample was mixed.
- the suspension was covered with a coverslip and microscopic with an increase in the x40 objective and xl5 eyepiece.
- a MIKMED-5 brand microscope was used.
- the number of protozoan cells was counted in a Goryaev’s chamber with recalculation per 1 ml in accordance with the instructions for the chamber.
- the research results include several stages. The first stage is the isolation and selection of strains of T. vagipalis. A total of 150 people with various inflammatory diseases of the urogenital tract were examined. Information about patients is presented in table 1.
- strains were isolated to study the antiprotozoal activity of CCPP on the parasitic protozoan Trichomatosis vagipalis in the model vitro system.
- Trichomonas When studying the selected strain, special attention was paid to the size and shape of Trichomonas, the nature of their movement, the internal contents of the cells. In typical cases, trichomonads were found in the form of pear-shaped, less often oval-shaped, motifs with an average size of 13 to 17 microns. The nature of their movements is jerky. Sometimes the movement of free flagella under a microscope was noticeable. The nuclei of Trichomonas in the study of unpainted preparation are poorly distinguishable. The Trichomonas cytoplasm was usually granular, vacuolated.
- the sensitivity of Trichomopas vagipalis to the drug metronidazole in vitro was determined using immobilization of Trichomonas as a criterion.
- tested strains of T. vagipalis should contain at least 90% of motile cells.
- the sensitivity of T. vagipalis strains to metronidazole was determined using the method of serial dilutions of the drug in a nutrient medium.
- the medium is filled with 4.0 ml in sterile tubes, then 0.5 ml of a solution containing different concentrations of metronidazole was added to them: from 0.25 ⁇ g / ml to 1000 ⁇ g / ml (1 mg / ml).
- the sensitivity of trichomonads to metronidazole was assessed by determining the minimum inhibitory concentration (MIC), which causes the immobilization of all T. vagipalis cells.
- the drug-resistant strains included strains in which immobilization is detected at concentrations of metronidazole in excess of 15 ⁇ g / ml.
- the CBT culture method was used.
- HKHP was introduced into the medium in the form of an aqueous solution so that the final concentration corresponded to 100 mg / ml, 200 mg / ml, 300 mg / ml and 500 mg / ml.
- the control sample contained only a suspension of Trichomonas culture in nutrient medium.
- Trichomonas were absent in N ° N ° 1-b and N ° 8.
- the number of pathogen cells in strains ⁇ ⁇ o was 7.9, and 10 was significantly less than in the control.
- a concentration of 300 mg / ml CXKP test drug destroyed the Trichomonas cells of the ⁇ 1 - 9 strains. In the sample containing strain N ° 10, single Trichomes vagipalis cells were found. The introduction into the culture medium of the drug HKHP in a final concentration
- the results obtained showed the presence of anti-trichomonas activity in the HHKP preparation at a concentration of 200 mg / ml.
- Shape dependent Trichomonas vagipalis the presence of previous courses of treatment, the relationship to the action of metronidazole has not been identified.
- the antibacterial effect of CCPI on the concomitant flora was revealed in patients with dysbacterioses of the urogenital tract that arose after previous courses of antibiotic therapy.
- the effective concentration for the four observed samples was at least 300 mg / ml.
- the drug HKHP has antiprotozoal activity against all 10 tested strains of Trichopoda vagipalis isolated from patients with acute and chronic diseases of the urogenital tract;
- the drug exhibits a trichomonadocidal or trichomonadostatic effect.
- the HKHP preparation in 30% of cases exerted a trichomonadocidal effect, and in 70% of them it had a trichomonadostatic effect.
- the drug inhibited the growth of Trichomopas vagipalis;
- the drug KHKP has a destructive effect on both actively mobile and flagellate forms of Trichomonas; 4.
- the antibacterial effect of CCPI on the concomitant flora in patients with dysbiosis that arose after previous courses of antibiotic therapy was established, which suggests the effectiveness of the drug in the treatment of concomitant trichomoniasis diseases, such as dysbiosis for the correction of microbiocenosis of the urogenital tract.
- Example 1 HKHP - 10.0 g bidistilled water - the rest is up to 100g.
- Double-distilled water - the rest is up to 100g.
- Example 3 HHKP - 50.0 g
- Double-distilled water - the rest is up to 100g.
- emulsions (examples 1-3), the calculated amount of CCPP is loaded into the apparatus with a mixing device, the heating is turned on and the paste is heated with stirring to a temperature of +50 0 C and then the calculated amount of bidistilled water is supplied. Upon reaching a homogeneous mass, the heating is turned off, the resulting emulsion is poured into glass shapes.
- HKHP with antibacterial properties in antiprotozoal therapy will accelerate the treatment of patients, not only to achieve growth inhibition
- Trichopaths vagipalis but also suppressing the growth of concomitant pathogenic microflora in urogenital infections, in particular with dysbiosis.
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- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Epidemiology (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
- Alternative & Traditional Medicine (AREA)
- Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Description
Claims
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12/992,751 US9669061B2 (en) | 2008-05-14 | 2009-05-05 | Medicinal agent exhibiting antiprotozoal activity to Trichomonas vaginalis in an in-vitro model system |
EP09746834.2A EP2305279B1 (en) | 2008-05-14 | 2009-05-05 | Conifer green needle complex for use in treating a trichomoniasis infection of the urogenital tract. |
AU2009247064A AU2009247064B9 (en) | 2008-05-14 | 2009-05-05 | Medicinal agent exhibiting antiprotozoal activity to Trichomonas vaginalis in an in-vitro model system |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU2008119139/15A RU2366442C1 (ru) | 2008-05-14 | 2008-05-14 | Лекарственное средство, обладающее противопротозойной активностью в отношении trichomonas vaginalis в модельной системе in vitro |
RU2008119139 | 2008-05-14 |
Publications (1)
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WO2009139665A1 true WO2009139665A1 (ru) | 2009-11-19 |
Family
ID=41166438
Family Applications (1)
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PCT/RU2009/000215 WO2009139665A1 (ru) | 2008-05-14 | 2009-05-05 | Лекарственное средство, обладающее противопротозойной активностью в отношении тriсhоmоnаs vаginаlis в модельной системе in vitrо |
Country Status (6)
Country | Link |
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US (1) | US9669061B2 (ru) |
EP (1) | EP2305279B1 (ru) |
AU (1) | AU2009247064B9 (ru) |
MY (1) | MY180493A (ru) |
RU (1) | RU2366442C1 (ru) |
WO (1) | WO2009139665A1 (ru) |
Families Citing this family (1)
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RU2678965C1 (ru) * | 2017-11-30 | 2019-02-05 | Вагиф Султанович Султанов | Лекарственное средство, фармацевтическая композиция, способ лечения урогенитальных заболеваний организма человека, вызванных микроорганизмами вида Mycoplasma hominis |
Citations (3)
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RU2320319C1 (ru) | 2006-11-07 | 2008-03-27 | Вадим Владимирович Абрамов | Суппозитории вагинальные |
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RU2021803C1 (ru) | 1991-01-11 | 1994-10-30 | Государственная научно-производственная фирма "Алата" | Крем для кожи рук |
EP0565313B1 (en) * | 1992-04-02 | 1997-07-30 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | Method to improve taste preference of pine extract, and orally administrable product obtained thereby |
RU2040266C1 (ru) * | 1992-12-29 | 1995-07-25 | Товарищество с ограниченной ответственностью "Фитолон" | Способ получения биологически активного средства из хвои |
RU2041646C1 (ru) * | 1992-12-30 | 1995-08-20 | Красноярская государственная технологическая академия | Способ получения продуктов из хвойной древесной зелени |
CN1102100A (zh) * | 1993-10-29 | 1995-05-03 | 中国林业科学研究院林产化学工业研究所 | 针叶叶绿素-胡萝卜素软膏制备方法 |
WO2004087203A2 (en) * | 2003-04-02 | 2004-10-14 | Coley Pharmaceutical Group, Ltd. | Immunostimulatory nucleic acid oil-in-water formulations for topical application |
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2008
- 2008-05-14 RU RU2008119139/15A patent/RU2366442C1/ru active
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2009
- 2009-05-05 MY MYPI2010005314A patent/MY180493A/en unknown
- 2009-05-05 AU AU2009247064A patent/AU2009247064B9/en not_active Ceased
- 2009-05-05 EP EP09746834.2A patent/EP2305279B1/en not_active Not-in-force
- 2009-05-05 WO PCT/RU2009/000215 patent/WO2009139665A1/ru active Application Filing
- 2009-05-05 US US12/992,751 patent/US9669061B2/en active Active
Patent Citations (3)
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RU2208950C2 (ru) * | 2001-08-07 | 2003-07-27 | Некрасова Валерия Борисовна | Биологически активная добавка к пище |
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Also Published As
Publication number | Publication date |
---|---|
MY180493A (en) | 2020-11-30 |
US9669061B2 (en) | 2017-06-06 |
AU2009247064B2 (en) | 2015-07-30 |
AU2009247064B9 (en) | 2015-11-19 |
EP2305279B1 (en) | 2016-04-20 |
EP2305279A1 (en) | 2011-04-06 |
RU2366442C1 (ru) | 2009-09-10 |
US20110189321A1 (en) | 2011-08-04 |
EP2305279A4 (en) | 2012-10-24 |
AU2009247064A1 (en) | 2009-11-19 |
AU2009247064A2 (en) | 2011-01-06 |
AU2009247064A9 (en) | 2015-11-19 |
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