WO2009132220A3 - Isoprene synthase variants for improved microbial production of isoprene - Google Patents

Isoprene synthase variants for improved microbial production of isoprene Download PDF

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Publication number
WO2009132220A3
WO2009132220A3 PCT/US2009/041581 US2009041581W WO2009132220A3 WO 2009132220 A3 WO2009132220 A3 WO 2009132220A3 US 2009041581 W US2009041581 W US 2009041581W WO 2009132220 A3 WO2009132220 A3 WO 2009132220A3
Authority
WO
WIPO (PCT)
Prior art keywords
isoprene
present
microbial production
synthase variants
improved microbial
Prior art date
Application number
PCT/US2009/041581
Other languages
French (fr)
Other versions
WO2009132220A2 (en
WO2009132220A9 (en
Inventor
Marguerite A. Cervin
Gregory M. Whited
Andrei Miasnikov
Caroline M. Peres
Walter Weyler
Derek H. Wells
Richard R. Bott
Original Assignee
Danisco Us Inc.
The Goodyear Tire & Rubber Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc., The Goodyear Tire & Rubber Company filed Critical Danisco Us Inc.
Priority to AU2009240505A priority Critical patent/AU2009240505B2/en
Priority to RU2010147659/10A priority patent/RU2516343C2/en
Priority to CA2722440A priority patent/CA2722440A1/en
Priority to EP09735440A priority patent/EP2279251A2/en
Priority to BRPI0911667A priority patent/BRPI0911667A2/en
Priority to JP2011506460A priority patent/JP2011518564A/en
Priority to CN2009801234600A priority patent/CN102171335A/en
Priority to MX2010011706A priority patent/MX2010011706A/en
Publication of WO2009132220A2 publication Critical patent/WO2009132220A2/en
Publication of WO2009132220A3 publication Critical patent/WO2009132220A3/en
Publication of WO2009132220A9 publication Critical patent/WO2009132220A9/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03027—Isoprene synthase (4.2.3.27)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
PCT/US2009/041581 2008-04-23 2009-04-23 Isoprene synthase variants for improved microbial production of isoprene WO2009132220A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
AU2009240505A AU2009240505B2 (en) 2008-04-23 2009-04-23 Isoprene synthase variants for improved microbial production of isoprene
RU2010147659/10A RU2516343C2 (en) 2008-04-23 2009-04-23 Versions of isoprene synthase applied for improvement of isoprene production by microorganisms
CA2722440A CA2722440A1 (en) 2008-04-23 2009-04-23 Isoprene synthase variants for improved microbial production of isoprene
EP09735440A EP2279251A2 (en) 2008-04-23 2009-04-23 Isoprene synthase variants for improved microbial production of isoprene
BRPI0911667A BRPI0911667A2 (en) 2008-04-23 2009-04-23 isoprene synthase variant for enhanced microbial isoprene production
JP2011506460A JP2011518564A (en) 2008-04-23 2009-04-23 Isoprene synthase mutants for isoprene production by improved microorganisms
CN2009801234600A CN102171335A (en) 2008-04-23 2009-04-23 Isoprene synthase variants for improved microbial production of isoprene
MX2010011706A MX2010011706A (en) 2008-04-23 2009-04-23 Isoprene synthase variants for improved microbial production of isoprene.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12533608P 2008-04-23 2008-04-23
US61/125,336 2008-04-23

Publications (3)

Publication Number Publication Date
WO2009132220A2 WO2009132220A2 (en) 2009-10-29
WO2009132220A3 true WO2009132220A3 (en) 2010-01-21
WO2009132220A9 WO2009132220A9 (en) 2010-05-06

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/041581 WO2009132220A2 (en) 2008-04-23 2009-04-23 Isoprene synthase variants for improved microbial production of isoprene

Country Status (14)

Country Link
US (3) US8173410B2 (en)
EP (1) EP2279251A2 (en)
JP (2) JP2011518564A (en)
KR (1) KR20110020234A (en)
CN (1) CN102171335A (en)
AU (1) AU2009240505B2 (en)
BR (1) BRPI0911667A2 (en)
CA (1) CA2722440A1 (en)
MX (1) MX2010011706A (en)
MY (1) MY153871A (en)
RU (2) RU2516343C2 (en)
SG (2) SG165884A1 (en)
WO (1) WO2009132220A2 (en)
ZA (2) ZA201007564B (en)

Cited By (4)

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US8916370B2 (en) 2008-04-23 2014-12-23 Danisco Us Inc. Isoprene synthase variants for improved microbial production of isoprene
US9163263B2 (en) 2012-05-02 2015-10-20 The Goodyear Tire & Rubber Company Identification of isoprene synthase variants with improved properties for the production of isoprene
US9175313B2 (en) 2009-04-23 2015-11-03 Danisco Us Inc. Three-dimensional structure of isoprene synthase and its use thereof for generating variants
US9273298B2 (en) 2010-10-27 2016-03-01 Danisco Us Inc. Isoprene synthase variants for improved production of isoprene

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WO2010031077A1 (en) * 2008-09-15 2010-03-18 Danisco Us Inc. Increased isoprene production using mevalonate kinase and isoprene synthase
EP2334788A1 (en) * 2008-09-15 2011-06-22 Danisco US Inc. Increased isoprene production using the archaeal lower mevalonate pathway
US8470581B2 (en) * 2008-09-15 2013-06-25 Danisco Us Inc. Reduction of carbon dioxide emission during isoprene production by fermentation
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