WO2009066761A1 - エステル結合を含む非天然タンパク質の製造方法 - Google Patents

エステル結合を含む非天然タンパク質の製造方法 Download PDF

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Publication number
WO2009066761A1
WO2009066761A1 PCT/JP2008/071221 JP2008071221W WO2009066761A1 WO 2009066761 A1 WO2009066761 A1 WO 2009066761A1 JP 2008071221 W JP2008071221 W JP 2008071221W WO 2009066761 A1 WO2009066761 A1 WO 2009066761A1
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WO
WIPO (PCT)
Prior art keywords
ester bond
hydroxy acid
natural protein
production
aminoacyl
Prior art date
Application number
PCT/JP2008/071221
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English (en)
French (fr)
Inventor
Shigeyuki Yokoyama
Kensaku Sakamoto
Tatsuo Yanagisawa
Takahito Mukai
Takatsugu Kobayashi
Original Assignee
Riken
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Riken filed Critical Riken
Priority to US12/744,322 priority Critical patent/US8785152B2/en
Priority to JP2009542600A priority patent/JP5419220B2/ja
Priority to EP08851711.5A priority patent/EP2221370B1/en
Publication of WO2009066761A1 publication Critical patent/WO2009066761A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

　リボソームにおける生体内翻訳系を用いて、ポリペプチド主鎖に少なくとも１つのエステル結合を含む非天然タンパク質を合成する。（ａ）α－ヒドロキシ酸を活性化しうるアミノアシルｔＲＮＡ合成酵素と、（ｂ）前記アミノアシルｔＲＮＡ合成酵素の存在下において前記α－ヒドロキシ酸と結合可能なサプレッサーｔＲＮＡと、（ｃ）所望の位置にナンセンス変異又はフレームシフト変異を受けた所望のタンパク質をコードする遺伝子と、を前記α－ヒドロキシ酸の存在下に細胞内又は細胞抽出液内で発現させる。
PCT/JP2008/071221 2007-11-22 2008-11-21 エステル結合を含む非天然タンパク質の製造方法 WO2009066761A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US12/744,322 US8785152B2 (en) 2007-11-22 2008-11-21 Process for production of non-natural protein having ester bond therein
JP2009542600A JP5419220B2 (ja) 2007-11-22 2008-11-21 エステル結合を含む非天然タンパク質の製造方法
EP08851711.5A EP2221370B1 (en) 2007-11-22 2008-11-21 Process for production of non-natural protein having ester bond therein

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-302452 2007-11-22
JP2007302452 2007-11-22

Publications (1)

Publication Number Publication Date
WO2009066761A1 true WO2009066761A1 (ja) 2009-05-28

Family

ID=40667583

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/071221 WO2009066761A1 (ja) 2007-11-22 2008-11-21 エステル結合を含む非天然タンパク質の製造方法

Country Status (4)

Country Link
US (1) US8785152B2 (ja)
EP (1) EP2221370B1 (ja)
JP (1) JP5419220B2 (ja)
WO (1) WO2009066761A1 (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019533999A (ja) * 2016-10-14 2019-11-28 ヨーロピアン モレキュラー バイオロジー ラボラトリーＥｕｒｏｐｅａｎ Ｍｏｌｅｃｕｌａｒ Ｂｉｏｌｏｇｙ Ｌａｂｏｒａｔｏｒｙ 直交（ｏｒｔｈｏｇｏｎａｌ）用途の古細菌ピロリジルｔＲＮＡ合成酵素
WO2020045656A1 (ja) 2018-08-31 2020-03-05 国立研究開発法人理化学研究所 ピロリシルｔＲＮＡ合成酵素

Families Citing this family (7)

* Cited by examiner, † Cited by third party
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US20130183761A1 (en) * 2010-09-24 2013-07-18 North Carolina State University Methods for Incorporating Unnatural Amino Acids in Eukaryotic Cells
WO2017161183A1 (en) 2016-03-16 2017-09-21 The Regents Of The University Of California Covalent peptide binders
AU2019231893A1 (en) * 2018-03-08 2020-10-29 The Regents Of The University Of California Bioreactive compositions and methods of use thereof
CN111718920B (zh) * 2019-03-19 2021-05-07 宁波鲲鹏生物科技有限公司 在蛋白中高效引入赖氨酸衍生物的氨酰基-tRNA合成酶
WO2023031445A2 (en) 2021-09-06 2023-03-09 Veraxa Biotech Gmbh Novel aminoacyl-trna synthetase variants for genetic code expansion in eukaryotes
EP4186529A1 (en) 2021-11-25 2023-05-31 Veraxa Biotech GmbH Improved antibody-payload conjugates (apcs) prepared by site-specific conjugation utilizing genetic code expansion
WO2023094525A1 (en) 2021-11-25 2023-06-01 Veraxa Biotech Gmbh Improved antibody-payload conjugates (apcs) prepared by site-specific conjugation utilizing genetic code expansion

Citations (2)

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WO2007015527A1 (ja) * 2005-08-02 2007-02-08 Riken ｔＲＮＡ合成方法、核酸、アミノアシルｔＲＮＡ合成方法及び非天然型アミノ酸組み込み蛋白質の製造方法
JP2007243574A (ja) 2006-03-08 2007-09-20 Konica Minolta Business Technologies Inc タイムスタンプ取得装置、タイムスタンプ取得方法及びタイムスタンプ取得プログラム

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US20060166319A1 (en) * 2004-08-13 2006-07-27 Chan Michael K Charging tRNA with pyrrolysine
WO2007099854A1 (ja) * 2006-02-22 2007-09-07 Riken サプレッサーｔＲＮＡの合成方法、ＤＮＡ構築物及びそれを用いた非天然型アミノ酸組み込みタンパク質の製造
JP4440225B2 (ja) 2006-02-28 2010-03-24 パナソニック株式会社 プラズマディスプレイパネルの製造方法
BRPI0817212A2 (pt) 2007-09-20 2014-10-14 Riken Pirrolisil-trna sintetase mutante, e método para a produção de proteína tendo aminoácido não-natural integrado nela por sua utilização

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
WO2007015527A1 (ja) * 2005-08-02 2007-02-08 Riken ｔＲＮＡ合成方法、核酸、アミノアシルｔＲＮＡ合成方法及び非天然型アミノ酸組み込み蛋白質の製造方法
JP2007037445A (ja) 2005-08-02 2007-02-15 Institute Of Physical & Chemical Research ｔＲＮＡ合成方法、核酸、アミノアシルｔＲＮＡ合成方法及び非天然型アミノ酸組み込み蛋白質の製造方法
JP2007243574A (ja) 2006-03-08 2007-09-20 Konica Minolta Business Technologies Inc タイムスタンプ取得装置、タイムスタンプ取得方法及びタイムスタンプ取得プログラム

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019533999A (ja) * 2016-10-14 2019-11-28 ヨーロピアン モレキュラー バイオロジー ラボラトリーＥｕｒｏｐｅａｎ Ｍｏｌｅｃｕｌａｒ Ｂｉｏｌｏｇｙ Ｌａｂｏｒａｔｏｒｙ 直交（ｏｒｔｈｏｇｏｎａｌ）用途の古細菌ピロリジルｔＲＮＡ合成酵素
JP7277361B2 (ja) 2016-10-14 2023-05-18 ヨーロピアン モレキュラー バイオロジー ラボラトリー 直交（ｏｒｔｈｏｇｏｎａｌ）用途の古細菌ピロリジルｔＲＮＡ合成酵素
WO2020045656A1 (ja) 2018-08-31 2020-03-05 国立研究開発法人理化学研究所 ピロリシルｔＲＮＡ合成酵素

Also Published As

Publication number Publication date
EP2221370B1 (en) 2014-04-16
JPWO2009066761A1 (ja) 2011-04-07
JP5419220B2 (ja) 2014-02-19
US20110136168A1 (en) 2011-06-09
EP2221370A4 (en) 2011-01-05
US8785152B2 (en) 2014-07-22
EP2221370A1 (en) 2010-08-25

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