WO2009017014A1 - マイクロ流路形成体を利用したマイクロビーズアレイ用チップ、マイクロビーズアレイ及びこれらを用いた被検物質を検出する方法 - Google Patents

マイクロ流路形成体を利用したマイクロビーズアレイ用チップ、マイクロビーズアレイ及びこれらを用いた被検物質を検出する方法 Download PDF

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WO2009017014A1
WO2009017014A1 PCT/JP2008/063241 JP2008063241W WO2009017014A1 WO 2009017014 A1 WO2009017014 A1 WO 2009017014A1 JP 2008063241 W JP2008063241 W JP 2008063241W WO 2009017014 A1 WO2009017014 A1 WO 2009017014A1
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microchannel
microbead
forming member
substrate
chip
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PCT/JP2008/063241
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English (en)
French (fr)
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Yasuhiro Sasuga
Yoshie Harada
Osamu Ohara
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Kazusa Dna Research Institute Foundation
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00373—Hollow needles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00457—Dispensing or evacuation of the solid phase support
    • B01J2219/00459—Beads
    • B01J2219/00466—Beads in a slurry
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00457—Dispensing or evacuation of the solid phase support
    • B01J2219/00459—Beads
    • B01J2219/00468—Beads by manipulation of individual beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/005—Beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/0054—Means for coding or tagging the apparatus or the reagents
    • B01J2219/00572—Chemical means
    • B01J2219/00576—Chemical means fluorophore
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00657—One-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00725—Peptides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099—Characterised by type of test elements
    • G01N2035/00158—Elements containing microarrays, i.e. "biochip"

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Fluid Mechanics (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Optical Measuring Cells (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

本発明は、透明な材質からなる表面が平らな基板（１）、該基板と協働してマイクロビーズを含む溶液を流すためのマイクロ流路を形成するように表面が前記基板の表面に密着して設けられる透明な材質からなるマイクロ流路形成体（２）、並行する複数の直線部分を有する該マイクロ流路形成体の表面に形成された溝（３）、少なくとも一つのマイクロ流路の下流部において前記溶液が流れる隙間を有するように該溝の内部に突出して設けられ先頭のマイクロビーズの移動を阻止して後続のマイクロビーズを複数個整列して貯留させるための突起部（４）、前記流路の上流部に設けられ前記基板及び前記マイクロ流路形成体へ溶液を注入する注入口（５）、前記流路の下流部に設けられ前記基板及び前記マイクロ流路形成体から溶液を排出する排出口（６）を備えたマイクロビーズアレイ用チップに関する。
PCT/JP2008/063241 2007-07-27 2008-07-24 マイクロ流路形成体を利用したマイクロビーズアレイ用チップ、マイクロビーズアレイ及びこれらを用いた被検物質を検出する方法 WO2009017014A1 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007195476A JP2009031126A (ja) 2007-07-27 2007-07-27 マイクロ流路形成体を利用したマイクロビーズアレイ用チップ、マイクロビーズアレイ及びこれらを用いた被検物質を検出する方法。
JP2007-195476 2007-07-27

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2336781A1 (en) 2009-12-16 2011-06-22 Sony Corporation Cell for testing microbeads and method of analyzing microbeads
CN111665237A (zh) * 2019-03-08 2020-09-15 上海索昕生物科技有限公司 一种均相化学发光检测方法及其应用
CN113769806A (zh) * 2021-10-21 2021-12-10 暨南大学 一种微流控芯片及其制备方法与结合两步微球竞争法在c-反应蛋白检测中的应用

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105209917A (zh) * 2013-03-12 2015-12-30 密歇根大学董事会 用于免疫印迹的微流体装置
EP3755788A4 (en) * 2018-02-19 2021-12-22 National Research Council of Canada MICROFLUIDIC DEVICE FOR CULTIVATING CELLS, INCLUDING A BIOPAROI, A BALL BED AND A BIOINTERFACE, AND METHODS FOR MODELING THE SAID BIOINTERFACE
CN108993621A (zh) * 2018-07-09 2018-12-14 浙江大学 一种用于数字酶联免疫检测的微小室阵列微流控芯片及方法

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JP2003536058A (ja) * 2000-06-05 2003-12-02 カリフォルニア・インスティテュート・オブ・テクノロジー 集積アクティブフラックスマイクロ流体デバイスおよび方法
JP2006201091A (ja) * 2005-01-21 2006-08-03 Sumitomo Bakelite Co Ltd 捕捉ビーズ用マイクロ粒子およびそれを用いた捕捉ビーズならびにバイオチップ
JP2006284440A (ja) * 2005-04-01 2006-10-19 Ymc Co Ltd クロマトグラフィー用チップの製造方法
JP2007107913A (ja) * 2005-10-11 2007-04-26 Canon Inc 反応素子及びその製造方法
JP2007108075A (ja) * 2005-10-14 2007-04-26 Sharp Corp 分析用マイクロチップ及びこれを用いた分析用マイクロチップ装置並びにその再利用方法
JP2007163459A (ja) * 2005-11-18 2007-06-28 Sharp Corp 分析用マイクロチップ
WO2007074756A1 (ja) * 2005-12-26 2007-07-05 Institute Of Microchemical Technology 免疫分析マイクロチップ、免疫分析用キット及び免疫分析方法

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003536058A (ja) * 2000-06-05 2003-12-02 カリフォルニア・インスティテュート・オブ・テクノロジー 集積アクティブフラックスマイクロ流体デバイスおよび方法
JP2006201091A (ja) * 2005-01-21 2006-08-03 Sumitomo Bakelite Co Ltd 捕捉ビーズ用マイクロ粒子およびそれを用いた捕捉ビーズならびにバイオチップ
JP2006284440A (ja) * 2005-04-01 2006-10-19 Ymc Co Ltd クロマトグラフィー用チップの製造方法
JP2007107913A (ja) * 2005-10-11 2007-04-26 Canon Inc 反応素子及びその製造方法
JP2007108075A (ja) * 2005-10-14 2007-04-26 Sharp Corp 分析用マイクロチップ及びこれを用いた分析用マイクロチップ装置並びにその再利用方法
JP2007163459A (ja) * 2005-11-18 2007-06-28 Sharp Corp 分析用マイクロチップ
WO2007074756A1 (ja) * 2005-12-26 2007-07-05 Institute Of Microchemical Technology 免疫分析マイクロチップ、免疫分析用キット及び免疫分析方法

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2336781A1 (en) 2009-12-16 2011-06-22 Sony Corporation Cell for testing microbeads and method of analyzing microbeads
CN111665237A (zh) * 2019-03-08 2020-09-15 上海索昕生物科技有限公司 一种均相化学发光检测方法及其应用
CN113769806A (zh) * 2021-10-21 2021-12-10 暨南大学 一种微流控芯片及其制备方法与结合两步微球竞争法在c-反应蛋白检测中的应用
CN113769806B (zh) * 2021-10-21 2022-09-27 暨南大学 一种微流控芯片及其制备方法与结合两步微球竞争法在c-反应蛋白检测中的应用

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