WO2009005155A1 - 間葉系幹細胞の増殖能・分化能維持方法 - Google Patents

間葉系幹細胞の増殖能・分化能維持方法 Download PDF

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Publication number
WO2009005155A1
WO2009005155A1 PCT/JP2008/062239 JP2008062239W WO2009005155A1 WO 2009005155 A1 WO2009005155 A1 WO 2009005155A1 JP 2008062239 W JP2008062239 W JP 2008062239W WO 2009005155 A1 WO2009005155 A1 WO 2009005155A1
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msc
differentiation ability
proliferation
stem cell
mesenchymal stem
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PCT/JP2008/062239
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English (en)
French (fr)
Inventor
Masahiro Go
Chiemi Takenaka
Hajime Ohgushi
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National Institute Of Advanced Industrial Science And Technology
Stem Cell Sciences Kk
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Publication of WO2009005155A1 publication Critical patent/WO2009005155A1/ja

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/602—Sox-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/605—Nanog

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Rheumatology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

【課題】ヒト成人骨髄由来MSCの増殖能・分化能を維持する方法、或いは増殖能・分化能を維持した間葉系幹細胞、さらには間葉系幹細胞に作用する薬物のスクリーニング方法を開発することを目的とし、間葉系幹細胞(MSC)、特にヒト成人骨髄由来MSCの増殖能・分化能を維持する。 【解決手段】間葉系幹細胞(MSC)、特にヒト成人骨髄由来MSCの増殖能・分化能を維持する方法を提供する。Sox2あるいはNanog遺伝子を導入することで強制的・安定的に遺伝子発現を誘導すること、あるいはSox2遺伝子を導入することと成長因子であるbFGFの培地への添加を併用することにより、MSCの増殖能・分化能を維持することができる。
PCT/JP2008/062239 2007-07-05 2008-07-05 間葉系幹細胞の増殖能・分化能維持方法 WO2009005155A1 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007177767A JP2009011254A (ja) 2007-07-05 2007-07-05 間葉系幹細胞の増殖能・分化能維持方法
JP2007-177767 2007-07-05

Publications (1)

Publication Number Publication Date
WO2009005155A1 true WO2009005155A1 (ja) 2009-01-08

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JP (1) JP2009011254A (ja)
WO (1) WO2009005155A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108603167A (zh) * 2015-09-15 2018-09-28 思特科技公司 使用其中引入nanog的源自羊水胎儿的间充质干细胞制备用于促进毛发生长的组合物的方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110191714A (zh) * 2017-01-11 2019-08-30 思特科技公司 制备用于毛发生长的组合物的方法，该组合物包含从引入了nanog的羊水中胎儿衍生间充质干细胞获得的外泌体

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005110565A (ja) * 2003-10-07 2005-04-28 Nobuya Yamanaka 分化多能性維持剤
WO2007010858A1 (ja) * 2005-07-15 2007-01-25 Kyoto University 骨格筋組織由来の単一細胞よりクローン化した多能性幹細胞

Patent Citations (2)

* Cited by examiner, † Cited by third party
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JP2005110565A (ja) * 2003-10-07 2005-04-28 Nobuya Yamanaka 分化多能性維持剤
WO2007010858A1 (ja) * 2005-07-15 2007-01-25 Kyoto University 骨格筋組織由来の単一細胞よりクローン化した多能性幹細胞

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BOYER L.A. ET AL.: "Core transcriptional regulatory circuitry in human embryonic stem cells", CELL, vol. 122, no. 6, 2005, pages 947 - 956, XP002412923 *
GO M.J. ET AL.: "Forced expression of Sox2 or Nanog in human bone marrow derived mesenchymal stem cells maintains their expansion and differentiation capabilities", EXP. CELL RES., vol. 314, no. 5, pages 1147 - 1154, XP022493853 *
GUILLOT P.V. ET AL.: "Human first-trimester fetal MSC express pluripotency markers and grow faster and have longer telomeres than adult MSC", STEM CELLS, vol. 25, no. 3, pages 646 - 654 *
ILANCHERAN S. ET AL.: "Stem cells derived from human fetal membranes display multilineage differentiation potential", BIOL. REPROD., vol. 77, no. 3, pages 577 - 588 *
LOH Y.H. ET AL.: "The Oct4 and Nanog transcription network regulation pluripotency in mouse embryonic stem cells", NAT. GENET., vol. 38, no. 4, 2006, pages 431 - 440 *
OGUSHI H.: "Hone Saisei o Mezashita Kan'yokei Kansaibo no Fukatsuka Gijutsu -Hito Kan'yokei Donyu-", INFLAMM. REGEN., vol. 28, no. 4, 1 July 2008 (2008-07-01), pages 290 + ABSTR. NO. S4-6 *
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108603167A (zh) * 2015-09-15 2018-09-28 思特科技公司 使用其中引入nanog的源自羊水胎儿的间充质干细胞制备用于促进毛发生长的组合物的方法
US20180362606A1 (en) * 2015-09-15 2018-12-20 Stemlab Inc. Method for preparing composition for promoting hair growth using nanog-introduced mesenchymal stem cells derived from fetus in amniotic fluid
US10640541B2 (en) * 2015-09-15 2020-05-05 Stemlab Inc. Method for preparing composition for promoting hair growth using Nanog-introduced mesenchymal stem cells derived from fetus in amniotic fluid
CN108603167B (zh) * 2015-09-15 2022-01-21 思特科技公司 使用nanog引入的源自羊水胎儿的间充质干细胞制备用于促进毛发生长的组合物的方法

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