WO2011025179A3 - Method of inducing the differentiation of human embryonic stem cells into mesenchymal stem cells - Google Patents

Method of inducing the differentiation of human embryonic stem cells into mesenchymal stem cells Download PDF

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Publication number
WO2011025179A3
WO2011025179A3 PCT/KR2010/005471 KR2010005471W WO2011025179A3 WO 2011025179 A3 WO2011025179 A3 WO 2011025179A3 KR 2010005471 W KR2010005471 W KR 2010005471W WO 2011025179 A3 WO2011025179 A3 WO 2011025179A3
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Prior art keywords
stem cells
human embryonic
mesenchymal stem
inducing
embryonic stem
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PCT/KR2010/005471
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French (fr)
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WO2011025179A2 (en
Inventor
Yong-Mahn Han
Tran-Ngoc Tung
Sang-Wook Park
Mi-Jin Jang
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Korea Advanced Institute Of Science And Technology
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Publication of WO2011025179A2 publication Critical patent/WO2011025179A2/en
Publication of WO2011025179A3 publication Critical patent/WO2011025179A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Disclosed is a method for inducing human embryonic stem cells to differentiate into mesenchymal stem cells, using an optimal medium. Human embryonic stem cells are induced differentiate into mesodermal progenitors by activating TGF-β (Transforming growth factor-β), WNT and BMP (Bone morphogenic protein) signaling pathways and further into mesenchymal stem cells by treating with bFGF. The method is effective for producing mesenchymal stem cells, useful cell-based therapies, on a large scale.
PCT/KR2010/005471 2009-08-24 2010-08-18 Method of inducing the differentiation of human embryonic stem cells into mesenchymal stem cells WO2011025179A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2009-0078088 2009-08-24
KR1020090078088A KR101193541B1 (en) 2009-08-24 2009-08-24 Method of inducing differentiation of human embryonic stem cells into mesenchymal stem cells

Publications (2)

Publication Number Publication Date
WO2011025179A2 WO2011025179A2 (en) 2011-03-03
WO2011025179A3 true WO2011025179A3 (en) 2011-06-30

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2010/005471 WO2011025179A2 (en) 2009-08-24 2010-08-18 Method of inducing the differentiation of human embryonic stem cells into mesenchymal stem cells

Country Status (2)

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KR (1) KR101193541B1 (en)
WO (1) WO2011025179A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111876380B (en) * 2020-08-04 2022-06-28 浙江大学 Induction medium for differentiating human embryonic stem cells into mesenchymal stem cells

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2377923A1 (en) 2006-04-14 2011-10-19 Advanced Cell Technology, Inc. Hemangio-colony forming cells
IL231205B (en) * 2011-08-29 2021-05-31 Inst Nat Sante Rech Med Method for preparing induced paraxial mesoderm progenitor (ipam) cells and their use
JP6570833B2 (en) * 2011-08-29 2019-09-04 アンスティトゥー ナショナル ドゥ ラ サンテ エ ドゥ ラ レシェルシュ メディカル（イエヌエスエエールエム） Method for preparing artificial paraxial mesoderm progenitor (iPAM) cells and use thereof
EP2872619B1 (en) * 2012-07-11 2018-02-14 Imstem Biotechnology Inc. Mesenchymal-like stem cells derived from human embryonic stem cells, methods and uses thereof
WO2017069376A1 (en) * 2015-10-20 2017-04-27 전남대학교병원 Composition for treating inflammatory diseases, containing ikkε inhibitor
CN107937338B (en) * 2017-11-30 2021-08-03 中山大学 Mesoderm lineage mesenchymal stem cell from pluripotent stem cell and preparation method thereof
JP7376760B2 (en) * 2018-03-15 2023-11-09 国立大学法人京都大学 Step-by-step production method from pluripotent stem cells to various types of cells
CN113018316A (en) * 2019-12-24 2021-06-25 厦门大学 Application of mesenchymal stem cells derived from pluripotent stem cells in repairing skin injury
CN111849885B (en) * 2020-08-04 2022-07-01 浙江大学 Method for inducing differentiation of human embryonic stem cells into mesenchymal stem cells

Citations (2)

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Publication number Priority date Publication date Assignee Title
WO2008094597A2 (en) * 2007-01-30 2008-08-07 University Of Georgia Research Foundation, Inc. Early mesoderm cells, a stable population of mesendoderm cells that has utility for generation of endoderm and mesoderm lineages and multipotent migratory cells (mmc)
KR20090089206A (en) * 2008-02-18 2009-08-21 한국과학기술원 Method for inducing the defferentiation of embryonic stem cells into hemangioblast

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008094597A2 (en) * 2007-01-30 2008-08-07 University Of Georgia Research Foundation, Inc. Early mesoderm cells, a stable population of mesendoderm cells that has utility for generation of endoderm and mesoderm lineages and multipotent migratory cells (mmc)
KR20090089206A (en) * 2008-02-18 2009-08-21 한국과학기술원 Method for inducing the defferentiation of embryonic stem cells into hemangioblast

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JOHANSSON, B.M. ET AL.: "Evidence for involvement of Activin A and bone morphogenetic protein 4 in mammalian mesoderm and hematopoietic development.", MOLECULAR AND CELLULAR BIOLOGY., vol. 15, no. 1, 1995, pages 141 - 151 *
WILLEMS, E. ET AL.: "Patterning of mouse embryonic stem cell-derived pan-mesoderm by Activin A/Nodal and Bmp4 signaling requires fibroblast growth factor activity.", DIFFERENTIATION., vol. 76, 2008, pages 745 - 759 *
ZHANG, P. ET AL.: "Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells.", BLOOD., vol. 111, 2008, pages 1933 - 1941 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111876380B (en) * 2020-08-04 2022-06-28 浙江大学 Induction medium for differentiating human embryonic stem cells into mesenchymal stem cells

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Publication number Publication date
WO2011025179A2 (en) 2011-03-03
KR20110020468A (en) 2011-03-03
KR101193541B1 (en) 2012-10-22

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