WO2008130053A1 - プロテインｇとアビジン類との融合タンパク質 - Google Patents

プロテインｇとアビジン類との融合タンパク質 Download PDF

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Publication number
WO2008130053A1
WO2008130053A1 PCT/JP2008/057866 JP2008057866W WO2008130053A1 WO 2008130053 A1 WO2008130053 A1 WO 2008130053A1 JP 2008057866 W JP2008057866 W JP 2008057866W WO 2008130053 A1 WO2008130053 A1 WO 2008130053A1
Authority
WO
WIPO (PCT)
Prior art keywords
protein
avidin
fused
antibody
same
Prior art date
Application number
PCT/JP2008/057866
Other languages
English (en)
French (fr)
Inventor
Yuichiro Ishikawa
Original Assignee
Synthera Technologies Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Synthera Technologies Co., Ltd. filed Critical Synthera Technologies Co., Ltd.
Priority to JP2009510871A priority Critical patent/JP5211041B2/ja
Publication of WO2008130053A1 publication Critical patent/WO2008130053A1/ja

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/465—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from birds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

【課題】標的抗原の検出法に用いる標識化抗体（抗体に標識物質を固定した抗体）を作製するにあたり、カップリング法等の化学結合法を用いる必要がなく、かつ非特異結合を十分に抑制することが可能な、アンカー物質を提供する。 【解決手段】本発明の融合タンパク質は、プロテインＧ又はその一部からなるタンパク質と、アビジン類又はその一部からなるタンパク質とを含む融合タンパク質である。本発明の標識化抗体は、IgG抗体、本発明の融合タンパク質、及びビオチン化標識物質を含んでなるものである。
PCT/JP2008/057866 2007-04-23 2008-04-23 プロテインｇとアビジン類との融合タンパク質 WO2008130053A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2009510871A JP5211041B2 (ja) 2007-04-23 2008-04-23 プロテインｇとアビジン類との融合タンパク質

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-112779 2007-04-23
JP2007112779 2007-04-23

Publications (1)

Publication Number Publication Date
WO2008130053A1 true WO2008130053A1 (ja) 2008-10-30

Family

ID=39875572

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/057866 WO2008130053A1 (ja) 2007-04-23 2008-04-23 プロテインｇとアビジン類との融合タンパク質

Country Status (2)

Country Link
JP (1) JP5211041B2 (ja)
WO (1) WO2008130053A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5647599B2 (ja) * 2009-03-02 2015-01-07 日本たばこ産業株式会社 生物学的試料中の物質を検出する方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63503032A (ja) * 1986-03-21 1988-11-10 フアーマシア・アー・ベー 免疫グロブリン結合性タンパク質の産生方法と手段
JP2003517293A (ja) * 1999-07-20 2003-05-27 セプティア， インコーポレイテッド Ｄｓｐ−１１二重特異性ｍａｐキナーゼホスファターゼ
WO2006043675A1 (ja) * 2004-10-22 2006-04-27 Cellfree Sciences Co., Ltd. 無細胞タンパク質合成用細胞抽出液及び該抽出液の調製方法。
WO2006049289A1 (ja) * 2004-11-01 2006-05-11 Tokyo Metropolitan Organization For Medical Research 標的物質の検出方法
JP2006132980A (ja) * 2004-11-02 2006-05-25 Toyobo Co Ltd タンパク質の固定化方法
WO2006062200A1 (ja) * 2004-12-09 2006-06-15 Universal Bio Research Co., Ltd. 核酸ライブラリー及びタンパク質ライブラリー

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63503032A (ja) * 1986-03-21 1988-11-10 フアーマシア・アー・ベー 免疫グロブリン結合性タンパク質の産生方法と手段
JPH08205875A (ja) * 1986-03-21 1996-08-13 Pharmacia Ab 免疫グロブリン結合性タンパク質の産生方法
JP2003517293A (ja) * 1999-07-20 2003-05-27 セプティア， インコーポレイテッド Ｄｓｐ−１１二重特異性ｍａｐキナーゼホスファターゼ
WO2006043675A1 (ja) * 2004-10-22 2006-04-27 Cellfree Sciences Co., Ltd. 無細胞タンパク質合成用細胞抽出液及び該抽出液の調製方法。
WO2006049289A1 (ja) * 2004-11-01 2006-05-11 Tokyo Metropolitan Organization For Medical Research 標的物質の検出方法
JP2006132980A (ja) * 2004-11-02 2006-05-25 Toyobo Co Ltd タンパク質の固定化方法
WO2006062200A1 (ja) * 2004-12-09 2006-06-15 Universal Bio Research Co., Ltd. 核酸ライブラリー及びタンパク質ライブラリー

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
AKERSTROM B. ET AL.: "Definition of IgG- and Albumin-binding Regions of Streptococcal Protein G", J. BIOL. CHEM., vol. 262, 1987, pages 13388 - 13391, XP002969227 *
FREITAG S. ET AL.: "Structural studies of the streptavidin binding loop", PROTEIN SCIENCE, vol. 6, 1997, pages 1157 - 1166, XP002325362 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5647599B2 (ja) * 2009-03-02 2015-01-07 日本たばこ産業株式会社 生物学的試料中の物質を検出する方法

Also Published As

Publication number Publication date
JPWO2008130053A1 (ja) 2010-07-22
JP5211041B2 (ja) 2013-06-12

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