WO2008102417A1 - 蛍光読取装置及び蛍光読取方法 - Google Patents

蛍光読取装置及び蛍光読取方法 Download PDF

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Publication number
WO2008102417A1
WO2008102417A1 PCT/JP2007/000910 JP2007000910W WO2008102417A1 WO 2008102417 A1 WO2008102417 A1 WO 2008102417A1 JP 2007000910 W JP2007000910 W JP 2007000910W WO 2008102417 A1 WO2008102417 A1 WO 2008102417A1
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WO
WIPO (PCT)
Prior art keywords
fluorescence
incidence
angle
reader
information
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2007/000910
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English (en)
French (fr)
Inventor
Tadao Sugiura
Masato Mori
Eiji Inamoto
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Japan Science and Technology Agency
Nara Institute of Science and Technology NUC
Original Assignee
Japan Science and Technology Agency
Nara Institute of Science and Technology NUC
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Publication date
Application filed by Japan Science and Technology Agency, Nara Institute of Science and Technology NUC filed Critical Japan Science and Technology Agency
Priority to US12/527,780 priority Critical patent/US8384044B2/en
Priority to JP2009500018A priority patent/JP5093522B2/ja
Publication of WO2008102417A1 publication Critical patent/WO2008102417A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy

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  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

　【課題】　本発明は，プローブ物質とターゲット物質との相互作用に関与しない蛍光物質に由来する蛍光の影響を考慮したマイクロアレイ読取装置などの蛍光読取装置を提供することを目的とする。 　【解決手段】　上記の課題は，光源（７）からの光が基板（２）へ入射する際の入射角を調整する入射角調整手段（１０）と；入射角調整手段（１０）が調整する入射角の量を制御するとともに，入射角に関する情報と，複数の入射角における蛍光強度又は蛍光像に関する情報とが入力され，複数の入射角について，入射角に関する情報からエバネッセント場の侵入長を求める手段と，求められた複数の侵入長における蛍光強度に関する情報を求める手段と，を有する制御装置（１１）；を具備する，蛍光読取装置（１２）により解決される。 　
PCT/JP2007/000910 2007-02-19 2007-08-27 蛍光読取装置及び蛍光読取方法 Ceased WO2008102417A1 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US12/527,780 US8384044B2 (en) 2007-02-19 2007-08-27 Apparatus and method for reading fluorescence
JP2009500018A JP5093522B2 (ja) 2007-02-19 2007-08-27 蛍光読取装置及び蛍光読取方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-038706 2007-02-19
JP2007038706 2007-02-19

Publications (1)

Publication Number Publication Date
WO2008102417A1 true WO2008102417A1 (ja) 2008-08-28

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2007/000910 Ceased WO2008102417A1 (ja) 2007-02-19 2007-08-27 蛍光読取装置及び蛍光読取方法

Country Status (3)

Country Link
US (1) US8384044B2 (ja)
JP (1) JP5093522B2 (ja)
WO (1) WO2008102417A1 (ja)

Cited By (8)

* Cited by examiner, † Cited by third party
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WO2012111645A1 (ja) * 2011-02-18 2012-08-23 コニカミノルタホールディングス株式会社 表面プラズモン励起増強蛍光分光法を用いた蛍光検出方法
WO2013031141A1 (ja) * 2011-08-29 2013-03-07 パナソニック株式会社 分子検出装置、分子検出方法及び分子検出用カートリッジ
JP2013061179A (ja) * 2011-09-12 2013-04-04 Konica Minolta Holdings Inc 表面プラズモン励起増強蛍光分光法用センサチップおよびそれを用いた測定方法
JP2014149295A (ja) * 2013-01-31 2014-08-21 Industry-Academic Cooperation Foundation Yonsei Univ 超高解像度光学映像装置及びこれを用いた光学映像方法
RU2590232C2 (ru) * 2012-04-02 2016-07-10 ЭКОЛАБ ЮЭсЭй ИНК. Проточная камера для флуорометра реального времени
JP2020531839A (ja) * 2017-08-21 2020-11-05 サウジ アラビアン オイル カンパニー 試料の組成を特定するための蛍光測定装置、システム、及び方法
JP2020531837A (ja) * 2017-08-21 2020-11-05 サウジ アラビアン オイル カンパニー 試料の組成を特定するための蛍光測定装置、システム、及び方法
CN112089401A (zh) * 2020-09-18 2020-12-18 中国科学院半导体研究所 多光源钙离子荧光检测方法及系统

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SG184008A1 (en) * 2010-03-12 2012-10-30 Cascade Microtech Inc System for testing semiconductors
WO2013137247A1 (ja) * 2012-03-12 2013-09-19 三菱レイヨン株式会社 蛍光検出装置及び蛍光検出方法
EP3521814B1 (en) * 2016-09-30 2022-08-03 Rigaku Corporation Wavelength-dispersive x-ray fluorescence spectrometer and x-ray fluorescence analysing method using the same
US11803018B2 (en) * 2021-01-12 2023-10-31 Hi Llc Devices, systems, and methods with a piezoelectric-driven light intensity modulator
CN113049550B (zh) * 2021-02-04 2022-05-27 浙江大学 一种基于图像重构算法的纵向层析成像方法和装置
US12309327B2 (en) * 2021-02-24 2025-05-20 General Electric Company Automated beam scan calibration, alignment, and adjustment
CN115198005B (zh) * 2022-07-04 2023-09-08 北京翔东智能科技有限公司 一种基于荧光探针法检测基因突变的方法

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JP2006105658A (ja) * 2004-10-01 2006-04-20 Fuji Photo Film Co Ltd 固定装置及び固定方法
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JP2005030906A (ja) * 2003-07-11 2005-02-03 Mitsubishi Chemicals Corp 分析用チップ及び分析方法
JP2005106711A (ja) * 2003-09-30 2005-04-21 Dainippon Printing Co Ltd 有機積層体及び有機デバイス
JP2005121796A (ja) * 2003-10-15 2005-05-12 Olympus Corp レーザー顕微鏡
JP2006038816A (ja) * 2004-07-30 2006-02-09 Nara Institute Of Science & Technology マイクロアレイ読取装置
JP2006105658A (ja) * 2004-10-01 2006-04-20 Fuji Photo Film Co Ltd 固定装置及び固定方法
JP2006317433A (ja) * 2005-04-13 2006-11-24 Fujifilm Holdings Corp 送液装置及びその送液方法並びに全反射減衰を利用した測定装置
JP2007003490A (ja) * 2005-06-27 2007-01-11 Fujifilm Holdings Corp アナライト回収装置、及び、アナライト回収方法

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012111645A1 (ja) * 2011-02-18 2012-08-23 コニカミノルタホールディングス株式会社 表面プラズモン励起増強蛍光分光法を用いた蛍光検出方法
JPWO2012111645A1 (ja) * 2011-02-18 2014-07-07 コニカミノルタ株式会社 表面プラズモン励起増強蛍光分光法を用いた蛍光検出方法
WO2013031141A1 (ja) * 2011-08-29 2013-03-07 パナソニック株式会社 分子検出装置、分子検出方法及び分子検出用カートリッジ
JP2013061179A (ja) * 2011-09-12 2013-04-04 Konica Minolta Holdings Inc 表面プラズモン励起増強蛍光分光法用センサチップおよびそれを用いた測定方法
RU2590232C2 (ru) * 2012-04-02 2016-07-10 ЭКОЛАБ ЮЭсЭй ИНК. Проточная камера для флуорометра реального времени
JP2014149295A (ja) * 2013-01-31 2014-08-21 Industry-Academic Cooperation Foundation Yonsei Univ 超高解像度光学映像装置及びこれを用いた光学映像方法
JP2020531839A (ja) * 2017-08-21 2020-11-05 サウジ アラビアン オイル カンパニー 試料の組成を特定するための蛍光測定装置、システム、及び方法
JP2020531837A (ja) * 2017-08-21 2020-11-05 サウジ アラビアン オイル カンパニー 試料の組成を特定するための蛍光測定装置、システム、及び方法
US11726038B2 (en) 2017-08-21 2023-08-15 Saudi Arabian Oil Company Determining composition of a sample
US11733163B2 (en) 2017-08-21 2023-08-22 Saudi Arabian Oil Company Determining composition of a sample
CN112089401A (zh) * 2020-09-18 2020-12-18 中国科学院半导体研究所 多光源钙离子荧光检测方法及系统
CN112089401B (zh) * 2020-09-18 2024-04-19 中国科学院半导体研究所 多光源钙离子荧光检测方法及系统

Also Published As

Publication number Publication date
JP5093522B2 (ja) 2012-12-12
JPWO2008102417A1 (ja) 2010-05-27
US20100140503A1 (en) 2010-06-10
US8384044B2 (en) 2013-02-26

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