WO2008099898A1 - ジスルフィド結合導入トランスグルタミナーゼ - Google Patents

ジスルフィド結合導入トランスグルタミナーゼ Download PDF

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Publication number
WO2008099898A1
WO2008099898A1 PCT/JP2008/052470 JP2008052470W WO2008099898A1 WO 2008099898 A1 WO2008099898 A1 WO 2008099898A1 JP 2008052470 W JP2008052470 W JP 2008052470W WO 2008099898 A1 WO2008099898 A1 WO 2008099898A1
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WO
WIPO (PCT)
Prior art keywords
transglutaminase
disulfide bond
bond introduced
bond
mutation
Prior art date
Application number
PCT/JP2008/052470
Other languages
English (en)
French (fr)
Inventor
Keiichi Yokoyama
Mototaka Suzuki
Tatsuki Kashiwagi
Eiichiro Suzuki
Masayo Date
Seiichi Taguchi
Original Assignee
Ajinomoto Co., Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Co., Inc. filed Critical Ajinomoto Co., Inc.
Priority to EP20080711306 priority Critical patent/EP2123756B1/en
Priority to KR1020097019138A priority patent/KR101162049B1/ko
Priority to CN2008800052009A priority patent/CN101631860B/zh
Priority to AU2008215422A priority patent/AU2008215422B2/en
Priority to JP2008558137A priority patent/JPWO2008099898A1/ja
Priority to NZ579599A priority patent/NZ579599A/en
Publication of WO2008099898A1 publication Critical patent/WO2008099898A1/ja
Priority to US12/541,220 priority patent/US9062291B2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/104—Aminoacyltransferases (2.3.2)
    • C12N9/1044—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/02—Aminoacyltransferases (2.3.2)
    • C12Y203/02013—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

　ＷＴトランスグルタミナーゼに、ジスルフィド結合（SS結合）を形成し得るシステイン残基への変異を導入することによって得られる、耐熱性及び／又はｐＨ安定性が向上したトランスグルタミナーゼ変異体タンパク質。
PCT/JP2008/052470 2007-02-15 2008-02-14 ジスルフィド結合導入トランスグルタミナーゼ WO2008099898A1 (ja)

Priority Applications (7)

Application Number Priority Date Filing Date Title
EP20080711306 EP2123756B1 (en) 2007-02-15 2008-02-14 Transglutaminase having disulfide bond introduced therein
KR1020097019138A KR101162049B1 (ko) 2007-02-15 2008-02-14 디설파이드 결합 도입 트랜스글루타미나제
CN2008800052009A CN101631860B (zh) 2007-02-15 2008-02-14 引入二硫键的转谷氨酰胺酶
AU2008215422A AU2008215422B2 (en) 2007-02-15 2008-02-14 Transglutaminase having disulfide bond introduced therein
JP2008558137A JPWO2008099898A1 (ja) 2007-02-15 2008-02-14 ジスルフィド結合導入トランスグルタミナーゼ
NZ579599A NZ579599A (en) 2007-02-15 2008-02-14 Transglutaminase having disulfide bond introduced therein
US12/541,220 US9062291B2 (en) 2007-02-15 2009-08-14 Transglutaminase having disulfide bond introduced therein

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007034916 2007-02-15
JP2007-034916 2007-02-15

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/541,220 Continuation US9062291B2 (en) 2007-02-15 2009-08-14 Transglutaminase having disulfide bond introduced therein

Publications (1)

Publication Number Publication Date
WO2008099898A1 true WO2008099898A1 (ja) 2008-08-21

Family

ID=39690125

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/052470 WO2008099898A1 (ja) 2007-02-15 2008-02-14 ジスルフィド結合導入トランスグルタミナーゼ

Country Status (8)

Country Link
US (1) US9062291B2 (ja)
EP (1) EP2123756B1 (ja)
JP (1) JPWO2008099898A1 (ja)
KR (1) KR101162049B1 (ja)
CN (1) CN101631860B (ja)
AU (1) AU2008215422B2 (ja)
NZ (1) NZ579599A (ja)
WO (1) WO2008099898A1 (ja)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009066785A1 (ja) 2007-11-19 2009-05-28 Ajinomoto Co., Inc. 繊維加工物及びその製造法
WO2010101256A1 (ja) 2009-03-06 2010-09-10 味の素株式会社 放線菌由来の耐熱性トランスグルタミナーゼ
JP2017519045A (ja) * 2014-06-12 2017-07-13 シー・エス・ピー・シー ドフェン コーポレーションＣｓｐｃ Ｄｏｐｈｅｎ Ｃｏｒｐｏｒａｔｉｏｎ 酵素的方法による均一抗体薬物コンジュゲート
CN112980759A (zh) * 2021-03-08 2021-06-18 上海交通大学 增强Subtilisin基因转录水平以提高TG酶发酵水平的方法
WO2021177392A1 (ja) 2020-03-04 2021-09-10 味の素株式会社 変異型トランスグルタミナーゼ
WO2022071061A1 (ja) 2020-09-29 2022-04-07 味の素株式会社 変異型トランスグルタミナーゼ

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2239335B1 (en) * 2008-01-18 2013-02-27 Ajinomoto Co., Inc. Process for production of serine derivative, and protein for use in the process
EP2302066A4 (en) * 2008-07-09 2012-02-08 Ajinomoto Kk METHOD FOR PRODUCING AMINOHYDROXYBENZOIC ACID
DE102010062600A1 (de) 2010-12-08 2012-06-14 Evonik Goldschmidt Gmbh Hydrophobisiertes Proteinhydrolysat
CN103608463A (zh) 2011-06-23 2014-02-26 味之素株式会社 由使具有免疫球蛋白折叠结构的蛋白和能够形成亚单元结构的蛋白融合而得到的单体蛋白组成的多聚体蛋白的制备方法
CN104379742A (zh) 2012-05-29 2015-02-25 味之素株式会社 生产3-乙酰氨基-4-羟基苯甲酸的方法
CA3177215A1 (en) 2017-09-19 2019-03-28 Daiichi Sankyo Company, Limited Antibody-pyrrolobenzodiazepine derivative conjugate
EP3719131A4 (en) * 2017-11-30 2021-09-01 Amano Enzyme Inc. MODIFIED TRANSGLUTAMINASE
CA3128571A1 (en) 2019-03-19 2020-09-24 Paul Scherrer Institut Transglutaminase conjugation method with a glycine based linker
WO2022058594A1 (en) 2020-09-18 2022-03-24 Araris Biotech Ag Transglutaminase conjugation method with amino acid-based linkers
KR20230096052A (ko) 2020-10-25 2023-06-29 아라리스 바이오테크 아게 항체-링커 접합체를 생성하기 위한 수단 및 방법
WO2023072934A1 (en) 2021-10-25 2023-05-04 Araris Biotech Ag Methods for producing antibody-linker conjugates
WO2023161291A1 (en) 2022-02-22 2023-08-31 Araris Biotech Ag Peptide linkers comprising two or more payloads

Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6427471A (en) 1987-03-04 1989-01-30 Ajinomoto Kk Novel enzyme and production of protein gelatinized product using said enzyme
JPH03213574A (ja) 1990-01-10 1991-09-18 Ajinomoto Co Inc 獣毛繊維の改質方法
EP0481504A1 (en) 1990-10-19 1992-04-22 Amano Pharmaceutical Co., Ltd. Recombinant transglutaminase
JPH05199883A (ja) 1990-10-19 1993-08-10 Amano Pharmaceut Co Ltd リコンビナントトランスグルタミナーゼ
JPH0630771A (ja) 1992-07-14 1994-02-08 Ajinomoto Co Inc バクテリアルトランスグルタミナーゼの製造法及び関連物
JPH10504721A (ja) 1994-08-26 1998-05-12 ノボ ノルディスク アクティーゼルスカブ 微生物のトランスグルタミナーゼ、それらの産生及び使用
WO2001023591A1 (fr) 1999-09-30 2001-04-05 Ajinomoto Co., Inc. Procédé de production de transglutaminase
JP2001321192A (ja) 2000-05-15 2001-11-20 Ajinomoto Co Inc 構造解析用タンパク質試料の調製方法
JP2002253272A (ja) * 2000-08-17 2002-09-10 Ajinomoto Co Inc 微生物由来トランスグルタミナーゼの改変方法
WO2002081694A1 (fr) 2001-03-30 2002-10-17 Ajinomoto Co.,Inc. Procede de secretion et de production de proteine
US6849095B2 (en) 2000-09-01 2005-02-01 Trumpler Gmbh & Co. Chemische Fabrik Agent and method for enzymatically tanning skins
WO2005103278A1 (ja) 2004-04-20 2005-11-03 Ajinomoto Co., Inc. タンパク質の製造法
JP2007034916A (ja) 2005-07-29 2007-02-08 Toshiba Tec Corp 商品販売登録データ処理装置

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
EP1310560A4 (en) * 2000-08-17 2005-07-13 Ajinomoto Kk METHOD FOR MODIFICATION OF TRANSGLUTAMINASE OF MICROORGANISMS (MTG)
CA2518049C (en) 2003-03-07 2013-05-07 Ajinomoto Co., Inc. Method of producing microbial transglutaminase
BRPI0412535A (pt) 2003-07-16 2006-09-19 Ajinomoto Kk serina acetiltransferase mutante, dna, bactéria, e, método para produzir l-cisteìna

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6427471A (en) 1987-03-04 1989-01-30 Ajinomoto Kk Novel enzyme and production of protein gelatinized product using said enzyme
JPH03213574A (ja) 1990-01-10 1991-09-18 Ajinomoto Co Inc 獣毛繊維の改質方法
EP0481504A1 (en) 1990-10-19 1992-04-22 Amano Pharmaceutical Co., Ltd. Recombinant transglutaminase
JPH05199883A (ja) 1990-10-19 1993-08-10 Amano Pharmaceut Co Ltd リコンビナントトランスグルタミナーゼ
JPH0630771A (ja) 1992-07-14 1994-02-08 Ajinomoto Co Inc バクテリアルトランスグルタミナーゼの製造法及び関連物
JPH10504721A (ja) 1994-08-26 1998-05-12 ノボ ノルディスク アクティーゼルスカブ 微生物のトランスグルタミナーゼ、それらの産生及び使用
WO2001023591A1 (fr) 1999-09-30 2001-04-05 Ajinomoto Co., Inc. Procédé de production de transglutaminase
JP2001321192A (ja) 2000-05-15 2001-11-20 Ajinomoto Co Inc 構造解析用タンパク質試料の調製方法
JP2002253272A (ja) * 2000-08-17 2002-09-10 Ajinomoto Co Inc 微生物由来トランスグルタミナーゼの改変方法
US6849095B2 (en) 2000-09-01 2005-02-01 Trumpler Gmbh & Co. Chemische Fabrik Agent and method for enzymatically tanning skins
WO2002081694A1 (fr) 2001-03-30 2002-10-17 Ajinomoto Co.,Inc. Procede de secretion et de production de proteine
WO2005103278A1 (ja) 2004-04-20 2005-11-03 Ajinomoto Co., Inc. タンパク質の製造法
JP2007034916A (ja) 2005-07-29 2007-02-08 Toshiba Tec Corp 商品販売登録データ処理装置

Non-Patent Citations (21)

* Cited by examiner, † Cited by third party
Title
A. ICHINOSE ET AL., BIOCHEMISTRY, vol. 25, 1986, pages 6900
ALTSCHUL ET AL., NUCLEIC ACIDS RES., vol. 25, 1997, pages 3389 - 3402
APP. ENV. MICRO., vol. 69, 2003, pages 3011 - 3014
CLARKE J. ET AL.: "Engineered disulfide bonds as probes of the bonding pathway of barnase: Increasing the stability of proteins against the rate of denaturation", BIOCHEMISTRY, vol. 32, 1993, pages 4322 - 4329, XP008116664 *
FEMS MICROBIOL. LETT., vol. 53, 1989, pages 299 - 303
J. BIOL. CHEM. 25, vol. 273, no. 52, 1998, pages 34868 - 74
J. BIOL. CHEM., vol. 268, no. 16, 1993, pages 11565 - 11572
J.BIOL.CHEM., vol. 241, 1966, pages 5518 - 5525
K. IKURA ET AL., BIOCHEMISTRY, vol. 27, 1988, pages 2898
KARLIN ET AL., PROC. NATL. ACAD. SCI., vol. 90, 1993, pages 5873 - 5877
KASHIWAGI T. ET AL.: "Crystal structure of microbial transglutaminase from Streptoverticillium mobaraense", J. BIOL. CHEM., vol. 277, no. 46, 2002, pages 44252 - 44260, XP002405972 *
LI Y. ET AL.: "Effect on thermostability and catalytic activity of introducing disulfide bonds nto Aspergillus awamori glucoamylase", PROTEIN ENG., vol. 11, no. 8, 1998, pages 661 - 667, XP008116663 *
M.A. PHILLIPS ET AL., PROC. NATL. ACAD. SCI., vol. 87, 1990, pages 9333
MATSUMURA M. ET AL.: "Substantial increase of protein stability by multiple disulphide bonds", NATURE, vol. 342, no. 6247, 1989, pages 291 - 293, XP002973649 *
MYERS; MILLER, CABIOS, vol. 4, 1988, pages 11 - 17
NEEDLEMAN ET AL., J. MOL. BIOL., vol. 48, 1970, pages 444 - 453
PEARSON ET AL., PROC. NATL. ACAD. SCI., vol. 85, 1988, pages 2444 - 2448
PERRY L.J. ET AL.: "Disulfide bond engineered into T4 lysozyme: Stabilization of the protein toward thermal inactivation", SCIENCE, vol. 226, no. 4674, 1984, pages 555 - 557, XP008116665 *
PROT. EXP. PURI., vol. 26, 2002, pages 329 - 335
See also references of EP2123756A4 *
T. KANAJI ET AL., JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 268, 1993, pages 11565

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009066785A1 (ja) 2007-11-19 2009-05-28 Ajinomoto Co., Inc. 繊維加工物及びその製造法
WO2010101256A1 (ja) 2009-03-06 2010-09-10 味の素株式会社 放線菌由来の耐熱性トランスグルタミナーゼ
CN102341497A (zh) * 2009-03-06 2012-02-01 味之素株式会社 放线菌来源的耐热性转谷氨酰胺酶
US8580537B2 (en) 2009-03-06 2013-11-12 Ajinomoto Co., Inc. Thermotolerant transglutaminase originating in actinomyces
JP2017519045A (ja) * 2014-06-12 2017-07-13 シー・エス・ピー・シー ドフェン コーポレーションＣｓｐｃ Ｄｏｐｈｅｎ Ｃｏｒｐｏｒａｔｉｏｎ 酵素的方法による均一抗体薬物コンジュゲート
WO2021177392A1 (ja) 2020-03-04 2021-09-10 味の素株式会社 変異型トランスグルタミナーゼ
WO2022071061A1 (ja) 2020-09-29 2022-04-07 味の素株式会社 変異型トランスグルタミナーゼ
CN112980759A (zh) * 2021-03-08 2021-06-18 上海交通大学 增强Subtilisin基因转录水平以提高TG酶发酵水平的方法

Also Published As

Publication number Publication date
JPWO2008099898A1 (ja) 2010-05-27
EP2123756A4 (en) 2010-06-30
EP2123756A1 (en) 2009-11-25
CN101631860B (zh) 2012-07-18
AU2008215422B2 (en) 2011-07-14
KR101162049B1 (ko) 2012-07-04
CN101631860A (zh) 2010-01-20
EP2123756B1 (en) 2014-06-04
US9062291B2 (en) 2015-06-23
US20100143970A1 (en) 2010-06-10
KR20090120485A (ko) 2009-11-24
NZ579599A (en) 2012-03-30
AU2008215422A1 (en) 2008-08-21

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