WO2008084571A1 - 核酸応答性ゲルおよびその製造方法ならびにその利用 - Google Patents

核酸応答性ゲルおよびその製造方法ならびにその利用 Download PDF

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Publication number
WO2008084571A1
WO2008084571A1 PCT/JP2007/065384 JP2007065384W WO2008084571A1 WO 2008084571 A1 WO2008084571 A1 WO 2008084571A1 JP 2007065384 W JP2007065384 W JP 2007065384W WO 2008084571 A1 WO2008084571 A1 WO 2008084571A1
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WO
WIPO (PCT)
Prior art keywords
gel
acid
nucleic
reactive
nucleic acid
Prior art date
Application number
PCT/JP2007/065384
Other languages
English (en)
French (fr)
Inventor
Takashi Miyata
Tadashi Uragami
Kaori Okawa
Original Assignee
Japan Science And Technology Agency
A School Corporation Kansai University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP2007005227A external-priority patent/JP5234887B2/ja
Application filed by Japan Science And Technology Agency, A School Corporation Kansai University filed Critical Japan Science And Technology Agency
Priority to US12/448,857 priority Critical patent/US8937171B2/en
Publication of WO2008084571A1 publication Critical patent/WO2008084571A1/ja

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

　構造設計によって体積変化の向上が可能であるとともに、核酸の認識能を調節することができ、感度が向上し、標的ＤＮＡの配列などに応じてデザインしやすい核酸応答性ゲルを提供する。ハイブリダイズしている２本の一本鎖核酸からなるプローブが、高分子ゲルの網目構造内に固定されている核酸応答性ゲルであって、当該プローブは、２本の一本鎖核酸が可逆的に結合している。
PCT/JP2007/065384 2007-01-12 2007-08-06 核酸応答性ゲルおよびその製造方法ならびにその利用 WO2008084571A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US12/448,857 US8937171B2 (en) 2007-01-12 2007-08-06 Nucleic-acid-responsive gel, method for producing same, and use of same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007005227A JP5234887B2 (ja) 2006-01-13 2007-01-12 核酸応答性ゲルおよびその製造方法ならびにその利用
JP2007-005227 2007-01-12

Publications (1)

Publication Number Publication Date
WO2008084571A1 true WO2008084571A1 (ja) 2008-07-17

Family

ID=39608471

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2007/065384 WO2008084571A1 (ja) 2007-01-12 2007-08-06 核酸応答性ゲルおよびその製造方法ならびにその利用

Country Status (2)

Country Link
US (1) US8937171B2 (ja)
WO (1) WO2008084571A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
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WO2012117588A1 (ja) * 2011-02-28 2012-09-07 独立行政法人科学技術振興機構 複数物質応答性ゲルおよびその製造方法並びにその利用

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US20090118218A1 (en) * 2006-07-11 2009-05-07 Joshua Stopek Biocompatible Hydrogels
US9372187B2 (en) * 2007-12-28 2016-06-21 Ortho-Clinical Diagnostics Kabushiki Kaisha Detection method and determination method for detection target
CN106977731B (zh) * 2017-01-23 2019-11-15 武汉大学 一种偶联核酸或蛋白质的温敏聚合物及其应用
CN113083172B (zh) * 2021-04-13 2022-04-19 清华大学 一种力学性能提高的核酸水凝胶及其制备方法和用途

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WO2002090990A1 (en) * 2001-05-09 2002-11-14 Kyowa Medex Co., Ltd. Biopolymer-containing gel
JP2005106533A (ja) * 2003-09-29 2005-04-21 Institute Of Physical & Chemical Research 核酸応答性ゲル
JP2006138656A (ja) * 2004-11-10 2006-06-01 Kyowa Medex Co Ltd 分子インプリントゲル、その製造方法、該分子インプリントゲルを用いる検体中の対象分子の測定方法および対象分子測定用試薬

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JP2006137805A (ja) 2004-11-10 2006-06-01 Tohoku Univ 対象化合物を捕捉することができるモレキュラーインプリンテッドポリマー、その調製方法、およびモレキュラーインプリンテッドポリマーにより対象試料中の対象化合物を捕捉する方法
JP5386702B2 (ja) 2005-02-21 2014-01-15 国立大学法人 東京大学 ポリロタキサン及びポリマーを有する材料、及びその製造方法
JP2006257139A (ja) 2005-03-15 2006-09-28 Osaka Prefecture コア−シェル型高分子微粒子及びその製造方法
JP5120808B2 (ja) 2005-04-28 2013-01-16 独立行政法人科学技術振興機構 センサー素子およびこれを用いた外部刺激測定装置並びに外部刺激の測定方法
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002090990A1 (en) * 2001-05-09 2002-11-14 Kyowa Medex Co., Ltd. Biopolymer-containing gel
JP2005106533A (ja) * 2003-09-29 2005-04-21 Institute Of Physical & Chemical Research 核酸応答性ゲル
JP2006138656A (ja) * 2004-11-10 2006-06-01 Kyowa Medex Co Ltd 分子インプリントゲル、その製造方法、該分子インプリントゲルを用いる検体中の対象分子の測定方法および対象分子測定用試薬

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MIYATA T. ET AL.: "Nihonsa DNA o Kakyoten to suru DNA Otosei Gel no Gosei to Sono Ninshiki Oto Kyodo", POLYMER PREPRINTS, JAPAN, vol. 55, no. 1, 10 May 2006 (2006-05-10), pages 2J19, XP003022930 *
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012117588A1 (ja) * 2011-02-28 2012-09-07 独立行政法人科学技術振興機構 複数物質応答性ゲルおよびその製造方法並びにその利用
JP5093633B2 (ja) * 2011-02-28 2012-12-12 独立行政法人科学技術振興機構 複数物質応答性ゲルおよびその製造方法並びにその利用
US8846412B2 (en) 2011-02-28 2014-09-30 Japan Science And Technology Agency Multiple substances-responsive gel, method for producing same, and utilization of same

Also Published As

Publication number Publication date
US8937171B2 (en) 2015-01-20
US20100081204A1 (en) 2010-04-01

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