WO2010036359A3 - Methods of polymerase activity assay - Google Patents

Methods of polymerase activity assay Download PDF

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Publication number
WO2010036359A3
WO2010036359A3 PCT/US2009/005326 US2009005326W WO2010036359A3 WO 2010036359 A3 WO2010036359 A3 WO 2010036359A3 US 2009005326 W US2009005326 W US 2009005326W WO 2010036359 A3 WO2010036359 A3 WO 2010036359A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
activity assay
polymerase activity
disclosed
polymerase
Prior art date
Application number
PCT/US2009/005326
Other languages
French (fr)
Other versions
WO2010036359A2 (en
Inventor
Xing Xin
Fei Mao
Original Assignee
Allelogic Biosciences Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Allelogic Biosciences Corporation filed Critical Allelogic Biosciences Corporation
Publication of WO2010036359A2 publication Critical patent/WO2010036359A2/en
Publication of WO2010036359A3 publication Critical patent/WO2010036359A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/91245—Nucleotidyltransferases (2.7.7)
    • G01N2333/9125—Nucleotidyltransferases (2.7.7) with a definite EC number (2.7.7.-)
    • G01N2333/9126—DNA-directed DNA polymerase (2.7.7.7)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Abstract

Compositions, methods, and kits for determining the activity of a nucleic acid polymerase are disclosed where fluorescent double stranded DNA binding dye and oligonucleotides are employed. Also disclosed are method for detecting inhibitory activity of a target molecule to a polymerase.
PCT/US2009/005326 2008-09-25 2009-09-25 Methods of polymerase activity assay WO2010036359A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19440508P 2008-09-25 2008-09-25
US61/194,405 2008-09-25

Publications (2)

Publication Number Publication Date
WO2010036359A2 WO2010036359A2 (en) 2010-04-01
WO2010036359A3 true WO2010036359A3 (en) 2010-06-17

Family

ID=42060338

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/005326 WO2010036359A2 (en) 2008-09-25 2009-09-25 Methods of polymerase activity assay

Country Status (1)

Country Link
WO (1) WO2010036359A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011142719A1 (en) * 2010-05-14 2011-11-17 Staalhandske Per Kit and method
WO2012024639A1 (en) * 2010-08-20 2012-02-23 Life Technologies Corporation Polymerase assay with a fret substrate
CA3190291A1 (en) 2014-08-11 2016-02-18 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
EP3098322A1 (en) * 2015-05-29 2016-11-30 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method to detect activity of a polymerase
CN109652501B (en) * 2017-10-12 2022-06-07 深圳华大智造科技股份有限公司 Method and kit for detecting 3'-5' exoactivity of nuclease to specific base
CN112746094B (en) * 2019-10-29 2023-06-13 深圳清华大学研究院 Method for measuring kinetic parameters of pre-steady-state enzymatic reaction of DNA polymerase
CN110904187A (en) * 2019-11-05 2020-03-24 翌圣生物科技（上海）有限公司 Taq enzyme activity determination method
KR102441122B1 (en) * 2020-05-11 2022-09-07 주식회사 엔지노믹스 Method for measuring polymerase activity
US20220136021A1 (en) 2020-10-30 2022-05-05 Microsoft Technology Licensing, Llc Spatially addressable control of polymerase activity

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050032076A1 (en) * 1998-05-01 2005-02-10 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and dna molecules
US20050064432A1 (en) * 2003-09-19 2005-03-24 Linden Technologies, Inc. Nucleic acid amplification and detection
US20070059701A1 (en) * 2003-06-30 2007-03-15 Astrazeneca Ab Fluorescence assays for nucleic acid polymerase activity
WO2007038422A2 (en) * 2005-09-26 2007-04-05 Allelogic Biosciences Corp. Oligonucleotides as temperature-sensitive inhibitors for dna polymerases

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050032076A1 (en) * 1998-05-01 2005-02-10 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and dna molecules
US20070059701A1 (en) * 2003-06-30 2007-03-15 Astrazeneca Ab Fluorescence assays for nucleic acid polymerase activity
US20050064432A1 (en) * 2003-09-19 2005-03-24 Linden Technologies, Inc. Nucleic acid amplification and detection
WO2007038422A2 (en) * 2005-09-26 2007-04-05 Allelogic Biosciences Corp. Oligonucleotides as temperature-sensitive inhibitors for dna polymerases

Also Published As

Publication number Publication date
WO2010036359A2 (en) 2010-04-01

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