WO2008079169A2 - Nanonozzle device arrays: their preparation and use for macromolecular analysis - Google Patents

Nanonozzle device arrays: their preparation and use for macromolecular analysis Download PDF

Info

Publication number
WO2008079169A2
WO2008079169A2 PCT/US2007/016408 US2007016408W WO2008079169A2 WO 2008079169 A2 WO2008079169 A2 WO 2008079169A2 US 2007016408 W US2007016408 W US 2007016408W WO 2008079169 A2 WO2008079169 A2 WO 2008079169A2
Authority
WO
WIPO (PCT)
Prior art keywords
nanochannel
macromolecule
gradient
detection system
constriction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2007/016408
Other languages
English (en)
French (fr)
Other versions
WO2008079169A3 (en
Inventor
Han Cao
Parikshit A. Deshpande
Michael D. Austin
Michael Boyce-Jacino
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bionano Genomics Inc
Original Assignee
Bionanomatrix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to AU2007338862A priority Critical patent/AU2007338862B2/en
Priority to US12/374,141 priority patent/US9061901B2/en
Priority to CA2658122A priority patent/CA2658122C/en
Priority to EP07872156A priority patent/EP2049262A2/en
Priority to JP2009520847A priority patent/JP2010510476A/ja
Priority to EP21192850.2A priority patent/EP3978121A1/en
Application filed by Bionanomatrix Inc filed Critical Bionanomatrix Inc
Publication of WO2008079169A2 publication Critical patent/WO2008079169A2/en
Publication of WO2008079169A3 publication Critical patent/WO2008079169A3/en
Anticipated expiration legal-status Critical
Priority to US14/712,816 priority patent/US9845238B2/en
Priority to US15/801,081 priority patent/US10676352B2/en
Priority to US16/889,256 priority patent/US11529630B2/en
Priority to US18/056,074 priority patent/US20230126737A1/en
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0663—Stretching or orienting elongated molecules or particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0822—Slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0896—Nanoscaled
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid
    • G01N2035/1039—Micropipettes, e.g. microcapillary tubes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Definitions

  • FIG. 6a depicts the preparation of a constriction at the end of a nanochannel by additive material deposition
  • FIG 6b is a scanning electron micrograph of an embodiment of such a constriction at the end of a nanochannel
  • FIG. 7 depicts a representative fabrication of a constriction at the end of a nanochannel by (FIG. 7a) deposition of material at the end of the nanochannel resulting in complete sealing of the channel; (FIG. 7b) self-terminating opening of the constriction using an acid etchant terminated by exposure to a neutralizing strong base; and (FIG. 7c) the final nanonozzle device after the etchant and neutralizer are removed;
  • the present invention provides methods for characterizing one or more features of a macromolecule. These methods include linearizing a macromolecule residing at least in part within a nanochannel, at least a portion of the nanochannel being capable of physically constraining at least a portion of the macromolecule so as to maintain in linear form that portion of the macromolecule.
  • Example 1 A silicon substrate was provided having a plurality of parallel linear channels having a 150 nm trench width and a 150 nm trench height. These channel openings are sputtered at a gas pressure of 5 mTorr according to the general procedures given above. The sputter deposition time was 10-25 minutes to provide a nanochannel array that can either be partially sealed or completely sealed.
  • Example 5 This example describes filling a nanofluidic chip with a fluid containing DNA macromolecules to analyze DNA.
  • a cylindrical-shaped plastic sample-delivery tube of 2 mm diameter was placed in fluid communication with one of the reservoirs of the nanochannel array of Example 3.
  • the delivery tube can be connected to an external sample delivery/collection device, which can be in turn connected to a pressure /vaccum generating apparatus.
  • the nanochannels are wetted using capillary action with a buffer solution.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Nanotechnology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Clinical Laboratory Science (AREA)
  • Fluid Mechanics (AREA)
  • Composite Materials (AREA)
  • Dispersion Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Materials Engineering (AREA)
  • Electrochemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
PCT/US2007/016408 2006-07-19 2007-07-19 Nanonozzle device arrays: their preparation and use for macromolecular analysis Ceased WO2008079169A2 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
EP21192850.2A EP3978121A1 (en) 2006-07-19 2007-07-19 Nanonozzle device arrays: their preparation and use for macromolecular analysis
US12/374,141 US9061901B2 (en) 2006-07-19 2007-07-19 Nanonozzle device arrays: their preparation and use for macromolecular analysis
CA2658122A CA2658122C (en) 2006-07-19 2007-07-19 Nanonozzle device arrays: their preparation and use for macromolecular analysis
EP07872156A EP2049262A2 (en) 2006-07-19 2007-07-19 Nanonozzle device arrays: their preparation and use for macromolecular analysis
JP2009520847A JP2010510476A (ja) 2006-07-19 2007-07-19 ナノノズル装置アレイ：その作製および高分子分析における利用
AU2007338862A AU2007338862B2 (en) 2006-07-19 2007-07-19 Nanonozzle device arrays: their preparation and use for macromolecular analysis
US14/712,816 US9845238B2 (en) 2006-07-19 2015-05-14 Nanonozzle device arrays: their preparation and use for macromolecular analysis
US15/801,081 US10676352B2 (en) 2006-07-19 2017-11-01 Nanonozzle device arrays: their preparation and use for macromolecular analysis
US16/889,256 US11529630B2 (en) 2006-07-19 2020-06-01 Nanonozzle device arrays: their preparation and use for macromolecular analysis
US18/056,074 US20230126737A1 (en) 2006-07-19 2022-11-16 Nanonozzle device arrays: their preparation and use for macromolecular analysis

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US83177206P 2006-07-19 2006-07-19
US60/831,772 2006-07-19
US90858407P 2007-03-28 2007-03-28
US90858207P 2007-03-28 2007-03-28
US60/908,582 2007-03-28
US60/908,584 2007-03-28

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/374,141 A-371-Of-International US9061901B2 (en) 2006-07-19 2007-07-19 Nanonozzle device arrays: their preparation and use for macromolecular analysis
US14/712,816 Division US9845238B2 (en) 2006-07-19 2015-05-14 Nanonozzle device arrays: their preparation and use for macromolecular analysis

Publications (2)

Publication Number Publication Date
WO2008079169A2 true WO2008079169A2 (en) 2008-07-03
WO2008079169A3 WO2008079169A3 (en) 2008-10-02

Family

ID=39563064

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/016408 Ceased WO2008079169A2 (en) 2006-07-19 2007-07-19 Nanonozzle device arrays: their preparation and use for macromolecular analysis

Country Status (8)

Country Link
US (5) US9061901B2 (enExample)
EP (2) EP3978121A1 (enExample)
JP (2) JP2010510476A (enExample)
CN (1) CN103203256B (enExample)
AU (1) AU2007338862B2 (enExample)
CA (1) CA2658122C (enExample)
SG (1) SG173398A1 (enExample)
WO (1) WO2008079169A2 (enExample)

Cited By (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010042007A1 (en) * 2008-10-10 2010-04-15 Jonas Tegenfeldt Method for the mapping of the local at/gc ratio along dna
WO2011067961A1 (ja) * 2009-12-02 2011-06-09 独立行政法人科学技術振興機構 流路デバイス及びそれを含むサンプル処理装置
US20120040343A1 (en) * 2008-12-19 2012-02-16 Gregory Timp Detecting and Sorting Methylated DNA Using a Synthetic Nanopore
WO2013119765A1 (en) 2012-02-10 2013-08-15 The University Of North Carolina At Chapel Hill Devices with fluidic nanofunnels, associated methods, fabrication and analysis systems
US8748091B2 (en) 2009-12-18 2014-06-10 The Board Of Trustees Of The University Of Illinois Characterizing stretched polynucleotides in a synthetic nanopassage
US8871921B2 (en) 2008-09-03 2014-10-28 Quantumdx Group Ltd. Design, synthesis and use of synthetic nucleotides comprising charge mass tags
US8882980B2 (en) 2008-09-03 2014-11-11 Nabsys, Inc. Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US8926813B2 (en) 2008-09-03 2015-01-06 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
US9051609B2 (en) 2007-10-01 2015-06-09 Nabsys, Inc. Biopolymer Sequencing By Hybridization of probes to form ternary complexes and variable range alignment
WO2015170782A1 (en) * 2014-05-08 2015-11-12 Osaka University Devices, systems and methods for linearization of polymers
US9255288B2 (en) 2013-03-13 2016-02-09 The University Of North Carolina At Chapel Hill Nanofluidic devices for the rapid mapping of whole genomes and related systems and methods of analysis
US9410196B2 (en) 2008-09-03 2016-08-09 Quantumdx Group Limited Methods and kits for nucleic acid sequencing
US9434981B2 (en) 2010-09-27 2016-09-06 Nabsys 2.0 Llc Assay methods using nicking endonucleases
US9535033B2 (en) 2012-08-17 2017-01-03 Quantum Biosystems Inc. Sample analysis method
EP2668645A4 (en) * 2011-01-24 2017-01-04 Genia Technologies, Inc. System for communicating information from an array of sensors
WO2017009710A3 (en) * 2015-07-16 2017-02-23 The Hong Kong University Of Science And Technology Dynamic formation of nanochannels for single-molecule dna analysis
US9605307B2 (en) 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US9617595B2 (en) 2010-02-08 2017-04-11 Genia Technologies, Inc. Systems and methods for assembling a lipid bilayer on a substantially planar solid surface
US9644236B2 (en) 2013-09-18 2017-05-09 Quantum Biosystems Inc. Biomolecule sequencing devices, systems and methods
US9650668B2 (en) 2008-09-03 2017-05-16 Nabsys 2.0 Llc Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US9678055B2 (en) 2010-02-08 2017-06-13 Genia Technologies, Inc. Methods for forming a nanopore in a lipid bilayer
US9702003B2 (en) 2010-11-16 2017-07-11 Nabsys 2.0 Llc Methods for sequencing a biomolecule by detecting relative positions of hybridized probes
US9759711B2 (en) 2013-02-05 2017-09-12 Genia Technologies, Inc. Nanopore arrays
US9809855B2 (en) 2013-02-20 2017-11-07 Bionano Genomics, Inc. Characterization of molecules in nanofluidics
US9914966B1 (en) 2012-12-20 2018-03-13 Nabsys 2.0 Llc Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation
US10036725B2 (en) 2013-10-17 2018-07-31 Genia Technologies, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
US10202644B2 (en) 2010-03-03 2019-02-12 Quantum Biosystems Inc. Method and device for identifying nucleotide, and method and device for determining nucleotide sequence of polynucleotide
US10261066B2 (en) 2013-10-16 2019-04-16 Quantum Biosystems Inc. Nano-gap electrode pair and method of manufacturing same
US10261065B2 (en) 2013-02-28 2019-04-16 The University Of North Carolina At Chapel Hill Nanofluidic devices with integrated components for the controlled capture, trapping, and transport of macromolecules and related methods of analysis
US10294516B2 (en) 2013-01-18 2019-05-21 Nabsys 2.0 Llc Enhanced probe binding
US10438811B1 (en) 2014-04-15 2019-10-08 Quantum Biosystems Inc. Methods for forming nano-gap electrodes for use in nanosensors
US10471428B2 (en) 2015-05-11 2019-11-12 The University Of North Carolina At Chapel Hill Fluidic devices with nanoscale manifolds for molecular transport, related systems and methods of analysis
US10724987B2 (en) 2012-02-27 2020-07-28 Roche Sequencing Solutions, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
US10759824B2 (en) 2008-09-03 2020-09-01 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags
US10844424B2 (en) 2013-02-20 2020-11-24 Bionano Genomics, Inc. Reduction of bias in genomic coverage measurements
US11053535B2 (en) 2011-09-12 2021-07-06 The University Of North Carolina At Chapel Hill Devices with a fluid transport nanochannel intersected by a fluid sensing nanochannel and related methods
US11180523B2 (en) 2016-01-04 2021-11-23 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags
US11274341B2 (en) 2011-02-11 2022-03-15 NABsys, 2.0 LLC Assay methods using DNA binding proteins
EP3984640A1 (en) * 2020-10-16 2022-04-20 Universität Hamburg An autonomous nanofluidic analysis device and a method for the analysis of dna molecules
US11773429B2 (en) 2014-02-25 2023-10-03 Bionano Genomics, Inc. Reduction of bias in genomic coverage measurements
US12091712B2 (en) 2016-04-27 2024-09-17 Illumina Cambridge, Ltd. Systems and methods for measurement and sequencing of bio-molecules

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3978121A1 (en) 2006-07-19 2022-04-06 BioNano Genomics, Inc. Nanonozzle device arrays: their preparation and use for macromolecular analysis
US8722327B2 (en) 2007-03-28 2014-05-13 Bionano Genomics, Inc. Methods of macromolecular analysis using nanochannel arrays
WO2009046445A1 (en) * 2007-10-04 2009-04-09 Halcyon Molecular Sequencing nucleic acid polymers with electron microscopy
US8628919B2 (en) 2008-06-30 2014-01-14 Bionano Genomics, Inc. Methods and devices for single-molecule whole genome analysis
JP5846703B2 (ja) 2008-11-18 2016-01-20 バイオナノ ジェノミックス、インク． ポリヌクレオチドのマッピング及び配列決定
US9422154B2 (en) 2010-11-02 2016-08-23 International Business Machines Corporation Feedback control of dimensions in nanopore and nanofluidic devices
KR101203292B1 (ko) 2011-05-13 2012-11-20 이상현 나노 미세 기공의 가공방법
JP2013042681A (ja) * 2011-08-23 2013-03-04 Kyocer Slc Technologies Corp 遺伝子解析用配線基板
US9718668B2 (en) 2012-02-16 2017-08-01 Board Of Trustees Of The University Of Arkansas Method of fabricating a nanochannel system for DNA sequencing and nanoparticle characterization
US8969827B2 (en) * 2012-07-09 2015-03-03 Materials Analysis Technology (Us) Corp. Specimen preparation for transmission electron microscopy
WO2014036265A2 (en) * 2012-08-30 2014-03-06 Atfi Transport of liquids and solute materials in nanochannels
US9791453B2 (en) 2012-12-26 2017-10-17 International Business Machines Corporation Methods for determining binding capability of target ligands with G protein-coupled receptors using translocation through nanochannels
US8835362B2 (en) 2012-12-26 2014-09-16 International Business Machines Corporation Modifying single proteins (GPCR), ligands, and nanopore surfaces to create binding-induced molecular changes of protein-ligand complexes detected in nanochannel translocation
CN105143462A (zh) 2013-02-05 2015-12-09 生物纳米基因有限公司 用于单分子分析的方法
US9687847B1 (en) * 2013-02-13 2017-06-27 University Of South Florida Microfluidic platforms for optical biosensing
US10139390B2 (en) * 2013-06-28 2018-11-27 Hitachi High-Technologies Corporation Analysis device
US20150037787A1 (en) * 2013-07-31 2015-02-05 International Business Machines Corporation Polynucleotide configuration for reliable electrical and optical sensing
RU142580U1 (ru) * 2013-08-02 2014-06-27 Байонано Дженомикс, Инк. Система наноанализа
JP6334118B2 (ja) * 2013-09-24 2018-05-30 クオンタムバイオシステムズ株式会社 単分子識別方法、装置、及びプログラム
US9303310B2 (en) 2013-10-15 2016-04-05 International Business Machines Corporation Nanofluidic sensor comprising spatially separated functional sensing components
US9255321B2 (en) 2013-10-15 2016-02-09 Globalfoundries Inc. Directed surface functionalization on selected surface areas of topographical features with nanometer resolution
US10024851B2 (en) 2013-10-15 2018-07-17 International Business Machines Corporation Use of disulfide bonds to form a reversible and reusable coating for nanofluidic devices
GB2534737B (en) * 2013-11-08 2020-08-05 Hitachi High Tech Corp DNA transport control device and method for producing same, as well as DNA sequencing device
CN106414767A (zh) * 2014-01-24 2017-02-15 量子生物有限公司 生物分子的测序装置、系统及方法
US9921181B2 (en) 2014-06-26 2018-03-20 International Business Machines Corporation Detection of translocation events using graphene-based nanopore assemblies
US9557294B2 (en) 2014-12-19 2017-01-31 Genia Technologies, Inc. Nanopore-based sequencing with varying voltage stimulus
US9863904B2 (en) 2014-12-19 2018-01-09 Genia Technologies, Inc. Nanopore-based sequencing with varying voltage stimulus
KR102292998B1 (ko) * 2015-01-16 2021-08-26 더 리전트 오브 더 유니버시티 오브 캘리포니아 핵산 증폭용 led 구동 플라즈몬 가열 장치
JP6433804B2 (ja) * 2015-02-09 2018-12-05 株式会社東芝 マイクロ分析パッケージ及びパッケージ基板
US10126262B2 (en) * 2015-09-24 2018-11-13 Genia Technologies, Inc. Differential output of analog memories storing nanopore measurement samples
WO2023114548A1 (en) * 2021-12-17 2023-06-22 Avery Digital Data, Inc. Electronic assembly of long dna molecules
US10974244B2 (en) * 2017-01-19 2021-04-13 The Board Of Trustees Of The University Of Alabama Branched nanochannel devices for detection and sorting of nucleic acids
JP6950954B2 (ja) * 2017-12-22 2021-10-13 国立大学法人金沢大学 液体充填方法、ｓｉｃｍ用プローブの製造方法、ｓｉｃｍ用プローブ及びｓｉｃｍ
US10649098B2 (en) 2018-01-29 2020-05-12 Samsung Electronics Co., Ltd. Light converting nanoparticle, method of making the light converting nanoparticle, and composition and optical film comprising the same
JP2018151397A (ja) * 2018-05-01 2018-09-27 クオンタムバイオシステムズ株式会社 単分子識別方法、装置、及びプログラム
DE102019200929A1 (de) * 2019-01-25 2020-07-30 Robert Bosch Gmbh Verfahren und Vorrichtung zum optischen Erfassen einer Länge eines Makromoleküls
CN111250182B (zh) * 2020-02-11 2021-03-19 北京理工大学 一种高通量微流控电泳筛分芯片及其制备方法、应用方法
US12012329B2 (en) 2020-02-13 2024-06-18 Board Of Trustees Of The University Of Arkansas Carbyne-based sensing device for high spatial resolution in DNA sequencing and biomolecule characterization and method of fabricating the same
US11837302B1 (en) 2020-08-07 2023-12-05 Iridia, Inc. Systems and methods for writing and reading data stored in a polymer using nano-channels
US11545213B1 (en) 2020-08-07 2023-01-03 Iridia, Inc. Systems and methods for writing and reading data stored in a polymer using nano-channels
JP7780783B2 (ja) * 2021-03-04 2025-12-05 国立大学法人大阪大学 デバイス、トンネル電流測定装置、核酸配列読取装置、トンネル電流測定方法、および、核酸配列読取方法
EP4562400A2 (en) * 2022-07-28 2025-06-04 Armonica Technologies, Inc. Polymer sequencing apparatus
EP4639178A1 (en) 2022-12-25 2025-10-29 Bionano Genomics, Inc. Optical genome mapping system
WO2024145268A2 (en) 2022-12-25 2024-07-04 Bionano Genomics, Inc. Optical genome mapping cartridges
EP4639177A1 (en) 2022-12-25 2025-10-29 Bionano Genomics, Inc. Instrumentation of optical genome mapping systems

Family Cites Families (98)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6147198A (en) 1988-09-15 2000-11-14 New York University Methods and compositions for the manipulation and characterization of individual nucleic acid molecules
US6150089A (en) 1988-09-15 2000-11-21 New York University Method and characterizing polymer molecules or the like
US5720928A (en) 1988-09-15 1998-02-24 New York University Image processing and analysis of individual nucleic acid molecules
JPH02176457A (ja) 1988-09-15 1990-07-09 Carnegie Inst Of Washington パルス志向電気泳動
EP0391674B1 (en) 1989-04-05 1996-03-20 New York University A method for characterising particles
US5079169A (en) 1990-05-22 1992-01-07 The Regents Of The Stanford Leland Junior University Method for optically manipulating polymer filaments
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
JP3123249B2 (ja) 1991-09-10 2001-01-09 株式会社日立製作所 Ｄｎａ分子の長さ計測方法および計測装置
US5314829A (en) 1992-12-18 1994-05-24 California Institute Of Technology Method for imaging informational biological molecules on a semiconductor substrate
US5427663A (en) * 1993-06-08 1995-06-27 British Technology Group Usa Inc. Microlithographic array for macromolecule and cell fractionation
US5637458A (en) 1994-07-20 1997-06-10 Sios, Inc. Apparatus and method for the detection and assay of organic molecules
GB9417211D0 (en) 1994-08-25 1994-10-12 Solicitor For The Affairs Of H Nucleotide sequencing method
US8142708B2 (en) 1995-04-03 2012-03-27 Wisconsin Alumni Research Foundation Micro fluidic system for single molecule imaging
US7775368B2 (en) * 1995-04-03 2010-08-17 Wisconsin Alumni Research Foundation Micro-channel long molecule manipulation system
EP0851939A4 (en) 1995-08-30 2001-02-28 New York Medical College METHOD FOR MARKING DNA ENDS WITH HALOGENATED NUCLEOTIDES AND DETECTING THEM WITH ANTIBODIES
US5867266A (en) 1996-04-17 1999-02-02 Cornell Research Foundation, Inc. Multiple optical channels for chemical analysis
FR2755149B1 (fr) * 1996-10-30 1999-01-15 Pasteur Institut Procede de diagnostic de maladies genetiques par peignage moleculaire et coffret de diagnostic
US6403311B1 (en) 1997-02-12 2002-06-11 Us Genomics Methods of analyzing polymers using ordered label strategies
ATE273381T1 (de) 1997-02-12 2004-08-15 Eugene Y Chan Verfahren zur analyse von polymeren
US6117634A (en) 1997-03-05 2000-09-12 The Reagents Of The University Of Michigan Nucleic acid sequencing and mapping
US6197557B1 (en) 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
US20020123063A1 (en) 1997-03-14 2002-09-05 Gjerde Douglas T. Band array display of polynucleotide separations
US6174671B1 (en) 1997-07-02 2001-01-16 Wisconsin Alumni Res Found Genomics via optical mapping ordered restriction maps
US6210896B1 (en) 1998-08-13 2001-04-03 Us Genomics Molecular motors
US6263286B1 (en) 1998-08-13 2001-07-17 U.S. Genomics, Inc. Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
US6790671B1 (en) 1998-08-13 2004-09-14 Princeton University Optically characterizing polymers
US6221592B1 (en) 1998-10-20 2001-04-24 Wisconsin Alumi Research Foundation Computer-based methods and systems for sequencing of individual nucleic acid molecules
US6607888B2 (en) 1998-10-20 2003-08-19 Wisconsin Alumni Research Foundation Method for analyzing nucleic acid reactions
US6438279B1 (en) 1999-01-07 2002-08-20 Cornell Research Foundation, Inc. Unitary microcapiliary and waveguide structure and method of fabrication
EP1157144A4 (en) 1999-01-13 2010-04-28 Cornell Res Foundation Inc MANUFACTURE OF FLUIDER MONOLITHIC STRUCTURES
US20010055764A1 (en) 1999-05-07 2001-12-27 Empedocles Stephen A. Microarray methods utilizing semiconductor nanocrystals
US6635163B1 (en) 1999-06-01 2003-10-21 Cornell Research Foundation, Inc. Entropic trapping and sieving of molecules
US7344627B2 (en) 1999-06-08 2008-03-18 Broadley-James Corporation Reference electrode having a flowing liquid junction and filter members
US6783643B2 (en) 1999-06-22 2004-08-31 President And Fellows Of Harvard College Control of solid state dimensional features
JP2003502166A (ja) 1999-06-22 2003-01-21 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ 固体状態の次元的特徴の制御
US6464842B1 (en) 1999-06-22 2002-10-15 President And Fellows Of Harvard College Control of solid state dimensional features
EP1200474A1 (en) 1999-07-15 2002-05-02 Solvay Pharmaceuticals B.V. Human g-protein coupled receptor
US6762059B2 (en) 1999-08-13 2004-07-13 U.S. Genomics, Inc. Methods and apparatuses for characterization of single polymers
JP2003507026A (ja) * 1999-08-13 2003-02-25 ユー．エス．ジェノミクス，インコーポレーテッド ポリマーを引き伸ばす方法および装置
US6927065B2 (en) 1999-08-13 2005-08-09 U.S. Genomics, Inc. Methods and apparatus for characterization of single polymers
US6696022B1 (en) * 1999-08-13 2004-02-24 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
US7831392B2 (en) 2000-09-28 2010-11-09 Wisconsin Alumni Research Foundation System and process for validating, aligning and reordering one or more genetic sequence maps using at least one ordered restriction map
US7678541B2 (en) 2000-11-21 2010-03-16 Hologic, Inc. Methods and compositions for the detection of a nucleic acid using a non-invasive cleavage reaction
JP4797196B2 (ja) 2001-02-14 2011-10-19 株式会社 フューエンス マイクロチップ
US20020123083A1 (en) * 2001-03-01 2002-09-05 Affymetrix, Inc. Nucleic acid endocing growth factor protein
US7316769B2 (en) 2001-03-19 2008-01-08 Cornell Research Foundation, Inc. Length-dependent recoil separation of long molecules
US20020187508A1 (en) 2001-06-08 2002-12-12 Wong Gordon G. Methods and products for analyzing nucleic acids using nick translation
WO2002101353A2 (en) 2001-06-08 2002-12-19 U.S. Genomics, Inc. Methods and products for analyzing nucleic acids based on methylation status
US20030104428A1 (en) 2001-06-21 2003-06-05 President And Fellows Of Harvard College Method for characterization of nucleic acid molecules
SG169225A1 (en) 2001-07-25 2011-03-30 Univ Princeton Nanochannel arrays and their preparation and use for high throughput macromolecular analysis
JP2005504275A (ja) 2001-09-18 2005-02-10 ユー．エス． ジェノミクス， インコーポレイテッド 高分解能線形解析用のポリマーの差示的タグ付け
WO2003106693A2 (en) 2002-01-01 2003-12-24 Princeton University Gradient structures interfacing microfluidics and nanofluidics, methods for fabrication and uses thereof
US20030215358A1 (en) 2002-01-15 2003-11-20 Schulman Lloyd S. Liquid permeable composition in dry reagent devices
AU2003215391B2 (en) 2002-02-21 2007-05-24 Abbott Diagnostics Scarborough, Inc. Recombinase Polymerase Amplification
US20030162181A1 (en) 2002-02-28 2003-08-28 Eastman Kodak Company DNA sequencing and gene identification
US20030219805A1 (en) 2002-03-13 2003-11-27 Zvi Kelman Detection of alternative and aberrant mRNA splicing
US20040110208A1 (en) 2002-03-26 2004-06-10 Selena Chan Methods and device for DNA sequencing using surface enhanced Raman scattering (SERS)
WO2004016729A2 (en) 2002-08-19 2004-02-26 Bioprocessors Corporation SYSTEMS AND METHODS FOR CONTROL OF pH AND OTHER REACTOR ENVIRONMENTAL CONDITIONS
WO2004048514A2 (en) * 2002-05-09 2004-06-10 U.S. Genomics, Inc. Methods for analyzing a nucleic acid
WO2003095358A2 (en) 2002-05-13 2003-11-20 The Regents Of The University Of Michigan Method of forming manofluidic channels
US7282330B2 (en) 2002-05-28 2007-10-16 U.S. Genomics, Inc. Methods and apparati using single polymer analysis
EP1546380A4 (en) 2002-05-28 2007-02-14 Us Genomics Inc METHODS AND APPARATUSES FOR ANALYZING SIMPLE POLYMERS
US6952651B2 (en) 2002-06-17 2005-10-04 Intel Corporation Methods and apparatus for nucleic acid sequencing by signal stretching and data integration
EP1552013A1 (en) 2002-07-26 2005-07-13 Applera Corporation Microfluidic device including purification column with excess diluent, and method
US7217542B2 (en) 2002-10-31 2007-05-15 Hewlett-Packard Development Company, L.P. Microfluidic system for analyzing nucleic acids
US7312033B2 (en) 2002-11-14 2007-12-25 Third Wave Technologies, Inc. CFTR allele detection assays
US20050250117A1 (en) 2003-10-07 2005-11-10 Xing Su Isolation of single polymeric molecules
GB0324456D0 (en) 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
US20050208538A1 (en) 2003-12-29 2005-09-22 Nurith Kurn Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
US8271251B2 (en) 2004-02-09 2012-09-18 Wisconsin Alumni Research Foundation Automated imaging system for single molecules
US20060014181A1 (en) 2004-06-07 2006-01-19 California Institute Of Technology Detection of DNA mismatches and oxidative lesions
WO2006017274A2 (en) 2004-07-13 2006-02-16 U.S. Genomics, Inc. Systems and methods for sample modification using fluidic chambers
US7511285B2 (en) * 2004-07-16 2009-03-31 The Charles Stark Draper Laboratory, Inc. Methods and apparatus for biomolecule identification
US7351538B2 (en) 2004-08-23 2008-04-01 U.S. Genomics Systems and methods for detecting and analyzing polymers
US20060073489A1 (en) * 2004-10-05 2006-04-06 Gangqiang Li Nanopore separation devices and methods of using same
WO2006098772A2 (en) 2004-10-13 2006-09-21 U.S. Genomics, Inc. Systems and methods for measurement optimization
US20060199202A1 (en) 2005-02-09 2006-09-07 Third Wave Technologies, Inc. Detection of allelic expression imbalance
US20060228734A1 (en) 2005-03-18 2006-10-12 Applera Corporation Fluid processing device with captured reagent beads
US20060275806A1 (en) 2005-05-11 2006-12-07 Schwartz David C Whole genome methylation profiles via single molecule analysis
US20060275911A1 (en) 2005-06-03 2006-12-07 Shih-Yuan Wang Method and apparatus for moleclular analysis using nanostructure-enhanced Raman spectroscopy
US20070128083A1 (en) 2005-07-18 2007-06-07 U.S. Genomics, Inc. Microfluidic methods and apparatuses for sample preparation and analysis
US7960105B2 (en) * 2005-11-29 2011-06-14 National Institutes Of Health Method of DNA analysis using micro/nanochannel
EP3978121A1 (en) 2006-07-19 2022-04-06 BioNano Genomics, Inc. Nanonozzle device arrays: their preparation and use for macromolecular analysis
US8722327B2 (en) 2007-03-28 2014-05-13 Bionano Genomics, Inc. Methods of macromolecular analysis using nanochannel arrays
EP2179285A4 (en) 2007-08-15 2010-08-18 Opgen Inc METHOD, SYSTEM AND SOFTWARE ARRANGEMENT FOR COMPARATIVE ANALYSIS AND PHYLOGENIA WITH OPTICAL TOTAL GENERIC CARD
EP2207901B1 (en) 2007-10-17 2018-07-25 Wisconsin Alumni Research Foundation Methods of whole genome analysis
US7771944B2 (en) 2007-12-14 2010-08-10 The Board Of Trustees Of The University Of Illinois Methods for determining genetic haplotypes and DNA mapping
US20090208950A1 (en) 2008-02-19 2009-08-20 Opgen, Inc. Methods of identifying an organism from a heterogeneous sample
US20090317804A1 (en) 2008-02-19 2009-12-24 Opgen Inc. Methods of determining antibiotic resistance
EP2107040B1 (en) 2008-03-31 2011-10-26 Sony Deutschland Gmbh A method of fabricating a membrane having a tapered pore
EP2288725A4 (en) 2008-05-14 2012-03-21 Opgen Inc METHOD FOR DETERMINING MOLECULE PROPERTIES
WO2011050147A1 (en) 2009-10-21 2011-04-28 Bionanomatrix, Inc . Methods and related devices for single molecule whole genome analysis
US8628919B2 (en) 2008-06-30 2014-01-14 Bionano Genomics, Inc. Methods and devices for single-molecule whole genome analysis
US8835110B2 (en) 2008-11-04 2014-09-16 The Johns Hopkins University DNA integrity assay (DIA) for cancer diagnostics, using confocal fluorescence spectroscopy
JP5846703B2 (ja) 2008-11-18 2016-01-20 バイオナノ ジェノミックス、インク． ポリヌクレオチドのマッピング及び配列決定
SG10201508373UA (en) 2010-10-20 2015-11-27 Bionano Genomics Inc Systems and methods for assessing biomolecule characteristics
US9568425B2 (en) 2011-02-01 2017-02-14 Dna Medicine Institute, Inc. Multicoded analytical nanostrips
CN105143462A (zh) 2013-02-05 2015-12-09 生物纳米基因有限公司 用于单分子分析的方法

Cited By (79)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9051609B2 (en) 2007-10-01 2015-06-09 Nabsys, Inc. Biopolymer Sequencing By Hybridization of probes to form ternary complexes and variable range alignment
US8926813B2 (en) 2008-09-03 2015-01-06 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
US9650668B2 (en) 2008-09-03 2017-05-16 Nabsys 2.0 Llc Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US8871921B2 (en) 2008-09-03 2014-10-28 Quantumdx Group Ltd. Design, synthesis and use of synthetic nucleotides comprising charge mass tags
US8882980B2 (en) 2008-09-03 2014-11-11 Nabsys, Inc. Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US9938573B2 (en) 2008-09-03 2018-04-10 Quantumdx Group Limited Methods and kits for nucleic acid sequencing
US10759824B2 (en) 2008-09-03 2020-09-01 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags
US9410196B2 (en) 2008-09-03 2016-08-09 Quantumdx Group Limited Methods and kits for nucleic acid sequencing
US9719980B2 (en) 2008-09-03 2017-08-01 Nabsys 2.0 Llc Devices and methods for determining the length of biopolymers and distances between probes bound thereto
US9597687B2 (en) 2008-10-10 2017-03-21 Jonas Tegenfeldt Method for the mapping of the local AT/GC ratio along DNA
WO2010042007A1 (en) * 2008-10-10 2010-04-15 Jonas Tegenfeldt Method for the mapping of the local at/gc ratio along dna
US20120040343A1 (en) * 2008-12-19 2012-02-16 Gregory Timp Detecting and Sorting Methylated DNA Using a Synthetic Nanopore
US8394584B2 (en) * 2008-12-19 2013-03-12 The Board Of Trustees Of The University Of Illinois Detecting and sorting methylated DNA using a synthetic nanopore
JPWO2011067961A1 (ja) * 2009-12-02 2013-04-18 独立行政法人科学技術振興機構 流路デバイス及びそれを含むサンプル処理装置
US9114402B2 (en) 2009-12-02 2015-08-25 Japan Science And Technology Agency Channel device and sample treatment apparatus including the same
WO2011067961A1 (ja) * 2009-12-02 2011-06-09 独立行政法人科学技術振興機構 流路デバイス及びそれを含むサンプル処理装置
US8748091B2 (en) 2009-12-18 2014-06-10 The Board Of Trustees Of The University Of Illinois Characterizing stretched polynucleotides in a synthetic nanopassage
US9617595B2 (en) 2010-02-08 2017-04-11 Genia Technologies, Inc. Systems and methods for assembling a lipid bilayer on a substantially planar solid surface
US10343350B2 (en) 2010-02-08 2019-07-09 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US10926486B2 (en) 2010-02-08 2021-02-23 Roche Sequencing Solutions, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US9678055B2 (en) 2010-02-08 2017-06-13 Genia Technologies, Inc. Methods for forming a nanopore in a lipid bilayer
US10371692B2 (en) 2010-02-08 2019-08-06 Genia Technologies, Inc. Systems for forming a nanopore in a lipid bilayer
US9605307B2 (en) 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US11027502B2 (en) 2010-02-08 2021-06-08 Roche Sequencing Solutions, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US10456993B2 (en) 2010-02-08 2019-10-29 Roche Sequencing Solutions, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US10876159B2 (en) 2010-03-03 2020-12-29 Quantum Biosystems Inc. Method and device for identifying nucleotide, and method and device for determining nucleotide sequence of polynucleotide
US10202644B2 (en) 2010-03-03 2019-02-12 Quantum Biosystems Inc. Method and device for identifying nucleotide, and method and device for determining nucleotide sequence of polynucleotide
US9434981B2 (en) 2010-09-27 2016-09-06 Nabsys 2.0 Llc Assay methods using nicking endonucleases
US9702003B2 (en) 2010-11-16 2017-07-11 Nabsys 2.0 Llc Methods for sequencing a biomolecule by detecting relative positions of hybridized probes
US10330633B2 (en) 2011-01-24 2019-06-25 Genia Technologies, Inc. System for communicating information from an array of sensors
EP2668645A4 (en) * 2011-01-24 2017-01-04 Genia Technologies, Inc. System for communicating information from an array of sensors
US9869655B2 (en) 2011-01-24 2018-01-16 Genia Technologies, Inc. System for detecting electrical properties of a molecular complex
US10156541B2 (en) 2011-01-24 2018-12-18 Genia Technologies, Inc. System for detecting electrical properties of a molecular complex
US11274341B2 (en) 2011-02-11 2022-03-15 NABsys, 2.0 LLC Assay methods using DNA binding proteins
US11053535B2 (en) 2011-09-12 2021-07-06 The University Of North Carolina At Chapel Hill Devices with a fluid transport nanochannel intersected by a fluid sensing nanochannel and related methods
US10996212B2 (en) 2012-02-10 2021-05-04 The University Of North Carolina At Chapel Hill Devices and systems with fluidic nanofunnels for processing single molecules
US9989515B2 (en) 2012-02-10 2018-06-05 The University Of North Carolina At Chapel Hill Devices with fluidic nanofunnels, associated methods, fabrication and analysis systems
EP3591408A1 (en) * 2012-02-10 2020-01-08 The University of North Carolina at Chapel Hill Devices with fluidic nanofunnels, associated methods, fabrication and analysis systems
WO2013119765A1 (en) 2012-02-10 2013-08-15 The University Of North Carolina At Chapel Hill Devices with fluidic nanofunnels, associated methods, fabrication and analysis systems
JP2015508889A (ja) * 2012-02-10 2015-03-23 ザ ユニバーシティ オブ ノース カロライナ アット チャペル ヒルＴｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｎｏｒｔｈ Ｃａｒｏｌｉｎａ Ａｔ Ｃｈａｐｅｌ Ｈｉｌｌ 流体ナノファンネルを有する装置、関連する方法、製造及び解析システム
EP2812708A4 (en) * 2012-02-10 2015-09-30 Univ North Carolina DEVICES WITH FLUIDIC NANOTRICHTS, ASSOCIATED METHODS, MANUFACTURE THEREOF AND ANALYSIS SYSTEMS THEREFOR
US10724987B2 (en) 2012-02-27 2020-07-28 Roche Sequencing Solutions, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
US11275052B2 (en) 2012-02-27 2022-03-15 Roche Sequencing Solutions, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
US9535033B2 (en) 2012-08-17 2017-01-03 Quantum Biosystems Inc. Sample analysis method
US9914966B1 (en) 2012-12-20 2018-03-13 Nabsys 2.0 Llc Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation
US10294516B2 (en) 2013-01-18 2019-05-21 Nabsys 2.0 Llc Enhanced probe binding
US10809244B2 (en) 2013-02-05 2020-10-20 Roche Sequencing Solutions, Inc. Nanopore arrays
US9759711B2 (en) 2013-02-05 2017-09-12 Genia Technologies, Inc. Nanopore arrays
US10012637B2 (en) 2013-02-05 2018-07-03 Genia Technologies, Inc. Nanopore arrays
US10669586B2 (en) 2013-02-20 2020-06-02 Bionano Genomics, Inc. Characterization of molecules in nanofluidics
US12275992B2 (en) 2013-02-20 2025-04-15 Bionano Genomics, Inc. Characterization of molecules in nanofluidics
US11359244B2 (en) 2013-02-20 2022-06-14 Bionano Genomics, Inc. Characterization of molecules in nanofluidics
US9809855B2 (en) 2013-02-20 2017-11-07 Bionano Genomics, Inc. Characterization of molecules in nanofluidics
US10844424B2 (en) 2013-02-20 2020-11-24 Bionano Genomics, Inc. Reduction of bias in genomic coverage measurements
US10261065B2 (en) 2013-02-28 2019-04-16 The University Of North Carolina At Chapel Hill Nanofluidic devices with integrated components for the controlled capture, trapping, and transport of macromolecules and related methods of analysis
US11073507B2 (en) 2013-02-28 2021-07-27 The University Of North Carolina At Chapel Hill Nanofluidic devices with integrated components for the controlled capture, trapping, and transport of macromolecules and related methods of analysis
US11307171B2 (en) 2013-03-13 2022-04-19 The University Of North Carolina At Chapel Hill Nanofluidic devices for the rapid mapping of whole genomes and related systems and methods of analysis
US9255288B2 (en) 2013-03-13 2016-02-09 The University Of North Carolina At Chapel Hill Nanofluidic devices for the rapid mapping of whole genomes and related systems and methods of analysis
US10571428B2 (en) 2013-03-13 2020-02-25 The University Of North Carolina At Chapel Hill Nanofluidic devices for the rapid mapping of whole genomes and related systems and methods of analysis
US10106848B2 (en) 2013-03-13 2018-10-23 The University Of North Carolina At Chapel Hill Nanofluidic devices for the rapid mapping of whole genomes and related systems and methods of analysis
US11067537B2 (en) 2013-03-13 2021-07-20 The University Of North Carolina At Chapel Hill Nanofluidic devices for the rapid mapping of whole genomes and related systems and methods of analysis
US9970898B2 (en) 2013-03-13 2018-05-15 The University Of North Carolina At Chapel Hill Nanofluidic devices for the rapid mapping of whole genomes and related systems and methods of analysis
US9618479B2 (en) 2013-03-13 2017-04-11 The University Of North Carolina At Chapel Hill Nanofluidic devices for the rapid mapping of whole genomes and related systems and methods of analysis
US10557167B2 (en) 2013-09-18 2020-02-11 Quantum Biosystems Inc. Biomolecule sequencing devices, systems and methods
US9644236B2 (en) 2013-09-18 2017-05-09 Quantum Biosystems Inc. Biomolecule sequencing devices, systems and methods
US10261066B2 (en) 2013-10-16 2019-04-16 Quantum Biosystems Inc. Nano-gap electrode pair and method of manufacturing same
US10466228B2 (en) 2013-10-16 2019-11-05 Quantum Biosystems Inc. Nano-gap electrode pair and method of manufacturing same
US10393700B2 (en) 2013-10-17 2019-08-27 Roche Sequencing Solutions, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
US10036725B2 (en) 2013-10-17 2018-07-31 Genia Technologies, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
US11773429B2 (en) 2014-02-25 2023-10-03 Bionano Genomics, Inc. Reduction of bias in genomic coverage measurements
US10438811B1 (en) 2014-04-15 2019-10-08 Quantum Biosystems Inc. Methods for forming nano-gap electrodes for use in nanosensors
WO2015170782A1 (en) * 2014-05-08 2015-11-12 Osaka University Devices, systems and methods for linearization of polymers
US10471428B2 (en) 2015-05-11 2019-11-12 The University Of North Carolina At Chapel Hill Fluidic devices with nanoscale manifolds for molecular transport, related systems and methods of analysis
WO2017009710A3 (en) * 2015-07-16 2017-02-23 The Hong Kong University Of Science And Technology Dynamic formation of nanochannels for single-molecule dna analysis
US10722888B2 (en) 2015-07-16 2020-07-28 The Hong Kong University Of Science And Technology Dynamic formation of nanochannels for single-molecule DNA analysis
US11180523B2 (en) 2016-01-04 2021-11-23 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags
US12091712B2 (en) 2016-04-27 2024-09-17 Illumina Cambridge, Ltd. Systems and methods for measurement and sequencing of bio-molecules
EP3984640A1 (en) * 2020-10-16 2022-04-20 Universität Hamburg An autonomous nanofluidic analysis device and a method for the analysis of dna molecules
WO2022078690A1 (en) * 2020-10-16 2022-04-21 Universität Hamburg An autonomous nanofluidic analysis device and a method for the analysis fo dna molecules

Also Published As

Publication number Publication date
JP2014158493A (ja) 2014-09-04
CA2658122A1 (en) 2008-07-03
US20180290885A1 (en) 2018-10-11
EP2049262A2 (en) 2009-04-22
AU2007338862B2 (en) 2014-02-06
CA2658122C (en) 2014-09-02
SG173398A1 (en) 2011-08-29
JP2010510476A (ja) 2010-04-02
US20230126737A1 (en) 2023-04-27
WO2008079169A3 (en) 2008-10-02
US10676352B2 (en) 2020-06-09
US11529630B2 (en) 2022-12-20
US20150323518A1 (en) 2015-11-12
CN103203256A (zh) 2013-07-17
CN103203256B (zh) 2015-09-23
US20210053823A1 (en) 2021-02-25
JP6030599B2 (ja) 2016-11-24
EP3978121A1 (en) 2022-04-06
AU2007338862A1 (en) 2008-07-03
US9061901B2 (en) 2015-06-23
US20090305273A1 (en) 2009-12-10
US9845238B2 (en) 2017-12-19

Similar Documents

Publication Publication Date Title
US20230126737A1 (en) Nanonozzle device arrays: their preparation and use for macromolecular analysis
CA2964611C (en) Methods of macromolecular analysis using nanochannel arrays
US9810680B2 (en) Nanogap electronic detector for measuring properties of a biomolecule stretched in a nanochannel, and method thereof
CN101516512A (zh) 纳米口装置阵列：它们的制备以及在大分子分析中的应用
US20020127855A1 (en) Method for fabricating a pattern in a mask on a surface of an object and product manufactured thereby
AU2014256367A1 (en) Methods of macromolecular analysis using nanochannel arrays
CHANNEL Fatma Doğan

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 200780034694.9

Country of ref document: CN

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 07872156

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: 2007338862

Country of ref document: AU

ENP Entry into the national phase

Ref document number: 2658122

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2009520847

Country of ref document: JP

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 2007338862

Country of ref document: AU

Date of ref document: 20070719

Kind code of ref document: A

REEP Request for entry into the european phase

Ref document number: 2007872156

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2007872156

Country of ref document: EP

DPE1 Request for preliminary examination filed after expiration of 19th month from priority date (pct application filed from 20040101)
NENP Non-entry into the national phase

Ref country code: RU

WWE Wipo information: entry into national phase

Ref document number: 12374141

Country of ref document: US