WO2008064865A3 - Vorrichtung zur durchführung und untersuchung biologischer proben mit temperaturgesteuerten biologischen reaktionen - Google Patents

Vorrichtung zur durchführung und untersuchung biologischer proben mit temperaturgesteuerten biologischen reaktionen Download PDF

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Publication number
WO2008064865A3
WO2008064865A3 PCT/EP2007/010298 EP2007010298W WO2008064865A3 WO 2008064865 A3 WO2008064865 A3 WO 2008064865A3 EP 2007010298 W EP2007010298 W EP 2007010298W WO 2008064865 A3 WO2008064865 A3 WO 2008064865A3
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WO
WIPO (PCT)
Prior art keywords
biochip
reaction chamber
sample solution
carrying
temperature
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Application number
PCT/EP2007/010298
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English (en)
French (fr)
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WO2008064865A2 (de
Inventor
Stefan Heydenhauss
Jens Goehring
Friedrich Menges
Juergen Bauer
Original Assignee
Zenteris Gmbh
Stefan Heydenhauss
Jens Goehring
Friedrich Menges
Juergen Bauer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Zenteris Gmbh, Stefan Heydenhauss, Jens Goehring, Friedrich Menges, Juergen Bauer filed Critical Zenteris Gmbh
Priority to DE112007000683T priority Critical patent/DE112007000683B4/de
Priority to US12/516,612 priority patent/US20100068822A1/en
Publication of WO2008064865A2 publication Critical patent/WO2008064865A2/de
Publication of WO2008064865A3 publication Critical patent/WO2008064865A3/de

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0684—Venting, avoiding backpressure, avoid gas bubbles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/021—Identification, e.g. bar codes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/024—Storing results with means integrated into the container
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/14—Means for pressure control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0605—Valves, specific forms thereof check valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Optical Measuring Cells (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Die Erfindung betrifft eine Vorrichtung zur Durchführung und Untersuchung biologischer Proben mit Temperaturgesteuerten biologischen Reaktionen. Sie umfasst: Eine Reaktionskammer (5) zur Aufnahme eines Biochips (6). Die Reaktionskammer weist zumindest ein transparentes Fenster (14) auf, damit Anregungslicht von außen auf den Biochip (6) gestrahlt werden kann und Fluoressenzlicht vom Biochip nach außen zu einer Messeinrichtung abgestrahlt werden kann. Eine Membran, die zumindest eine Wandung der Reaktionskammer bildet und elastisch ausgebildet ist, so dass das Fenster und der Biochip aneinander drückbar sind, um dazwischen befindliche Probenlösung zu verdrängen. Die erfindungsgemäße Vorrichtung zeichnet sich dadurch aus, dass die Reaktionskammer kommunizierend mit einem Ausgleichsraum verbunden ist. Hierdurch werden in der Reaktionskammer vorbestimmte Druckverhältnisse geschaffen, die zum einen das Verdrängen der Probenlösung vereinfachen und zum anderen die Blasenbildung in der Probenlösung bei hohen Temperaturen verhindern.
PCT/EP2007/010298 2006-11-28 2007-11-27 Vorrichtung zur durchführung und untersuchung biologischer proben mit temperaturgesteuerten biologischen reaktionen WO2008064865A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE112007000683T DE112007000683B4 (de) 2006-11-28 2007-11-27 Vorrichtung zur Durchführung und Untersuchung biologischer Proben mit temperaturgesteuerten biologischen Reaktionen
US12/516,612 US20100068822A1 (en) 2006-11-28 2007-11-27 Device For Carrying Out Tests On And Analyzing Biological Samples With Temperature-Controlled Biological Reactions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102006056540A DE102006056540A1 (de) 2006-11-28 2006-11-28 Vorrichtung und Verfahren zur Untersuchung von biologischen und medizinischen Proben
DE102006056540.1 2006-11-28

Publications (2)

Publication Number Publication Date
WO2008064865A2 WO2008064865A2 (de) 2008-06-05
WO2008064865A3 true WO2008064865A3 (de) 2008-09-12

Family

ID=39111031

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/010298 WO2008064865A2 (de) 2006-11-28 2007-11-27 Vorrichtung zur durchführung und untersuchung biologischer proben mit temperaturgesteuerten biologischen reaktionen

Country Status (3)

Country Link
US (1) US20100068822A1 (de)
DE (2) DE102006056540A1 (de)
WO (1) WO2008064865A2 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102007052281A1 (de) * 2007-11-02 2009-05-07 Zenteris Gmbh Einschritt-Multiplex-Immuntest
DE102008054313B4 (de) 2008-11-03 2012-12-13 Zenteris Gmbh Kartusche und Vorrichtung zur Untersuchung biologischer Proben mit temperaturgesteuerten biologischen Reaktionen
US8840850B2 (en) * 2009-01-15 2014-09-23 Panasonic Corporation Flow channel structure and method of manufacturing same
US8975087B2 (en) * 2010-11-24 2015-03-10 Inanovate, Inc. Longitudinal assay
DE102011109338B3 (de) * 2011-08-03 2013-01-31 Dietrich Reichwein Vorrichtung zur Speicherung elektromagnetischer Energie
WO2014025924A1 (en) * 2012-08-07 2014-02-13 California Institute Of Technology Ultrafast thermal cycler
US10793820B2 (en) * 2013-04-30 2020-10-06 Lawrence Livermore National Security, Llc Miniaturized, automated in-vitro tissue bioreactor
DE102015002000B3 (de) * 2015-02-20 2016-02-04 Friz Biochem Gesellschaft Für Bioanalytik Mbh Mikrofluidische Vorrichtung zur temperaturgesteuerten Verarbeitung einer Probenlösung
DE102015001998B3 (de) * 2015-02-20 2016-02-04 Friz Biochem Gesellschaft Für Bioanalytik Mbh Mikrofluidische Kartusche für den Nachweis von Biomolekülen
GB201518993D0 (en) * 2015-10-27 2015-12-09 Randox Lab Ltd Improvements relating to analysis of biochips
CN106929388A (zh) * 2015-12-31 2017-07-07 苏州百源基因技术有限公司 一种实时荧光定量pcr仪
EP3301431B1 (de) * 2016-09-29 2019-08-28 Roche Diagniostics GmbH Mehrkammer-analysevorrichtung und verfahren zur analyse
IL300862A (en) * 2020-08-26 2023-04-01 Sense Biodetection Ltd instruments
CN114177963B (zh) * 2022-01-13 2024-10-11 深圳市呈晖医疗科技有限公司 核酸分析装置

Citations (8)

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Publication number Priority date Publication date Assignee Title
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WO1997047967A1 (en) * 1996-06-14 1997-12-18 Sarnoff Corporation Automated nucleic acid isolation
FR2790686A1 (fr) * 1999-03-09 2000-09-15 Biomerieux Sa Carte d'analyse dont le remplissage est associe a au moins un volume tampon
FR2803225A1 (fr) * 1999-12-29 2001-07-06 Biomerieux Sa Appareil d'analyse a compartiment reactionnel a geometrie variable, procede de mixage et de guidage de liquides
DE102004023217A1 (de) * 2003-05-12 2004-12-09 Yokogawa Electric Corporation, Musashino Chemisches Reaktionsmodul, dessen Herstellungsverfahren und Antriebssystem für chemisches Reaktionsmodul
WO2005108604A2 (de) * 2004-05-06 2005-11-17 Clondiag Chip Technologies Gmbh Vorrichtung und verfahren zum nachweis von molekularen wechselwirkungen
DE102005019195A1 (de) * 2004-04-28 2005-12-15 Yokogawa Electric Corporation, Musashino Chemische Reaktionspatrone, Verfahren zum Herstellen einer chemischen Reaktionspatrone und Mechanismus zum Betätigen einer chemischen Reaktionspatrone
WO2007051863A2 (en) * 2005-11-04 2007-05-10 Clondiag Gmbh Method and device for the detection of molecular interactions

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US7799521B2 (en) * 1998-06-24 2010-09-21 Chen & Chen, Llc Thermal cycling
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US7470533B2 (en) * 2002-12-20 2008-12-30 Acea Biosciences Impedance based devices and methods for use in assays
US20040137607A1 (en) * 2003-01-09 2004-07-15 Yokogawa Electric Corporation Biochip cartridge
WO2008000770A1 (de) * 2006-06-27 2008-01-03 Zenteris Gmbh Beheizbare reaktionskammer zum prozessieren eines biochips und verfahren zum ansteuern einer solchen reaktionskammer

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4673657A (en) * 1983-08-26 1987-06-16 The Regents Of The University Of California Multiple assay card and system
WO1997047967A1 (en) * 1996-06-14 1997-12-18 Sarnoff Corporation Automated nucleic acid isolation
FR2790686A1 (fr) * 1999-03-09 2000-09-15 Biomerieux Sa Carte d'analyse dont le remplissage est associe a au moins un volume tampon
FR2803225A1 (fr) * 1999-12-29 2001-07-06 Biomerieux Sa Appareil d'analyse a compartiment reactionnel a geometrie variable, procede de mixage et de guidage de liquides
DE102004023217A1 (de) * 2003-05-12 2004-12-09 Yokogawa Electric Corporation, Musashino Chemisches Reaktionsmodul, dessen Herstellungsverfahren und Antriebssystem für chemisches Reaktionsmodul
DE102005019195A1 (de) * 2004-04-28 2005-12-15 Yokogawa Electric Corporation, Musashino Chemische Reaktionspatrone, Verfahren zum Herstellen einer chemischen Reaktionspatrone und Mechanismus zum Betätigen einer chemischen Reaktionspatrone
WO2005108604A2 (de) * 2004-05-06 2005-11-17 Clondiag Chip Technologies Gmbh Vorrichtung und verfahren zum nachweis von molekularen wechselwirkungen
WO2007051863A2 (en) * 2005-11-04 2007-05-10 Clondiag Gmbh Method and device for the detection of molecular interactions

Also Published As

Publication number Publication date
WO2008064865A2 (de) 2008-06-05
DE112007000683B4 (de) 2012-11-15
DE102006056540A1 (de) 2008-05-29
US20100068822A1 (en) 2010-03-18
DE112007000683A5 (de) 2009-08-13

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