WO2008060489A3 - Lignées cellulaires stables et procédés d'évaluation de l'absorption gastro-intestinale de produits chimiques - Google Patents

Lignées cellulaires stables et procédés d'évaluation de l'absorption gastro-intestinale de produits chimiques Download PDF

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Publication number
WO2008060489A3
WO2008060489A3 PCT/US2007/023688 US2007023688W WO2008060489A3 WO 2008060489 A3 WO2008060489 A3 WO 2008060489A3 US 2007023688 W US2007023688 W US 2007023688W WO 2008060489 A3 WO2008060489 A3 WO 2008060489A3
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WO
WIPO (PCT)
Prior art keywords
methods
cell lines
chemicals
gastrointestinal absorption
stable cell
Prior art date
Application number
PCT/US2007/023688
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English (en)
Other versions
WO2008060489A9 (fr
WO2008060489A2 (fr
Inventor
Albert J Owen Iii
Ismael J Hidalgo
Jibin Li
Wei Zhang
Original Assignee
Absorption Systems Group Llc
Albert J Owen Iii
Ismael J Hidalgo
Jibin Li
Wei Zhang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Absorption Systems Group Llc, Albert J Owen Iii, Ismael J Hidalgo, Jibin Li, Wei Zhang filed Critical Absorption Systems Group Llc
Priority to JP2009536326A priority Critical patent/JP5324456B2/ja
Priority to AT07867410T priority patent/ATE485512T1/de
Priority to CA2669013A priority patent/CA2669013C/fr
Priority to EP07867410A priority patent/EP2084529B1/fr
Priority to SI200730479T priority patent/SI2084529T1/sl
Priority to DK07867410.8T priority patent/DK2084529T3/da
Priority to DE602007010019T priority patent/DE602007010019D1/de
Publication of WO2008060489A2 publication Critical patent/WO2008060489A2/fr
Publication of WO2008060489A3 publication Critical patent/WO2008060489A3/fr
Publication of WO2008060489A9 publication Critical patent/WO2008060489A9/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1138—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against receptors or cell surface proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/12—Applications; Uses in screening processes in functional genomics, i.e. for the determination of gene function

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Plant Pathology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

L'invention concerne des acides nucléiques et des vecteurs pour interférer avec l'expression de protéines de transports transmembranaires dans des cellules qui expriment de telles protéines. L'invention concerne également des cellules et des lignées cellulaires comprenant ces acides nucléiques et vecteurs. L'invention concerne également des procédés de criblage de produits chimiques et de biomolécules pour l'absorption gastro-intestinale chez des animaux, et des kits pour mettre en oeuvre de tels procédés.
PCT/US2007/023688 2006-11-10 2007-11-09 Lignées cellulaires stables et procédés d'évaluation de l'absorption gastro-intestinale de produits chimiques WO2008060489A2 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
JP2009536326A JP5324456B2 (ja) 2006-11-10 2007-11-09 化学物質の消化管吸収を評価するための安定的細胞株および方法
AT07867410T ATE485512T1 (de) 2006-11-10 2007-11-09 Stabile zelllinien und verfahren zur beurteilung der aufnahme von chemikalien im magen-darm-trakt
CA2669013A CA2669013C (fr) 2006-11-10 2007-11-09 Lignees cellulaires stables et procedes d'evaluation de l'absorption gastro-intestinale de produits chimiques
EP07867410A EP2084529B1 (fr) 2006-11-10 2007-11-09 Lignées cellulaires stables et procédés d'évaluation de l'absorption gastro-intestinale de produits chimiques
SI200730479T SI2084529T1 (sl) 2006-11-10 2007-11-09 Stabilne celične linije in postopki za evaluacijo gastrointestinalne absorpcije kemikalij
DK07867410.8T DK2084529T3 (da) 2006-11-10 2007-11-09 Stabile cellelinier samt fremgangsmåder til bedømmelse af optagelsen af kemikalier i mavetarmkanalen
DE602007010019T DE602007010019D1 (de) 2006-11-10 2007-11-09 Stabile zelllinien und verfahren zur beurteilung der aufnahme von chemikalien im magen-darm-trakt

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US85793806P 2006-11-10 2006-11-10
US60/857,938 2006-11-10
US89266507P 2007-03-02 2007-03-02
US60/892,665 2007-03-02

Publications (3)

Publication Number Publication Date
WO2008060489A2 WO2008060489A2 (fr) 2008-05-22
WO2008060489A3 true WO2008060489A3 (fr) 2008-10-30
WO2008060489A9 WO2008060489A9 (fr) 2009-06-18

Family

ID=39402218

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/023688 WO2008060489A2 (fr) 2006-11-10 2007-11-09 Lignées cellulaires stables et procédés d'évaluation de l'absorption gastro-intestinale de produits chimiques

Country Status (10)

Country Link
US (4) US7795019B2 (fr)
EP (1) EP2084529B1 (fr)
JP (3) JP5324456B2 (fr)
AT (1) ATE485512T1 (fr)
CA (1) CA2669013C (fr)
DE (1) DE602007010019D1 (fr)
DK (1) DK2084529T3 (fr)
PA (1) PA8755401A1 (fr)
SI (1) SI2084529T1 (fr)
WO (1) WO2008060489A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100047907A1 (en) * 2008-08-19 2010-02-25 Absorption System Group LLC Methods of transporting epithelial cell monolayers
CA2821547A1 (fr) 2010-12-29 2012-07-05 Sigma-Aldrich Co. Llc Cellules dont l'expression des proteines impliquees dans les processus adme et toxicologiques est perturbee

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006021894A2 (fr) * 2004-08-26 2006-03-02 Engeneic Gene Therapy Pty Limited Administration d'acides nucleiques fonctionnels dans des cellules de mammiferes via de minicellules intactes derivees par bacteries

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU217108B (hu) * 1994-08-31 1999-11-29 SOLVO Biotechnológiai Kft. Eljárás daganatok multidrogrezisztenciáját okozó fehérje aktivitásának in vitro mennyiségi kimutatására biológiai mintákban
US7601494B2 (en) * 1999-03-17 2009-10-13 The University Of North Carolina At Chapel Hill Method of screening candidate compounds for susceptibility to biliary excretion
US20050048468A1 (en) * 2003-08-27 2005-03-03 Bio-Rad Laboratories, Inc. Multiconstituent liquid lgG and IgM calibrators
JP2007528730A (ja) * 2004-03-12 2007-10-18 ザ クイーンズ ユニヴァーシティ オブ ベルファスト 治療および測定法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006021894A2 (fr) * 2004-08-26 2006-03-02 Engeneic Gene Therapy Pty Limited Administration d'acides nucleiques fonctionnels dans des cellules de mammiferes via de minicellules intactes derivees par bacteries

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CELIUS T ET AL: "Stable suppression of MDR1 gene expression and function by RNAi in Caco-2 cells", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 324, no. 1, 5 November 2004 (2004-11-05), pages 365 - 371, XP004605944, ISSN: 0006-291X *
LEE ET AL: "siRNA-Getting the message out", EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, ELSEVIER, AMSTERDAM, NL, vol. 27, no. 5, 1 April 2006 (2006-04-01), pages 401 - 410, XP005331255, ISSN: 0928-0987 *
MORRIS KEVIN V ET AL: "Lentivirus-mediated RNA interference therapy for human immunodeficiency virus type 1 infection.", HUMAN GENE THERAPY MAY 2006, vol. 17, no. 5, May 2006 (2006-05-01), pages 479 - 486, XP002491626, ISSN: 1043-0342 *
STEGE ALEXANDRA ET AL: "Stable and complete overcoming of MDR1/P-glycoprotein-mediated multidrug resistance in human gastric carcinoma cells by RNA interference.", CANCER GENE THERAPY NOV 2004, vol. 11, no. 11, November 2004 (2004-11-01), pages 699 - 706, XP002491625, ISSN: 0929-1903 *
TOMOKO WATANABE ET AL: "Construction of a Functional Transporter Analysis System Using MDR1 Knockdown Caco-2 Cells", PHARMACEUTICAL RESEARCH, KLUWER ACADEMIC PUBLISHERS-PLENUM PUBLISHERS, NE, vol. 22, no. 8, 1 August 2005 (2005-08-01), pages 1287 - 1293, XP019370909, ISSN: 1573-904X *
WIZNEROWICZ MACIEJ ET AL: "Tuning silence: conditional systems for RNA interference.", NATURE METHODS SEP 2006, vol. 3, no. 9, September 2006 (2006-09-01), pages 682 - 688, XP002491627, ISSN: 1548-7091 *

Also Published As

Publication number Publication date
EP2084529A2 (fr) 2009-08-05
PA8755401A1 (es) 2009-07-23
SI2084529T1 (sl) 2011-02-28
JP2013240340A (ja) 2013-12-05
US8110398B2 (en) 2012-02-07
US20100311082A1 (en) 2010-12-09
US20120142008A1 (en) 2012-06-07
ATE485512T1 (de) 2010-11-15
US20100317049A1 (en) 2010-12-16
WO2008060489A9 (fr) 2009-06-18
DE602007010019D1 (de) 2010-12-02
JP2013240341A (ja) 2013-12-05
EP2084529B1 (fr) 2010-10-20
CA2669013C (fr) 2015-04-14
WO2008060489A2 (fr) 2008-05-22
DK2084529T3 (da) 2011-02-07
US20080124734A1 (en) 2008-05-29
US8105828B2 (en) 2012-01-31
JP5324456B2 (ja) 2013-10-23
CA2669013A1 (fr) 2008-05-22
US7795019B2 (en) 2010-09-14
JP2010508849A (ja) 2010-03-25

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