WO2008040144A1 - Substrat de frottis sanguin et dispositif de frottis sanguin - Google Patents

Substrat de frottis sanguin et dispositif de frottis sanguin Download PDF

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Publication number
WO2008040144A1
WO2008040144A1 PCT/CN2007/002612 CN2007002612W WO2008040144A1 WO 2008040144 A1 WO2008040144 A1 WO 2008040144A1 CN 2007002612 W CN2007002612 W CN 2007002612W WO 2008040144 A1 WO2008040144 A1 WO 2008040144A1
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WO
WIPO (PCT)
Prior art keywords
smear
blood
station
tape
drying
Prior art date
Application number
PCT/CN2007/002612
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English (en)
French (fr)
Inventor
Honghua Zhou
Weijiang Lu
Xianghua Li
Minsheng Zhu
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Tecom Science Corporation
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Publication date
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Publication of WO2008040144A1 publication Critical patent/WO2008040144A1/zh

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on

Definitions

  • Blood smear base and blood smear device Blood smear device
  • the present invention relates to the field of blood cell analysis, and more particularly to a smear device for a blood cell analyzer.
  • BACKGROUND OF THE INVENTION Blood smears are the basic method of routine blood testing and are the basic method of operation relied upon by the International Hematology Standards Committee for reference methods for blood analysis standards. The traditional method is usually made by hand. The method is to first push the blood on a glass slide, then dry, dye, dry, and then perform microscopic observation and counting. This manual operation is slow and not suitable for large-volume blood samples.
  • a "blood smear machine” which drives a reciprocating motion of a propeller by a motor, and pushes the slide to push the slide into the smear, drying and dyeing process, respectively.
  • the device is provided with a push plate with a blood drop hole, a drying box and a dyeing tank, and the slides are respectively smeared, dried and dyed.
  • the blood smear machine is a method of pushing a single slide glass, and the structure thereof is not compact, and only the reciprocating injection, sampling, and dyeing processes must be manually operated, and continuous strip injection and sampling are not possible.
  • the object of the present invention is to provide a substrate for a blood smear, and a blood smear machine for designing a continuous strip sample and a sample using the substrate of the blood smear, thereby effectively improving the blood smear machine. degree of automation.
  • the present invention is solved by the following means:
  • the transparent tape of the present invention may be a transparent tape with perforations or a transparent tape without perforations, depending on the manner in which the transparent tape is driven, that is, the mechanism of the transmission.
  • the present invention is described below with a perforated transparent tape.
  • the transparent tape is driven by the teeth of the gear to drive the transparent tape with the perforations continuously.
  • this description does not refer to the scotch tape of the present invention, but is a scotch tape with perforations. Scotch tape without perforations can be used when other transmission mechanisms such as drums are used.
  • the scotch tape of the present invention may use a film tape or other scotch tape.
  • it is required to have good optical characteristics, is not easily broken during the transmission process, and can perform corresponding blood samples thereon. Smear, dye, dry, etc.
  • a transparent tape is used as a substrate for a blood smear.
  • the present invention provides a method for treating a single blood sample slide by smear, dyeing and drying one by one.
  • the new smear, dyeing and drying methods are used to process the smear, drying, dyeing and drying processes prepared by blood smears on a continuous flow line.
  • the scotch tape proposed for the present invention is a perforated transparent tape, and the present invention can be realized by the following specific technical solutions:
  • the preparation of the single blood smear of the invention is also completed by four steps of smear, drying, dyeing and drying, and the smear process can be completed by using the existing blood smear technique (device), that is, the existing
  • the blood sample smear device can be used as an essential component of the blood smear device of the present invention.
  • the three steps of drying, dyeing and drying after the smear of the invention are respectively completed in three sealed sealed boxes formed by the upper insulating cover (3) and the lower insulating cover (4), that is, the upper isolation
  • the three sealed sealing boxes formed when the cover (3) and the lower insulating cover (4) are closed are the components of the three processes of the present invention for drying, dyeing and drying, that is, the three basic components of the blood smear device.
  • the invention is mainly composed of a gear (2) and a spacer, wherein the gear (2) is driven by power through a perforated transparent tape (hereinafter referred to as a scotch tape) (1) (in another row of scotch tape)
  • a perforated transparent tape hereinafter referred to as a scotch tape
  • the spacer is composed of an upper cover (3) and a lower cover (4), the upper cover (3) is above the transparent tape, and the lower cover (4) is below the transparent tape, when is isolated When the cover (3) is separated from the lower cover (4) ( Figure 3), the transparent tape moves forward.
  • the upper isolation cover (3) and the lower isolation cover (4) are closed (as shown in Fig. 4), and the upper and lower isolation cover are boxes corresponding to the three upper and lower positions.
  • the cavity structure is configured.
  • the upper and lower isolation covers are closed, the upper and lower half-box chambers of the upper and lower positions of the upper and lower isolation covers are closed to form three independent sealed boxes, and corresponding The corresponding part of the scotch tape of the station is completely sealed in the sealed box, thus forming three independent blood sample smear processing stations, wherein E and E "constitute the drying box of the drying station, F and F" constitute The dyeing box of the dyeing station, G and G ⁇ constitute a drying box for drying work.
  • the drying box composed of £ and ⁇ , G and G" is provided with a hot air inlet hole and an air outlet hole, and the hot air inlet hole is connected with the external hot air source, and the dry hot air enters, and the air outlet hole can be opened. It may also be connected to a vacuum device to discharge water vapor.
  • the dyeing box formed by F and F" is provided with a dyeing liquid injection hole and a discharge hole, and the dyeing liquid is injected from the injection hole. After the blood smear is dyed, the dyeing liquid is discharged from the discharge hole, and then the dyed sheet is washed and drained. Finally, the water drops that are not drained are blown off by the high pressure gas.
  • the blood smear device of the present invention can be further described by the following description of the operation of the device.
  • the gear (2) passes through the perforations of the transparent tape, and drives the transparent tape to move forward to a station.
  • the upper cover (3) and the lower cover (4) are closed and sealed, and the device first uses the existing mechanical blood sample smear means.
  • the smear operation of the blood sample smear on the scotch tape portion of the A station the upper hood (3) is separated from the lower hood (4), the gear (2) drives the scotch tape to move forward one station, so that the scotch tape
  • the blood sample smear is displaced to the B station;
  • the upper isolation cover (3) and the lower isolation cover (4) are closed and sealed, and the blood sample smear portion of the transparent adhesive tape is dried in a dry box composed of E and E ⁇ , and the system performs blood sample on the transparent tape portion of the A station.
  • the smear operation the upper spacer (3) is separated from the lower spacer (4), the gear (2) is passed through the perforation of the transparent tape, and the transparent tape is moved to move forward by a station, so that the scotch tape blood sample is dried.
  • the rear part enters the C station, and the corresponding transparent tape just enters the B station for the operation of the blood sample smear;
  • the upper isolation cover (3) and the lower isolation cover (4) are then closed and sealed, in? versus?
  • the sealed dyeing box composed of ⁇ the system dyes the blood sample smear portion of the dried scotch tape, and in the dry box composed of E and E", the blood sample smear portion of the scotch tape which is inserted into the B station Drying is performed, and at the same time, the system performs a smear operation of the blood sample into the transparent tape portion of the A station.
  • the upper cover (3) is separated from the lower cover (4), and the gear (2) passes through the perforation of the transparent tape.
  • the upper isolation cover (3) and the lower isolation cover (4) are closed again and sealed, and the system is dried in the dry box formed by G and G ⁇ , and the smear portion of the blood sample after the previous process of the transparent tape is dried.
  • the dyeing box composed of F ⁇ the blood sample smear portion after the drying process of the transparent tape is dyed, and the blood sample after the smear operation in the previous step of the transparent tape in the dry box formed by E and E"
  • the smear part is dried.
  • the smear part of the scotch tape at the station is smeared, the upper hood (3) and the lower hood (4) are separated again, and the scotch tape is moved forward one more. Station.
  • the blood smear device completes a working sequence, that is, the blood smear device has completed a complete process of smear, drying, dyeing, drying on scotch tape, smeared, dried,
  • the blood smear of the scotch tape after dyeing and drying can be subjected to subsequent operations such as microscopic observation and counting.
  • the auto-smear operation of the blood smear is achieved as the scotch tape moves forward in accordance with the working beat and repeats the above-described working process.
  • the blood smear device of the present invention is not limited to the treatment of a blood smear having a scotch tape as a base, and in addition to using the blood smear device of the present invention to treat a smear of a scotch tape as a base, Other methods can be used to treat the smear of the scotch tape as a substrate.
  • each station can be treated in an open manner, the smear of the A station is unchanged, and the two drying stations B and D are non-closed.
  • the hood type heat source, and the C station dyeing treatment can adopt a concave groove structure.
  • the blood smear portion of the scotch tape is pressed into the groove containing the dye liquor to perform dyeing.
  • a closed dyeing process that retains the C station can be used, and other stations use an open operation or the like.
  • the lower spacer (4) does not have to have a concave groove structure or a flat plate structure.
  • the upper spacer (3) and the lower spacer (4) are not necessarily integral structures, but may be separate and separate structures.
  • FIG. 1 is a schematic view of a perforated transparent tape of the present invention.
  • 2 is a schematic view of a station of a blood smear device of the present invention.
  • 1 is a transparent tape with perforations
  • 2 is a transmission gear (in the position of another row of perforations of the transparent tape, there is also a gear that is coaxial with the gear 2 and has the same structure, not shown in the figure, the following figures Same)
  • A is the smear station
  • B is the drying station
  • C is the dyeing station
  • D is the drying station.
  • 3 is a longitudinal cross-sectional view of the position of the transmission gear 2 when the upper and lower parts of the isolation cover of the blood smear device of the present invention are separated, 1 is a transparent tape, 2 is a transmission gear, 3 is an upper isolation cover, 4 is a lower Isolation cover, E, F, G are the three box cavities of the upper isolation cover, E “ , F “ , G “ respectively are the three cassette cavities of the lower isolation cover, the positions of E ⁇ , F “ , G " respectively Corresponding to E, F, G of the upper isolation cover, A is the smear station, B is the drying station, C is the dyeing station, and D is the drying station.
  • Fig. 4 is a longitudinal cross-sectional view showing the position of the transmission gear 2 when the upper and lower portions of the spacer of the blood smear device of the present invention are closed and sealed.
  • 1 is transparent tape
  • 2 is transmission gear
  • 3 is upper isolation cover
  • E, F, G are respectively three box cavities of upper isolation cover, E ⁇ , F ⁇ , G" respectively for lower isolation
  • the positions of the three chambers of the cover, E ⁇ , F", G" correspond to the E, F, G of the upper isolation cover respectively
  • A is the smear station
  • B is the drying station
  • C is the dyeing station.
  • D is the drying station.
  • Figure 5 is a cross-sectional view of the HH surface of Figure 4 of the blood smear device of the present invention
  • 1 is a transparent tape
  • 3 is an upper isolation cover
  • 4 is a lower isolation cover
  • G is a box cavity of the upper isolation cover
  • G is a lower isolation cover
  • FIG. 1 is a schematic view of a perforated transparent tape of a base-scotch tape of a blood smear according to the present invention.
  • the two edges of the embodiment are respectively engraved with a perforated strip, and in use, the two are Parallel perforated belts, through the perforations, respectively, the gears (2) shown in Figure 2 and the gears corresponding to the other rows of perforations of the scotch tape not shown in the figure, and according to the set work The beat moves forward.
  • Example 2
  • FIG 3, 4, and 5 are schematic views of a blood smear device according to the present invention.
  • the blood smear device is composed of a gear (2) and a spacer, wherein the gear (2) is driven by power, and passes through a perforated transparent tape (hereinafter referred to as a scotch tape) (1).
  • a perforated transparent tape hereinafter referred to as a scotch tape
  • the spacer is composed of the upper cover (3) and the lower cover (4).
  • the upper cover (3) is above the transparent tape.
  • the isolation cover (4) is under the transparent tape.
  • the upper and lower isolation cover are composed of three upper and lower positions corresponding to the box cavity structure, when the upper and lower isolation covers are When closed, the upper and lower halves of the upper and lower positions of the upper and lower isolation covers are closed, forming three independent sealed boxes, and the corresponding parts of the transparent tape of the corresponding station are completely sealed.
  • the sealed box thus forming three independent The blood sample smear processing station, in which E and E "constitute a drying box for the drying station, F and F ⁇ constitute a dyeing box for the dyeing station, and 0 and 6 ⁇ constitute a drying box for drying work.
  • the gear (2) moves the transparent tape forward through the perforation of the transparent tape, and the upper cover (3) and the lower cover (4) are closed and sealed, and the system performs blood sampling on the transparent tape portion of the A station.
  • the smear operation the upper isolation cover (3) is separated from the lower isolation cover (4), the gear (2) drives the transparent adhesive tape to move forward one station, and the blood sample smear portion of the transparent adhesive tape is displaced to the B station; (3) Closed and sealed with the lower isolation cover (4), the blood sample smear portion of the transparent tape is dried in a dry box composed of E and E", and the system performs blood sample smear on the transparent tape portion of the A station.
  • the upper isolation cover (3) is separated from the lower isolation cover (4), and the gear (2) is passed through the perforation of the transparent adhesive tape to drive the transparent adhesive tape and then move forward by a station, so that the scotch tape blood sample smear is dried.
  • the part enters the C station, and the corresponding transparent tape just enters the B station for the operation of the blood sample smear;
  • the upper isolation cover (3) and the lower isolation cover (4) are closed and sealed, and in the closed dyeing box composed of F and F", the system dyes the blood sample smear portion of the dried transparent tape, in E
  • the smear portion of the smear of the scotch tape entering the B station is dried in the dry box formed by the E ,, and the smear operation of the blood sample is performed on the scotch tape portion of the A station, and the upper cover (3) and the lower part are placed.
  • the spacer (4) is split, and the gear (2) passes through the perforations of the transparent tape.
  • the upper isolation cover (3) and the lower isolation cover (4) are closed again and sealed, and the system is dried in the dry box formed by G and G", and the smear portion of the blood sample after the previous process of the transparent tape is dried.
  • the dyeing box made of F ⁇ the blood sample smear portion after the drying process of the transparent tape is dyed, and the blood sample after the smear operation of the transparent tape in the previous process in the dry box composed of E and E"
  • the smear part is dried.
  • the smear part of the scotch tape at the station is smeared.
  • the upper hood (3) and the lower hood (4) are separated again, and the scotch tape is moved forward one more. Station.
  • the blood smear device completes a working sequence, that is, the blood smear device has completed a complete process of smear, smear, drying, dyeing and drying on the scotch tape, smear, dry
  • the blood smear of the scotch tape after dyeing and drying can be subjected to subsequent operations such as microscopic observation and counting.
  • the auto-smear operation of the blood smear is achieved as the scotch tape moves forward in accordance with the working beat and repeats the above-described working process.

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Description

一种血涂片的基底及血涂片装置
技术领域 本发明涉及血细胞分析领域， 尤其是涉及血细胞分析仪的涂片装置。 背景技术 血液涂片是进行血常规检验的基本方法，也是国际血液学标准委员会 规定的血液分析标准参考方法所依赖的基本操作方法。 其传统的方式多采 用手工制作， 其方法是先在一张载玻片上进行血液推片， 然后干燥、染色、 干燥， 再进行显微观察、 计数等操作。 这种人工操作方式速度慢， 不适合 大批量的血样处理。
公开号为 CN2153056的实用新型专利中， 公开了一种 "血液涂片机"， 这种装置通过马达带动推进尺往复运动， 推进尺再推动载玻片分别进入涂 片、 烘干、 染色工序， 装置设有带滴血孔的推板、 烘干箱、 染色缸， 分别 对载玻片进行涂片、 烘干、 染色处理。 该血液涂片机均为推单片载玻片的 方式， 其结构不紧凑， 只能往复式进样、 出样， 染色后的工序， 必须人工 操作， 不能连续带状进样、 出样。
现有的血液涂片机中， 都是将单一的血样载玻片经涂片、染色、千燥， 再进行观察、 处理。 要么就是自动化程度不高， 要么就是系统庞大、 结构 复杂。 发明内容 本发明的目的是提出一种血涂片的基底， 以及利用这种血涂片的基 底， 设计出连续带状进样、 出样的血涂片机， 有效地提高血涂片机的自动 化程度。
本发明是通过以下途径解决的：
改变现有技术中血涂片机对单一的血样载玻片处理的方式， 用透明胶 带替代单一的血样载玻片， 在透明胶带上作血样涂片、染色、干燥等处理， 并通过透明胶带的有节奏、按工序的连续移动，实现血样涂片的连续处理。 本发明所说的透明胶带， 可以是带齿孔的透明胶带， 也可以是不带齿 孔的透明胶带， 视透明胶带传动的方式， 也即传动的机构的不同而不同。 本发明以下要叙述的是带齿孔的透明胶带， 其透明胶带的传动方式是通过 齿轮的齿， 带动带齿孔的透明胶带连续走样。 但是， 这种叙述并非是指本 发明的透明胶带， 只是带齿孔的透明胶带。 在采取其它传动机构如卷桶式 时， 可以采用不带齿孔的的透明胶带。
本发明所说的透明胶带可以使用胶片带， 也可以是其它透明胶带， 就 本发明而言， 要求是具有良好光学特性， 在传动过程中不易被拉断， 并能 够在其上进行相应的血样涂片、 染色、 干燥等操作。
针对本发明提出的用透明胶带作为血涂片的基底方案， 本发明一改现 有技术中， 对单一的血样载玻片进行逐一的血样涂片、 染色、 干燥的处理 方式， 提出了一种全新的涂片、 染色、 干燥的处理方式， 将血涂片制备的 涂片、干燥、染色、干燥四道工序放在一个连续的流水作业线上进行处理。 针对本发明提出的透明胶带为带齿孔的透明胶带， 本发明可以由以下具体 的技术方案来实现：
本发明单一血涂片的制备， 也是通过涂片、 干燥、 染色、 干燥四道工 序完成的， 其涂片工序可以采用现有的血样涂片技术 （装置） 完成， 也就 是说， 现有的血样涂片装置， 可以作为本发明的血涂片装置的一个基本部 件。
本发明涂片之后的干燥、染色、干燥三道工序是分别在由上隔离罩（3) 和下隔离罩 （4) 闭合时形成三个隔离的密封盒内完成的， 也就是说， 上 隔离罩 （3) 和下隔离罩 （4) 闭合时形成三个隔离的密封盒是本发明处理 干燥、 染色、 干燥三道工序的部件， 也就是血涂片装置的三个基本部件。
本发明主要是由齿轮 （2)、 隔离罩构成， 其中齿轮 （2) 由动力驱动， 通过带齿孔的透明胶带 （以下简称透明胶带） （1 ) 的齿孔 （在透明胶带的 另一排齿孔的位置上， 还有一个与齿轮 2同轴且结构相同的齿轮， 两个齿 轮分别作用于透明胶带的两排齿孔）， 带动透明胶带向前 （图中为由右向 左方向）按工作节拍移动， 隔离罩由上隔离罩（3 )和下隔离罩（4)组成， 上隔离罩 （3) 在透明胶带的上方， 下隔离罩 （4) 在透明胶带的下方， 当 上隔离罩 （3) 与下隔离罩 （4) 分开 （如图 3 ) 时， 透明胶带向前移动一 个工位， 移动到相应的工位上后，上隔离罩（3)与下隔离罩（4)闭合（如 图 4)，上、下隔离罩是由三个上、下位置相对应的盒式腔结构构成，当上、 下隔离罩闭合时， 上、 下隔离罩的三个上、 下位置相对应的上、 下半盒式 腔闭合， 形成三个相互独立的密封盒， 并将相应工位的透明胶带的相应部 位，完全密闭在密封盒内，从而形成了三个相互独立的血样涂片处理工位， 其中 E与 E "构成了干燥工位的干燥盒， F与 F"构成了染色工位的染色盒， G与 G〃构成了干燥工作的干燥盒。
£与£〃构成的干燥盒、 G与 G"构成的干燥盒上设有热风进风孔和出 气孔， 热风进风孔与外热风源相连接， 干燥热风由此进入， 出气孔可以是 敞开的， 也可以是与一负压装置相连接， 排出水蒸气。
F与 F"构成的染色盒上设有染液注入孔和排出孔， 染液由注入孔注 入，血涂片染色完成后， 染液由排出孔排出， 然后对染片进行冲洗、排干， 最后通过高压气体将没有排掉的水珠吹掉。
本发明所述的血涂片装置， 可以通过以下对该装置的工作过程叙述， 作进一步的说明。
齿轮 （2)通过透明胶带的齿孔， 带动透明胶带向前移动一个工位， 上隔离罩（3)与下隔离罩（4)合拢并密闭， 装置先用现有的机械血样涂 片手段， 对处于 A工位的透明胶带部位进行血样涂片的涂片操作， 上隔离 罩 （3) 与下隔离罩（4) 分开， 齿轮 （2) 带动透明胶带向前移动一个工 位， 使透明胶带的血样涂片部位移到 B工位；
上隔离罩 （3)与下隔离罩（4)合拢并密闭， 透明胶带的血样涂片部 位处于 E与 E〃构成的干燥盒内进行干燥处理， 同时系统对进入 A工位透 明胶带部位进行血样的涂片操作， 上隔离罩（3) 与下隔离罩 （4)分开， 齿轮 （2 ) 通过透明胶带的齿孔， 带动透明胶带再向前移动一个工位， 使 得透明胶带血样涂片干燥处理后的部位进入 C工位， 相应的透明胶带刚作 血样涂片操作的部位进入 B工位；
上隔离罩 （3) 与下隔离罩 （4)再合拢并密闭， 在？与？〃构成的密 闭的染色盒中， 系统对干燥处理后的透明胶带的血样涂片部位进行染色处 理， 在 E与 E"构成的干燥盒内对迸入 B工位的透明胶带的血样涂片部位 进行干燥处理，同时系统对进入 A工位透明胶带部位进行血样的涂片操作， 上隔离罩 （3) 与下隔离罩 （4) 分开， 齿轮 （2)通过透明胶带的齿孔， 带动透明胶带再向前移动一个工位， 使得透明胶带血样涂片染色处理后的 部位进入 D工位， 干燥处理后的部位进入 C工位， 涂片操作后的部位进入 B工位；
上隔离罩 （3 ) 与下隔离罩 （4) 再次合拢并密闭， 系统在 G与 G〃构 成的干燥盒内， 对透明胶带前次工序染色处理后的血样涂片部位进行干燥 处理， 在 F与 F〃 构成的染色盒内， 对透明胶带前次工序干燥处理后的血 样涂片部位进行染色处理， 在 E与 E " 构成的干燥盒内， 对透明胶带前次 工序涂片操作后的血样涂片部位进行干燥处理， 在 A工位， 对处于该工位 的透明胶带的部位进行涂片操作， 上隔离罩 （3 ) 与下隔离罩 （4) 再次分 开， 透明胶带再向前移动一个工位。
通过以上操作， 血涂片装置完成了一个工作序列， 也就是说， 血涂片 装置已经完成了在透明胶带上， 涂片、 干燥、 染色、 干燥的一个完整的过 程， 经涂片、 干燥、 染色、 干燥处理后的透明胶带的血涂片， 可以进入显 微观察、 计数等后续的操作。 而随着透明胶带按工作节拍的向前移动， 重 复以上所述的工作过程， 就实现了血涂片的自动化涂片操作。
本发明所说的血涂片装置， 并不是对透明胶带作为基底的血涂片的处 理的限制， 除了使用本发明所述的血涂片装置来处理透明胶带作为基底的 血涂片外， 还可以使用其它方式来处理透明胶带作为基底的血涂片， 如， 各工位可以采用敞开式的处理方式， A工位的涂片方式不变， B和 D两个 干燥工位采用非封闭的罩式热源， 而 C工位的染色处理， 可以采用一个下 凹的槽结构， 透明胶带进入该工位时， 将透明胶带的血涂片部位压入盛有 染液的凹槽中， 进行染色处理。 又如， 可以采用保留 C工位的封闭式染色 处理， 其它工位都采用敞开式的操作等等。 再如， 下隔离罩 （4) 不一定 要有下凹的槽结构， 也可是平板结构。 再又如， 上隔离罩 （3 ) 与下隔离 罩 （4 ) 不一定是整体结构， 也可是各自分开的独立结构。 这些不同的处 理处理方式， 都可以较好地达到处理透明胶带作为基底的血涂片的目的。
本发明采用透明胶带作为血涂片的基底， 有效地提高了血涂片机的自 动化连续作业的程度， 同时使血涂片的处理， 更加简单、 有效， 使血涂片 装置的设计制造更为简洁， 结构更为紧凑， 系统处理更为方便， 血涂片处 理更为流畅， 而涂片、 染色、 千燥工位的相对独立和相互隔离， 避免了各 工位之间的相互干扰， 同时避免了对周围环境的污染。 附图说明 图 1为本发明的一种带齿孔的透明胶带的示意图。
图 2为本发明的一种血涂片装置的工位示意图。 1为带齿孔的透明胶 带， 2为传动齿轮 （在透明胶带的另一排齿孔的位置上， 还有一个与齿轮 2同轴且结构相同的齿轮， 图中未标示出来，下列各图同）， A为涂片工位， B为干燥工位， C为染色工位， D为干燥工位。
图 3为本发明一种血涂片装置的隔离罩上、 下两部分分开时传动齿轮 2位置上的纵向剖面示意图， 1为透明胶带， 2为传动齿轮， 3为上隔离罩， 4为下隔离罩， E、 F、 G分别为上隔离罩的三个盒式腔， E " 、 F" 、 G "分 别为下隔离罩的三个盒式腔， E〃 、 F " 、 G"的位置分别与上隔离罩的 E、 F、 G相对应， A为涂片工位， B为干燥工位， C为染色工位， D为干燥工位。
图 4为本发明一种血涂片装置的隔离罩上、 下两部分合拢并密闭时传 动齿轮 2位置上的纵向剖面示意图。 1为透明胶带， 2为传动齿轮， 3为上 隔离罩， 为下隔离罩， E、 F、 G分别为上隔离罩的三个盒式腔， E〃 、 F 〃 、 G"分别为下隔离罩的三个盒式腔， E〃 、 F " 、 G" 的位置分别与上隔 离罩的 E、 F、 G相对应， A为涂片工位， B为干燥工位， C为染色工位， D 为干燥工位。
图 5为本发明血涂片装置图 4的 H-H面的剖视图， 1为透明胶带， 3 为上隔离罩， 4为下隔离罩， G为上隔离罩的盒式腔， G "为下隔离罩的盒 式腔。 具体实施例 本发明将通过以下实施例作进一步的说明。
实施例 1
图 1 为本发明所述的血涂片的基底 -透明胶带的一种带齿孔的透明胶 带的示意图， 本实施例的两个边缘分别刻有一条齿孔带， 在使用时， 这两 条平行的齿孔带， 通过齿孔， 分别由图 2所示的齿轮 （2 )和图中未标示 出来的与透明胶带的另一排齿孔位置相对应的齿轮传动， 并按设定的工作 节拍向前移动。 实施例 2
图 3、 图 4、 图 5是本发明所述的一种血涂片装置的示意图。
本实施例中， 血涂片装置是由齿轮（2)、 隔离罩构成， 其中齿轮（2) 由动力驱动， 通过带齿孔的透明胶带 （以下简称透明胶带 )·（1 ) 的齿孔， 带动透明胶带向前（图为由右向左方向）按工作节拍移动， 隔离罩由上隔 离罩 （3 ) 和下隔离罩 （4)组成， 上隔离罩 （3) 在透明胶带的上方， 下 隔离罩 （4)在透明胶带的下方， 当上隔离罩（3 ) 与下隔离罩（4) 分幵 (如图 3) 时， 透明胶带向前移动一个工位， 移动到相应的工位上后， 上 隔离罩 （3)与下隔离罩（4) 闭合（如图 4)， 上、 下隔离罩是由三个上、 下位置相对应的盒式腔结构构成， 当上、 下隔离罩闭合时， 上、 下隔离罩 的三个上、 下位置相对应的上、 下半盒式腔闭合， 形成三个相互独立的密 封盒， 并将相应工位的透明胶带的相应部位， 完全密闭在密封盒内， 从而 形成了三个相互独立的血样涂片处理工位， 其中 E与 E "构成了千燥工位 的干燥盒， F与 F〃构成了染色工位的染色盒， 0与6〃构成了干燥工作的 干燥盒。
本实施例的血涂片装置的工作过程是：
齿轮 （2 ) 通过透明胶带的齿孔， 带动透明胶带向前移动一个工位， 上隔离罩 （3) 与下隔离罩（4)合拢并密闭， 系统对处于 A工位透明胶带 部位进行血样的涂片操作，上隔离罩（3)与下隔离罩（4)分开，齿轮（2) 带动透明胶带向前移动一个工位，使透明胶带的血样涂片部位移到 B工位; 上隔离罩 （3)与下隔离罩（4)合拢并密闭， 透明胶带的血样涂片部 位处于 E与 E"构成的干燥盒内进行干燥处理， 同时系统对进入 A工位透 明胶带部位进行血样涂片的涂片操作， 上隔离罩（3)与下隔离罩（4)分 开， 齿轮 （2)通过透明胶带的齿孔， 带动透明胶带再向前移动一个工位， 使得透明胶带血样涂片干燥处理后的部位进入 C工位， 相应的透明胶带刚 作血样涂片操作的部位进入 B工位；
上隔离罩 （3) 与下隔离罩 （4) 再合拢并密闭， 在 F与 F"构成的密 闭的染色盒中， 系统对干燥处理后的透明胶带的血样涂片部位进行染色处 理， 在 E与 E〃构成的干燥盒内对进入 B工位的透明胶带的血样涂片部位 进行干燥处理，同时系统对进入 A工位透明胶带部位进行血样的涂片操作， 上隔离罩 （3) 与下隔离罩 （4) 分幵， 齿轮 （2)通过透明胶带的齿孔， 带动透明胶带再向前移动一个工位， 使得透明胶带血样涂片染色处理后的 部位进入 D工位， 干燥处理后的部位进入 C工位， 涂片操作后的部位进入 B工位；
上隔离罩 （3 ) 与下隔离罩 （4) 再次合拢并密闭， 系统在 G与 G"构 成的干燥盒内， 对透明胶带前次工序染色处理后的血样涂片部位进行干燥 处理， 在 F与 F〃构成的染色盒内， 对透明胶带前次工序干燥处理后的血 样涂片部位进行染色处理， 在 E与 E"构成的干燥盒内， 对透明胶带前次 工序涂片操作后的血样涂片部位进行干燥处理， 在 A工位， 对处于该工位 的透明胶带的部位进行涂片操作， 上隔离罩 （3) 与下隔离罩 （4) 再次分 开， 透明胶带再向前移动一个工位。
通过以上操作， 血涂片装置完成了一个工作序列， 也就是说， 血涂片 装置已经完成了在透明胶带上， 涂片、 干燥、 染色、 干燥的一个完整的过 程， 经涂片、 千燥、 染色、 干燥处理后的透明胶带的血涂片， 可以进入显 微观察、 计数等后续的操作。 而随着透明胶带按工作节拍的向前移动， 重 复以上所述的工作过程， 就实现了血涂片的自动化涂片操作。

Claims

1、 一种血涂片的基底， 其特征是基底为透明胶带。
2、 根据权利要求 1所述的基底， 其特征是透明胶带两边缘带有齿孔。
3、 一种权利要求 1所述的基底的血涂片装置， 包括血样涂片、 干燥、 染色、 干燥处理部件组成， 其特征是上隔离罩（3)和下隔离罩（4) 闭合 时， 由 E与 E"构成的干燥密封盒， F与 F' '构成染色密封盒， G与 G〃构 权
成干燥密封盒， 干燥密封盒设有热风进风孔和出气孔， 染色密封盒设有染 液注入孔和排出孔； 涂片、 干燥、 染色工序完成后， 上隔离罩 （3 ) 和下 隔离罩（4)分开， 传动机构带动透明胶带向前移动一个工位。 书
PCT/CN2007/002612 2006-09-06 2007-08-31 Substrat de frottis sanguin et dispositif de frottis sanguin WO2008040144A1 (fr)

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CN114112576A (zh) * 2021-11-22 2022-03-01 驻马店市中心医院 一种基于痰涂片抗酸染色的结核分枝杆菌耐药性检测系统

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CN105092342B (zh) * 2014-04-28 2019-10-11 深圳迈瑞生物医疗电子股份有限公司 血涂片染色方法以及血涂片染色盒
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