WO2007079938B1

WO2007079938B1 - Detergents or cleaning agents comprising a special amylase

Info

Publication number: WO2007079938B1
Application number: PCT/EP2006/012283
Authority: WO
Inventors: Cornelius Bessler; Arnd Kessler; Damien Drillon; Karl-Heinz Maurer; Susanne Wieland; Beatrix Kottwitz; Ingo Hardacker; Christian Nitsch; Johannes Zipfel; Maren Jekel
Original assignee: Henkel Kgaa; Cornelius Bessler; Arnd Kessler; Damien Drillon; Karl-Heinz Maurer; Susanne Wieland; Beatrix Kottwitz; Ingo Hardacker; Christian Nitsch; Johannes Zipfel; Maren Jekel
Priority date: 2005-12-28
Filing date: 2006-12-20
Publication date: 2008-02-28
Also published as: WO2007079938A2; WO2007079938A3; EP1974027A2; DE102006038448A1; EP2386632A1

Abstract

The invention relates to detergents or cleaning agents comprising a special α amylase which is characterized by characteristic sequence variations. The α amylase according to the invention is an α amylase variation that can be obtained from the starting α amylase that can be homologated with the α amylase AA560 by amino acid variations in the following positions: 9, 149, 182, 186, 202, 257, 295, 299, 323, 339 and 345 (as numbered according to α amylase AA560) or that can be obtained by certain multiple substitutions from α amylase AA560. Specific embodiments are characterized by α amylase variants with the following amino acid position assignments: 9L, 149A, 182T, 186A, 202L or 202I, 257I, 295F, 299Y, 323T, 339S and 345R and optionally also 118K, 183- (deletion), 184- (deletion), 195F, 320K and 458K (as numbered according to α amylase AA560). The invention also relates to agents comprising a natural α amylase with the above position assignments. Finally, the invention relates to corresponding washing or cleaning methods using said amylases and to possible uses thereof.

Claims

AMENDED CLAIMS received by the International Bureau on 21 December 2007 (21.12.2007)

1. washing or cleaning agent containing an α-amylase variant which can be obtained either from the α-amylase AA560 via amino acid changes in the following positions: 9, 149, 182, 186, 202, 257, 295, 299, 323, 339, 345 and optionally further (in the count according to the α-amylase AA560) or which can be obtained from the α-amylase AA560 via the following amino acid changes:

(1) M9L / M202I,

(2) M9L / M202I / M323T,

(3) M9L / M202I / M323T / M382Y,

(4) M9L / M202I / Y295F / A339S,

(5) M9L / M202I / Y295F,

(6) M9L / M202I / A339S,

(7) M9L / M202I / Y295F / A339S,

(8) M9L / M202I / Y295F / A339S / E345R,

(9) M9L / G149A / M202I / Y295F / A339S / E345R,

(10) M9L / M202L,

(11) M9L / M202L / M323T,

(12) M9L / M202L / M323T / M382Y,

(13) M9L / M202L / Y295F / A339S,

(14) M9L / M202L / Y295F,

(15) M9L / M202L / A339S,

(16) M9L / M202L / Y295F / A339S,

(17) M9L / M202L / Y295F / A339S, E345R,

(18) M9L / G149A / M202L / Y295F / A339S / E345R,

(19) M9L / M202T,

(20) M9L / M202T / M323T,

(21) M9L / M202T / M323T / M382Y,

(22) M9L / M202T / Y295F / A339S,

(23) M9L / M202T / Y295F,

(24) M9L / M202T / A339S,

(25) M9L / M202T / Y295F / A339S,

(26) M9L / M202T / Y295F / A339S / E345R,

(27) M9L / G149A / M202T / Y295F / A339S / E345R,

(28) M9L / G149A / M202I / V214T / Y295F / N299Y / M323T / A339S / E345R, 138

(29) M9L / G149A / M202L / V214I / Y295F / M323T / A339S / E345R / M382Y,

(30) M9L / G149A / G182T / G186A / M202I / V214I / Y295F / N299Y / M323T / A339S,

(31) M9L / G149A / G182T / G186A / M202L / T257I / Y295F / N299Y / M323T / A339S / E345R,

(32) M9L / G149A / M202L / V214T / Y295F / N299Y / M323T / A339S / E345R,

(33) M9L / G149A / M202I / V214I / Y295F / M323T / A339S / E345R / M382Y,

(34) M9L / G149A / G182T / G186A / M202L / V214I / Y295F / N299Y / M323T / A339S,

(35) M9L / G149A / G182T / G186A / M202I / T257I / Y295F / N299Y / M323T / A339S / E345R,

(36) M9L / G149A / M202I / V214T / Y295F / N299Y / M323T / A339S / E345R / N471 E,

(37) M9L / G149A / M202L / V214I / Y295F / M323T / A339S / E345R / M382Y / N471E,

(38) M9L / G149A / G182T / G186A / M202I / V214I / Y295F / N299Y / M323T / A339S / N471E,

(39) M9L / G149A / G182T / G186A / M202L / T257I / Y295F / N299Y / M323T / A339S / E345R / N471E,

(40) M202L / M105F / M208F,

(41) G133E / M202L / Q361E,

(42) G133E / M202L / R444E,

(43) M202L / Y295F,

(44) M202L / A339S,

(45) M202L / M323T,

(46) M202L / M323T / M309L,

(47) M202L / M323T / M430I,

(48) M202L / V214T / R444Y,

(49) M202L / N283D / Q361E,

(50) M202L / M382Y / K383R,

(51) M202L / K446R / N484Q,

(52) M202I / Y295F,

(53) M202I / A339S,

(54) M202I / M 105F / M208F,

(55) G133E / M202I / Q361E,

(56) G133E / M202I / R444E,

(57) M202I / M323T,

(58) M202I / M323T / M309L,

(59) M202I / M323T / M430I,

(60) M202I / V214T / R444Y,

(61) M202I / N283D / Q361E,

(62) M202I / M382Y / K383R, (63) M202I / K446R / N484Q,

(64) M202V / M 105F / M208F,

(65) G133E / M202V / Q361E,

(66) G133E / M202V / R444E,

(67) M202V / M323T,

(68) M202V / M323T / M309L,

(69) M202V / M323T / M430I,

(70) M202V / M323T / M9L,

(71) M202V / V214T / R444Y,

(72) M202V / N283D / Q361 E,

(73) M202V / M382Y / K383R,

(74) M202V / K446R / N484Q,

(75) M202T / M 105F / M208F,

(76) G133E / M202T / Q361 E,

(77) G133E / M202T / R444E,

(78) M202T / Y295F,

(79) M202T / A339S,

(80) M202T / M323T,

(81) M202T / M323T / M309L,

(82) M202T / M323T / M430I,

(83) M202T / M323T / M9L,

(84) M202T / V214T / R444Y,

(85) M202T / N283D / Q361 E,

(86) M202T / A339S,

(87) M202T / Y295F

(88) M202T / N299F.Y,

(89) M202T / M382Y / K383R or

(90) M202T / K446R / N484Q

2. Washing or cleaning agent according to claim 1, wherein the α-amylase variant is characterized by additionally one or more amino acid changes in the following positions relative to the α-amylase AA560: 118, 183, 184, 195, 320 and 458 (in the count according to the α-amylase AA560).

3. washing or cleaning agent according to claim 2, wherein the α-amylase variant has the following amino acid position assignments: 118K, 183- (deletion), 184- (deletion), 195F, 320K and / or 458K (in the count according to the α- Amylase AA560).

4. washing or cleaning agent according to any one of claims 1 to 3, wherein the α-amylase variant of the α-amylase AA560 or a derivative thereof can be derived and preferably derived from this itself.

A detergent or cleaner according to any one of claims 1 to 4, wherein said α-amylase variant in the fully-formulated agent has a concentration of the amylase enzyme protein of from 0.000001 to 5% by weight, and more preferably to 0.0000025 to 1% by weight, to 0.000005 to 0.5% by weight, to 0.0000075 to 0.1% by weight and most preferably to 0.000008 to 0.072% by weight, wherein furthermore, the concentration for automatic dishwashing agents very particularly preferably comprises 0.001 to 0.072% by weight and for textile detergents 0.0009 to 0.04% by weight.

6. Washing or cleaning agent according to one of claims 1 to 5, characterized in that it contains an α-amylase variant which has one of the following amino acid changes compared to the α-amylase AA560:

(10) M9L / M202L,

(28) M9L / G149A / M202I / V214T / Y295F / N299Y / M323T / A339S / E345R, (31) M9L / G149A / G182T / G186A / M202L / T257I / Y295F / N299Y / M323T / A339S I E345R,

(35) M9L / G149A / G182T / G186A / M202I / T257I / Y295F / N299Y / M323T / A339S I E345R,

(38) M9L / G149A / G182T / G186A / M202I / V214I / Y295F / N299Y / M323T / A339S I N471E,

(39) M9L / G149A / G182T / G186A / M202L / T257I / Y295F / N299Y / M323T / A339S I E345R / N471E,

(45) M202L / M323T,

M202L / M323T / M309L, (62) M202I / M382Y / K383R, (68) M202V / M323T / M309L, (73) M202V / M382Y / K383R (82) M202T / M323T / M430I (84) M202T / V214T / R444Y.

A detergent according to any one of claims 1 to 6, containing an anti-redeposition agent. 141

A detergent according to claim 7, wherein the antiredeposition agent is a cellulose or a cellulose derivative accessible by substitution and / or alkylation of hydroxyl groups, preferably carboxymethylcellulose (CMC).

9. washing or cleaning agent according to any one of claims 1 to 8, containing a bleaching agent or a bleach system of several components.

10. A washing or cleaning agent according to claim 9, wherein the bleaching agent or a constituent of the bleach system is an organic or inorganic per compound and / or an oxidative enzyme.

11. Washing or cleaning agent according to claim 10, wherein the bleaching agent or the constituent of the bleach system is percarbonate, perborate, phthalimidoperoxyhexanoic acid (PAP) and / or an oxidase, wherein in the latter case a substrate of this oxidase is preferably additionally present.

12. washing or cleaning agent according to any one of claims 1 to 11, wherein in addition bleach activators and / or bleach catalysts are included.

13. washing or cleaning agent according to claim 12, wherein the bleach activators and / or bleach catalysts are one or more of the following compounds: tetraacetylethylenediamine (TAED), Nonanoyloxybenzylsulfonat (NOBS), Lauryloxybenzylsulfonat (LOBS), iso-Lauryloxybenzylsulfonat (i LOBS), N-methylmorpholinum acetonitrile (MMA), pentaacetylglucose, a manganese-containing bleach catalyst, in particular 5,12-diethyl-1,5,8,12-tetraazabicyclo [6,6,2] hexadecane manganese dichloride (Mn-TACN), Cobalt-pentamine complexes, nitrile quats, benzoyl-caprolactam (BzCL), 4-nitrobenzoyl-caprolactam, 3-chlorobenzoyl-caprolactam, benzoyloxybenzylsulfonate (BOBS), phenylbenzoate (PhBz), decanoyloxybenzylsulfonate (C ₁₀ -OBS), benzoylvalerolactam (BZVL), octanoyloxybenzylsulfonate (C ₈ -OBS ), 4- [N- (nonanoyl) aminohexanoyloxy] benzylsulfonate sodium salt (NACA-OBS), 10-undecenoyloxybenzylsulfonate (UDOBS), decanoyloxybenzoic acid (DOBA), perhydrolysable esters, a perhydrolytic enzyme in Kombina tion with a hydrolyzable by this enzyme substrate.

14. A washing or cleaning agent according to any one of claims 1 to 13, wherein the bleaching agent or at least one component of the bleach system is provided in coated form, preferably as a coated tetraacetylethylenediamine (TAED). 142

15. Washing or cleaning agent according to one of claims 1 to 14, containing a protease, preferably a subtilisin protease.

16. A composition according to claim 15, wherein the protease in the fully-formulated agent in a concentration of the protease enzyme protein of 0.000001 to 5 wt .-% and increasingly preferably 0.0000025 to 1 wt .-%, to 0.000005 bis 0.5 wt .-%, at 0.0000075 to 0.15 wt .-% and most preferably at 0.00001 to 0.13 wt .-%, wherein still more particularly preferably the concentration for automatic dishwashing 0.003 to 0.13 wt .-% comprises.

17. Washing or cleaning agent according to claim 15 or 16, wherein the subtilisin protease is a wild-type protease or a protease variant.

18. Washing or cleaning agent according to claim 17, wherein the wild-type protease is one of the following:

The alkaline protease from Bacillus alcalophilus (DSM 11233),

the alkaline protease from Bacillus gibsonii (DSM 14391) or an at least 70% identical alkaline protease,

the alkaline protease from Bacillus sp. (DSM 14390) or at least 98.5% identical alkaline protease,

the alkaline protease from Bacillus sp. (DSM 14392) or an at least 98.1% identical alkaline protease,

the alkaline protease from Bacillus gibsonii (DSM 14393) or an at least 70% identical alkaline protease,

- in SEQ ID NO. 4 of the application WO 2005/063974 A1 described alkaline protease or an at least 40% identical alkaline protease,

- in SEQ ID NO. 4 of the application WO 2005/103244 A1 described alkaline protease or an at least 80% identical alkaline protease,

- in SEQ ID NO. 7 of the application WO 2005/103244 A1 described alkaline protease or a thereto at least 80% identical alkaline protease and

- in SEQ ID NO. 2 of the application DE 102005028295.4 described protease or a thereto at least 66% identical protease.

19. A washing or cleaning agent according to claim 18, wherein the protease variant is an amino acid change with respect to a starting protease homologizable with the Bacillus lentus alkaline protease in one or more of the following positions: 3, 4, 36 , 42, 43, 47, 56, 61, 69, 87, 96, 99, 101, 102, 104, 114, 118, 120, 143

130, 139, 141, 142, 154, 157, 188, 193, 199, 205, 211, 224, 229, 236, 237, 242, 243, 250, 253, 255 and 268 in the enumeration of Bacillus alkaline protease lentus.

The detergent or cleaner of claim 19, wherein the protease variant is one having an amino acid change from the parent molecule in one or more of the following positions: 3,4,43,61,188,193, 199,211 , 224, 250 and 253.

21. A washing or cleaning composition according to claim 20, which is one or more of the amino acid substitutions S3T, V4I, I43V, G61A, A188P, V193M, V199I, L211D, L211E, L211G, L211N or L211Q, A224V, S250G and S253N is.

22. A washing or cleaning agent according to any one of claims 19 to 21, wherein the starting molecule is the alkaline protease from Bacillus lentus, preferably from Bacillus lentus DSM 5483, or a variant thereof.

23. Washing or cleaning agent according to one of claims 1 to 22, containing a glucanase, which hydrolyzes plant polysaccharides, in particular a hemicellulase.

24. A washing or cleaning composition according to claim 23, wherein the glucanase in the fully-formulated agent in a concentration of the glucanase enzyme protein from 0.000001 to 5 wt .-%, and increasingly preferably 0.0000025 to 1 wt .-%, to 0 , 000005 to 0.5% by weight, to 0.0000075 to 0.1 wt .-% and most preferably to 0.00001 to 0.075% by weight, more preferably still the concentration for automatic dishwashing detergent 0.001 to 0.0072% by weight and for textile detergents 0.0009 to 0.04% by weight.

25. Washing or cleaning agent according to claim 23 or 24, wherein the glucanase or hemicellulase is a β-glucanase.

26. Washing or cleaning agent according to claim 23 or 24, wherein the glucanase or hemicellulase is a mannanase.

27. Washing or cleaning agent according to claim 23 or 24, wherein the glucanase or hemicellulase is a pullulanase.

28. Washing or cleaning agent according to one of claims 1 to 27, containing a cellulase. 144

29. A washing or cleaning composition according to claim 28, wherein the cellulase in the fully-formulated agent in a concentration of cellulase enzyme protein of 0.000001 to 5 wt .-%, and increasingly preferably 0.0000025 to 1 wt .-%, to 0 , 000005 to 0.5 wt .-%, to 0.0000075 to 0.1 wt .-% and most preferably to 0.00001 to 0.072 wt .-%, wherein still more preferably the concentration for laundry detergent 0 , 0007 to 0.005 wt .-% comprises.

30. A washing or cleaning composition according to claim 28 or 29, wherein the cellulase is a cellulase mixture.

31. A washing or cleaning composition according to claim 28 or 29, wherein the cellulase is a single component cellulase.

32. Washing or cleaning agent according to any one of claims 1 to 31, containing complexing agents and / or builders.

33. Washing or cleaning agent according to claim 32, wherein it contains a zeolite builder.

34. Washing or cleaning agent according to one of claims 1 to 33, containing an organic chemical polymer.

35. Washing or cleaning agent according to claim 34, wherein the organic chemical polymer is an anionic or cationic polymer.

36. The cleaning agent of claim 34 or 35, wherein the polymer is a rinse aid polymer.

37. Washing or cleaning agent according to one of claims 1 to 36, containing a nonionic surfactant.

38. The detergent of claim 37, wherein the nonionic surfactant is a hydroxy mixed ether.

39. Washing or cleaning agent according to one of claims 1 to 36, containing an optical brightener. 145

40. Washing or cleaning agent according to claim 39, wherein the optical brighteners are diphenyl compounds, in particular distyryl biphenyl derivatives, and / or stilbentriazine derivatives.

41. Washing or cleaning agent according to one of claims 1 to 40, characterized in that it contains one or more ingredients from the group of bleaching agents and bleach activators, complexing agents, phosphonates, polycarboxylates such as citrates and polymeric polycarboxylates, but also dispersants and strong salts, in particular Having sodium and / or calcium salts.

42. Washing or cleaning agent, in particular automatic dishwashing detergent according to one of claims 1 to 41, characterized in that it contains one or more nonionic surfactants having the general formula

R ^{1 is} -O- (CH ₂ -CH ₂ -O) _w - (CH ₂ -CH-O) _x - (CH ₂ -CH ₂ -O) _y - (CH ₂ -CH-O) ₂ -H,

I i

R ² R ³

contains, in which R ¹ is a C ₆ - R ² and R ³ w ₂₄ alkyl or alkenyl, which groups in each case for certain hydrocarbon radicals and the indices x, y, z are each integers to 6, or Surfactant system of at least one nonionic surfactant F of the general formula

R 1 is CH (OH) CH ₂ O- (AO) _W- (A ¹ O) _x - (A ¹ O) V- (A ¹¹ O) _Z -R ²

and at least one nonionic surfactant G of the general formula

R ¹ -O- (AO) _w - (A ^l O) _x - (A ^ll O) _y - (A "'O) _z -R ^2,

in which R ^{1 is} a C _6-24 -AikyI or alkenyl radical, R ^{2 is} a hydrocarbon radical having 2 to 26 carbon atoms, A, A ¹ , A "and A '" respectively for certain hydrocarbon radicals and w, x, y and z are each values up to 25, this surfactant system comprising surfactants F and G in a weight ratio between 1: 4 and 100: 1.

43. Washing or cleaning agent according to one of claims 1 to 42, characterized in that at least one oxidase and at least one perhydrolase.

44. Washing or cleaning agent according to claim 43, wherein the oxidase is selected from 146

a) choline oxidases whose amino acid sequence with the in SEQ ID NO. 2 specified

At least 76.5%, more preferably at least 78%, 80%, 82%, 84%, 86%, 88%, 90%, 92%, 94%, 95%, 96%, 97%, 98% of the amino acid sequence , 99% and more preferably 100%, b) choline oxidases whose amino acid sequence has the amino acid sequence shown in SEQ ID NO. 4 specified

At least 89%, more preferably at least 90%, 92%, 94%, 95%, 96%, 97%, 98%, 99% and particularly preferably 100% of the same amino acid sequence, c) choline oxidases whose amino acid sequence with the in SEQ ID NO. At least 83.8%, more preferably at least 84%, 86%, 88%, 90%, 92%, 94%, 95%, 96%, 97%, 98%, 99%, and particularly preferably, of the amino acid sequence indicated 100% match, d) choline oxidases whose amino acid sequence with the in SEQ ID NO. At least 76.4%, more preferably at least 78%, 80%, 82%, 84%, 86%, 88%, 90%, 92%, 94%, 95%, 96%, 97% 98%, 99% and particularly preferably 100%, and e) choline oxidases according to a), b), c) or d) obtained by single or multiple conservative amino acid substitution from a choline oxidase according to a) to d) or by derivatization , Fragmentation, deletion mutation or insertion mutation of a choline oxidase according to a) to d) are obtainable. and / or the perhydrolase is selected from f) perhydrolases whose amino acid sequence of SEQ ID NO. 26, but carries one or more amino acid substitutions at the sequence positions selected from 11, 15, 21, 38, 50, 54, 58, 77, 83, 89, 93, 96, 107, 117, 120, 134 , 135, 136, 140, 147, 150, 154, 155, 160, 161, 171, 179, 180, 181, 194, 205, 208, 213, 216, 217, 238, 239, 251, 253, 257, 261 , g) perhydrolases whose amino acid sequence of SEQ ID NO. 26, but carries one or more amino acid substitutions at the sequence positions selected from 11, 58, 77, 89, 96, 117, 120, 134, 135, 136, 140, 147, 150, 161, 208, 216 , 217, 238, h) perhydrolases whose amino acid sequence of SEQ ID NO. 26 indicated

But carries one or more amino acid substitutions at the sequence positions selected from 58, 89, 96, 117, 216, 217, i) perhydrolases whose amino acid sequence is as described in SEQ ID NO. 26 indicated

But corresponds to one or more of the amino acid substitutions T58A or T58Q, L89S, N96D, G117D, L216W and N217D, j) perhydrolases whose amino acid sequence has one of the amino acid sequence shown in SEQ ID NO. 8, 10, 12, 14, 16, 18, 20, 22 or 24 indicated amino acid sequences increasingly 147

preferably at least 70%, 72.5%, 75%, 77.5%, 80%, 82.5%, 85%, 87.5%, 90%, 92.5%, 95%, 97.5, respectively % and most preferably 100% matches.

45. washing or cleaning agent according to one of claims 1 to 44, characterized in that it is solid or at least has a solid phase containing the invention relevant amylase in optionally coated form, wherein the water content of the solid agent or the solid phase 1 to 20 wt .-%, preferably 3 to 18 wt .-% and in particular at most 15 wt .-% is.

46. Washing or cleaning agent according to one of claims 1 to 44, characterized in that it or a phase thereof which contains an amylase according to one of claims 1 to 6, has a water content of 50 to 99 wt .-%.

47. Washing or cleaning agent according to one of claims 1 to 46, characterized in that it or a phase thereof which contains an amylase according to one of claims 1 to 6, a viscosity of 50 mPas to 20,000 mPas, preferably from 50 mPas to 10000 mPas and in particular of at least 100 mPas or at most 8000 mPas.

48. Washing or cleaning agent according to one of claims 1 to 47, characterized in that it is liquid or contains at least one liquid phase containing an amylase relevant to the invention, and having at least one bleaching agent or a bleach system.

49. Washing or cleaning agent according to one of claims 1 to 48, characterized in that the washing or cleaning agent further contains phthalimidoperoxyhexanoic acid (PAP) and the α-amylase variant and the Phthalimidoperoxyhexansäure separated from each other in different phases of the washing or cleaning agent.

50. Washing or cleaning agent according to claim 49, characterized in that the washing or cleaning agent is in the form of a compressed, preferably in the form of a tableted molded article.

51. Washing or cleaning agent according to claim 50, characterized in that the detergent or detergent tablet has three, preferably four, preferably five and in particular six phases. 148

52. Washing or cleaning agent according to one of claims 49 to 51, characterized in that the phase which contains the α-amylase variant and the phase which contains the Phthalimidoperoxyhexansäure form no common phase boundary.

53. Washing or cleaning agent according to one of claims 49 to 52, characterized in that the washing or cleaning agent in the form of a water-soluble dosing unit, comprising the washing or cleaning agent and a water-soluble packaging material is present.

54. Washing or cleaning agent according to claim 53, characterized in that the water-soluble packaging material comprises a water-soluble polymer.

55. Washing or cleaning agent according to claim 54, characterized in that the water-soluble polymer has been at least partially processed molding by an injection molding process or a deep-drawing process.

56. Washing or cleaning agent according to one of claims 53 to 55, characterized in that the water-soluble dosing unit has two, preferably three, preferably four and in particular five phases.

57. washing or cleaning agent according to claim 49, characterized in that the washing or cleaning agent is packaged in a water-insoluble container having at least two separate chambers.

58. Washing or cleaning agent according to one of the preceding claims 49 to 57, characterized in that the washing or cleaning agent comprises at least one solid and at least one liquid phase.

59. Washing or cleaning agent according to one of the preceding claims 49 to 58, characterized in that the washing or cleaning agent comprises at least two solid and at least one liquid phase, wherein the α-amylase variant and the Phthalimidoperoxyhexansäure separated from each other in the solid phases while the liquid phase contains a nonionic surfactant.

60. Combination product comprising a packaging means and a detergent or cleaning agent according to one of claims 1 to 59, characterized in that the 149

Washing or cleaning agent comprises two separate liquid agents A and B in this packaging material and having the following composition: A: from 10 to 75% by weight of builder (s);

0.1 to 10% by weight of enzyme (s);

24.9 to 89.9% by weight of water; and B: 10 to 75% by weight of builder (s);

- 25 to 90 wt .-% water; wherein the liquid agent A has a pH (20 ⁰ C) between 6 and 9, while the liquid agent B has a pH (20 ⁰ C) between 9 and 14, none of the agents A or B more than 2 wt contains .-% of a bleaching agent and the α-amylase variant is included in the agent A.

61. Combination product comprising a packaging means and a washing or cleaning agent according to one of claims 1 to 59, characterized in that the washing or cleaning agent comprises two separate liquid agents A and B of the following composition located in this packaging means:

A: - 10 to 75% by weight of builder (s);

0.1 to 10% by weight of enzyme (s);

24.9 to 89.9% by weight of water; and B: 10 to 74.9% by weight of builder (s);

25 to 89.9% by weight of water;

- 0.1 to 15 wt .-% bleaching agent wherein the liquid agent A has a pH (20 ⁰ C) between 6 and 9 and the α-amylase variant is included in the agent A.

62. A washing or purification process in which an α-amylase variant comes into operation, which can be obtained from the α-amylase AA560 via amino acid changes in the following positions: 9, 149, 182, 186, 202, 257, 295, 299 , 323, 339, 345 and optionally further (in the count according to the α-amylase AA560) or which can be obtained from the α-amylase AA560 via the following amino acid changes:

(1) M9L / M202I,

(2) M9L / M202I / M323T,

(3) M9L / M202I / M323T / M382Y,

(4) M9L / M202I / Y295F / A339S,

(5) M9L / M202I / Y295F,

(6) M9L / M202I / A339S,

(7) M9L / M202I / Y295F / A339S, 150

(8) M9L / M202I / Y295F / A339S / E345R,

(9) M9L / G149A / M202I / Y295F / A339S / E345R,

(10) M9L / M202L,

(11) M9L / M202L / M323T,

(12) M9L / M202L / M323T / M382Y,

(13) M9L / M202L / Y295F / A339S,

(14) M9L / M202L / Y295F,

(15) M9L / M202L / A339S,

(16) M9L / M202L / Y295F / A339S,

(17) M9L / M202L / Y295F / A339S, E345R,

(18) M9L / G149A / M202L / Y295F / A339S / E345R,

(19) M9L / M202T,

(20) M9L / M202T / M323T,

(21) M9L / M202T / M323T / M382Y,

(22) M9L / M202T / Y295F / A339S,

(23) M9L / M202T / Y295F,

(24) M9L / M202T / A339S,

(25) M9L / M202T / Y295F / A339S,

(26) M9L / M202T / Y295F / A339S / E345R,

(27) M9L / G149A / M202T / Y295F / A339S / E345R,

(28) M9L / G149A / M202I / V214T / Y295F / N299Y / M323T / A339S / E345R,

(29) M9L / G149A / M202L / V214I / Y295F / M323T / A339S / E345R / M382Y,

(30) M9L / G149A / G182T / G186A / M202! / V214I / Y295F / N299Y / M323T / A339S,

(32) M9L / G149A / M202L / V214T / Y295F / N299Y / M323T / A339S / E345R,

(33) M9L / G149A / M202I / V214I / Y295F / M323T / A339S / E345R / M382Y,

(38) M9L / G149A / G182T / G186A / M202I / V214I / Y295F / N299Y / M323T / A339S / N471 E,

(39) M9L / G149A / G182T / G186A / M202L / T257I / Y295F / N299Y / M323T / A339S / E345R / N471 E,

(40) M202L / M105F / M208F,

(41) G133E / M202L / Q361 E, (42) G133E / M202L / R444E,

(43) M202L / Y295F,

(44) M202L / A339S,

(45) M202L / M323T,

(46) M202L / M323T / M309L,

(47) M202L / M323T / M430I,

(48) M202L / V214T / R444Y,

(49) M202L / N283D / Q361 E,

(50) M202L / M382Y / K383R,

(51) M202L / K446R / N484Q,

(52) M202I / Y295F,

(53) M202I / A339S,

(54) M202I / M105F / M208F,

(55) G133E / M202I / Q361E,

(56) G133E / M202I / R444E,

(57) M202I / M323T,

(58) M202I / M323T / M309L,

(59) M202I / M323T / M430I,

(60) M202I / V214T / R444Y,

(61) M202I / N283D / Q361E,

(62) M202I / M382Y / K383R,

(63) M202I / K446R / N484Q,

(64) M202V / M105F / M208F,

(65) G133E / M202V / Q361E,

(66) G133E / M202V / R444E,

(67) M202V / M323T,

(68) M202V / M323T / M309L,

(69) M202V / M323T / M430I,

(70) M202V / M323T / M9L,

(71) M202V / V214T / R444Y,

(72) M202V / N283D / Q361E,

(73) M202V / M382Y / K383R,

(74) M202V / K446R / N484Q,

(75) M202T / M 105F / M208F,

(76) G133E / M202T / Q361E,

(77) G133E / M202T / R444E,

(78) M202T / Y295F,

(79) M202T / A339S, 152

(80) M202T / M323T,

(81) M202T / M323T / M309L,

(82) M202T / M323T / M430I,

(83) M202T / M323T / M9L,

(84) M202T / V214T / R444 Y,

(85) M202T / N283D / Q361 E,

(86) M202T / A339S,

(87) M202T / Y295F

(88) M202T / N299F.Y,

(89) M202T / M382Y / K383R or

(90) M202T / K446R / N484Q

63. The washing or cleaning method according to claim 62, wherein the α-amylase variant is characterized by increasingly preferably additionally one or more amino acid changes in the following positions relative to the α-amylase AA560: 118, 183, 184, 195, 320 and 458 (in the count according to the α-amylase AA560).

64. The washing or cleaning method according to claim 63, wherein the α-amylase variant has the following amino acid position assignments: 118K, 183- (deletion), 184- (deletion), 195F, 320K and / or 458K (in the count according to the α- Amylase AA560).

65. A washing or cleaning process according to any one of claims 62 to 64, wherein the α-amylase can be derived from the α-amylase AA560 or a derivative thereof, and preferably has been derived from itself.

66. The washing or cleaning method according to any one of claims 62 to 65, wherein the α-amylase in a concentration of 0.000000005 to 0.25 g per I wash liquor and increasingly preferably from 0.0000000125 to 0.05, to 0.000000025 to 0.0025 wt .-%, to 0.0000000375 to 0.005 wt .-% and most preferably to 0.00000004 to 0.0036 g per I wash liquor comes into action, wherein still more preferably the concentration for automatic dishwashing 0 , 00001 to 0.0036 g per I wash liquor and for laundry detergents 0.0000045 to 0.0016 g per I wash liquor.

67. The washing or cleaning method according to any one of claims 62 to 66, wherein an α-amylase variant is used, which has one of the following amino acid changes compared to the α-amylase AA560: 153

(10) M9L / M202L,

(28) M9L / G149A / M202I / V214T / Y295F / N299Y / M323T / A339S / E345R, (31) M9L / G149A / G182T / G186A / M202L / T257I / Y295F / N299Y / M323T / A339S ι E345R,

(35) M9L / G149A / G182T / G186A / M202I / T257I / Y295F / N299Y / M323T / A339S ι E345R,

(45) M202L / M323T,

68. A washing or cleaning method according to any one of claims 62 to 67, characterized by one or more of the ingredient combinations specified in claims 7 to 44.

69. Washing or cleaning process according to one of claims 62 to 68, characterized by a comparatively short cleaning and / or washing time, wherein textile washing processes with washes with washing times of less than 40 min., Preferably less than 30 min., In particular of less than 20 min. are particularly preferred.

70. Washing or cleaning method according to one of claims 62 to 69, characterized by a comparatively low cleaning and / or washing temperature, including for automatic dishwashing detergent preferably 50 ° C or less, more preferably 40 ⁰ C or less and most preferably 30 ^0th C or less, or for textile detergent preferably 40 ⁰ C or less, more preferably 30 ⁰ C or less and most preferably 20 ⁰ C or less.

71. Use of an α-amylase variant for washing or purifying which can be obtained from the α-amylase AA560 via amino acid changes in the following positions: 9, 149, 154

182, 186, 202, 257, 295, 299, 323, 339, 345 and optionally further (in the count according to the α-amylase AA560), or which can be obtained from the α-amylase AA560 via the following amino acid changes:

(1) M9L / M202I,

(2) M9L / M202I / M323T,

(3) M9L / M202I / M323T / M382Y,

(4) M9L / M202I / Y295F / A339S,

(5) M9L / M202I / Y295F,

(6) M9L / M202I / A339S,

(7) M9L / M202I / Y295F / A339S,

(8) M9L / M202I / Y295F / A339S / E345R,

(9) M9L / G149A / M202I / Y295F / A339S / E345R,

(10) M9L / M202L,

(11) M9L / M202L / M323T,

(12) M9L / M202L / M323T / M382Y,

(13) M9L / M202L / Y295F / A339S,

(14) M9L / M202L / Y295F,

(15) M9L / M202L / A339S,

(16) M9L / M202L / Y295F / A339S,

(17) M9L / M202L / Y295F / A339S, E345R,

(18) M9L / G149A / M202L / Y295F / A339S / E345R,

(19) M9L / M202T,

(20) M9L / M202T / M323T,

(21) M9L / M202T / M323T / M382Y,

(22) M9L / M202T / Y295F / A339S,

(23) M9L / M202T / Y295F,

(24) M9L / M202T / A339S,

(25) M9L / M202T / Y295F / A339S,

(26) M9L / M202T / Y295F / A339S / E345R,

(27) M9L / G149A / M202T / Y295F / A339S / E345R,

(28) M9L / G149A / M202I / V214T / Y295F / N299Y / M323T / A339S / E345R,

(29) M9L / G149A / M202L / V214I / Y295F / M323T / A339S / E345R / M382Y,

(32) M9L / G149A / M202L / V214T / Y295F / N299Y / M323T / A339S / E345R,

(33) M9L / G149A / M202I / V214I / Y295F / M323T / A339S / E345R / M382Y,

(34) M9L / G149A / G182T / G186A / M202L / V214I / Y295F / N299Y / M323T / A339S, 155

(40) M202L / M105F / M208F,

(41) G133E / M202L / Q361E,

(42) G133E / M202L / R444E,

(43) M202L / Y295F,

(44) M202L / A339S,

(45) M202L / M323T,

(46) M202L / M323T / M309L,

(47) M202L / M323T / M430I,

(48) M202L / V214T / R444Y,

(49) M202L / N283D / Q361 E,

(50) M202L / M382Y / K383R,

(51) M202L / K446R / N484Q,

(52) M202I / Y295F,

(53) M202I / A339S,

(54) M202I / M105F / M208F,

(55) G133E / M202I / Q361E,

(56) G133E / M202I / R444E,

(57) M202I / M323T,

(58) M202I / M323T / M309L,

(59) M202I / M323T / M430I,

(60) M202I / V214T / R444Y,

(61) M202I / N283D / Q361E,

(62) M202I / M382Y / K383R,

(63) M202I / K446R / N484Q,

(64) M202V / M105F / M208F,

(65) G133E / M202V / Q361E,

(66) G133E / M202V / R444E,

(67) M202V / M323T,

(68) M202V / M323T / M309L,

(69) M202 V / M323T / M430I, 156

(70) M202V / M323T / M9L,

(71) M202V / V214T / R444Y,

(72) M202V / N283D / Q361 E,

(73) M202V / M382Y / K383R,

(74) M202V / K446R / N484Q,

(75) M202T / M 105F / M208F,

(76) G133E / M202T / Q361 E,

(77) G133E / M202T / R444E,

(78) M202T / Y295F,

(79) M202T / A339S,

(80) M202T / M323T,

(81) M202T / M323T / M309L,

(82) M202T / M323T / M430I,

(83) M202T / M323T / M9L,

(84) M202T / V214T / R444 Y,

(85) M202T / N283D / Q361 E,

(86) M202T / A339S,

(87) M202T / Y295F

(88) M202T / N299F.Y,

(89) M202T / M382Y / K383R or

(90) M202T / K446R / N484Q

72. Use according to claim 71, wherein further washing or cleaning-active substances come into effect.

73. Use according to claim 71 or 72, wherein the α-amylase variant is characterized by increasingly preferably additionally one or more amino acid changes in the following positions in relation to the α-amylase AA560: 118, 183, 184, 195, 320 and 458 (in US Pat Count according to α-amylase AA560).

74. Use according to any one of claims 71 to 73, wherein the α-amylase variant has the following amino acid position assignments: 118K, 183- (deletion), 184- (deletion), 195F, 320K and / or 458K (in the count according to the α Amylase AA560).

75. Use according to any one of claims 71 to 74, wherein the α-amylase can be derived from the α-amylase AA560 or a derivative thereof, and preferably has been derived from this itself. 157

76. Use according to any one of claims 71 to 75, wherein the α-amylase in a concentration of 0.000000005 to 0.25 g per I wash liquor and increasingly preferably from 0.0000000125 to 0.05, to 0.000000025 to 0, 0025 wt .-%, to 0.0000000375 to 0.005 wt .-% and most preferably to 0.00000004 to 0.0036 g per I wash liquor comes into action, wherein still more preferably the concentration for automatic dishwashing 0.00001 bis 0.0036 g per l wash liquor and for laundry detergents 0.0000045 to 0.0016 g per l wash liquor.

77. Use according to any one of claims 71 to 76, wherein the α-amylase has one of the following amino acid changes over the α-amylase AA560:

(10) M9L / M202L,

(28) M9L / G149A / M202I / V214T / Y295F / N299Y / M323T / A339S / E345R, (31) M9L / G149A / G182T / G186A / M202L / T257I / Y295F / N299Y / M323T / A339S / E345R,

(45) M202L / M323T,

78. Use of a composition according to any one of claims 1 to 6 for reduced odor formation on the washed or cleaned Good.

79. Use according to claim 78, characterized in that the α-amylase variant used in combination with one or more ingredients from the group of bleaching agents and bleach activators, complexing agents, phosphonates, polycarboxylates such as citrates and polymeric polycarboxylates, but also dispersants and strong salts becomes. 158

80. Use according to one of claims 71 to 79, characterized by a comparatively short cleaning and / or washing time, below for laundry detergents to a wash cycle of less than 40 min., Preferably less than 30 min., Particularly preferably less than 20 min ..

81. Use according to one of claims 71 to 80, characterized by a comparatively low cleaning and / or washing temperature, including for automatic dishwashing preferably 50 ⁰ C or less, more preferably 40 ⁰ C or less and most preferably 30 ⁰ C or less, or for the machine washing of textiles preferably 40 ⁰ C or less, more preferably 30 ⁰ C or less and most preferably 20 ⁰ C or less.