Classifications

• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
  • A61K31/19—Carboxylic acids, e.g. valproic acid
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01L—SHOEING OF ANIMALS
  • A01L15/00—Apparatus or use of substances for the care of hoofs
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
  • A61K8/36—Carboxylic acids; Salts or anhydrides thereof
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K9/00—Medicinal preparations characterised by special physical form
  • A61K9/08—Solutions
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P17/00—Drugs for dermatological disorders
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/02—Local antiseptics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/04—Antibacterial agents
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
  • A61Q19/00—Preparations for care of the skin
  • A61Q19/10—Washing or bathing preparations

Definitions

• the present invention relates to a hoof cleaning agent for prevention of cow's hoof disorders, and preparation and use thereof. More specifically, the present invention relates to a hoof cleaning agent for prevention of cow's hoof disorders, which is used as a raw material of a hoof cleaning solution of a hoof bath for cow's hooves and has combined functions of washing and sterilization, and use and preparation thereof.
• hoofed domestic animals such as Korean native cows (Hanwoo), dairy cows and the like may suffer from or may be susceptible to significant hoof disorders caused by inhabitation and growth of a variety of harmful bacteria and fungi in the open volume defined broadly by the hoof bottom wall. Due to a large body frame and heavy weight of Korean native cows and dairy cows, there would be the heavy weight load imposed on the hooves and legs thereof. Therefore, hoof disorders may be fatal to survival of cows which usually keep standing all day long.
• dairy farmers have taken a step to treat the animals by a method involving applying antibiotic ointments to the affected areas of the animal and then applying a bandage thereto, or a method of spraying a copper sulfate (CuSO 4 ) solution to the affected lesions, upon incidence of such diseases.
• the copper sulfate solution or a chemical substance such as formalin has also been employed as a hoof cleaning solution for use in a hoof bath or hoof-cleaning room.
• the present invention has been made in view of the above problems, and it is an aspect of the present invention to provide a hoof cleaning agent for prevention of cow's hoof disorders, and use and preparation thereof, which are capable of simultaneously achieving cleaning and sterilizing effects of cow's hooves, thus allowing for sterilization of affected animal hooves under cleaned conditions of the contaminated area and consequently resulting in early prevention of the productivity decrease such as a low milk yield and a low breeding coefficient via prophylaxis of cow's hoof disorders and avoidance of extreme situations such as culling, and are also capable of making great contributions to the promotion of cow's welfare.
• a hoof cleaning agent for prevention of cow's hoof disorders simultaneously contains cleaning components and sterilizing components. Therefore, when the hoof cleaning agent is diluted in water to form a hoof cleaning solution for use in a hoof bath and is then applied to hooves of the cow through the hoof bath, the cleaning components of the hoof cleaning solution first removes foreign materials adhered to interstices between cloven hooves of the animal while harmful bacteria adhered to the animal hooves are completely exposed to the hoof cleaning solution, and under such conditions, the adhered bacteria are thoroughly sterilized by sodium hypochlorite contained in the hoof cleaning solution.
• hoof cleaning solution utilizing the hoof cleaning agent of the present invention enables effective prevention and treatment of cow's hoof disorders, which in turn results in early prevention of productivity reduction such as a low milk yield and a low breeding coefficient and avoidance of potential extreme situations such as cow culling, as well as achievement of great contributions to the promotion of cow's health and comfort.
• a hoof cleaning agent for prevention of cow's hoof disorders comprising a wash liquid prepared by mixing a solid mixture of tartaric acid, citric acid, zinc sulfate, sodium molybdate and potassium nitrate with water, in order to remove foreign materials from cow's hooves, and a sodium hypochlorite for sterilizing harmful bacteria of cow's hooves from which foreign materials were removed.
• a hoof cleaning agent for prevention of cow's hoof disorders which is prepared by mixing solids of 15 to 25% by weight of tartaric acid, 15 to 25% by weight of citric acid, 15 to 25% by weight of zinc sulfate, 15 to 25% by weight of sodium molybdate and 15 to 25 % by weight of potassium nitrate; mixing and liquefying 50% by weight of the resulting mixture and 50% by weight of water to prepare a wash liquid; and mixing 50% by weight of the wash liquid and 50% by weight of sodium hypochlorite.
• a hoof cleaning agent as a hoof cleaning solution of a hoof bath for cow's hooves, wherein the hoof cleaning agent is diluted with water and a concentration of the hoof cleaning agent in the hoof cleaning solution is in a range of 5 to 16% by weight.
• a method of preparing a hoof cleaning agent for prevention of cow's hoof disorders comprising mixing solids of 15 to 25% by weight of tartaric acid, 15 to 25% by weight of citric acid, 15 to 25% by weight of zinc sulfate, 15 to 25% by weight of sodium molybdate and 15 to 25% by weight of potassium nitrate; mixing and liquefying 50% by weight of the mixture and 50% by weight of water to prepare a wash liquid; providing a stock solution of sodium hypochlorite; and mixing 50% by weight of the wash liquid and 50% by weight of the sodium hypochlorite stock solution.
• a hoof cleaning agent according to the present invention is used as a raw material which forms a hoof cleaning solution to be used in a hoof bath for cow's hooves, via dilution of the agent with water, and is provided by mixing a wash liquid, composed of a mixture of solids of 15 to 25% by weight of tartaric acid, 15 to 25% by weight of citric acid, 15 to 25% by weight of zinc sulfate, 15 to 25% by weight of sodium molybdate and 15 to 25% by weight of potassium nitrate and water, with a stock solution of sodium hypochlorite.
• the hoof cleaning agent is prepared by a process of mixing solids of 15 to 25% by weight of tartaric acid, 15 to 25% by weight of citric acid, 15 to 25% by weight of zinc sulfate, 15 to 25% by weight of sodium molybdate and 15 to 25% by weight of potassium nitrate; mixing and liquefying 50% by weight of the resulting mixture and 50% by weight of water to prepare a wash liquid; and mixing 50% by weight of the resulting wash liquid and 50% by weight of the sodium hypochlorite stock solution.
• the agent is preferably mixed again with water to form the hoof cleaning solution containing a 5% to 16% by weight of the agent (which will be demonstrated by the following experimental values hereinafter).
• the thus-prepared hoof cleaning solution is poured in a hoof bath through which cows are then allowed to pass, cow's hooves are dipped in the hoof cleaning solution during passage of the cows and bacteria inhabiting in cow's hooves are thoroughly removed by the washing action of the wash liquid and the sterilizing action of the sodium hypochlorite stock solution.
• tartaric acid, citric acid and zinc sulfate among components constituting the wash liquid are materials exerting excellent detergency, and a mixture thereof separates and removes foreign materials and contaminants adhered to cow's hooves during a short period of time at which cows pass through the hoof bath.
• sodium molybdate and potassium nitrate prevent deterioration in the intrinsic detergency of individual components which may occur upon intermixing of tartaric acid, citric acid and zinc sulfate.
• Table 1 shows a Minimal Inhibition Concentration (MIC) and a Minimal Bactericidal Concentration (MBC) of the hoof cleaning agent on isolated bacteria.
• test bacteria were grown in a broth tube made of transparent glass, containing Muller-Hinton broth, with addition of a commercial copper sulfate solution and dilute solutions thereof having different copper sulfate concentrations of 5.00%, 2.50%, 1.25%, 0.63%, 0.31%, 0.15% and 0.08%, respectively.
• the Minimal Inhibition Concentration (MIC) and Minimal Bactericidal Concentration (MBC) of copper sulfate solutions were determined.
• the MIC and MBC of the copper sulfate solution on Bacteria A and Bacteria B were 0.31%, respectively.
• the hoof cleaning solution of the present invention exhibited MBC of 5% for Bacteria A (E. coli). It was determined that the hoof cleaning solution shows a higher MBC as compared to the copper sulfate, but exhibits bactericidal effects on most species of bacteria isolated from cow's hooves, at a concentration of 5% or higher.
• Table 2 below shows the MIC and MBC of the hoof cleaning solution on Bacteria A (E. coli).
• the concentration of the hoof cleaning solution was set to 5.00%, 2.50%, 1.25%, 0.63%, 0.31%, 0.15% and 0.08%, respectively.
• the MIC and MBC of the hoof cleaning solution on E. coli were measured to be 1.25% and 5%, respectively, as shown in Table 2.
• CFU colony forming units
• a reduction in bacterial number exhibited a high rate of 95% or higher at the MBC concentration as well as at an in situ application concentration of the hoof cleaning agent for the hoof cleaning solution. That is, it was confirmed that the hoof cleaning solution containing the hoof cleaning agent in a concentration of 5% to 16% by weight exhibits a significant reduction of bacterial number.
• Table 4 shows the comparison results of bacterial number present in cloven hooves of cows, before and after treatment of animals with tap water, the copper sulfate solution and the hoof cleaning solution of the present invention, respectively.
• cows treated with the hoof cleaning solution of the present invention exhibited a significant reduction of the bacterial number from animal hooves, as compared to treatment of tap water or the copper sulfate solution.
• Table 5 below shows an incidence rate of hoof disorders in dairy cows which were raised in common cowshed.
• Table 6 shows a recovery rate of cows from a walking behavior index of 2-3 to a normal level (a value of 1), after treatment of animals with the copper sulfate solution and the hoof cleaning solution of the present invention, respectively.
• copper sulfate solution and the hoof cleaning solution used in this study copper sulfate and the hoof cleaning agent were respectively diluted to a concentration of 16% by weight.
• the copper sulfate solution and the hoof cleaning solution were replaced every 7 days.
• the tests were performed in 4 places (4 different cowsheds).
• Table 7 shows a recovery rate of cows from a walking behavior index of 4-5 to a normal level (a value of 1), after treatment of animals with the copper sulfate solution and the hoof cleaning solution of the present invention, respectively.
• copper sulfate solution and the hoof cleaning solution used in this study copper sulfate and the hoof cleaning agent were respectively diluted to a concentration of 16% by weight.
• the copper sulfate solution and the hoof cleaning solution were replaced every 7 days.
• Table 8 below shows an incidence rate of hoof disorders in dairy cows during application of the hoof cleaning solution.

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• Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Veterinary Medicine (AREA)
• Public Health (AREA)
• General Health & Medical Sciences (AREA)
• Animal Behavior & Ethology (AREA)
• Pharmacology & Pharmacy (AREA)
• Chemical & Material Sciences (AREA)
• Medicinal Chemistry (AREA)
• Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
• General Chemical & Material Sciences (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Organic Chemistry (AREA)
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• Communicable Diseases (AREA)
• Engineering & Computer Science (AREA)
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• Dermatology (AREA)
• Wood Science & Technology (AREA)
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• Detergent Compositions (AREA)
• Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
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• 2006
  • 2006-11-23 CN CN2006800427462A patent/CN101309677B/zh not_active Expired - Fee Related
  • 2006-11-23 KR KR1020087012344A patent/KR100997709B1/ko active IP Right Grant
  • 2006-11-23 WO PCT/KR2006/004950 patent/WO2007061236A1/en active Application Filing
  • 2006-11-23 JP JP2008539942A patent/JP4843680B2/ja active Active

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