WO2007048379A1 - Element de couplage optique pour cellule a ecoulement - Google Patents
Element de couplage optique pour cellule a ecoulement Download PDFInfo
- Publication number
- WO2007048379A1 WO2007048379A1 PCT/DE2006/001806 DE2006001806W WO2007048379A1 WO 2007048379 A1 WO2007048379 A1 WO 2007048379A1 DE 2006001806 W DE2006001806 W DE 2006001806W WO 2007048379 A1 WO2007048379 A1 WO 2007048379A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- coupling element
- optical coupling
- cell
- flow
- lens
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/0006—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00 with means to keep optical surfaces clean, e.g. by preventing or removing dirt, stains, contamination, condensation
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B13/00—Optical objectives specially designed for the purposes specified below
- G02B13/24—Optical objectives specially designed for the purposes specified below for reproducing or copying at short object distances
Definitions
- the invention relates to an optical coupling element which serves as a connecting piece between a flow cell and a microscopic evaluation device.
- An essential field of application of this optical coupling element is light and fluorescence microscopy for the examination of samples.
- these microorganisms or microbial aggregates are characterized microscopically in situ (Strathmann.M., Wingender.J., Flemming, H.-C, Application of fluorescently labeled lectins for the visualization and biochemical characterization of polysaccharides in Biofilms of Pseudomonas aeruginosa, Journal of Microbiological Methods 50, 237-248, 2002).
- the object of the invention is to develop an optical coupling element for the microscopic characterization of samples in flow cells in order to avoid a direct contact of the lens with the flow solution and thereby to keep the losses in the numerical aperture of the lens as low as possible.
- the invention replaceable optical coupling element leads to a protection of the lens. Due to the optical coupling element, an additional image plane is laid at the location of the original object plane so as not to have to accept any aperture deterioration. Another possible use of this coupling element is given in the coupling of cryogenic cells in the microscopic beam path.
- the sample carrier 2 is provided with a micrometer screw 10, via which the table can be moved freely in the Z direction.
- the optical coupling element is not designed to be interchangeable.
- an additional opening 12 is provided in the cell 1, via which the desired gas atmosphere is introduced into the cell 1.
- the gas is pressed through a filter 13 into the cell 1.
- An advantageous embodiment of the invention is to separate the second converging lens 8 by a transmitter liquid 14 from the nutrient liquid or the markers 15 in order to avoid contamination of the surface of the second converging lens 8.
- This transmitter liquid 14 is introduced if necessary via a channel 9 in the lid 5.
- a transmitter fluid 14 are suitable polar liquids that do not dissolve in water and form a meniscus 16 with water-like solutions due to the resulting surface tension.
- Fig. 2 shows the optical path between the objective 11 and the sample 3.
- a total of 3 (or 4 when using a transmitter liquid 14, [s Fig. 3]) additional optical active surfaces are inserted to the object (the sample 3) in to image the image plane 18 of the microscope. These are the two lenses and the spaces between them. Between the lens 7 and the lens 11, an additional image plane 19 is generated by the optical coupling element 7,8.
- Other optically active surfaces can be introduced if color correction is necessary.
- FIG. 3 shows the formation of a meniscus 16 between the polar transmitter liquid 14 and the bipolar nutrient medium 15.
Abstract
L'objectif de l'invention est de mettre au point un élément de couplage optique pour la caractérisation microscopique d'échantillons dans des cellules à écoulement, cet élément permettant d'éviter un contact direct de l'objectif avec la solution d'écoulement et de réduire ainsi au maximum les pertes au niveau de l'ouverture numérique de l'objectif. L'élément de couplage optique selon l'invention comprend une cellule (1), un ou plusieurs porte-échantillons (2) situés à l'intérieur de cette cellule (1), des amenées et des évacuations (4) pour garantir un flux continu de différentes solutions à travers la cellule (1), ainsi que des ouvertures (12) pour introduire une atmosphère de gaz inerte. L'invention se caractérise en ce qu'un évidement est ménagé dans la partie supérieure (5) pour l'examen microscopique des échantillons (3) in situ, en ce qu'un élément de couplage optique additionnel (7, 8) est placé dans l'évidement de la partie supérieure (5) et en ce que le système optique est constitué de deux milieux optiquement actifs qui sont disposés à l'opposé optiquement l'un vis-à-vis de l'autre.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102005051406A DE102005051406A1 (de) | 2005-10-27 | 2005-10-27 | Optisches Koppelelement für eine Durchflusszelle |
DE102005051406.5 | 2005-10-27 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2007048379A1 true WO2007048379A1 (fr) | 2007-05-03 |
Family
ID=37733712
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/DE2006/001806 WO2007048379A1 (fr) | 2005-10-27 | 2006-10-19 | Element de couplage optique pour cellule a ecoulement |
Country Status (2)
Country | Link |
---|---|
DE (1) | DE102005051406A1 (fr) |
WO (1) | WO2007048379A1 (fr) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4734372A (en) * | 1983-02-04 | 1988-03-29 | Brown University Research Foundation | Cell culturing methods and apparatus |
DE9101082U1 (fr) * | 1990-02-05 | 1991-04-18 | Kempe, Eberhard, Dipl.-Ing., 1000 Berlin, De | |
DE4032002A1 (de) * | 1989-10-11 | 1991-06-06 | Abb Patent Gmbh | In situ mikroskopsonde und messverfahren |
DE4443902C1 (de) * | 1994-12-01 | 1996-04-18 | Will Prof Dr Minuth | Kammer zur Kultivierung von Zellen, insbesondere Mikroskopkammer |
WO1999019752A1 (fr) * | 1997-10-09 | 1999-04-22 | Imagyn Medical Technologies, Inc. | Systeme d'objectif au saphir |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DD51714A1 (de) * | 1965-10-16 | 1966-12-05 | Annelies Heymer | Vorrichtung zur mikroskopischen Untersuchung unter hohem Druck bei tiefen Temperaturen |
GB9314926D0 (en) * | 1993-07-19 | 1993-09-01 | Optiglass Ltd | Spectrophotometer cell |
AT407012B (de) * | 1999-04-29 | 2000-11-27 | Werner Dipl Ing Dr Tech Juette | Verfahren zur behandlung von oberflächen sowie vorrichtung zur durchführung dieses verfahrens |
-
2005
- 2005-10-27 DE DE102005051406A patent/DE102005051406A1/de not_active Withdrawn
-
2006
- 2006-10-19 WO PCT/DE2006/001806 patent/WO2007048379A1/fr active Application Filing
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4734372A (en) * | 1983-02-04 | 1988-03-29 | Brown University Research Foundation | Cell culturing methods and apparatus |
DE4032002A1 (de) * | 1989-10-11 | 1991-06-06 | Abb Patent Gmbh | In situ mikroskopsonde und messverfahren |
DE9101082U1 (fr) * | 1990-02-05 | 1991-04-18 | Kempe, Eberhard, Dipl.-Ing., 1000 Berlin, De | |
DE4443902C1 (de) * | 1994-12-01 | 1996-04-18 | Will Prof Dr Minuth | Kammer zur Kultivierung von Zellen, insbesondere Mikroskopkammer |
WO1999019752A1 (fr) * | 1997-10-09 | 1999-04-22 | Imagyn Medical Technologies, Inc. | Systeme d'objectif au saphir |
Non-Patent Citations (1)
Title |
---|
HASHIZUME K ET AL: "Individual mesoscopic structures studied with sub-micrometer optical detection techniques: CdSe nanocrystals capped with TOPO and ZnS-overcoated system", JOURNAL OF LUMINESCENCE, AMSTERDAM, NL, vol. 98, no. 1-4, July 2002 (2002-07-01), pages 49 - 56, XP004368922, ISSN: 0022-2313 * |
Also Published As
Publication number | Publication date |
---|---|
DE102005051406A1 (de) | 2007-05-03 |
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DPE2 | Request for preliminary examination filed before expiration of 19th month from priority date (pct application filed from 20040101) | ||
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
122 | Ep: pct application non-entry in european phase |
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