WO2006061877A1

WO2006061877A1 - Propolis extract with improved water dispersibility, propolis-containing beverage, and method of improving water dispersibility of propolis extract

Info

Publication number: WO2006061877A1
Application number: PCT/JP2004/018163
Authority: WO
Inventors: Rindai Yamamoto
Original assignee: Rindai Yamamoto
Priority date: 2004-12-06
Filing date: 2004-12-06
Publication date: 2006-06-15
Also published as: TW200621169A; CN101083915A; CN101083915B

Abstract

[PROBLEMS] To provide a method of relieving the adhesion to container, inside of oral cavity and throat and difficulty in flavor enhancement being drawbacks of propolis with excellent performance without the use of substances having the danger of hindering the absorption of milk protein, etc., and to provide a propolis extract with improved water dispersibility wherein the above drawbacks have been relieved. [MEANS FOR SOLVING PROBLEMS] There is provided a propolis extract with improved water dispersibility, produced by heating a propolis extract obtained by alcohol extraction in the presence of sodium carbonate or/and sodium hydrogen carbonate.

Description

Specification

Water dispersibility improved propolis extract, propolis-containing beverage, and method for improving water dispersibility of propolis extract

Technical field

[0001] The present invention relates to a propolis extract with improved dispersibility in water and a beverage containing such a propolis extract.

Background art

[0002] Due to the recent health boom and naturalism orientation, various natural health foods are attracting attention. Among these natural health foods, the natural antibiotics produced by bees or the propolis extract from which propolis power, also called natural cell-active substances, has been extracted are intended to promote health and improve health. Or, it is particularly attracting attention as a high anti-cancer effect.

[0003] Such a propolis extract is supplied in a dropper bottle or the like in which a dropper is set in a cap as a liquid (propolis liquid) that can be mixed with ethanol or other suitable solvent and dropped with a dropper. For health maintenance purposes, 20-30 drops of the liquid is dropped into a suitable amount of water, hot water, milk, coffee, tea, juice, honey, drinks or alcoholic beverages, in the morning, noon and evening. Ingested orally three times a day, or in the morning, noon, candy, and 4 times a day before going to bed four times a day before going to bed, it is said to be taken orally by dropping into the beverage.

[0004] However, when the propolis solution dissolved in ethanol is dropped into the beverage as described above, the water-insoluble component of the propolis extract is dissolved in the beverage. It may become “swift” and float on the surface of the beverage, or may sink and adhere to the bottom or side of the container, making it impossible to take the required amount.

[0005] Of these, ingredients that float on the surface of the beverage may stick to the drinker's teeth, gums, tongue, palate, or throat during drinking and may not be able to be effectively consumed, and even after drinking For some time, discomfort or irritation continues.

[0006] On the other hand, the components adhering to the bottom and side surfaces of the container cannot be drunk naturally, and thus are effectively utilized. In addition, it cannot be removed easily by ordinary cleaning methods (cleaning with a detergent) (especially if it adheres to lacquer ware, it is impossible to wash and remove without damaging the container. There was a problem that said.

[0007] Furthermore, the propolis extract is so-called “bitter taste” in terms of taste, and feels “spicy” at the time of quoting, and it is generally difficult to drink because it smells too strong. Here, it is also possible to use means such as capsules. The amount of a single dose is 20-30 drops! / 50-50 and so many drops! It was suitable for drinking! /, Became large, or increased in the number of capsules to be consumed at one time.

[0008] Therefore, studies have been made to mix propolis liquid with other beverage components to make it easier to drink, but the above-mentioned problem of adhesion to containers has not been solved and does not adhere. When it is blended to the extent, the blending amount is reduced, and the necessary dose for securing a sufficient dose of propolis extract is increased to, for example, 200 mL. At this time, since the amount is large, breathing is necessary while drinking, but if you breathe, you will be reluctant to continue drinking afterwards, or you may not be able to drink the necessary amount, or you may drink all of the amount. However, it was difficult to improve the flavor and make it easier to drink.

Here, in order to improve the water dispersibility of the propolis extract, Japanese Patent Laid-Open No. 2001-169736 (Patent Document 1) proposes a technique of adding and blending milk proteins such as milk casein.

[0010] However, the above-mentioned drawback that the amount of liquid required for sufficient dispersion is considerably large and therefore the amount necessary for securing a sufficient amount of the propolis extract is increased cannot be solved. . Also, for example, it is known that when a child accidentally swallows a detergent etc., milk protein is used as the first treatment to prevent the absorption of other substances in milk protein. It has the function of delaying or preventing absorption, and it has been pointed out that the above-mentioned conventional technology cannot incorporate the active ingredient of Provolis into the body.

Patent Document 1: JP 2001-169736 A

Disclosure of the invention

Problems to be solved by the invention [0011] The present invention improves the above-mentioned conventional problems, that is, the disadvantages of propolis having excellent characteristics without using milk protein or the like, adhesion to containers, oral cavity and throat, flavor The object is to provide a method for improving the difficulty of improvement and a propolis in which these drawbacks are improved.

Means for solving the problem

[0012] In order to solve the above-mentioned problem, the water dispersibility-improved propolis extract of the present invention is a propolis extract by alcohol extraction as described in claim 1, and is sodium carbonate, Z, or hydrogen carbonate. Water-dispersible modified propolis extract heat-treated in the presence of sodium.

[0013] The propolis-containing beverage of the present invention is a propolis-containing beverage containing the water dispersibility-improved propolis extract as described above.

[0014] Further, the method for improving water dispersibility of a propolis extract of the present invention is a propolis extract obtained by alcohol extraction, wherein the propolis extract has a constitution in which heat treatment is performed in the presence of sodium carbonate or Z and sodium bicarbonate. This is a method for improving the water dispersibility of products. The invention's effect

[0015] According to the water dispersibility-improved propolis extract of the present invention, since there is no attachment to the container, the oral cavity or the throat, there is no wasteful part attached to the container, so that the entire amount can be ingested. The container can be easily cleaned after use, and it can be used repeatedly, and it is not necessary to carry a dedicated container when going out, and the discomfort caused by attachment to the oral throat, An unpleasant taste with no unpleasantness and a long-lasting odor is prevented.

[0016] Furthermore, since it can be easily dispersed in water and no sedimentation is observed for several weeks, it can be easily processed into a propolis-containing beverage having a flavor that is easy to drink. A beverage containing a propolis extract that is easy to clean, and can contain only a small amount of beverage that can be drunk in a single or half mouth, but can contain the necessary amount of propolis for a healthy person It can be.

Brief Description of Drawings

[0017] FIG. 1 is a chromatogram obtained by high-performance liquid chromatography of a propolis solution before treatment for improving water dispersibility. [Fig. 2] High-performance liquid chromatogram of propolis extract (A) with improved water dispersibility.

BEST MODE FOR CARRYING OUT THE INVENTION

[0018] A propolis extract obtained by alcohol extraction is obtained in the form of a propolis solution dissolved in ethanol. The content of the propolis extract in this propolis liquid is generally 20-33% by volume, and in addition, some water (about 10-15% by volume) and ethanol (remaining amount).

In the present invention, the propolis extract contained in such a propolis liquid is subjected to a heat treatment in the presence of sodium carbonate or Z and sodium bicarbonate.

[0020] The amount of sodium hydrogen carbonate and the sodium carbonate water dispersibility improver varies depending on the content of the propolis extract contained in the pour-polis solution to be used. In the case of 33% by volume of propolis liquid, it is 0.5% by mass or more and 2% by mass or less. At this time, if the addition amount is less than 0.5% by mass, sufficient water dispersibility improvement effect may not be obtained. Moreover, if the added amount is close to 2% by mass, a sufficient effect of improving water dispersibility can be obtained, and even if added in excess of 2% by mass, it does not dissolve in the propolis solution, and it appears to be unreacted even after heat treatment. In some cases, it may be necessary to remove the powder by means such as filtration. In the case of a propolis liquid having a different volume content of the propolis extract, the water dispersibility improver should be converted so that it falls within the range of the ratio of the propolis extract and the water dispersibility improver in the above example. Added.

[0021] In the method for producing a water dispersibility-improving propolis extract of the present invention, it is necessary to use sodium hydrogen carbonate or sodium carbonate as the water dispersibility improver. Even if edible acid such as citrate is used or heat treatment is performed after sodium hydroxide is added so that the propolis solution becomes neutral, sufficient water dispersibility improvement effect can be obtained. Nah ...

[0022] In this way, after adding sodium carbonate, certain! /, Z and sodium hydrogen carbonate to the propolis solution, heat treatment is performed.

A preferable heat treatment temperature has a lower limit of 40 ° C. or higher, desirably 50 ° C. or higher, and an upper limit of 70 ° C. or lower, desirably 65 ° C. or lower.

[0024] The heat treatment continues until foaming by heating is completed (usually about 15-30 minutes). When the force is 40 ° C or less, foaming does not end even if the heat treatment is performed for more than 30 minutes. At this time, A sufficient water dispersibility improvement effect may not be obtained. On the other hand, if the temperature exceeds 70 ° C, the treated propolis solution may turn red, and “pungency” or irritation to the throat during drinking may increase.

[0025] As described above, is a propolis extract obtained by alcohol extraction, and is the water dispersibility-improved propolis extract heat-treated in the presence of sodium carbonate, Z, or sodium bicarbonate uniformly emulsified? However, it does not settle even after several weeks. Furthermore, this water dispersibility-improved propolis extract has a high ethanol content, so if it is sealed, there is no risk of spoilage.

[0026] In addition, the content of each major component of quercetin, kaempferol, diterpene, and paracoumaric acid, which is considered to be an active ingredient in the propolis extract, does not change before and after the treatment. There is no difference in the components or the results of high-performance liquid chromatography. In addition, no change was seen before and after the water dispersibility improvement treatment in the analysis using an absorptiometer in the ultraviolet region.

[0027] The water dispersibility-improved propolis extract thus obtained is excellent in water dispersibility, so that the components settle even when mixed with general soft drinks, tea, tea, etc. at various mixing ratios. There is nothing to do.

[0028] Therefore, a propolis-containing beverage can be easily obtained by blending the water-dispersibility-improved propolis extract in the production process of these soft drinks, etc., so that masking of the odor peculiar to propolis becomes easier. Easy to drink or drink.

Example

[0029] Examples of the water dispersibility-improved propolis extract of the present invention will be specifically described below.

[0030] <Examination of effects of processing conditions>

The propolis solution is an ethanol solution containing 32% by volume propolis extract (however,

, Containing some water).

[0031] To these propolis solutions, sodium bicarbonate (NaHCO3) in the amounts shown in Tables 1 and 2 was used.

) Or sodium carbonate (Na 2 CO 3) and then heating at the temperatures shown in Table 1

3 2 3

The treatment was carried out until foaming was completed (about 15-25 minutes). [0032] At this time, whether or not a film has formed on the liquid level in the container, and whether or not there are deposits on the wall surface and bottom surface of the original container when transferred to another container are visually observed. In addition to the investigation, it was actually consumed and a sensory test was conducted. The results are shown in Table 1.

[0033] [Table 1]

[0034] [Table 2]

Amount added (mass ¾) Heat treatment Membrane container Special instructions for visual inspection and sensory test

NaHCO Na ₂ C0 ₃ temperature (° C) formation adhesion

0 3. 0 4 5 None Slightly powdered precipitate, completely dispersed after filtration removed, evenly dispersed

0 1 .0 4 5 None

Yes

0 1 .5 5 0 None

Yes

0 1 .5 6 0 None None Completely uniform dispersion

0 3. 0 7 0 None None Precipitate in powder form, completely uniform dispersion after removal by filtration, discoloration (red), strong irritation to throat when drinking after filtration

1.0 1, 5 6 0 None None Completely uniform dispersion

[0035] According to Tables 1 and 2, in the system in which the total amount of sodium hydrogen carbonate and sodium carbonate added to the propolis liquid was 2% by mass or more, a powdery precipitate that was thought to be a residue was observed. It can be seen that the dispersibility was improved in the system with a total addition amount of 2% by mass or less. In Tables 1 and 2 above, samples that were uniformly dispersed were subjected to a standing test at room temperature for 3 weeks under sealed conditions, but the uniform dispersion state was maintained with no precipitation.

[0036] The pH of the water dispersibility-improved propolis extract in Tables 1 and 2 is about 6.7-6.9 compared to 5.3 for the original propolis solution. It was almost neutral. In addition, the power of adding sodium hydroxide aqueous solution to untreated propolis solution to pH 6.8, followed by heat treatment at 60 ° C for 25 minutes. There was no improvement.

[0037] Among these water dispersibility-improved propolis extracts, the samples that were not attached to the container gave a residual feeling of adhesion to the lips and gums even during the actual sensory test. In addition, it was confirmed that there was almost no sticking to these samples even when the amount of adhesion to the container was very small. In both cases, it often occurred when untreated propolis solution was used. It was confirmed that the irritation to the throat was resolved within 5 minutes.

[0038] <Influence of Propolis on Water Dispersibility Improvement Treatment!> Here, with regard to the water dispersibility improved propolis extract (A) added with 2% by weight of sodium hydrogencarbonate and heat-treated at 60 ° C, the presence or absence of changes in its components before and after the water dispersibility improvement treatment Were examined by high performance liquid chromatography.

[0039] The analysis was performed using HPLC Class Lc 10 manufactured by Shimadzu Corporation as the instrument, Shimadzu Techno Research Co., Ltd. ODS-II (4.6 mm φ, 150 mm) as the column, and methanol, water vinegar and acetic acid mixed solution (volumes respectively) as the mobile phase. The ratio was 70, 30 and 1), and a photodiode array detector was used as the detector, the flow rate was 1.2 mLZ, the column temperature was 50 ° C, and the measurement wavelength was 275 ηm.

[0040] Fig. 1 shows the chromatogram of the propolis solution before the water dispersibility improvement treatment, and Fig. 2 shows the chromatogram of the water dispersibility improved provolis extract (A).

[0041] Furthermore, the water dispersibility-improved propolis extract (A) was evaluated for the degree of adhesion to the container at the same time as the evaluation with the propolis liquid without the water dispersibility improvement treatment.

[0042] In Fig. 1 and Fig. 2, the force that the peak appearing at the retention time of about 3 minutes (2. 95-2. 97 minutes) is paracoumaric acid, and other components, even water dispersibility It can be seen that there is no change before and after the improvement treatment and that no new components are generated by the water dispersibility improvement treatment.

[0043] Kenferrol and quercetin were measured under the above conditions, except that the detection wavelength was 380 nm. As a result, these components having peaks at retention times of about 25 minutes and about 52 minutes, and those components that do not change in peak height and area before and after the treatment, are also subjected to the water dispersibility improvement treatment of the present invention. It was confirmed that it was not affected.

[0044] <Examination of adhesion to drinking containers>

LOOmL of tap water was put in 12 transparent plastic cups whose masses were measured in advance, and 6 of these were pre-polish solution (propolis component content 32% by mass: 0.48) before water dispersibility improvement treatment. In the other six, the above-described water dispersibility-improved propolis extract A (propolis component content 32%: 0.48 g (the added sodium hydrogen carbonate was reacted in its entirety, and the added sodium hydrogen carbonate-derived sodium component mass was 1.5 g (about 60 drops, equivalent to the daily dose that is considered sufficient for maintaining health) was added dropwise. [0045] After these containers were shaken lightly and stirred, the liquid contents were discarded, and then the containers were dried by natural drying. Then, the change in mass of each container was examined, and the amount of adhered substances was measured and averaged.

As a result, in the case of the propolis liquid before the water dispersibility improving treatment, the average mass of the adhered residue is 0.1 l lg, and this amount corresponds to a little over 7% as the propolis component.

[0047] On the other hand, in the case of propolis extract A with improved water dispersibility according to the present invention, the average amount of adhering residue in the cup is 0.002 g, indicating that there is virtually no adhering residue. confirmed. It was confirmed that the slight adhesion residue at this time can be easily removed by hand washing with running water.

[0048] From these results, it can be seen that the water dispersibility-improved propolis extract according to the present invention does not adhere to the container, so that the entire amount can be surely consumed.

[0049] In addition, it was confirmed that the water dispersibility-improved propolis extract A does not impair its good water dispersibility even when dripped not only in water but also in green tea, black tea, and the like. In addition, because of its good water dispersibility, it was also confirmed that the components do not sink even if the residual alcohol content is reduced by, for example, decompression or heating after addition to beverages or water. It has been found that the water dispersibility-improved provolis extract of the present invention can also be applied to the field of non-alcoholic beverages and the like in which the above problems are problematic.

Industrial applicability

[0050] According to the method for improving the water dispersibility of a propolis liquid according to the present invention, the disadvantages of a propolis having a number of excellent properties are the disadvantages of adhesion to containers, oral cavity and throat, and difficulty in improving flavor. It can be remarkably improved without using milk protein or the like. For this reason, the water dispersibility-improved propolis extract according to the present invention can be easily applied to the food and beverage fields, which have been difficult to apply, as well as the fields where the conventional propolis extract is used.

Claims

The scope of the claims

[1] A propolis extract obtained by alcohol extraction, which is heat-dispersed in the presence of sodium carbonate or Z and sodium bicarbonate, and is improved in water dispersibility.

[2] The water dispersibility-improved propolis extract according to claim 1, wherein the heat treatment power is 0 ° C or higher and 70 ° C or lower.

[3] A propolis-containing beverage comprising the water-dispersibility-improved propolis extract according to claim 1 or 2.

[4] A method for improving water dispersibility of a propolis extract, which is a propolis extract obtained by alcohol extraction, wherein the extract is heated in the presence of sodium carbonate or Z and sodium bicarbonate.

[5] The method for improving water dispersibility of a propolis extract according to claim 2, wherein the heat treatment is performed at a temperature of 0 ° C or higher and lower than 70 ° C.