CN114306390A - Preparation method of bee therapy product for reducing blood sugar - Google Patents
Preparation method of bee therapy product for reducing blood sugar Download PDFInfo
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- CN114306390A CN114306390A CN202210075821.6A CN202210075821A CN114306390A CN 114306390 A CN114306390 A CN 114306390A CN 202210075821 A CN202210075821 A CN 202210075821A CN 114306390 A CN114306390 A CN 114306390A
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- propolis
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Abstract
The invention discloses a preparation method of a bee therapy product for reducing blood sugar, which comprises the following steps: the preparation method comprises the following steps: freezing propolis at-40 deg.C to-80 deg.C
Pulverizing into fine powder, and sieving with 80-100 mesh sieve; adding pulverized propolis into the powder
The volume percentage concentration of the times is
Adding surfactant into the ethanol solution, extracting with ultrahigh pressure extraction equipment under 30T600Mpa for heating treatment in the presence of sodium carbonate and sodium bicarbonate
Description
Technical Field
The invention relates to the technical field of bee therapy products, in particular to a preparation method of a bee therapy product for reducing blood sugar.
Background
Propolis is a viscous substance prepared by collecting vegetable resin from western bees, mixing with beeswax, pollen and gland secretion of bees, and chewing with bees, and has pleasant fragrance. The bee uses propolis to fill the gap of the honeycomb, smoothes the inner wall of the honeycomb, and prevents the rotting of foreign matters and the infection of germs in the honeycomb by the propolis. At present, propolis products at home and abroad are basically extracted by alcohol to obtain effective components. The traditional propolis alcohol extraction method has unacceptable pungent taste, is easy to generate anaphylactic reaction to alcohol allergic persons when in use, increases the allergen, has great limitation when being used as a raw material of cosmetics and oral health care products, and the propolis alcohol extraction liquid is not easy to dissolve in water, has low absorption utilization rate, and has part of water-soluble active ingredients which are not extracted. Other reported extraction methods include propolis extraction with chloroform, ethyl acetate, dichloromethane, glycerol, oil extraction, supercritical extraction, etc., which are costly and do not completely extract the active ingredients. The reported propolis water extraction also adopts cold water extraction or hot reflux, the extraction efficiency is low, and the damage degree of bioactive components is large.
For a long time, the water extraction technology of the propolis conventionally adopts cold water extraction to obtain the propolis water extract, wherein the content of flavonoid compounds is only 1.914mg/ml, and although the total flavone content of the propolis alcohol extract can reach 51.36mg/ml under corresponding conditions, when the propolis alcohol extract is dissolved in water, 100ml of water can only dissolve 4-6 ml of the propolis alcohol extract. According to the principle of similarity and compatibility, the hydrocarbon compounds such as terpenes and alkenes with weak polarity in the volatile oil are less soluble in ethanol (or ethanol water solution) with strong polarity, so that the content of amino acid compounds such as leucine and the like which can reduce blood sugar in the propolis extracted by the extraction method of the propolis disclosed in the patent is extremely low, and the requirement of actual production cannot be met.
Disclosure of Invention
The present invention is directed to solving one of the technical problems of the prior art or the related art.
Therefore, the technical scheme adopted by the invention is as follows: a method for preparing bee therapy product for reducing blood sugar comprises: the preparation method comprises the following steps: (1) freezing propolis at-40 deg.C to-80 deg.C
Pulverizing into fine powder, and sieving with 80-100 mesh sieve; (2) adding pulverized propolis into the powder
The volume percentage concentration of the times is
Adding surfactant into the ethanol solution, extracting with ultrahigh pressure extraction equipment under 30T600Mpa for heating treatment in the presence of sodium carbonate and sodium bicarbonate
The method comprises the following steps of (1) taking minutes; (3) concentrating the extractive solution, and drying.
The present invention in a preferred example may be further configured to: the operation of the step (1) is as follows: taking propolis raw materials, removing impurities and blowing off dust, and freezing for 2-3 hours at the temperature of-40 ℃ to-50 ℃; adding a grinding medium and a grinding lubricant which account for 3-8 wt% of the propolis raw material, grinding, and sieving with a 80-100 mesh sieve to obtain fine powder for later use.
Further, the grinding medium is one or a mixture of rice hulls or bran, the grinding lubricant is in a particle lubricant structure, and the diameter of the particle lubricant is 20-80 microns.
By adopting the technical scheme, various effective components in the propolis break through the outer barrier structure by utilizing the crushing medium and the grinding lubricating particles, and are released and dissolved in the ethanol extracting solution more efficiently.
The present invention in a preferred example may be further configured to: in the step (2), the surfactant is one or more of lecithin, soybean lecithin, polyethylene glycol 6000 or glyceride, and the dissolving effect is further improved by adding the surfactant.
A bee therapy product for reducing blood sugar comprises the propolis extract prepared by the preparation method, wherein the propolis extract comprises the propolis alcohol extract by mass fraction
The flavone compound of (A),
Acid compound of (2),
Organic acids of (2) and
common and trace elements.
The present invention in a preferred example may be further configured to: the flavonoid compounds comprise flavonoids, flavonols and dihydroflavonoids, and the acid compounds comprise a mixture of multiple leucine, histidine, lysine, arginine, leucine, glutamic acid, glycine and tyrosine.
By adopting the technical scheme, the propolis product is rich in effective substances such as dihydroflavonoids, leucine and the like, the propolis is the flavonoid compound with the most health care value, wherein quercetin and rutin have the most representative effects, the quercetin has the effects of dilating coronary vessels, reducing blood fat and blood pressure and the like, the rutin has the effects of softening capillary vessels, enhancing vascular permeability, reducing cholesterol and the like, and the leucine is beneficial to muscle recovery and can control blood sugar.
The beneficial effects obtained by the invention are as follows:
1. according to the invention, the propolis raw material is fully ground and pulverized in high quality, various active ingredients inside the propolis break through an outer barrier structure by using a grinding medium and grinding lubricating particles, and are more efficiently released and dissolved in an ethanol extracting solution, the content of the extracted active ingredients is high, and the dissolving effect is further improved by adding a surfactant, so that compared with the conventional alcohol extraction method, the same effect can be achieved by only half of the dosage of alcohol extraction, and even better effect can be achieved.
2. In the invention, the propolis extract with the total flavone content as high as 15.65mg/ml can be extracted from the propolis product, while the content of flavonoid compounds in the propolis water extract obtained by conventional ethanol extraction is only 1.914mg/ml, and compared with the traditional extraction mode, the internal acid compounds such as leucine and the like which can effectively reduce blood sugar are purified, so that the blood sugar reducing effect of the bee therapy product is improved.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be noted that the embodiments of the present invention and features of the embodiments may be combined with each other without conflict.
It is to be understood that this description is made only by way of example and not as a limitation on the scope of the invention.
The following describes a method for preparing a hypoglycemic bee therapy product according to some embodiments of the present invention.
The invention provides a preparation method of a bee therapy product for reducing blood sugar, which comprises the following steps: the preparation method comprises the following steps: (1) freezing propolis at-60 deg.C to-80 deg.C for 4 hr, pulverizing into fine powder, and sieving with 100 mesh sieve; (2) adding pulverized propolis into the powder
Adding 80% ethanol solution, adding surfactant, extracting with ultrahigh pressure extraction equipment under 30T600Mpa for 10 min in the presence of sodium carbonate and sodium bicarbonate; (3) concentrating the extractive solution, and drying.
In this example, step (1) operates as follows: taking propolis raw materials, removing impurities and blowing off dust, and freezing for 2-3 hours at the temperature of-40 ℃ to-50 ℃; adding grinding medium and grinding lubricant in an amount of 8 wt% of propolis raw material, pulverizing, and sieving with 80-100 mesh sieve to obtain fine powder.
Further, the grinding medium is one or a mixture of rice hulls or bran, and the grinding lubricant is in a particulate lubricant structure, wherein the diameter of the particulate lubricant is 20-80 microns.
Specifically, various effective components inside the propolis break through the outer barrier structure by utilizing the crushing medium and the grinding lubricating particles, and are released and dissolved in the ethanol extracting solution more efficiently.
In this embodiment, the surfactant in step (2) is one or more of lecithin, soybean lecithin, polyethylene glycol 6000, or glyceride, and the dissolution effect is further improved by adding the surfactant.
A bee therapy product for reducing blood sugar comprises propolis extract prepared by the above preparation method, wherein the propolis extract comprises propolis alcohol extract by mass fraction
The flavone compound of (A),
Acid compound of (2),
Organic acids of (2) and
common and trace elements.
In this embodiment, the flavonoids include flavonoids, flavonols and dihydroflavonoids, and the acids include one or more of leucine, histidine, lysine, arginine, leucine, glutamic acid, glycine and tyrosine.
Specifically, the propolis product is rich in effective substances such as biflavonoid and leucine, the most health-care flavonoid compounds in the propolis are represented by quercetin and rutin, the quercetin has the effects of dilating coronary vessels, reducing blood fat and blood pressure and the like, the rutin has the effects of softening capillary vessels, enhancing vascular permeability, reducing cholesterol and the like, and the leucine is beneficial to muscle recovery and can control blood sugar.
Animal experiments: effect of the extract of the present invention on Tetraoxypyrimidine-induced diabetes model mice
Experimental materials:
1. experimental animals: CR mice, body weight
Each half of male and female, provided by Beijing Weitonglihua laboratory animal technology Limited, and the qualification number: SCXK (Jing) 2007 + 0001.
2. Drugs and reagents: propolis Total Flavonoids extract (obtained from the examples of the present invention) propolis alcohol extract, metformin hydrochloride tablet of Sigma, North Biotech Limited in Total Cholesterol kit of North Biotech Limited in North pharmaceutical chemistry reagent factory of Tianjin Taiyang pharmaceutical Limited in glucose kit of Tianjin pharmaceutical chemistry reagent factory of Tianjin City
3. The instrument comprises the following steps: an AA-800 type full-automatic biochemical analyzer, a Beijing Elma medical electronic instrument Co., Ltd, a Beijing medical centrifuge factory;
healthy and non-injured mice are taken, fasted for 24 hours and normally drunk, allowances are respectively injected with alloxan (40mg/kg) for molding at one time into tail veins, the injection volumes are all O.lml/10go (5% sugar water is drunk to the mice 4 to 32 hours after injection), 7 days after molding, fasted for 16 hours, normally drunk water and blood is taken from eyeground venous plexus of the mice, and the blood sugar is measured by a glucose oxidase method. A blood glucose value higher than 200mg/dl is considered as successful molding.
The drug therapy is to select 60 mice with relatively concentrated blood sugar values in the successfully molded patients, and randomly divide the mice into 6 groups: the model control group, the positive control metformin group, the propolis alcohol extract group and the propolis total flavone extract low and high dose group are respectively provided with purified water and test drugs, and the administration volume is O.2ml/10 g. The administration was continued for 14 days, and blood glucose was measured on days 7 and 14 by the same method as above. Performing statistics by adopting SPSS 11.0;
comparing the experimental result with the model control group by P < O.05# P < O.01; compared with propolis alcohol extract group, # P < 0.05
The results show that: the propolis alcohol extract and the propolis total flavone have the function of reducing blood sugar of diabetes model mice induced by alloxan (P < 0.05, P < 0.01) after being administrated for 7 days and 14 days, the function of the propolis total flavone is slightly superior to that of the propolis alcohol extract (P < 0.05) under the same dosage (320mg/kg), and a certain dose-effect relationship exists between the low dose and the high dose.
In the description herein, the description of the terms "one embodiment," "some embodiments," "specific embodiments," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims.
Claims (7)
1. A preparation method of a bee therapy product for reducing blood sugar is characterized by comprising the following steps: the preparation method comprises the following steps: (1) freezing propolis at-40 deg.C to-80 deg.C
Pulverizing into fine powder, and sieving with 80-100 mesh sieve; (2) adding pulverized propolis into the powder
The volume percentage concentration of the times is
Adding surfactant into the ethanol solution, extracting with ultrahigh pressure extraction equipment under 30T600Mpa for heating treatment in the presence of sodium carbonate and sodium bicarbonate
The method comprises the following steps of (1) taking minutes; (3) concentrating the extractive solution, and drying.
2. The method for preparing bee therapy product for lowering blood sugar according to claim 1, wherein the step (1) is operated as follows: taking propolis raw materials, removing impurities and blowing off dust, and freezing for 2-3 hours at the temperature of-40 ℃ to-50 ℃; adding a grinding medium and a grinding lubricant which account for 3-8 wt% of the propolis raw material, grinding, and sieving with a 80-100 mesh sieve to obtain fine powder for later use.
3. The method of claim 2, wherein the grinding medium is one or a mixture of rice hulls or bran, and the grinding lubricant is in a particulate lubricant structure.
4. The method of claim 3, wherein the particulate lubricant is 20-80 microns in diameter.
5. The method for preparing bee therapy product for lowering blood sugar according to any one of claims 1-4, characterized in that in step (2), the surfactant is one or more of lecithin, soybean lecithin, polyethylene glycol 6000 or glyceride.
6. A bee therapy product for reducing blood sugar, characterized by comprising the propolis extract prepared by the preparation method of any one of claims 1 to 5, wherein the propolis extract comprises the propolis alcohol extract in a mass fraction
The flavone compound of (A),
Acid compound of (2),
Organic acids of (2) and
common and trace elements.
7. The method for preparing bee therapy product for lowering blood sugar as claimed in claim 6, wherein said flavonoid compound comprises flavonoid, flavonols and dihydroflavonoids, and said acid compound comprises one or more of leucine, histidine, lysine, arginine, leucine, glutamic acid, glycine and tyrosine.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210075821.6A CN114306390A (en) | 2022-01-23 | 2022-01-23 | Preparation method of bee therapy product for reducing blood sugar |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210075821.6A CN114306390A (en) | 2022-01-23 | 2022-01-23 | Preparation method of bee therapy product for reducing blood sugar |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1515176A (en) * | 2003-08-25 | 2004-07-28 | 浙江大学 | Water-soluble propolis and its extraction method |
| CN101083915A (en) * | 2004-12-06 | 2007-12-05 | 山本伦大 | Propolis extract with improved water dispersibility, propolis-containing beverage, and method of improving water dispersibility of propolis extract |
| CN103054907A (en) * | 2012-12-05 | 2013-04-24 | 天津隆顺榕发展制药有限公司 | Propolis flavonoid extractive and preparation method thereof |
| CN105560295A (en) * | 2015-04-08 | 2016-05-11 | 王晓丽 | Propolis composition capable of restraining fungi |
| CN106074617A (en) * | 2016-08-24 | 2016-11-09 | 深圳市荣格保健品有限公司 | A kind of propolis extract and extraction process thereof |
| CN110639226A (en) * | 2019-09-24 | 2020-01-03 | 江苏蜂奥生物科技有限公司 | Method for removing impurities in propolis |
-
2022
- 2022-01-23 CN CN202210075821.6A patent/CN114306390A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1515176A (en) * | 2003-08-25 | 2004-07-28 | 浙江大学 | Water-soluble propolis and its extraction method |
| CN101083915A (en) * | 2004-12-06 | 2007-12-05 | 山本伦大 | Propolis extract with improved water dispersibility, propolis-containing beverage, and method of improving water dispersibility of propolis extract |
| CN103054907A (en) * | 2012-12-05 | 2013-04-24 | 天津隆顺榕发展制药有限公司 | Propolis flavonoid extractive and preparation method thereof |
| CN105560295A (en) * | 2015-04-08 | 2016-05-11 | 王晓丽 | Propolis composition capable of restraining fungi |
| CN106074617A (en) * | 2016-08-24 | 2016-11-09 | 深圳市荣格保健品有限公司 | A kind of propolis extract and extraction process thereof |
| CN110639226A (en) * | 2019-09-24 | 2020-01-03 | 江苏蜂奥生物科技有限公司 | Method for removing impurities in propolis |
Non-Patent Citations (1)
| Title |
|---|
| 张中印 等: "中国实用养蜂学", 河南科学技术出版社, pages: 474 - 475 * |
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