WO2006041136A1 - Récipient à échantillon et dispositif d'ajout et d'évacuation de liquide équipé dudit récipient à échantillon - Google Patents

Récipient à échantillon et dispositif d'ajout et d'évacuation de liquide équipé dudit récipient à échantillon Download PDF

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Publication number
WO2006041136A1
WO2006041136A1 PCT/JP2005/018883 JP2005018883W WO2006041136A1 WO 2006041136 A1 WO2006041136 A1 WO 2006041136A1 JP 2005018883 W JP2005018883 W JP 2005018883W WO 2006041136 A1 WO2006041136 A1 WO 2006041136A1
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WO
WIPO (PCT)
Prior art keywords
tip
sample container
dispensing
sample
well
Prior art date
Application number
PCT/JP2005/018883
Other languages
English (en)
Japanese (ja)
Inventor
Tsuyoshi Kawai
Original Assignee
Olympus Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Corporation filed Critical Olympus Corporation
Publication of WO2006041136A1 publication Critical patent/WO2006041136A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/028—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates

Definitions

  • the present invention relates to a sample container and a dispensing apparatus to which the sample container is applied.
  • the bottom surface of the container is required to be flat.
  • the bottom surface is required to be flat, and the deepest part where the solution collects cannot be formed. For this reason, a solution remains in a hollow of a hollow and waste liquid efficiency falls.
  • the tip of the tip must be accurately positioned in the recess.
  • the tip relative position of the tip varies greatly (A in Fig. 14A). Therefore, it is necessary to have a mechanical force or some kind of control mechanism that grasps the tip end position in advance by using a sensor and reflects that information during driving.
  • a sample container more specifically, a container used for biochemical reactions, etc., with the bottom surface of the sample recess being flat.
  • a proposal in which the side of the sample recess is inclined see Patent No. 3488465.
  • some form of control mechanism is required to position the tip of the tip into a container that is not described at all when the dispensing tip is inserted into the recess.
  • the present invention provides a sample container capable of positioning the tip end at a position that reduces the remaining amount of waste liquid without detecting the tip end position, and a dispensing device to which the sample container is applied.
  • An object is to provide a waste liquid apparatus.
  • a sample container includes a well that contains a liquid sample, and the bottom surface of the sample container has a flat surface or a shape similar to that for use in a biochemical reaction or analysis.
  • the method further comprises positioning means for positioning the tip of the tip for dispensing and sucking the sample at the boundary between the side surface and the bottom surface of the well.
  • a dispensing apparatus includes a stage for mounting a sample container provided with the positioning means, and dispensing of a sample into the sample container or aspiration of a used sample. And a dispensing / waste liquid unit for moving the chip horizontally and vertically.
  • FIG. 1 is a diagram showing a schematic configuration of a dispensing / waste liquid apparatus to which a sample container according to an embodiment of the present invention is applied.
  • FIG. 2A is a diagram showing a configuration of a sample container according to an embodiment of the present invention.
  • FIG. 2B is a diagram showing a configuration of a sample container according to an embodiment of the present invention.
  • FIG. 3A is a diagram showing an operation example related to positioning of the tip end of the present invention.
  • FIG. 3B is a diagram showing an operation example related to positioning of the tip end of the present invention.
  • FIG. 4A is a diagram showing a configuration of a sample container according to another embodiment of the present invention.
  • FIG. 4B is a diagram showing a configuration of a sample container according to another embodiment of the present invention.
  • FIG. 5A is a diagram showing a configuration of a sample container according to still another embodiment of the present invention.
  • FIG. 5B is a diagram showing a configuration of a sample container according to still another embodiment of the present invention.
  • FIG. 6 is a view showing a configuration of a sample container according to still another embodiment of the present invention.
  • FIG. 7 is a diagram showing a configuration of a sample container according to still another embodiment of the present invention.
  • FIG. 8 is a diagram showing a configuration of a sample container according to still another embodiment of the present invention.
  • FIG. 9 is a diagram showing a configuration of a sample container according to still another embodiment of the present invention.
  • FIG. 10A is a diagram showing a schematic configuration relating to positioning of a sample container according to the present invention.
  • FIG. 10B is a diagram showing a schematic configuration related to positioning of the sample container according to the present invention.
  • FIG. 11 is a diagram showing a configuration example relating to positioning of a sample container according to the present invention.
  • FIG. 12 is a diagram showing another configuration example relating to positioning of a sample container according to the present invention.
  • FIG. 13 is a diagram showing still another configuration example relating to positioning of the sample container according to the present invention.
  • FIG. 14A is a diagram for explaining a conventional solution inhalation method.
  • FIG. 14B is a diagram for explaining a conventional solution inhalation method.
  • FIG. 1 is a diagram showing a schematic configuration of a dispensing and waste liquid apparatus to which a sample container according to an embodiment of the present invention is applied.
  • the disposable tip 4 is moved to the sample container 6 or the dispensing 'waste liquid container 8 to dispense it.
  • FIGS. 2A and 2B are diagrams showing a configuration of a sample container according to an embodiment of the present invention.
  • FIG. 2A is a perspective view
  • FIG. 2B is a cross-sectional view of one well.
  • the measurement container 14 has four sample wells. For this well, use the system shown in Fig. 1 to dispense the sample and waste liquid.
  • the side surface 15 of the sample well has a conical inclination toward the bottom surface 16.
  • the bottom surface 16 is a field where a hybridization reaction and the like are performed, and is a portion to be directly measured by an analyzer.
  • the cross-hatched part in the figure is the test site for the above reaction and measurement.
  • the disposable tip When dispensing using the system shown in Fig. 1, the disposable tip has a large variation in shape, and there is also an error in attaching the disposable tip to the apparatus. For this reason, variations in the relative positions of the measurement container and the tip of the tip inevitably occur.
  • the side surface 15 of the well is inclined as shown in FIGS. 2A and 2B.
  • the dimension in the direction perpendicular to the chip insertion direction (dimension B and slope width dimension in Fig. 2B) is set to be greater than the variation in the relative position of the measurement container and the tip of the chip.
  • dimension B is 0.5mn! It is more effective when set to ⁇ 8mm.
  • FIGS. 2A and 2B the entire side surface 15 of the well is conical, and the inclined surface is formed.
  • the present invention is not limited to this, and the inclined surface may be formed only in a part. .
  • Figures 4A to 5B are diagrams showing an embodiment in which an inclined portion is provided on a part of the side surface of the well. 4A and 4B, FIG. 4A is a perspective view, and FIG. B is a cross section of one well.
  • 5A and 5B are views showing an embodiment in which a conical inclined portion is provided on a part of the side surface of the well. 5A and 5B, FIG. 5A is a perspective view, and FIG. 5B is a sectional view of one well.
  • the inclined surface may be a curved surface that does not have to be a flat surface having a single inclined surface, or a shape in which two or more flat surfaces are combined (for example, see FIGS. 6 and 7).
  • the present invention is not limited to a cylindrical shape and can be applied to any container as long as it has a dish-shaped container bottom surface. is there.
  • the containers with a dish-shaped bottom shown in Fig. 8 and Fig. 9 are made of a transparent material such as glass or grease, so that they can react with the solution and culture the tissue, and at the same time observe from multiple directions. Make it possible. Even in such a container, it is possible to suck up a tissue tissue solution without leaving the container by positioning a tip in part and positioning the tip as described above.
  • the above method is also used for the purpose of sucking up only the liquid for measurement and various treatments and leaving the tissue on the container. It is effective.
  • the angle of the inclined surface is not particularly mentioned.
  • the tip tip is usually directed toward the tip and the diameter thereof is reduced, at least the tip tip is in contact with the bottom surface of the well.
  • the tip of the tip is flexible or the sample container In any case, the angle is such that the tip of the tip can be easily positioned near the boundary between the bottom surface and the side surface of the well by pressing the tip of the tip.
  • the tip end is highly flexible! In this case, the tip of the tip is pressed against the inclined surface, and the tip of the tip is slid to be positioned at the boundary between the side and the bottom.
  • the configuration of the sample container it is preferable that the configuration of the sample container be as small as possible. An example of the configuration is shown in FIG.
  • the sample container 6 is always urged by an elastic member 17 such as a spring, for example.
  • an elastic member 17 such as a spring, for example.
  • the sample container 6 is slid by the load (pressing) on the inclined surface of the well of the sample container 6 by the tip of the tip, so that damage to the chip 4 and the sample container 6 can be prevented and at the same time Since the inclined surface and tip 4 are in contact with each other, the tip of the tip can be lowered to the target location (the boundary between the inclined surface and the bottom surface). At this time, it is important that the direction in which the sample container 6 is urged is the horizontal direction, and the inclined surface is inclined with respect to the tip 4.
  • FIG. 11 to FIG. 13 show a mechanism for urging the sample container 6.
  • a protrusion 19 for fixing and positioning the sample container is provided on the stage.
  • the sample container is fixed by pressing the sample container with the stopper 20 and the coil panel 18 as an elastic member.
  • Fig. 12 shows the case using the panel panel 21 as the elastic material
  • Fig. 13 shows the case using the elastic material 22 such as silicon rubber.
  • the tip end position is automatically positioned at a predetermined position by the positioning means, the tip end detection mechanism can be omitted, and the apparatus cost can be reduced.
  • the time for detecting the tip is unnecessary, the measurement time can be reduced. In particular, when the number of samples is large, a significant time saving effect can be expected. Power!
  • the positioning with respect to the sample container is automatically performed, the descent speed at the tip of the tip can be increased, leading to a reduction in measurement time.
  • the present invention is not limited to the above-described embodiments, and the gist of the present invention is lost in the implementation stage. Various modifications can be made without departing from the scope. Further, the above embodiments include inventions at various stages, and various inventions can be extracted by appropriately combining a plurality of disclosed constituent elements.
  • the present invention relates to a sample container and a dispensing apparatus to which the sample container is applied.

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

La présente invention a pour objet un récipient à échantillon incluant un puits (5) permettant de stocker un échantillon liquide. La paroi inférieure (16) dudit puits (5) est plane ou quasi-plane en vue d'une utilisation dans une analyse ou une réaction biochimique. Ledit récipient est équipé d'un dispositif de positionnement permettant de positionner l'extrémité pointue de l'embout d'ajout/prélèvement dudit échantillon à la frontière entre la paroi latérale (15) et la paroi inférieure (16).
PCT/JP2005/018883 2004-10-13 2005-10-13 Récipient à échantillon et dispositif d'ajout et d'évacuation de liquide équipé dudit récipient à échantillon WO2006041136A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2004299202 2004-10-13
JP2004-299202 2004-10-13

Publications (1)

Publication Number Publication Date
WO2006041136A1 true WO2006041136A1 (fr) 2006-04-20

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PCT/JP2005/018883 WO2006041136A1 (fr) 2004-10-13 2005-10-13 Récipient à échantillon et dispositif d'ajout et d'évacuation de liquide équipé dudit récipient à échantillon

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WO (1) WO2006041136A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009222555A (ja) * 2008-03-17 2009-10-01 Konica Minolta Medical & Graphic Inc 注入装置、検体前処理装置およびマイクロ検査チップ
JP2013533491A (ja) * 2010-07-27 2013-08-22 インスプヘロ アーゲー コンプライアントマルチウェルプレート
JP5279700B2 (ja) * 2007-07-20 2013-09-04 アークレイ株式会社 検体分析用具
JP2017518763A (ja) * 2014-04-04 2017-07-13 アイティ−アイエス インターナショナル リミテッドＩｔ−Ｉｓ Ｉｎｔｅｒｎａｔｉｏｎａｌ Ｌｔｄ 生化学反応システム

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6126867A (ja) * 1984-07-17 1986-02-06 Konishiroku Photo Ind Co Ltd 生化学分析装置
JPH0244257A (ja) * 1988-08-04 1990-02-14 Yasunobu Tsukioka 分注・吸引装置による薬液・洗滌水の吸引排除方法と分注・吸引装置
JPH0777483A (ja) * 1993-09-08 1995-03-20 Sanyo Electric Co Ltd 分注用プレートの支持装置及び自動分注装置
JPH08297125A (ja) * 1995-04-26 1996-11-12 Suzuki Motor Corp 洗浄液吸引方法及びその装置
JPH11326152A (ja) * 1998-05-08 1999-11-26 Matsushita Electric Ind Co Ltd マイクロタイタープレートおよび分注方法
JP2003302411A (ja) * 2002-04-10 2003-10-24 Matsushita Electric Ind Co Ltd 分注装置
WO2004003504A2 (fr) * 2002-06-28 2004-01-08 Igen International, Inc. Systemes d'essais ameliores et composants associes

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6126867A (ja) * 1984-07-17 1986-02-06 Konishiroku Photo Ind Co Ltd 生化学分析装置
JPH0244257A (ja) * 1988-08-04 1990-02-14 Yasunobu Tsukioka 分注・吸引装置による薬液・洗滌水の吸引排除方法と分注・吸引装置
JPH0777483A (ja) * 1993-09-08 1995-03-20 Sanyo Electric Co Ltd 分注用プレートの支持装置及び自動分注装置
JPH08297125A (ja) * 1995-04-26 1996-11-12 Suzuki Motor Corp 洗浄液吸引方法及びその装置
JPH11326152A (ja) * 1998-05-08 1999-11-26 Matsushita Electric Ind Co Ltd マイクロタイタープレートおよび分注方法
JP2003302411A (ja) * 2002-04-10 2003-10-24 Matsushita Electric Ind Co Ltd 分注装置
WO2004003504A2 (fr) * 2002-06-28 2004-01-08 Igen International, Inc. Systemes d'essais ameliores et composants associes

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5279700B2 (ja) * 2007-07-20 2013-09-04 アークレイ株式会社 検体分析用具
JP2009222555A (ja) * 2008-03-17 2009-10-01 Konica Minolta Medical & Graphic Inc 注入装置、検体前処理装置およびマイクロ検査チップ
JP2013533491A (ja) * 2010-07-27 2013-08-22 インスプヘロ アーゲー コンプライアントマルチウェルプレート
JP2017518763A (ja) * 2014-04-04 2017-07-13 アイティ−アイエス インターナショナル リミテッドＩｔ−Ｉｓ Ｉｎｔｅｒｎａｔｉｏｎａｌ Ｌｔｄ 生化学反応システム
US10919042B2 (en) 2014-04-04 2021-02-16 It-Is International Limited Biochemical reaction system

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