WO2006024203A1 - Dispositif de pipettage a canaux multiples - Google Patents

Dispositif de pipettage a canaux multiples Download PDF

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Publication number
WO2006024203A1
WO2006024203A1 PCT/CN2004/001343 CN2004001343W WO2006024203A1 WO 2006024203 A1 WO2006024203 A1 WO 2006024203A1 CN 2004001343 W CN2004001343 W CN 2004001343W WO 2006024203 A1 WO2006024203 A1 WO 2006024203A1
Authority
WO
WIPO (PCT)
Prior art keywords
frame
push rod
syringe
plate
fixing plate
Prior art date
Application number
PCT/CN2004/001343
Other languages
English (en)
Chinese (zh)
Inventor
Dong Wang
Renyuan Chen
Kun Zou
Jing Cheng
Original Assignee
Capitalbio Corporation
Tsinghua University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Capitalbio Corporation, Tsinghua University filed Critical Capitalbio Corporation
Publication of WO2006024203A1 publication Critical patent/WO2006024203A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/54—Supports specially adapted for pipettes and burettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1065—Multiple transfer devices
    • G01N35/1074—Multiple transfer devices arranged in a two-dimensional array
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type

Definitions

  • the present invention relates to a pipetting device, and more particularly to a multi-channel pipette that can be used with a robot.
  • Pipettes typically use a disposable tip that is attached to the pipette prior to operation.
  • the pipette has a knob to adjust the amount of sample to be taken; after setting the sample volume, press the button at the end of the pipette to discharge the air; put the pipette into the liquid to release the button, the set volume can be set.
  • the liquid is sucked up; then the pipette is placed in the drain position and the liquid is discharged by pressing the button.
  • the pipette is easy to operate, but it has at least three disadvantages: The accuracy is not high; manual operation is required, and human error is easy to introduce; the flux is small, and only a few samples can be transferred at a time.
  • the automatic pipetting device consists of an automatic pipette and a robot, which work together to achieve a pipetting operation.
  • the robot carries an automatic pipette to realize the spatial movement of the pipette between the liquid source position, the target position, and the cleaning position; and the automatic pipette realizes sampling at the source position, suction at the target position under the control of the circuit, Cleaning operation at the cleaning location.
  • Automatic pipettes typically operate simultaneously on multiple channels, such as 4, 8, 12, 24, 96 channels. Automatic pipettes work in many different ways, but most can be divided into two categories: volumetric and temporal. The volumetric method is based on the principle of a syringe pump.
  • the volumetric method is simple in quantification, relatively low in cost, and can be multi-channel simultaneously. jobs.
  • the time rule is easy to achieve a small amount of liquid transfer, but the cost is higher, generally using 4/8/16 channels; at the same time, transferring the same volume of liquid, when the liquid viscosity and density are different, it needs to be determined experimentally.
  • W09926723 discloses an automatic pipette consisting of a pump chamber containing a pump hole of the same number of channels and a plurality of piston push rods, one for each pump hole, and each piston simultaneously moving to achieve Multiple channels simultaneously perform aspiration and nesting operations.
  • the disadvantages of this type of pipette are: To prevent air leakage when the piston moves in the pump hole and to reduce the wear of the piston and the inner wall of the pump, each piston and pump hole must have a good fit and coaxiality. . At the same time, in order to ensure the consistency between the channels, there must be good consistency between the pump holes and between the pistons. This high-precision requirement increases the difficulty of processing and greatly increases the cost. At the same time, once a pump hole is broken, the entire pump cavity has to be replaced, and the maintenance cost is high.
  • the multi-channel pipette comprises a plurality of pipetting devices and a frame for fixing the pipetting device, the pipetting device comprising a needle, a syringe and a push rod, the needle and the syringe
  • the push rod is sleeved in the barrel; the top of the frame is provided with a pressure plate that can move up and down, and the head of the push rod is connected to the bottom surface of the pressure plate.
  • the frame includes a frame and a syringe fixing plate fixedly connected to the frame and having a plurality of through holes; the port of the syringe away from the needle is provided with an outwardly extending positioning ring, and the positioning ring is located at the Between the frame and the syringe fixing plate, the push rod is sleeved in the through hole of the syringe fixing plate.
  • a rubber pad I is usually disposed between the top surface of the frame and the syringe fixing plate, so as to have a certain buffering effect between the end surface of the syringe and the fixing plate of the syringe.
  • the bottom surface of the frame is a guide plate provided with a plurality of through holes, and the needle or the syringe is inserted through the through hole of the guide plate for controlling the direction of the needle.
  • the pressure plate includes a bottom plate and a push rod fixing plate provided with a plurality of through holes, and the bottom plate and the push rod fixing plate press the eccentric shaft to rotate the eccentric shaft to press the two plates to fix the push rod.
  • a rubber mat II is also usually provided between the bottom plate and the push rod fixing plate.
  • the platen is further provided with a platen clamp connected to the pressure plate, through which the pressure plate and the w-axis slider of the manipulator are And a frame clamp connected to the side of the frame, the frame clamp is connected with the W-axis base of the robot, and the frame body and the pressure plate are moved relative to each other by controlling the movement of the slider of the robot, thereby realizing suction and pushing Liquid handling.
  • the number of pipette pipetting devices of the present invention can be adjusted according to actual needs, and can be selected 4 8 12 24 96 and the like.
  • Figure 1 is a schematic view showing the structure of a multi-channel pipette
  • FIG. 2 is a schematic structural view of a pipetting device of a multi-channel pipette
  • 3A is a schematic structural view of a frame of a multi-channel pipette
  • Figure 3B is an overall view of the frame of the multi-channel pipette
  • Figure 4 is a structural view of a pressure plate of a multi-channel pipette
  • Figure 5 is a mounting view between the pressure plate and the platen clamp
  • Figure 6 is a mounting diagram between the frame and the frame fixture
  • Figure 7 is a diagram showing the positional relationship between the frame body and the pressure plate when the multi-channel pipette is aspirated
  • Figure 8 is a diagram showing the movement and connection of various parts of a multi-channel pipette mounted on a robot. The best way to implement the invention
  • the 96-channel pipette of this embodiment is arranged in an array of 12 rows and 8 columns and a pitch of 9.
  • the pipette is composed of a pressure plate 16, a frame 17, and a plate clamp 18 and a frame clamp 19, and the like. It consists of four parts that can be attached to the robot by two clamps.
  • each pipetting device of the pipette is composed of a syringe 1, a needle 15 fixed to the barrel 1, and a pusher 2, and a positioning ring at the end of the syringe away from the needle. 14, to facilitate the fixation of the syringe.
  • the pipetting device can also be obtained by simple processing of a common micro-sampler, for example, grinding the hand-held end of the end of the micro-injector, and then bonding a stainless steel positioning ring 14, which is The outer diameter is greater than the respective mounting apertures on the frame 5, and each syringe and its push rod form a pipetting channel for the pipette.
  • FIG. 3A is a schematic structural view of a frame
  • FIG. 3B is an overall view of the frame.
  • the frame body 17 of the present embodiment is composed of a frame 5, a guide plate 6, a syringe fixing plate 3, a rubber pad 14, and the like, a top surface of the frame 5, a guide plate 6, a syringe fixing plate 3, and
  • the rubber pad 14 has 96 through holes, and the syringes 1 are mounted side by side in the through holes of the upper surface of the frame 5, and the upper end faces thereof are covered with a rubber pad 14 and a syringe fixing plate 3. Cover, fixed.
  • the rubber pad 14 and the syringe fixing plate 3 are also provided with mounting holes for the screws 7, which can be fixed to the frame 5 by screws to form a frame.
  • the pressure plate is composed of a push rod fixing plate 8, a rubber pad 119 having a through hole, a bottom plate 10, and a pressing knob 111 having an eccentric shaft.
  • the push rod 2 passes through the bottom plate 10 and the rubber pad 119, and the head is located between the push rod fixing plate 8 and the bottom plate 10.
  • the eccentric shaft is driven by the tightening knob 111 to generate a pressing force between the two plates to fix the push rod 2 to the push rod 2 Press on the plate.
  • the platen clamp 18 is located on the surface of the platen, and the two are screwed through the mounting holes in the bottom plate of the clamp.
  • the mounting holes on the vertical plate of the platen clamp 18 are used to connect with the W-axis slider of the robot.
  • the frame jig 19 is attached to the side of the frame, the frame is placed in the jig, and the knob 1113 is rotated and tightened, and the frame can be fixed by the pressing force between the knob and the frame.
  • the back of the frame clamp has mounting holes for connection to the W-axis base of the robot.
  • Figure 7 is a diagram showing the positional relationship between the pressure plate and the frame when the pipette is aspirated, showing that the liquid is sucked by the relative movement of the push rod and the syringe.
  • Example 2 Assembly and operation of 96-channel pipette and robot
  • the robot carrying the multi-channel pipette of the present invention for pipetting operation is composed of four moving shaft units.
  • the X and Y axes move in a horizontal plane, the moving directions are 90 degrees with each other; the Z axis moves in a vertical direction; the W axis is connected to the Z axis slider, and the Z axis base is driven by the Z axis slider.
  • the seat is moved up and down relative to each other; the frame and the pressure plate of the pipette are respectively connected with the base and the slider of the W-axis, and the platen moves up and down with respect to the frame by the W-axis slider, thereby realizing the pipetting operation.
  • Figure 8 is a diagram showing the movement and connection relationship of various parts of the multi-channel pipette mounted on the manipulator of the present invention.
  • Example 1 The 96-channel pipette described in Example 1 can be assembled as follows:
  • each push rod moves upward relative to each syringe to draw liquid into the channel; move the robot ⁇ , ⁇ and ⁇ axes, move the pipette to the target plate position, and move the W-axis slider downward, each push rod is opposite
  • the syringe moves downward, the liquid can be discharged from the channel into the target hole; then the pipette is moved to the cleaning position, and the W-axis slider is repeatedly moved up and down to allow the cleaning liquid to continuously enter and exit the channel, thereby cleaning the pipetting.
  • Each channel of the device Repeat the above steps to achieve multiple pipetting operations.
  • the liquid sucked from the source plate can be discharged to a set of positions of one target plate, or can be discharged to a plurality of target plates or a plurality of positions of one target plate.
  • the multi-channel pipettes provided by the invention are independent of each other and can be separately taken out from the pipette. Once a pipetting device fails, it can be easily replaced without replacing other pipetting.
  • the device is convenient to disassemble and assemble, and is easy to maintain, which can greatly reduce the maintenance cost.
  • the pipetting device used is simple to process, and can avoid the use of pump holes with relatively high processing precision, which can greatly reduce the processing difficulty and cost of the pipette; Simple comparison and screening can be performed for each pipetting device, and the pipetting device with large difference in the amount of pipetting is removed, thereby ensuring the consistency of the pipetting amount between the channels.
  • the invention can be conveniently connected to the manipulator during use, and the manipulator can be controlled to control the liquid absorption, drainage and displacement of the pipette, thereby realizing simultaneous and automatic operation of the multi-channel pipette.
  • the multi-channel pipette of the invention has the advantages of convenient assembly, low processing difficulty, low maintenance cost and high pipetting precision, and the single channel is used multiple times when the processed 100 ⁇ ⁇ micro sampler is used as the pipetting device. Transfer of 10 ⁇ l of 50% DMS0 can achieve ⁇ 6% accuracy and 4% cv value.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

La présente invention a trait à un dispositif de pipettage à canaux multiples comportant une pluralité de pipettes et un corps d'encadrement pour la fixation des pipettes. La pipette comporte une tête d'aiguille, une seringue et un poussoir. La tête d'aiguille est en liaison serrée avec la seringue; le poussoir est reçu dans la seringue. Un piston mobile vers le haut et vers le bas est prévu sur la partie supérieure du corps d'encadrement, et la tête du poussoir est reliée à la surface inférieure de celui-ci. Des canaux de pipettage du dispositif de pipettage à canaux multiples sont indépendants les uns des autres, permettant un démontage facile. Le dispositif de pipettage présente des applications variées pour le transfert quantitatif d'échantillons biologiques ou chimiques en de quantités minuscules.
PCT/CN2004/001343 2004-09-03 2004-11-23 Dispositif de pipettage a canaux multiples WO2006024203A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN200420093039.4 2004-09-03
CN2004200930394U CN2721223Y (zh) 2004-09-03 2004-09-03 一种多通道移液器

Publications (1)

Publication Number Publication Date
WO2006024203A1 true WO2006024203A1 (fr) 2006-03-09

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PCT/CN2004/001343 WO2006024203A1 (fr) 2004-09-03 2004-11-23 Dispositif de pipettage a canaux multiples

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CN (1) CN2721223Y (fr)
WO (1) WO2006024203A1 (fr)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8597592B2 (en) 2005-09-09 2013-12-03 Capitalbio Corporation Microvalve controlled precision fluid dispensing apparatus with a self-purging feature and method for use
CN108048318A (zh) * 2017-12-21 2018-05-18 江苏集萃智能制造技术研究所有限公司 一种培养液自动添加设备
CN108579833A (zh) * 2018-07-05 2018-09-28 嘉兴凯实生物科技有限公司 一种排枪臂
CN108956851A (zh) * 2017-05-17 2018-12-07 天津工业大学 一种用于微量滴定进样机构
CN113736627A (zh) * 2021-09-30 2021-12-03 北京卓诚惠生生物科技股份有限公司 一种自替换针头的pcr系统注液装置
CN114570446A (zh) * 2022-03-17 2022-06-03 通用生物(安徽)股份有限公司 一种移液罐装设备

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EP2566618B8 (fr) * 2010-05-03 2014-07-09 Integra Biosciences AG Régulateur de mouvement involontaire pour pipeteur multi-canaux électronique aux controle manuelle
CN102059498A (zh) * 2010-12-31 2011-05-18 陕西国德电气制造有限公司 一种装配工装
CN102880194B (zh) * 2012-09-17 2015-05-20 北京信息科技大学 一种多通道移液板的运动控制系统及控制方法
CN103412075B (zh) * 2013-08-27 2015-06-03 贵州电力试验研究院 变压器油色谱全自动进样装置及操作方法
DE102014013552B3 (de) * 2014-09-12 2015-05-21 Festo Ag & Co. Kg Dosiervorrichtung
CN104708252A (zh) * 2015-03-05 2015-06-17 苏州道众机械制造有限公司 一种机械手配件治具固定块
WO2017119097A1 (fr) * 2016-01-07 2017-07-13 株式会社 エー・アンド・デイ Pipette multicanal
CN106111224B (zh) * 2016-08-02 2018-04-06 苏州金锐启智能装备科技有限公司 96通道自动移液器、及其组装方法和工作方法
CN106955752A (zh) * 2017-04-25 2017-07-18 波顿（上海）生物技术有限公司 一种多孔板取样高度调节器
CN108315212B (zh) * 2018-04-02 2023-11-10 通用生物(安徽)股份有限公司 384孔板引物转移装置
CN110243974B (zh) * 2019-07-12 2022-07-05 广州供电局有限公司 气相色谱检测装置及气相色谱检测系统
CN110975719A (zh) * 2019-12-17 2020-04-10 北京化工大学 一种催化材料的高通量实验配液系统和方法
CN111389476A (zh) * 2020-01-04 2020-07-10 北京罗特思创科技有限公司 多通道柱塞泵及其使用方法
CN114749223B (zh) * 2022-04-19 2023-09-08 英诺维尔智能科技（苏州）有限公司 一种单个通道可独立操作的多通道并行移液单元

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US4158035A (en) * 1978-03-15 1979-06-12 Byrd William J Multiple sample micropipette
CN1280671A (zh) * 1997-11-26 2001-01-17 西伯拉巴公司 多通道移液装置

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
US4158035A (en) * 1978-03-15 1979-06-12 Byrd William J Multiple sample micropipette
CN1280671A (zh) * 1997-11-26 2001-01-17 西伯拉巴公司 多通道移液装置

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8597592B2 (en) 2005-09-09 2013-12-03 Capitalbio Corporation Microvalve controlled precision fluid dispensing apparatus with a self-purging feature and method for use
CN108956851A (zh) * 2017-05-17 2018-12-07 天津工业大学 一种用于微量滴定进样机构
CN108048318A (zh) * 2017-12-21 2018-05-18 江苏集萃智能制造技术研究所有限公司 一种培养液自动添加设备
CN108579833A (zh) * 2018-07-05 2018-09-28 嘉兴凯实生物科技有限公司 一种排枪臂
CN108579833B (zh) * 2018-07-05 2024-05-17 嘉兴凯实生物科技股份有限公司 一种排枪臂
CN113736627A (zh) * 2021-09-30 2021-12-03 北京卓诚惠生生物科技股份有限公司 一种自替换针头的pcr系统注液装置
CN113736627B (zh) * 2021-09-30 2022-04-08 北京卓诚惠生生物科技股份有限公司 一种自替换针头的pcr系统注液装置
CN114570446A (zh) * 2022-03-17 2022-06-03 通用生物(安徽)股份有限公司 一种移液罐装设备

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Publication number Publication date
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