WO2005107783A1

WO2005107783A1 - Food containing extract from bark of pine

Info

Publication number: WO2005107783A1
Application number: PCT/JP2005/006540
Authority: WO
Inventors: Kinya Takagaki
Original assignee: Toyo Shinyaku Co., Ltd.
Priority date: 2004-05-10
Filing date: 2005-03-28
Publication date: 2005-11-17
Also published as: WO2005107778A1

Abstract

A body fat reducing preparation or body fat deposition inhibitor comprising an extract from the bark of pine and a soybean peptide. This body fat deposition inhibitor or body fat reducing preparation exhibits an excellent body fat deposition inhibiting effect or reducing effect.

Description

Description Food technology containing pine bark extract

The present invention relates to a body fat accumulation inhibitor or a body fat reducing agent containing a pine bark extract and soy beptide. More specifically, the present invention relates to a food containing a pine bark extract and a soybean peptide. Background art

Recently, it is said that people are in a period of satiety, and the calorie intake tends to be higher than the calorie consumption.Excessive calories are accumulated as body fat, subcutaneous fat, or fat in the liver. You. In particular, fat accumulation in middle-aged and elderly people causes obesity and the resulting adult diseases, such as high blood pressure, fatty liver, hepatitis, and cirrhosis. I'm familiar. Against this background, it is selected from lipid metabolism improvers containing 3-lactoglobulin as an active ingredient (Japanese Patent Application Laid-Open No. Hei 8-259461), guava leaves, hops, rafuma leaves, gymnema leaves, and sanshin. And lipase inhibitors containing one or more crude drugs or extracts thereof (Japanese Patent Application Laid-Open No. 2001-226274). Disclosure of the invention

However, even if lipid metabolism-improving agents containing the above-mentioned components have been proposed to eliminate obesity, the effect of each component is only an effect on one aspect of the cause of obesity. At present, the effects vary greatly depending on constitution and dietary habits. For this reason, a combination of multiple ingredients that eliminate obesity By doing so, it may be possible to respond to various constitutions and dietary habits. However, there is a problem that the effect differs depending on the components to be combined.

In view of the above problems, the present inventors have intensively studied a combination of components having an effect of suppressing or reducing the accumulation of body fat. As a result, they have found that by preparing a food containing a soybean peptide and a pine bark extract, an excellent effect of suppressing or reducing the accumulation of body fat can be obtained, leading to the present invention.

That is, the present invention relates to a body fat accumulation inhibitor or a body fat reducing agent containing a pine peel extract and a soybean peptide.

The present invention also relates to a food containing a pine bark extract and a soy peptide. According to the present invention, a body fat accumulation inhibitor or a body fat reducer containing a pine bark extract and soy beptide exhibits an excellent body fat accumulation inhibitory or reducing effect by combining them. . In particular, OPC in the pine bark extract is likely to be absorbed into the body, and promotes the excretion of cholesterol and the decomposition of neutral fats in the body, which is considered to have the effect of efficiently metabolizing fats. In addition, a food containing a pine bark extract and a soy peptide is a food excellent in palatability because the soy peptide reduces the characteristic flavor of the pine bark extract. BEST MODE FOR CARRYING OUT THE INVENTION

Hereinafter, embodiments of the present invention will be described. The present invention should not be construed as being limited by the description of the following embodiments, and various changes can be made within the scope of the claims.

(I) Pine bark extract

The pine bark used as the raw material for the pine bark extract is French pine (Pinus Mart ima), larch, Japanese black pine, Japanese pine, Japanese pine, Japanese pine, and butterfly. The bark of a plant belonging to the order Pinaceae, such as Satsumamatsu, Himatsu, Ryukyuenmatsu, Pakshimatsu, Daiomatsu, Shiguchimatsu, and Aneda in Quebec, Canada is preferably used. Above all, bark of French pine (Pinus Martima) is preferably used.

French pine is a marine pine that grows on part of the Atlantic coast of southern France. The skin of this French shore pine contains proanthocyanidins, organic acids, and other bioactive ingredients.

The pine bark extract used in the present invention is obtained by extracting the pine bark with water or an organic solvent. If water is used, hot or hot water is used. When an organic solvent is used, a solvent acceptable for the production of foods or drugs can be used. Examples of such an organic solvent include methanol, ethanol, 1-prono-nore, 2-prono-nore, 1-ptano-nore, 2-butanol, acetone, hexane, hexane, hexane, propylene glycol, and hydrous alcohol. Tanol, hydrous propylene glycol, methylethyl ketone, glycerin, methyl acetate, ethyl acetate, getyl ether, dichloromethane, edible fats and oils, 1,1,1,2-tetrafluoroethane, and 1,1,2-trichloroethane Tens. These water and organic solvent may be used alone or in combination of two or more. In particular, water, ethanol, aqueous ethanol, and aqueous propylene dalicol are preferably used. From the viewpoint of safety when used for foods or pharmaceuticals, water, ethanol, and hydrous ethanol are more preferable, and it is more preferable to extract by heating.

There is no particular limitation on the extraction method from the pine bark, and for example, a heating extraction method, a supercritical fluid extraction method, or the like is used.

Supercritical fluid extraction is a method in which a substance is extracted using a supercritical fluid, which is a fluid that has exceeded the critical point (critical temperature, critical pressure) of gas-liquid. Supercritical fluids include carbon dioxide, ethylene, propane, nitrous oxide (laughing gas), etc. And diacid carbon is preferably used.

The supercritical fluid extraction method includes an extraction step of extracting a target component with a supercritical fluid, and a separation step of separating the target component from the supercritical fluid. In the separation step, any of extraction separation by pressure change, extraction separation by temperature change, and extraction separation using an adsorbent / absorbent may be performed.

Further, a supercritical fluid extraction method using an entrainer addition method may be used. In this method, for example, ethanol, propanol, n-hexane, acetone, toluene, other aliphatic lower alcohols, aliphatic hydrocarbons, aromatics In this method, hydrocarbons and ketones are added in an amount of about 2 to 20 w / v%, and this fluid is made into a supercritical fluid state to extract a target substance. According to this method, the solubility of the target extract, such as proanthocyanidins and catechins, in the extraction solvent can be dramatically increased, or the selectivity of separation can be enhanced. A bark extract can be obtained.

The supercritical fluid extraction method can be operated at relatively low temperatures, so that it can be applied to substances that are degraded and decomposed at high temperatures; the advantage that no extracted fluid remains; and the recycling of solvents is possible, There is an advantage that the desolvation step can be omitted, and the step becomes simple.

Extraction from pine bark may be performed by a liquid carbon dioxide batch method, a liquid carbon dioxide reflux method, a supercritical carbon dioxide reflux method, or the like, in addition to the above method. Extraction from pine bark can be done by combining multiple extraction methods. By combining a plurality of extraction methods, it is possible to obtain pine peel extracts having various compositions.

The pine bark extract obtained by the above extraction is purified by ultrafiltration, column method using an adsorbent carrier (Diaion HP-20, Sephadex-LH20, chitin, etc.) or patch method. Preferred from the aspect of safety, to do. The pine bark extract used in the body fat accumulation inhibitor or body fat reducing agent of the present invention is specifically prepared by the following method, but this is only an example and the present invention is not limited to this method.

1 kg of French pine bark is placed in 3 L of a saturated aqueous solution of sodium chloride and extracted at 100 ° C for 30 minutes to obtain an extract (extraction step). Thereafter, the extract is filtered, and the resulting insolubles are washed with 50 OmL of a saturated solution of chickpea sodium to obtain a washing solution (washing step). The extract and the washing solution are combined to obtain a crude extract of pine bark.

Next, the step of adding 25 OmL of ethyl acetate to the crude extract, separating the mixture, and collecting the ethyl acetate layer is repeated five times. The collected ethyl acetate solutions are combined, directly added to 200 g of anhydrous sodium sulfate, and dehydrated. Then, the ethyl acetate solution is filtered, and the filtrate is concentrated under reduced pressure to 1/5 of the original volume. Pour the concentrated ethyl acetate solution into 2 L of chloroform and stir to collect the resulting precipitate by filtration. After that, the step of dissolving this precipitate in 10 mL of ethyl acetate and adding it again to 1 L of chloroform and precipitating for washing is repeated twice. By this method, for example, about 5 g of a pine bark extract containing 20% by mass or more of proanthocyanidins having a degree of polymerization of 2 to 4 and 5% by mass or more of phytotechins is obtained. Here, the content of the specific component in the extract is a value based on the dry mass of the extract. The same applies hereinafter.

The pine bark extract used in the present invention contains broantocyanidin as one of the main active ingredients. Proanthocyanidins are a group of compounds consisting of condensed polymers having a degree of polymerization of 2 or more and containing flavan-13-diol and / or flavane-13,4-diol as a structural unit. It is a powerful antioxidant produced by plants and is concentrated in plant leaves, bark, fruit bark, and seeds. This proanthocyanidin is a substance that cannot be produced in the human body When a pine bark extract containing this proanthocyanidin is ingested, an excellent lipid metabolism improving effect can be obtained. The pine bark extract contains a polycondensate having a degree of polymerization of 2 or more as proanthocyanidins, and further contains catechins and the like. In particular, proanthocyanidins containing a large amount of polycondensates having a low degree of polymerization are preferably used. As the condensation polymer having a low polymerization degree, a condensation polymer having a polymerization degree of 2 to 30 (2 to 30 mer) is preferable, and a condensation polymer having a polymerization degree of 2 to 10 (2 to 10 mer). ) Is more preferable, and a condensation polymer having a polymerization degree of 2 to 4 (a dimer to a tetramer) is further more preferable. Proanthocyanidins, which are polycondensates (2-4 tetramers) with a degree of polymerization of 2-4, are particularly easy to be absorbed into the body, so they not only promote the excretion of cholesterol, but also promote the degradation of medium fats. Can be obtained more effectively. In the present specification, the above polymer having a degree of polymerization of 2 to 4 is referred to as oligomeric proanthocyanidin (hereinafter, referred to as “OPC”).

It is considered that pentamer or more proanthocyanidins have an effect of promoting cholesterol excretion and inhibition of neutral fat absorption. As the pine bark extract, 1.5% by mass, preferably 20% by mass of a polycondensate having a degree of polymerization of 2 to 4 (2 to 4 mer; that is, OPC). / ₀ or more, more preferably 30 mass. / ₀ in a proportion of, pentamer or more Puroantoshia - Gin 1 0 wt% or more, preferably extracts containing a proportion of more than 1 5% by mass. As described above, the pine bark extract contains OPCs and pentamer or more proanthocyanidins, which can enhance cholesterol excretion, suppress fat absorption, and reduce body fat. Conceivable. Such synergistic action improves lipid metabolism in the body.

The pine bark extract may further contain catechins, and the catechins are preferably contained in a proportion of 5% by mass or more, more preferably 10% by mass or more. Catechins are produced by the above-mentioned extraction method. It can be extracted with anidine (OPC). Catechins are a general term for polyhydroxyflapan-1-ol. As catechins, (+) — catechin (referred to as catechin in a narrow sense), (1) Iepicatechin, (+) — gallocatechin, (1) Iepigallocatechin, epigallocatechin gallate, epika techin gallate, and afzelechin Are known. From the pine bark extract, gallocatechin, afzelechin, and a 3-galloyl derivative of (+)-force tekin or gallocatechin have been isolated in addition to the (+) force tekin described above. Catechins include carcinogenesis, arteriosclerosis prevention, blood pressure increase suppression, platelet aggregation suppression, antiallergic, antiviral, antibacterial, caries prevention, bad breath prevention, normal intestinal flora It is known to have a chemical effect, a scavenging effect of active oxygen-free radicals, and an anti-acid effect. Also, catechins are known to have an anti-diabetic effect of suppressing an increase in blood sugar. Catechins are poorly water-soluble by themselves and have low physiological activity, but in the presence of OPC, they have the property of increasing water solubility and activating at the same time. Therefore, force technics work effectively when taken with OPC.

The catechins are preferably contained in the pine bark extract in an amount of 5% by mass or more. More preferably, the pine bark extract containing 20% by mass or more of OPC and 10% by mass or more of pentamer orchid anthocyanidin preferably contains 5% by mass or more of catechins. For example, when the content of catechins in the pine bark extract is less than 5% by mass, force technics may be added so that the content is 5% by mass or more. A force catechins contained 5 mass ^_0/0 or more, and most be used 2 0 mass% or more and a degree of polymerization of 5 or more pro-Ant Xia two gin 1 0 pine bark extract you containing mass% or more OPC preferable. (II) Soy peptide

The soy peptide used in the present invention is a soy peptide that degrades proteins contained in soy. Refers to a peptide obtained by Specific examples include whole soybeans, defatted soybean flakes, soymilk, isolated soybean protein, alcohol-extracted soybean protein, soybean whey protein, and the like, which are decomposed by protein hydrolase or acid or alkali treatment. Further, the molecular weight of the soybean peptide varies depending on the conditions of the decomposition treatment, but is not particularly limited in the present invention. Normally, it is sufficient if the weight average molecular weight is hydrolyzed in the range of about 100 to 500,000. Such a soy peptide is more soluble in water than soy protein and has a higher absorption rate from the intestinal tract. For the purpose of more efficiently absorbing nutrients or improving solubility in water, a low-molecular-weight soybean peptide obtained by fractionating the above-treated soybean peptide by column chromatography or the like may be used. .

(I I I) Other functional ingredients

Food ingredients that can be added to the food of the present invention include, for example, royal jelly, propolis, vitamins (A, B, C, D, E, K, folic acid, pantothenic acid, biotin, derivatives thereof, etc.), minerals ( Iron, magnesium, calcium, zinc, etc.), selenium, chitin, chitosan, lecithin, polyphenol

(Catechins, anthocyanins, condensed tannins such as proanthocyanidins, hydrolysed tannins such as gallotannine, isoflavones, flavonoids, chlorogenic acids, derivatives thereof), carotenoids (lycopene, astaxanthin, Zeaxanthin, rutin, etc., saponin (ginsesanoid, glycyrrhizic acid, gymnemic acid, etc.), xanthine derivatives (caffeine, etc.), fatty acids, amino acids, proteins (collagen, elastin, etc.), peptides other than soybean peptides (collagen peptides, elastin, etc.) Peptides, sardine peptides, etc.), mucopolysaccharides (hyaluronic acid, chondroitin, dematane, heparan, heparin, ketalan, salts thereof, etc.), and amino sugars (glucosamine, aceti Darukosamin, galactosamine, Asechirugaraku Tosamine, neuraminic acid, acetyl neuraminic acid, hexosamine, their salts, etc., dietary fiber (digestible dextrin, alginic acid, guar gum, pectin, dalcomannan, etc.), oligosaccharides (isomaltooligosaccharides, cyclic oligos) Sugar, etc.) Phospholipids and sphingolipids or their derivatives (phosphatidylcholine, sphingomyelin, ceramide, etc.), sulfur-containing compounds (aliyne, sephaen, taurine, glutathione, methylsulfonylmethane, etc.), sugar alcohols, quinones (koenzym) Q10), lignans (sesamin, etc.), animal and plant extracts containing them, root crops (ゥ kon, ginger, etc.), green leaves of grasses such as young wheat leaf, and cruciferous plants such as kale. Green leaves and the like.

Among the above functional components, components such as vitamins and dietary fiber, components that enhance blood sugar level, blood lipids, and lipid metabolism, components that have a blood pressure rise suppressing effect, antithrombotic effects, anti-inflammatory effects, A component having an effect of preventing a disease disease closely related to a cell adhesion factor such as a tumor action can be suitably contained. Such components include, for example, sulfur-containing organic compounds, ubiquinone, heripoic acid, chlorogenic acids, catechins, sapones, mucopolysaccharides, amino sugars, vitamins B, vitamin K, vitamins Ε, collagen and the like. Further, hesperidin, quercetin, rutin or derivatives thereof having an antioxidant effect can also be suitably used.

In particular, when chlorogenic acids, power technics, α-lipoic acid, ubiquinone, and extracts containing these are contained as body fat reducing agents other than pine husk extract, excellent body fat accumulation-suppressing or reducing effects It is also possible to get

In addition, in order to orally ingest the body fat accumulation inhibitor or body fat reducing agent of the present invention, additives include excipients, bulking agents, binders, thickeners, and milking agents. , Lubricating agents, wetting agents, suspending agents, coloring agents, fragrances, food additives and the like can be added. (IV) Body fat accumulation inhibitor or body fat reducing agent. The body fat accumulation inhibitor or body fat reducing agent of the present invention contains the above pine peel extract and soybean peptide, and optionally contains other soybean peptides. It may contain functional ingredients, nutritional ingredients, additives and the like. Specifically, using these components, processing usually performed by those skilled in the art is performed to prepare various shapes.

For example, the processing method of the body fat accumulation inhibitor or the body fat reducing agent of the present invention is, for example, a method of adding excipients to a pine bark extract and a soy peptide to form a tablet, a pill or the like. Examples thereof include a method of forming a powder and other forms without molding. Other dosage forms include capsules such as hard capsules and soft capsules, powders, granules, liquids, and pastes. It can also be processed into tea bags, candy, etc.

The method of ingesting the body fat accumulation inhibitor or body fat reducing agent of the present invention is not particularly limited. The body fat accumulation inhibitor or body fat reducing agent may be eaten or consumed as it is, or may be dissolved in water, hot water, milk, etc., depending on its shape or preference, or may be leached from its ingredients You may drink.

In addition, the body fat accumulation inhibitor or body fat reducing agent of the present invention generally contains pine bark extract in a body fat accumulation inhibitor or a body fat reducing agent in an amount of 0.0001 to 50% by mass. % Is preferably contained.

In order to obtain the effect, the amount of the body fat accumulation inhibitor or body fat reducing agent of the present invention is set to 0.001 g per day as the lower limit of the pine bark extract. As described above, the weight is preferably 0.02 g or more, more preferably 0.02 g or more, and most preferably 0.04 g or more. In addition, the daily intake is 2.0 g or less, preferably 1.0 g or less, more preferably 0.6 g or less as the upper limit of proanthocyanidins.

Furthermore, soybean used for the body fat accumulation inhibitor or body fat reducing agent of the present invention Although the amount of the peptide is not particularly limited, it is preferably 1 part by mass to 2000 parts by mass, and more preferably 10 parts by mass to 100 parts by mass with respect to 100 parts by mass of the dry weight of the pine bark extract. The parts by mass are more preferable, and the parts by mass of 100 to 100 parts by mass are more preferable.

(V) Food of the present invention

The food of the present invention contains a pine bark extract and a soy peptide. The pine bark extract has a unique flavor, for example, bitterness and astringency derived from components contained in the pine bark extract, as well as bark-specific astringency. By blending soy beptide, the flavor unique to pine bark is masked and has excellent palatability. This makes it easier to incorporate the pine bark extract into foods and makes it possible to apply it to various dosage forms. The amount of the soybean peptide in the pine bark extract for obtaining such a masking effect is not particularly limited, but is preferably 100 parts by mass (dry mass) of the pine bark extract. It is preferable to add the peptide in an amount of 100 parts by mass (dry mass) or more.

Furthermore, by combining the pine bark extract with the soy peptide, even when the amount of the pine bark extract used is small, a superior body fat accumulation effect can be obtained as compared with the case where the pine bark extract is used alone. An effect of suppressing lipid absorption is obtained.

Example

Hereinafter, examples of the present invention will be described. It should be noted that the present invention should not be construed as being limited to the following Examples, and various modifications can be made within the scope of the claims.

(Reference Example 1) Evaluation of fat accumulation inhibitory action and lipid absorption inhibitory action Inhibition of fat accumulation in visceral fat and subcutaneous fat using pine bark extract (OPC content 30 mass./ .; Toyo Shinyaku Co., Ltd.) containing proanthocyanidin at a ratio of 60% by mass as follows: The effect and the effect of suppressing lipid absorption were evaluated.

Fourteen 7-week-old female ICR mice (CLEA Japan, Inc.) were acclimated to a basic diet (MF diet, Oriental Yeast Co., Ltd.) for 1 week. Then, each group was divided into two groups so that the average body weight was almost the same. Next, 40 mass% of tallow and 9 mass of granulated sugar were added to a group of mice. /. , And a test feed containing 5% by mass of the above pine bark extract was allowed to freely ingest (test group). The remaining one group of mice was fed a control diet similar to the test diet but without pine bark extract alone (the control group).

On the 25th day from the start of ingestion, the weight of each rat was measured, and the rate of weight gain was calculated by the following formula (I). Rate of increase (%) = (weight of body weight before ingestion 25-body weight before ingestion) weight before ingestion X 1 00 ■ 1 (I)

The subcutaneous fat was measured by X-ray CT for laboratory animals (trade name: LAT heata; Aro Rikisha). Then, retroperitoneal fat and peripheral fat were excised, and the total mass of these fats (mass of visceral fat) was measured. Further, the blood collected from the fundus of each mouse was used to measure the triglyceride level in the blood using a triglyceride measurement kit (Wako Pure Chemical Industries, Ltd.). Table 1 shows the results.

table 1

*: significant difference at p <0.05

From the results in Table 1, it was found that the test feed containing the pine bark extract reduced the amount of visceral fat and subcutaneous fat, and had an effect of reducing body weight.

(Example 1) Evaluation of fat accumulation inhibitory action and lipid absorption inhibitory action

Inhibition of fat accumulation in visceral fat and subcutaneous fat using pine bark extract (OPC content: 30% by mass; Toyo Shinyaku Co., Ltd.) containing proanthocyanidin at a ratio of 60% by mass as follows: The effect was evaluated.

Fifteen 7-week-old female ICR mice (CLEA Japan) were acclimated to a basic diet (MF diet, Oriental Yeast Co., Ltd.) for 1 week. Then, each group was divided into three groups so that the average body weight was almost the same. Next, one group of mice contains 4% by mass of beef tallow, 9% by mass of granulated sugar, 2% by mass of the above pine bark extract, and 5% by mass of soybean peptide (Fuji Oil Co., Ltd.) The test feed 1 was freely taken (referred to as test group 1). The other group was allowed to freely ingest comparative diet 1 prepared in the same manner as trial diet 1 except that it did not contain the pine bark extract of trial diet 1 (comparative group 1). The remaining groups were allowed free access to comparative feed 2 prepared in the same manner as test feed 1 except that they did not contain the pine bark extract and soy beptide of test feed 1 (comparison group 2). ).

On the 25th day from the start of ingestion, the weight of each rat was measured, the weight gain was calculated in the same manner as in Reference Example 1, and the mass of visceral fat and subcutaneous fat was determined in the same manner as in Reference Example 1. It was measured. Table 2 shows the results. Table 2

The values are the mean soil standard deviation

Based on the results in Table 2, the test feed containing pine bark extract and soybean peptide 1 was the comparative feed containing only soybean peptide and the comparative feed containing no pine bark extract and soybean peptide. It was found to have an excellent effect of suppressing or reducing the accumulation of body fat as compared to 2.

Furthermore, the body fat accumulation inhibitor or the body fat reducing agent of the present invention can be used in combination with soy beptide, even when the pine bark extract content is small, to contain only the pine bark extract of Reference Example 1. It was found that the same effect as the feed could be exerted.

(Example 2) Evaluation of palatability

Pine bark extract, soy peptide and soy protein used in Example 1 (Fuji Oil Co., Ltd.) was used to produce a mixed powder food in the amounts shown in Table 3.

Next, 5 female panelists ingested 1 g of each food as they were, and evaluated the preference on the following criteria, and calculated the average score. The results are shown in Table 3.

0 points: Feel the flavor (bitterness, astringency, and astringency) peculiar to pine bark extract.

1 point: The unique flavor of pine bark extract is felt.

2 points: The flavor peculiar to the pine bark extract is hardly felt. Table 3

From the results in Table 3, it can be seen that the food containing the pine bark extract and soy beptide of the present invention is a food in which the flavor peculiar to pine bark is reduced and has excellent palatability. In addition, it can be seen that by including soybean peptide in the same amount or more as the pine bark extract, it becomes possible to have more excellent palatability. Industrial applicability ADVANTAGE OF THE INVENTION According to this invention, the accumulation | storage or inhibitor of body fat containing a pine bark extract and a soy beptide can obtain the excellent accumulation | storage inhibitory effect and reduction effect of body fat. In particular, OPC in the pine bark extract is easily absorbed into the body, promotes the excretion of cholesterol and the breakdown of neutral fats in the body, and is thought to have the effect of efficiently metabolizing fats. Excellent effects can be obtained by combining the action of the pine bark extract with the soybean peptide.

In addition, the combination of the pine bark extract and the soy peptide reduces the peculiar flavor of the pine bark extract and makes the food excellent in palatability.

Claims

The scope of the claims

1. Body fat accumulation inhibitor or body fat reducer containing pine bark extract and soy beptide

2. Food containing pine bark extract and soy beptide.