WO2004064533A1 - Procede de production enzymatique d'un hydrolysat vegetal - Google Patents

Procede de production enzymatique d'un hydrolysat vegetal Download PDF

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Publication number
WO2004064533A1
WO2004064533A1 PCT/EP2003/012611 EP0312611W WO2004064533A1 WO 2004064533 A1 WO2004064533 A1 WO 2004064533A1 EP 0312611 W EP0312611 W EP 0312611W WO 2004064533 A1 WO2004064533 A1 WO 2004064533A1
Authority
WO
WIPO (PCT)
Prior art keywords
plant
enzymes
hydrolyzate
mixture
bacterial
Prior art date
Application number
PCT/EP2003/012611
Other languages
German (de)
English (en)
Inventor
Günther M. KLETT-LOCH
Per KLÜWER
Lore M. Klett-Loch
Original Assignee
Lore Klett-Loch Gdbr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lore Klett-Loch Gdbr filed Critical Lore Klett-Loch Gdbr
Priority to AU2003283390A priority Critical patent/AU2003283390A1/en
Publication of WO2004064533A1 publication Critical patent/WO2004064533A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/14—Vegetable proteins
    • A23J3/18—Vegetable proteins from wheat
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
    • A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
    • A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
    • A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/185—Vegetable proteins

Definitions

  • the invention relates to a process for the enzymatic preparation of a plant hydrolyzate, in which a mixture of plant and / or bacterial enzymes is allowed to act on comminuted plant material, being used as plant material, the whole plant or its parts, and the resulting plant hydrolyzate from the reaction solution Filtration and subsequent spray drying is obtained.
  • the German patent application 25 39 823 describes a method for the production of teas, ointments, creams from medicinal plants by freeze-drying.
  • the plant matter is first crushed, freeze-dried and then finely pulverized;
  • the plant components thus obtained can be incorporated into teas and ointments.
  • this production method has the disadvantage that the biologically active substances can only be made available to a limited extent for the desired application by lacking or deficient cell disruption.
  • German Patent DE 35 39 492 describes a process for the hydrolysis of comminuted lignocellulose with concentrated hydrochloric acid and subsequent distillative recovery of the hydrochloric acid used and subsequent hydrolysis of the sugar solution formed after dilution with water.
  • German patent application DE 38 43 027 describes a biotechnical process for obtaining oil from oil-containing plant products by enzymatic cleavage in an aqueous medium. By the enzyme system used, the protein goes into solution and the separation of the oil released by the hydrolysis of the protein from the aqueous phase is possible.
  • German patent application DE 41 16 744 describes a process for enzymatic seasoning or seasoning from vegetable raw materials.
  • a protease finely divided in water is added and the substrate hydrolyzed by the protease under mild conditions until a ratio of amino nitrogen to total nitrogen of about 0.5 or more is reached.
  • exopeptidases it is also possible to use exopeptidases in order to achieve a further splitting of the peptides obtained into the amino acids.
  • the various enzymes for forming the spicy flavor can be used in a one-step process in a mixture; however, it is advantageous to add the enzymes in succession in a multistage process.
  • the invention has for its object to develop all ingredients of a plant by a gentle process for the preparation of plant hydrolysates and completely without therapeutic loss in any extraction process yield loss of a therapeutic, cosmetic or otherwise use.
  • This object is achieved by a process for the enzymatic preparation of a plant hydrolyzate in which a mixture of plant and / or bacterial and / or fungal enzymes is applied to a comminuted plant material in an aqueous medium buffered to a pH of 6-8 leaves. As plant material serves the whole plant or its parts.
  • the plant hydrolyzate thus obtained is then filtered from the reaction solution and recovered after subsequent spray drying as a dry powder.
  • the aqueous solution during the hydrolysis has a temperature of less than 60 ° C, preferably between 35 and 55 ° C and the solution is a mixture of preferably cellulolytic and / or pectinolytic vegetable or bacterial enzymes is added.
  • Preferred enzymes are cellulases, pectinases, glucanases, proteases and amylases.
  • a mixture of the abovementioned enzymes derived from animals, plants, fungi and bacteria An example of this is an enzyme mixture consisting of approximately equal proportions of pancreatin, papain, fungal protease and bacterial protease.
  • the various enzymes can be used in a mixture in a one-step process, but it may also be advantageous to use the enzymes in groups in succession in a multi-step process.
  • a further advantageous embodiment is to ground the plant material used to a flour, so as to facilitate the enzymatic digestion.
  • the plant hydrolyzate obtained can be used in many ways in medicinal or cosmetic preparations, but also as an additive to foods. example

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Medical Informatics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • General Chemical & Material Sciences (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Cosmetics (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

L'invention concerne un procédé de production enzymatique d'un hydrolysat végétal, consistant : à laisser agir un mélange d'enzymes végétales et/ou bactériennes sur une substance végétale broyée issue d'une plante entière ou de ses parties, dans un milieu aqueux tamponné à un pH compris entre 6 et 8, et ; à isoler l'hydrolysat végétal obtenu, contenu dans la solution réactionnelle, sous forme de poudre sèche, par filtrage, puis séchage par pulvérisation.
PCT/EP2003/012611 2003-01-22 2003-11-12 Procede de production enzymatique d'un hydrolysat vegetal WO2004064533A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003283390A AU2003283390A1 (en) 2003-01-22 2003-11-12 Method for enzymatically producing a plant hydrolysate

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10302307.0 2003-01-22
DE10302307A DE10302307A1 (de) 2003-01-22 2003-01-22 Verfahren zur enzymatischen Herstellung eines Pflanzenhydrolysats

Publications (1)

Publication Number Publication Date
WO2004064533A1 true WO2004064533A1 (fr) 2004-08-05

Family

ID=32694920

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/012611 WO2004064533A1 (fr) 2003-01-22 2003-11-12 Procede de production enzymatique d'un hydrolysat vegetal

Country Status (3)

Country Link
AU (1) AU2003283390A1 (fr)
DE (1) DE10302307A1 (fr)
WO (1) WO2004064533A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102009019104A1 (de) 2009-04-29 2010-11-04 Bionorica Se Kosmetische oder dermatologische Zusammensetzung enthaltend Hydrolysate aus Pflanzenextrakten

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5645417A (en) * 1979-09-19 1981-04-25 Mochida Pharmaceut Co Ltd Pharmaceutical composition having antiallergic activity
DE3843027A1 (de) * 1988-12-21 1990-06-28 Battelle Institut E V Biotechnisches verfahren zur gewinnung von oel und ggf. fettsaeuren aus oelhaltigen pflanzen
JPH03220389A (ja) * 1990-01-26 1991-09-27 Yoshio Imai 植物の精練方法
DE4116744A1 (de) * 1991-05-23 1992-11-26 Zamek Bernhard Verfahren zur enzymatischen wuerze- oder wuerzmittelherstelluung aus pflanzlichen rohstoffen
WO2002001963A2 (fr) * 2000-06-23 2002-01-10 Societe Des Produits Nestle S.A. Preparation de biohydrolysats

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3605050A1 (de) * 1985-02-23 1986-08-28 Juergen Block Verfahren zum aufschluss von bluetenpollen
US5100679A (en) * 1990-10-03 1992-03-31 Cargill B.V. Method of making a modified proteinaceous product and composition thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5645417A (en) * 1979-09-19 1981-04-25 Mochida Pharmaceut Co Ltd Pharmaceutical composition having antiallergic activity
DE3843027A1 (de) * 1988-12-21 1990-06-28 Battelle Institut E V Biotechnisches verfahren zur gewinnung von oel und ggf. fettsaeuren aus oelhaltigen pflanzen
JPH03220389A (ja) * 1990-01-26 1991-09-27 Yoshio Imai 植物の精練方法
DE4116744A1 (de) * 1991-05-23 1992-11-26 Zamek Bernhard Verfahren zur enzymatischen wuerze- oder wuerzmittelherstelluung aus pflanzlichen rohstoffen
WO2002001963A2 (fr) * 2000-06-23 2002-01-10 Societe Des Produits Nestle S.A. Preparation de biohydrolysats

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ADLER-NISSEN J.: "Enzymic Hydrolysis of Food Proteins", 1986, ELSEVIER APPLIED SCIENCE PUBLISHERS, LONDON AND NEW YORK, XP002271270 *
DATABASE WPI Section Ch Week 198124, Derwent World Patents Index; Class B04, AN 1981-43012D, XP002271271 *
DATABASE WPI Section Ch Week 199145, Derwent World Patents Index; Class D16, AN 1991-329357, XP002271272 *
HANS SEJR OLSEN ET AL: "INDUSTRIAL PRODUCTION AND APPLICATIONS OF A SOLUBLE ENZYMATIC HYDROLYZATE OF SOYA PROTEIN", PROCESS BIOCHEMISTRY, vol. 14, no. 7, July 1979 (1979-07-01), pages 6,8,10 - 11, XP008022471 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102009019104A1 (de) 2009-04-29 2010-11-04 Bionorica Se Kosmetische oder dermatologische Zusammensetzung enthaltend Hydrolysate aus Pflanzenextrakten

Also Published As

Publication number Publication date
DE10302307A1 (de) 2004-08-12
AU2003283390A1 (en) 2004-08-13

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