WO2004059007A3 - Traitement de detection de methylation d'acide nucleique par utilisation d'un echantillon de reference interne - Google Patents

Traitement de detection de methylation d'acide nucleique par utilisation d'un echantillon de reference interne Download PDF

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Publication number
WO2004059007A3
WO2004059007A3 PCT/JP2003/016716 JP0316716W WO2004059007A3 WO 2004059007 A3 WO2004059007 A3 WO 2004059007A3 JP 0316716 W JP0316716 W JP 0316716W WO 2004059007 A3 WO2004059007 A3 WO 2004059007A3
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WO
WIPO (PCT)
Prior art keywords
sample
bisulfite
converted
nucleic acid
dna
Prior art date
Application number
PCT/JP2003/016716
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English (en)
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WO2004059007A2 (fr
Inventor
Ryoichi Asai
Katsutoshi Takahashi
Ali Arjomand
Original Assignee
Nat Inst Of Advanced Ind Scien
Combimatrix K K
Ryoichi Asai
Katsutoshi Takahashi
Ali Arjomand
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nat Inst Of Advanced Ind Scien, Combimatrix K K, Ryoichi Asai, Katsutoshi Takahashi, Ali Arjomand filed Critical Nat Inst Of Advanced Ind Scien
Priority to AU2003290433A priority Critical patent/AU2003290433A1/en
Priority to JP2004562938A priority patent/JP2006524033A/ja
Priority to US10/540,897 priority patent/US20060110741A1/en
Publication of WO2004059007A2 publication Critical patent/WO2004059007A2/fr
Publication of WO2004059007A3 publication Critical patent/WO2004059007A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un procédé détection de la méthylation d'ADN à des sites CpG par utilisation de réseaux d'acides nucléiques et, de préférence, des microréseaux. L'invention concerne spécifiquement, un procédé de production directe d'un échantillon de référence à partir de l'échantillon à tester et de détection simultanée de méthylation sur un grand nombre de sites CpG. Plus spécifiquement, le procédé inventif consiste à diviser un échantillon d'ADN en deux échantillons (un premier échantillon et un second échantillon), à amplifier le premier échantillon d'ADN par un processus d'amplification d'acide nucléique tel que tous les résidus de méthylcytosine soient amplifiés en tant que résidus cytosine non méthylés, traitant le premier échantillon amplifié et le second échantillon (non amplifié) avec du bisulfite afin de convertir les résidus de cytosine non méthylés dans les deux échantillons en résidus de désoxyuraciles, à marquer le second échantillon converti en bisulfite avec un second marqueur fluorescent et le premier échantillon converti en bisulfite avec un premier marqueur fluorescent, les premier et second marqueurs fluorescents présentent des spectres d'excitation et d'émission de fluorescence ne se recouvrant pas, et à hybrider le premier échantillon et le second échantillon sur un dispositif microréseau comprenant une pluralité de sondes de capture d'oligonucléotides conçues afin d'hybrider les sites îlots CpG de l'échantillon d'ADN comme convertis et non convertis par le bisulfite.
PCT/JP2003/016716 2002-12-25 2003-12-25 Traitement de detection de methylation d'acide nucleique par utilisation d'un echantillon de reference interne WO2004059007A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2003290433A AU2003290433A1 (en) 2002-12-25 2003-12-25 Nucleic acid methylation detection process using an internal reference sample
JP2004562938A JP2006524033A (ja) 2002-12-25 2003-12-25 内部基準試料を用いる核酸のメチル化検出方法
US10/540,897 US20060110741A1 (en) 2002-12-25 2003-12-25 Nucleic acid methylation detection process using an internal reference sample

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2002375102 2002-12-25
JP2002-375102 2002-12-25

Publications (2)

Publication Number Publication Date
WO2004059007A2 WO2004059007A2 (fr) 2004-07-15
WO2004059007A3 true WO2004059007A3 (fr) 2008-01-10

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2003/016716 WO2004059007A2 (fr) 2002-12-25 2003-12-25 Traitement de detection de methylation d'acide nucleique par utilisation d'un echantillon de reference interne

Country Status (4)

Country Link
US (1) US20060110741A1 (fr)
JP (1) JP2006524033A (fr)
AU (1) AU2003290433A1 (fr)
WO (1) WO2004059007A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE602005023710D1 (de) * 2004-02-20 2010-11-04 Japan Science & Tech Agency Dna-array zur analyse der dna-methylierung, konstruktionsverfahren dafür sowie verfahren zur analyse der dna-methylierung
US20070037184A1 (en) * 2005-06-16 2007-02-15 Applera Corporation Methods and kits for evaluating dna methylation
US7820385B2 (en) 2006-03-22 2010-10-26 The United States Of America As Represented By The Department Of Health And Human Services, Centers For Disease Control And Prevention Method for retaining methylation pattern in globally amplified DNA
US7901882B2 (en) 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
JP6297983B2 (ja) * 2013-01-16 2018-03-20 ユニバーサル・バイオ・リサーチ株式会社 細胞の識別方法
CN105378107A (zh) * 2013-03-14 2016-03-02 雅培分子公司 多重甲基化-特异性扩增系统和方法
CN114703281B (zh) * 2022-02-07 2024-04-02 博尔诚（北京）科技有限公司 用于睾丸癌筛查的标志物、探针组合物及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002018632A2 (fr) * 2000-09-01 2002-03-07 Epigenomics Ag Procede de determination du degre de methylation de cytosines determinees d'adn genomique dans le contexte sequentiel 5'-cpg-3'
EP1312685A2 (fr) * 2001-11-16 2003-05-21 Nisshinbo Industries, Inc. Matrice d'oligonucleotides pour la détection d'états de methylation

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002018632A2 (fr) * 2000-09-01 2002-03-07 Epigenomics Ag Procede de determination du degre de methylation de cytosines determinees d'adn genomique dans le contexte sequentiel 5'-cpg-3'
EP1312685A2 (fr) * 2001-11-16 2003-05-21 Nisshinbo Industries, Inc. Matrice d'oligonucleotides pour la détection d'états de methylation

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ADORJÁN P ET AL: "Tumour class prediction and discovery by microarray-based DNA methylation analysis", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 30, no. 5, 1 March 2002 (2002-03-01), pages e21, XP002242367, ISSN: 0305-1048 *
BALOG ROBERT P ET AL: "Parallel assessment of CpG methylation by two-color hybridization with oligonucleotide arrays.", ANALYTICAL BIOCHEMISTRY, vol. 309, no. 2, 15 October 2002 (2002-10-15), pages 301 - 310, XP002304763, ISSN: 0003-2697 *
GITAN RAAD S ET AL: "Methylation-specific oligonucleotide microarray: a new potential for high-throughput methylation analysis", GENOME RESEARCH, COLD SPRING HARBOR LABORATORY PRESS, US, vol. 12, no. 1, January 2002 (2002-01-01), pages 158 - 164, XP002208051, ISSN: 1088-9051 *
GITAN RAAD SHEBIB ET AL: "Methylation-specific oligonucleotide microarray: A new potential for high-throughput methylation analysis", PROCEEDINGS OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH ANNUAL MEETING, vol. 42, March 2001 (2001-03-01), & 92ND ANNUAL MEETING OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH; NEW ORLEANS, LA, USA; MARCH 24-28, 2001, pages 564, XP001183741, ISSN: 0197-016X *
SHI H ET AL: "OLIGONUCLEOTIDE-BASED MICROARRAY FOR DNA METHYLATION ANALYSIS: PRINCIPLES AND APPLICATIONS", JOURNAL OF CELLULAR BIOCHEMISTRY, WILEY-LISS INC, US, vol. 88, no. 1, 1 January 2003 (2003-01-01), pages 138 - 143, XP001183807, ISSN: 0730-2312 *

Also Published As

Publication number Publication date
AU2003290433A8 (en) 2004-07-22
AU2003290433A1 (en) 2004-07-22
WO2004059007A2 (fr) 2004-07-15
JP2006524033A (ja) 2006-10-26
US20060110741A1 (en) 2006-05-25

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