WO2004052527A8 - Verfahren und reaktor zur amplifikation von dna - Google Patents

Verfahren und reaktor zur amplifikation von dna

Info

Publication number
WO2004052527A8
WO2004052527A8 PCT/DE2003/004113 DE0304113W WO2004052527A8 WO 2004052527 A8 WO2004052527 A8 WO 2004052527A8 DE 0304113 W DE0304113 W DE 0304113W WO 2004052527 A8 WO2004052527 A8 WO 2004052527A8
Authority
WO
WIPO (PCT)
Prior art keywords
dna
principle
amplification
reactor
production
Prior art date
Application number
PCT/DE2003/004113
Other languages
English (en)
French (fr)
Other versions
WO2004052527A1 (de
Inventor
Peter-Juergen Mueller
Reinhard Guthke
Joerg-Hermann Ozegowski
Original Assignee
Knoell Hans Forschung Ev
Friedrich Schiller Uni Versita
Peter-Juergen Mueller
Reinhard Guthke
Joerg-Hermann Ozegowski
Ahlers Horst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Knoell Hans Forschung Ev, Friedrich Schiller Uni Versita, Peter-Juergen Mueller, Reinhard Guthke, Joerg-Hermann Ozegowski, Ahlers Horst filed Critical Knoell Hans Forschung Ev
Priority to AU2003294652A priority Critical patent/AU2003294652A1/en
Publication of WO2004052527A1 publication Critical patent/WO2004052527A1/de
Publication of WO2004052527A8 publication Critical patent/WO2004052527A8/de

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/45—Magnetic mixers; Mixers with magnetically driven stirrers
    • B01F33/452—Magnetic mixers; Mixers with magnetically driven stirrers using independent floating stirring elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/90—Heating or cooling systems
    • B01F35/92—Heating or cooling systems for heating the outside of the receptacle, e.g. heated jackets or burners
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/90—Heating or cooling systems
    • B01F2035/98—Cooling
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/90—Heating or cooling systems
    • B01F2035/99—Heating
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00788—Three-dimensional assemblies, i.e. the reactor comprising a form other than a stack of plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00851—Additional features
    • B01J2219/00867—Microreactors placed in series, on the same or on different supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00851—Additional features
    • B01J2219/00869—Microreactors placed in parallel, on the same or on different supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00873—Heat exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00889—Mixing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00891—Feeding or evacuation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00925—Irradiation
    • B01J2219/00934—Electromagnetic waves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/0095—Control aspects
    • B01J2219/00952—Sensing operations
    • B01J2219/00954—Measured properties
    • B01J2219/00961—Temperature
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/0095—Control aspects
    • B01J2219/00952—Sensing operations
    • B01J2219/00968—Type of sensors
    • B01J2219/0097—Optical sensors
    • B01J2219/00975—Ultraviolet light
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/0095—Control aspects
    • B01J2219/00986—Microprocessor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/14—Means for pressure control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1861—Means for temperature control using radiation
    • B01L2300/1866—Microwaves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Clinical Laboratory Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft eine nach Prinzipien der Polymerase-Chain-Reaction durchgeführte spezielle DNA-Amplifikation in einem oder einer Vielzahl von bevorzugt miniaturisierten Reaktoren und gegebenfalls deren Anordnung als Array. Die Erfindung ermöglicht die kontinuierliche Produktion grösserer Mengen von DNA z.B. für die Herstellung von DNA-Vakzinen unter den erfindungsgemässen Bedingungen eines weitgehend angenäherten Fliessgleichgewichtes. Sie kann auch zur Beeinflussung der Spezifität bzw. der Fehlerrate bei der DNA-Amplifikation nach Prinzipien einer in-Vitro-Evolution beispielsweise bei der Entwicklung von DNA-Aptamere eingesetzt werden.
PCT/DE2003/004113 2002-12-11 2003-12-10 Verfahren und reaktor zur amplifikation von dna WO2004052527A1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003294652A AU2003294652A1 (en) 2002-12-11 2003-12-10 Method and reactor for the amplification of dna

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10258398.6 2002-12-11
DE10258398A DE10258398A1 (de) 2002-12-11 2002-12-11 Verfahren zur Amplifikation von DNA

Publications (2)

Publication Number Publication Date
WO2004052527A1 WO2004052527A1 (de) 2004-06-24
WO2004052527A8 true WO2004052527A8 (de) 2004-08-19

Family

ID=32477626

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2003/004113 WO2004052527A1 (de) 2002-12-11 2003-12-10 Verfahren und reaktor zur amplifikation von dna

Country Status (3)

Country Link
AU (1) AU2003294652A1 (de)
DE (1) DE10258398A1 (de)
WO (1) WO2004052527A1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1614466A3 (de) * 2004-07-08 2006-05-10 Tecan Trading AG System mit Einrichtung zum Verhindern von Luftblasen in einer Hybridisierkammer und entsprechendes Verfahren
US7615370B2 (en) 2004-07-08 2009-11-10 Tecan Trading Ag System having device for preventing air bubbles in a hybridization chamber and corresponding method
EP2255880B1 (de) * 2005-06-21 2014-05-28 Tecan Trading AG Verfahren zum Verhindern von Luftblasen in Hybridisierkammern
WO2008098094A1 (en) * 2007-02-06 2008-08-14 Network Biosystems, Inc. Devices and methods for the performance of miniaturized in vitro assays
AU2008236612B2 (en) 2007-04-04 2014-09-04 Ande Corporation Methods for rapid multiplexed amplification of target nucleic acids
EP2255010B1 (de) 2008-02-20 2018-05-30 Streck Inc. Thermocycler und probengefäss zur schnellen amplifikation von dna
EP2110175A1 (de) * 2008-03-20 2009-10-21 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren und Vorrichtung zur thermischen Steuerung biologischer und chemischer Reaktionen unter Einsatz von magnetischen Partikeln oder magnetischen Beads und Wechselmagnetfeldern
US20100288059A1 (en) * 2009-05-14 2010-11-18 Streck, Inc. Specimen container, system, and method
EP2443254A2 (de) 2009-06-15 2012-04-25 NetBio, Inc. Verbesserte verfahren für forensische dna-quantifizierung
US9737891B2 (en) 2011-06-01 2017-08-22 Streck, Inc. Rapid thermocycler system for rapid amplification of nucleic acids and related methods
WO2014025398A1 (en) 2012-08-10 2014-02-13 Streck, Inc. Real-time optical system for polymerase chain reaction
EP3495803A1 (de) 2013-06-28 2019-06-12 Streck, Inc. Vorrichtungen für echtzeit-polymerasekettenreaktionen

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD261673B5 (de) * 1987-05-29 1996-07-25 Ahlers Horst Dox Dr-Ing Habil Optoelektronische Sensoranordnung
DE4321254C2 (de) * 1993-06-25 2000-11-30 Horst Ahlers Kraftsensor
DE4435107C1 (de) * 1994-09-30 1996-04-04 Biometra Biomedizinische Analy Miniaturisierter Fluß-Thermocycler
US7015030B1 (en) * 1999-07-28 2006-03-21 Genset S.A. Microfluidic devices and uses thereof in biochemical processes
US6607907B2 (en) * 2000-05-15 2003-08-19 Biomicro Systems, Inc. Air flow regulation in microfluidic circuits for pressure control and gaseous exchange

Also Published As

Publication number Publication date
DE10258398A1 (de) 2004-08-12
AU2003294652A1 (en) 2004-06-30
AU2003294652A8 (en) 2004-06-30
WO2004052527A1 (de) 2004-06-24

Similar Documents

Publication Publication Date Title
WO2001094630A3 (en) Compositions and methods for array-based nucleic acid hybridization
WO2004052527A8 (de) Verfahren und reaktor zur amplifikation von dna
WO2003056030A3 (en) Methods and systems of nucleic acid sequencing
EP1350841A3 (de) Thermostabiler oder thermoactiver DNA-polymerase mit abgeschwächter 3'-5' Exonukleaseaktivität
WO2005010209A3 (de) Verfahren zur reversen transkription und/oder amplifikation von nukleinsäuren
WO2006081583A3 (en) Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
WO2004094635A3 (en) Compositions and methods for using a solid support to purify rna
WO2007076404A3 (en) A method of installing an epoxidation catalyst in a reactor, a process for the preparation of an olefin oxide or a chemical derivable from an olefin oxide, and a reactor suitable for such a process
WO2003020968A3 (de) Verfahren zur analyse von nukleinsäurekettensequenzen und der genexpression
WO2005059096A3 (en) Methods for high fidelity production of long nucleic acid molecules with error control
WO2005059093A3 (de) P ef-tu-expressionseinheiten
WO1994004693A3 (en) Novel plants and processes for obtaining them
WO2005073410A3 (en) Nucleic acid amplification with continuous flow emulsion
DK0675969T3 (da) Fremgangsmåde til mangfoldiggørelse af målrettede nucleinsyresegmenter under anvendelse af grupperet polymerase-kædereaktion
WO2002077165A3 (en) Glycoconjugate synthesis using a pathway-engineered organism
WO2007036526A3 (fr) Reacteur et procede pour le traitement d'une matiere dans un milieu reactionnel fluide
WO2005030984A3 (en) Method for small volume nucleic acid synthesis
EP1676910A4 (de) Genomisch modifizierte zelle
EP2292767A3 (de) Polypeptide mit DNA-Polymeraseaktivität
WO2003083051A3 (en) Multiplex standardized reverse transcriptase-polymerase chain reaction method for assessment of gene expression in small biological samples
EP1264894A4 (de) REAKTIONSSYSTEME ZUR REGENERATION VON ATP UND METHODE ZUR PRüFUNG VON ADENIN NUKLEOTIDEN, METHODE ZUR DETEKTION VON RNA UND METHODE ZUR AMPLIFIKATION VON ATP UNTER VERWENDUNG DESSELBEN
WO2007028833A3 (de) Verfahren zur aktivierung einer nukleinsäure für eine polymerase-reaktion
DE60228089D1 (de) Nukleinsäureamplifikation mit duplexzwischenprodukten
WO2003087386A3 (de) Verfahren zur fermentiven herstellung von schwefelhaltigen feinchemikalien unter verwendung von meth-kodierenden coryneformen bakterien
WO2002086112A3 (en) Nucleic acid molecules encoding starch degrading enzymes

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AE AU BA BR CA CN CU DZ GE HR HU ID IL IN IS JP KR LT MA MK NO NZ PL RU SD SG UA US YU ZA

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR

121 Ep: the epo has been informed by wipo that ep was designated in this application
CFP Corrected version of a pamphlet front page
CR1 Correction of entry in section i

Free format text: IN PCT GAZETTE 26/2004 UNDER (72, 75) DELETE "AHLERS, HORST [DE/DE]; OTTOGERD- MUEHLMANN-STRASSE 31, 07743 JENA (DE)."; UNDER (71, 72) ADD "AHLERS, HORST [DE/DE]; OTTOGERD-MUEHLMANN-STRASSE 31, 07743 JENA (DE)."

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Ref document number: JP