WO2004028684A1 - マイクロ流路利用反応方法 - Google Patents
マイクロ流路利用反応方法 Download PDFInfo
- Publication number
- WO2004028684A1 WO2004028684A1 PCT/JP2003/012172 JP0312172W WO2004028684A1 WO 2004028684 A1 WO2004028684 A1 WO 2004028684A1 JP 0312172 W JP0312172 W JP 0312172W WO 2004028684 A1 WO2004028684 A1 WO 2004028684A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- microchannel
- reaction
- solution
- reaction method
- molecules
- Prior art date
Links
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims abstract description 29
- 239000000376 reactant Substances 0.000 claims abstract description 26
- 239000012530 fluid Substances 0.000 claims abstract description 15
- 230000009471 action Effects 0.000 claims description 8
- 150000001875 compounds Chemical class 0.000 claims description 7
- 238000002493 microarray Methods 0.000 claims 1
- 239000000243 solution Substances 0.000 description 15
- 239000000463 material Substances 0.000 description 10
- 238000004519 manufacturing process Methods 0.000 description 8
- 239000000126 substance Substances 0.000 description 6
- 239000000758 substrate Substances 0.000 description 6
- 230000008859 change Effects 0.000 description 5
- 239000007864 aqueous solution Substances 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 2
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 2
- JVTAAEKCZFNVCJ-REOHCLBHSA-N L-lactic acid Chemical compound C[C@H](O)C(O)=O JVTAAEKCZFNVCJ-REOHCLBHSA-N 0.000 description 2
- CPLXHLVBOLITMK-UHFFFAOYSA-N Magnesium oxide Chemical compound [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 description 2
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 2
- -1 Si / Si 0 2 Substances 0.000 description 2
- MCMNRKCIXSYSNV-UHFFFAOYSA-N Zirconium dioxide Chemical compound O=[Zr]=O MCMNRKCIXSYSNV-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 150000002739 metals Chemical class 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
- PWVRXSQPCQPQHM-UHFFFAOYSA-N 2-(4-aminophenyl)-1h-indol-6-amine Chemical compound C1=CC(N)=CC=C1C1=CC2=CC=C(N)C=C2N1 PWVRXSQPCQPQHM-UHFFFAOYSA-N 0.000 description 1
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 229910052581 Si3N4 Inorganic materials 0.000 description 1
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 229910052586 apatite Inorganic materials 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000002440 industrial waste Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000395 magnesium oxide Substances 0.000 description 1
- 150000001247 metal acetylides Chemical class 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 150000004767 nitrides Chemical class 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- VSIIXMUUUJUKCM-UHFFFAOYSA-D pentacalcium;fluoride;triphosphate Chemical compound [F-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O VSIIXMUUUJUKCM-UHFFFAOYSA-D 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 238000000206 photolithography Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 150000004760 silicates Chemical class 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- HQVNEWCFYHHQES-UHFFFAOYSA-N silicon nitride Chemical compound N12[Si]34N5[Si]62N3[Si]51N64 HQVNEWCFYHHQES-UHFFFAOYSA-N 0.000 description 1
- 229910052814 silicon oxide Inorganic materials 0.000 description 1
- 239000010936 titanium Substances 0.000 description 1
- 229910052719 titanium Inorganic materials 0.000 description 1
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 1
- 229910052721 tungsten Inorganic materials 0.000 description 1
- 239000010937 tungsten Substances 0.000 description 1
- 229910052727 yttrium Inorganic materials 0.000 description 1
- VWQVUPCCIRVNHF-UHFFFAOYSA-N yttrium atom Chemical compound [Y] VWQVUPCCIRVNHF-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00819—Materials of construction
- B01J2219/00822—Metal
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00819—Materials of construction
- B01J2219/00824—Ceramic
- B01J2219/00826—Quartz
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00819—Materials of construction
- B01J2219/00831—Glass
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00819—Materials of construction
- B01J2219/00833—Plastic
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00851—Additional features
- B01J2219/00858—Aspects relating to the size of the reactor
- B01J2219/0086—Dimensions of the flow channels
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00889—Mixing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00891—Feeding or evacuation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/0095—Control aspects
- B01J2219/00952—Sensing operations
- B01J2219/00968—Type of sensors
- B01J2219/0097—Optical sensors
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/2575—Volumetric liquid transfer
Definitions
- the present invention relates to a novel microchannel-based reaction method for efficiently performing various chemical reactions by utilizing an action peculiar to a microchannel on a compound molecule carried on a fluid.
- An object of the present invention is to make it possible to efficiently perform a chemical reaction by using a specific action of a microchannel on a molecule carried by a fluid.
- the present inventors carry out various studies on chemical methods using microchannels, and if the unique action of microchannels is used, chemical reactions that have been difficult to proceed with conventional methods can proceed efficiently. This led to the present invention based on this finding.
- Another object of the present invention is to provide a reaction method using a microchannel, characterized in that a chemical reaction is efficiently carried out by utilizing an effect of changing a molecular orientation or a distribution state of a molecule in a solution.
- the flow when a molecule is carried by a fluid in an extremely fine channel having a width of several 10 ⁇ m or less, the flow forms a laminar flow, or the flow contacts a wall surface. Due to its large specific surface area, it exhibits unique effects caused by flow phenomena, such as a large flow velocity gradient.For example, a long linear molecule condenses and elongates from a rounded state, causing a change in molecular shape. Enables reaction to a specific reaction site hidden inside, orients the molecular arrangement in a certain direction, and reactant molecules naturally aggregate at the center or near the wall of the flow channel depending on conditions. This is a method of performing chemical reactions with high efficiency by taking advantage of this. BRIEF DESCRIPTION OF THE FIGURES
- FIG. 1 is a photomicrograph showing the results of Example 1.
- FIG. 2 is a bar graph showing the results of Example 2.
- FIG. 3 is a confocal laser scanning micrograph showing the results of Example 3.
- the microchannel used in the method of the present invention needs to be provided on an inert material substrate.
- This and inert material, with respect to the solvent and the resulting complex is used the probe molecules and analyte molecules or refers to a material that does not exhibit reactivity, for example, glass, quartz, or silica, Si / Si 0 2, Ceramics such as magnesia, zirconia, alumina, apatite, silicon nitride, and oxides, carbides, nitrides, borides, and silicates of metals such as titanium, aluminum, yttrium, and tungsten can be used. .
- the substrate is not particularly limited as long as it is inert to the reactants to be used, and metals, plastics, and the like can be used.
- the shape of the pace is generally a plate-like body, but if desired, an arc-like body, a spherical body, a granular body, or the like can be used.
- the wavelength of the light used is A material that shows sufficient transparency must be used.
- the microchannel is formed by engraving the width and the depth of 1 to 100 ⁇ , preferably 50 to 40 ⁇ , or approximately the same on these inert material substrates.
- a capillary tube having a size of 1 mm is used.
- the length of the microchannel is not particularly limited and depends on the size of the inert material to be used, but is usually selected in the range of 100 to 100 mm.o
- a commercially available cavity tube may be used as it is, or a mechanical channel using a machine tool such as a microdrill may be used. It can be manufactured by engraving on a substrate by means or by forming a groove by a photolithography technique used for manufacturing a semiconductor integrated circuit and then attaching another substrate.
- the fluid flowing in such an ultrafine flow path flows in a laminar flow without being mixed even with a soluble solvent.
- such an ultra-fine channel has features such as a short diffusion distance of a substance, a large specific contact surface area with a wall surface, and a large flow velocity gradient.
- a fluid in which two types of reactant molecules are separately supported is simultaneously passed through the microchannel.
- the two form a laminar flow, and the chemical reaction proceeds at the interface, condenses in the solution, and elongates the entangled compound molecule having a long-chain or branched structure into a straight state.
- the performance of the chemical reaction is improved by the change in the state of the reactant molecules caused by the above-mentioned specialty of the state of the solution flowing through the microchannel.
- DNA molecules and other long linear molecules are usually in a condensed state, ie, a rounded state, in a solution, but when they are flowing through a microchannel, they are frayed and extend linearly.
- Such a change in the shape of the polymer allows the hidden reaction site to be exposed in the solution, allowing the reaction to proceed at a much higher reaction rate than the conventional method, or the reaction to a specific reaction site to be increased.
- Selectivity can be achieved, and application to sensing devices based on chemical reactions and molecular recognition, which was not possible, can be made possible.
- bio-related compounds such as proteins bind to a specific target with a high degree of selectivity. Then, The importance of used fight (nduced fi tj) is known. Then, when such a biological substance is flowed through the microchannel, due to external factors caused by the specialty of the flow state, the protein changes to a three-dimensional structure that is convenient for the protein to recognize the substrate. .
- the reactant molecules flowing in the micro flow channel are oriented in a certain direction due to the specificity of the flow state. Such an orientation is inevitable unless the reactant molecules are perfectly spherical.
- the reaction site can be aligned in the interface direction by this orientation, and a chemical reaction can be performed with high efficiency.
- the solution is sent to the microchannel by, for example, connecting a syringe and adjusting the solution sending speed and solution sending pressure by mechanical means such as a syringe pump.
- the structure state and orientation state of the reactant molecules can be controlled.
- two or more kinds of reactants may be mixed in advance and a fluid carrying the same may be supplied, or two or more kinds of reactants may be supplied.
- the fluid carrying the body molecules may be supplied simultaneously, or one or more reactant molecules may be immobilized on the wall of the microchannel, and the different reactant molecules may be carried on the fluid and supplied. May be.
- the present invention can be applied to chemical reactions in general, but is particularly useful for pharmaceuticals requiring high purity in small quantities and high varieties, synthesis and analysis of biologically relevant substances, and separation.
- the one that appears white is DNA.
- the condensed DNA expands in a straight chain, It is observed that the particles are oriented in the flow direction.
- the reaction of reducing pyruvate to L-lactic acid by L-lactate dehydrogenase was performed using a batch reactor and a microchannel, and the performance was compared. That, 33 ⁇ ( ⁇ 1 / 1 pyruvate, 2 mu and Pioni - lactic de arsenide Doroge Na one Ze, carried out 4 minutes reaction conditions [rho Eta 7.4 phosphate buffer, 'before the reaction L - maximum absorption of lactate dehydrogenase The yield was determined from the decrease in absorbance at a wavelength of 340 nm.
- Figure 2 compares the yields when the above enzyme reaction was performed in a batch reactor and in a microchannel. Yield when using microphone mouth channel The rate showed a significant yield improvement compared to that of the batch method.
- aqueous solution containing 50 ⁇ 1 / 1 fluorescein and pure water were caused to flow while forming a laminar flow in the microchannel, and the state was observed with a confocal laser single-scanning microscope.
- Figure 3 shows the cross section of the flow channel observed using a confocal laser scanning microscope.
- the aqueous solution containing full-year restain and pure water not containing it flow in a laminar flow without mixing with each other, but there is a part where the two parts are particularly brighter than the surrounding area.
- the specificity of the flow state of the solution flowing through the microchannel causes a change in the state of the structure, orientation, and the like of the reactant molecules, whereby the chemical reaction can be performed with high efficiency. It goes beyond simply improving the reaction rate and yield, and allows the chemical reaction, which hardly progressed in the conventional batch method, to proceed because the reaction site is hidden in a higher-order structure. .
- the method of the present invention not only the synthesis of substances and compounds, but also the analysis and separation of specific substances can be performed.
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/527,987 US20060046308A1 (en) | 2002-09-24 | 2003-09-24 | Method of reaction utilizing microchannel |
AU2003266584A AU2003266584A1 (en) | 2002-09-24 | 2003-09-24 | Method of reaction utilizing microchannel |
DE10393363T DE10393363T5 (de) | 2002-09-24 | 2003-09-24 | Reaktionsverfahren unter Verwendung eines Mikrokanals |
GB0505636A GB2411138A (en) | 2002-09-24 | 2005-03-18 | Method of reaction utilizing microchannel |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2002277954A JP2004113874A (ja) | 2002-09-24 | 2002-09-24 | マイクロ流路利用反応方法 |
JP2002-277954 | 2002-09-24 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2004028684A1 true WO2004028684A1 (ja) | 2004-04-08 |
Family
ID=32040415
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2003/012172 WO2004028684A1 (ja) | 2002-09-24 | 2003-09-24 | マイクロ流路利用反応方法 |
Country Status (6)
Country | Link |
---|---|
US (1) | US20060046308A1 (ja) |
JP (1) | JP2004113874A (ja) |
AU (1) | AU2003266584A1 (ja) |
DE (1) | DE10393363T5 (ja) |
GB (1) | GB2411138A (ja) |
WO (1) | WO2004028684A1 (ja) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2004053417A (ja) * | 2002-07-19 | 2004-02-19 | National Institute Of Advanced Industrial & Technology | マイクロ流路利用分子分析方法 |
US20060275911A1 (en) * | 2005-06-03 | 2006-12-07 | Shih-Yuan Wang | Method and apparatus for moleclular analysis using nanostructure-enhanced Raman spectroscopy |
JP5159247B2 (ja) | 2007-10-26 | 2013-03-06 | キヤノン株式会社 | 検出方法および検出装置 |
JP5765722B2 (ja) | 2009-03-31 | 2015-08-19 | マイクロ化学技研株式会社 | マイクロ流路チップ及びそれを用いた気液相分離方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2000246092A (ja) * | 1999-03-04 | 2000-09-12 | Kawamura Inst Of Chem Res | マイクロケミカルデバイスの製造方法 |
US6200814B1 (en) * | 1998-01-20 | 2001-03-13 | Biacore Ab | Method and device for laminar flow on a sensing surface |
JP2002361002A (ja) * | 2001-06-04 | 2002-12-17 | Minolta Co Ltd | 抽出方法および装置、分離方法および装置 |
JP2003299946A (ja) * | 2002-04-05 | 2003-10-21 | Casio Comput Co Ltd | 化学反応装置及び電源システム |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK1257602T3 (da) * | 2000-02-09 | 2004-03-15 | Clariant Finance Bvi Ltd | Fremgangsmåde til fremstilling af azofarvemidler i mikroreaktorer |
US7169367B2 (en) * | 2002-04-05 | 2007-01-30 | Casio Computer Co., Ltd. | Chemical reaction apparatus and power supply system |
-
2002
- 2002-09-24 JP JP2002277954A patent/JP2004113874A/ja active Pending
-
2003
- 2003-09-24 DE DE10393363T patent/DE10393363T5/de not_active Ceased
- 2003-09-24 US US10/527,987 patent/US20060046308A1/en not_active Abandoned
- 2003-09-24 WO PCT/JP2003/012172 patent/WO2004028684A1/ja active Application Filing
- 2003-09-24 AU AU2003266584A patent/AU2003266584A1/en not_active Abandoned
-
2005
- 2005-03-18 GB GB0505636A patent/GB2411138A/en not_active Withdrawn
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6200814B1 (en) * | 1998-01-20 | 2001-03-13 | Biacore Ab | Method and device for laminar flow on a sensing surface |
JP2000246092A (ja) * | 1999-03-04 | 2000-09-12 | Kawamura Inst Of Chem Res | マイクロケミカルデバイスの製造方法 |
JP2002361002A (ja) * | 2001-06-04 | 2002-12-17 | Minolta Co Ltd | 抽出方法および装置、分離方法および装置 |
JP2003299946A (ja) * | 2002-04-05 | 2003-10-21 | Casio Comput Co Ltd | 化学反応装置及び電源システム |
Also Published As
Publication number | Publication date |
---|---|
US20060046308A1 (en) | 2006-03-02 |
DE10393363T5 (de) | 2005-09-08 |
GB0505636D0 (en) | 2005-04-27 |
JP2004113874A (ja) | 2004-04-15 |
GB2411138A (en) | 2005-08-24 |
AU2003266584A1 (en) | 2004-04-19 |
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