WO2004025272A1 - Pressurized fluid sample injector apparatus and method - Google Patents

Pressurized fluid sample injector apparatus and method Download PDF

Info

Publication number
WO2004025272A1
WO2004025272A1 PCT/US2003/028249 US0328249W WO2004025272A1 WO 2004025272 A1 WO2004025272 A1 WO 2004025272A1 US 0328249 W US0328249 W US 0328249W WO 2004025272 A1 WO2004025272 A1 WO 2004025272A1
Authority
WO
WIPO (PCT)
Prior art keywords
sample
valve
pressure
aspirating needle
loop
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/028249
Other languages
English (en)
French (fr)
Inventor
James E. Usowicz
Theodore D. Ciolkosz
Russell L. Keene
Daniel J. Mccormick
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Waters Investments Ltd
Original Assignee
Waters Investments Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Waters Investments Ltd filed Critical Waters Investments Ltd
Priority to JP2004536388A priority Critical patent/JP4564356B2/ja
Priority to DE10393201.1A priority patent/DE10393201B4/de
Priority to DE10393201T priority patent/DE10393201T5/de
Priority to AU2003268571A priority patent/AU2003268571A1/en
Priority to GB0502759A priority patent/GB2408098B/en
Publication of WO2004025272A1 publication Critical patent/WO2004025272A1/en
Priority to US11/072,906 priority patent/US20050194298A1/en
Anticipated expiration legal-status Critical
Priority to US11/532,234 priority patent/US7951597B2/en
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14—Suction devices, e.g. pumps; Ejector devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14—Suction devices, e.g. pumps; Ejector devices
    • G01N2001/1418—Depression, aspiration
    • G01N2001/1427—Positive displacement, piston, peristaltic
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14—Suction devices, e.g. pumps; Ejector devices
    • G01N2001/1445—Overpressure, pressurisation at sampling point

Definitions

  • the present invention relates to sample handling and injection systems and in particular to apparatus and methods for increasing the speed of the injection cycle.
  • liquid chromatography sample injection a sample is drawn into a needle or capillary and then loaded into a sample loop by pulling the fluid through the needle and any associated tubes into the sample loop.
  • the sample loop is connected to an injection mechanism, such as a pump/detector system, that pushes the sample through a liquid chromatography column where a separation takes place.
  • the sample can be pulled through the system of tubes at a flow rate that is directly related to the vapor pressure of the fluid. If the fluid is drawn through the tubing too quickly, the fluid can vaporize and cause undesirable results in sample integrity as well as sample positioning within the sample loop.
  • sample loading speed is increased significantly by pressurizing the fluid system, thus avoiding vaporization of the fluid. This process allows the sample to be transported through the system faster than in a system that draws the sample into a sample loop without utilizing an elevated pressure. Ultimately, the faster sample loading time reduces the overall cycle time between sample injections.
  • the invention embodies a pressurized sample injector system, which utilizes elevated pressure to aid sample delivery to a sample loop.
  • the sample loop is connected across a multiport valve that allows the sample loop to be alternately connected to the sample loading mechanism and the separation mechanism.
  • the sample loading mechanism consists of an aspirating needle, which has already aspirated a sample from a container, that is sealed to a pressure vessel for the loading operation.
  • the aspirating needle is connected to one side of the sample loop through the multiport valve.
  • a metering syringe is connected to the other side of the sample loop through the multiport valve.
  • a sample is aspirated from a container holding the sample into the sample needle.
  • the needle tip is then connected to the pressure vessel and the pressure assist pump and the fluid path is pressurized.
  • the sample is transported from the aspirating needle to the sample loop by creating a pressure differential across the path from the pressure vessel to the metering syringe.
  • the sample is moved from the sample loop to an analytical column by a gradient pump.
  • the second position of the multiport valve disconnects the metering syringe and the aspirating needle from the sample loop and connects the aspirating needle directly to the metering syringe allowing wash cycle(s) to cleanse the flow path while the injection and separation are proceeding.
  • Fig. 1 depicts part of the apparatus
  • Fig. 2 depicts the steps of sample aspiration
  • Fig. 3 depicts one embodiment of the apparatus during pressurization
  • Fig. 4 depicts the pressurization of a sample
  • Fig. 5 A depicts the apparatus during metering
  • Fig. 5B depicts a partial loop sample in the sample loop after metering
  • Fig. 5C depicts a full loop sample with overfill in the sample loop after metering
  • Fig. 6 depicts one embodiment of the invention.
  • Fig. 1 a simplified method of moving a sample quickly is illustrated.
  • a quantity of sample 10 is held in a container 12.
  • a sample loop 18 is in fluid communication with a first end of a sample loop 18 and the second end of the sample loop 18 is in fluid communication with a metering syringe 20.
  • the fluid path from the needle 14 to the syringe 20 is filled with a solute.
  • a second end of the aspirating needle 14 is placed in the sample 10 through a seal 7 that allows the sample 10 to be held under pressure.
  • the entire fluid path from the sample 10 to the metering syringe 20 is under pressure.
  • a pressure differential is established between the metering syringe 20 and the sample 10 which transports the sample liquid through the needle 14 and into the sample loop more swiftly than in a fluidic system that is not under pressure.
  • the device of Fig. 1 When the needle is moved between successive sealed baths of fluid, the device of Fig. 1 is useful for loading a sequence of fluids into a lengthy loop.
  • the handling of the samples could be simplified, the concentration of the samples can be made consistent and the amount of sample used can be minimized using air gaps between samples and by connecting the sample loop across the ports of a suitably configured multiport valve.
  • the metered sample 11 is typically bracketed by air gaps within the aspirating needle.
  • the aspirating needle starts filled with fluid 9 and, as shown in Fig. 2A, before aspiration the metering syringe (not shown) is drawn back to pull in a volume of air 13 at the tip of the aspirating needle 14.
  • the aspirating needle 14 is placed into the sample 10 and the metering syringe is further pulled back a metered amount which draws a predetermined metered sample portion 11 into the aspirating needle 14.
  • the aspirating needle 14 is then lifted from the sample 10 and a post-sample air gap 15 is drawn into the aspirating needle 14 by the metering syringe.
  • Fig. 3 depicts an embodiment of the invention showing pressurization of the metered sample 11 while in the aspirating needle 14 and the use of a multiport valve 16.
  • the apparatus is shown after aspiration of the metered sample 11.
  • the aspirating needle 14 containing the metered sample 11 is placed in a pressurized fluid 28 held in a pressure vessel 42 adapted for this purpose.
  • the aspirating needle 14 is sealed to the pressure vessel 42 by seal 32.
  • a pressure assist pump 30 is in fluid communication with the pressure vessel 42 to pressurized fluid 28.
  • All of the connections of the present invention substantially seal the sample path from ambient pressure.
  • a relief flow means 36 may be used in conjunction with the pressure assist pump to further regulate the pressure on the pressurized fluid 28.
  • a pressure monitor 38 may be connected to the pressurized fluid line for diagnostic and/or control purposes.
  • Fig. 4 depicts the metered sample 11 , bracketed by air gaps, being pressurized in the aspirating needle 14 before being transported under pressure to the sample loop 18. i one embodiment, the aspirating needle 14 is sealed to the pressure vessel 42 by an O-ring 32. A lip seal or any other means for substantially sealing the aspirating needle 14 to the pressure vessel 42 is appropriate.
  • the multiport valve 16 has two positions: in the first position (illustrated in Fig. 3) ports one and three, two and four, and five and six are fluidically connected; in the second position, ports one and two, three and five and four and six are fluidically connected.
  • the aspirating needle 14 is connected to the first port 1.
  • the sample loop 18 is connected across the multiport valve 16 utilizing ports 3 and 4.
  • the metering syringe 20 is connected to the multiport valve 16 at port 2.
  • a gradient pump (not shown) is connected to the multiport valve 16 at port 5 and an analytical column (not shown) is connected to the multiport valve 16 at port 6.
  • the multiport valve is adapted to operate up to the pressures being provided by the pressure assist pump and/or the gradient pump.
  • the gradient pump and column are maintained in fluid communication by the multiport valve 16 and the aspirating needle 14, sample loop 18 and metering syringe 20 are maintained in fluid communication by the multiport valve 16.
  • the first position is also used to draw the metered sample 11 into the aspirating needle 14 before the transport operation depicted by Fig. 3.
  • the multiport valve 16 maintains the gradient pump, sample loop 18 and analytic column in one fluidic path, while the aspirating needle 14 and metering syringe 20 are maintained in fluid communication in a separate fluidic path.
  • This position allows the gradient pump to push the metered sample 11 from the sample loop 18 onto the analytic column while the rest of the device undergoes a cleaning operation.
  • the metering syringe 20 is for drawing a metered amount of fluid through the sample path.
  • the metering syringe 20 functions by creating a vacuum in the syringe.
  • the vacuum creates a pressure differential between the aspirating needle 14 and the metering syringe 20 that pulls fluid toward the syringe.
  • the device controls how far the leading edge of the metered sample 11 moves along the sample path.
  • the metering syringe 20 can be any pump operating on this principle.
  • Multiport valves having different numbers of ports may be utilized in the device. For instance, a four port valve with two positions could be used to draw the metered sample into a sample loop in one position and isolate the sample loop in the second position. Sample loops so filled could be disconnected and retained for further processing. Control means to position the aspirating needle, control the multiport valve and position the metering syringe are needed to coordinate the components.
  • the pressure assist pump 30 is a wash syringe 34, which may also be used to supply wash fluid for cleansing the sample path.
  • the metering syringe 20 is drawn back creating a pressure differential across the sample path.
  • Fig. 5B illustrates a partial loop placement used to position the metered sample 11 in the sample loop 18 when very small quantities of sample 10 are available.
  • the sample loop 18 extends from port 3 to port 4 of the multiport valve 16 and the metered sample 11 is centered in the loop 18.
  • the metering syringe 20 when the metering syringe 20 is drawn back, it positions the metered sample 11 centered in the sample loop 18. Because the loop is overfilled, the metered sample 11 not only fills the sample loop 18 but also extends past the sample loop ports 3, 4, and usually past the connection port 1 for the aspirating needle 14 and the connection port 2 for the metering syringe 20. This positioning places the air gaps 13, 15 well beyond the sample loop 18.
  • the advantage of the full loop with over fill is that it assures that a known quantity of full strength sample is injected into the analytical column from the sample loop 18 during the loading phase of the cycle.
  • the metered sample 11 is not centered but rather is positioned with the trailing air gap 15 just before sample loop port 3.
  • This embodiment takes account of the fact that sample near the leading air gap 13 may become diluted due to dispersion. Consistent concentration of sample is optimized by minimizing the amount of possibly diluted sample near the leading air gap 13 that is positioned in the sample loop 18, and maximizing the amount of concentrated sample near the trailing air gap 17 positioned in the sample loop 18. Since the metered sample 11 is pressurized within the sample path, the metered sample 11 does not vaporize when it is transported into the sample loop 18.
  • Determination of the pressurization level for optimum performance takes the viscosity of the sample and other fluids, the desired positioning speed and the internal diameters (ID) of the sample needle, the interconnecting tubing, the multiport valve, and the metering syringe into account.
  • ID the internal diameters
  • sample movement speeds 600- 2000 ⁇ L/min were attained.
  • This system exhibited a sample load time up to 10 times greater than could be achieved without pressurization. Pressures beyond 150 psig can be used, but the unpressurized air gap size must be increased significantly creating an undesirable effect on cycle time.
  • Table 1 In the preferred embodiment depicted in Fig. 6, the apparatus is set up to operate in a cycle having two phases: one phase transports the metered sample 11 into the sample loop 18 and the second phase cleans the fluid path while the metered sample 11 is being pushed through the analytical column.
  • the cleaning mechanism shown in the figure is representative only, as cleaning technology is well known in the art.
  • a wash syringe pump is used to pressurize the fluid path during the sample transport phase of the cycle.
  • the wash syringe 34 acting as the pressure assist pump 30, maintains a wash block 42, functioning as the pressure vessel, at the desired pressure.
  • a pressure regulating vent 40 is used to maintain a substantially constant pressure while the aspirating needle 14is in the wash block 42.
  • the wash block 42 is constructed so that excess fluid from the pressure regulating vent and the upper portion of a sealed chamber 29 is directed to a collection area 33 that drains into a waste container 41.
  • the aspirating needle 14 is inserted in the O-ring 32 of the wash block 42 and the volume of fluid between the metering syringe 20 and the pressure assist pump 30 is pressurized to assist sample movement.
  • Naive 44 at the head of the metering syringe 20, is set to provide connection between the multiport valve 16 and the metering syringe 20 during this part of the cycle. Pressure is created in the system by dispensing fluid from the pressure assist pump 30, here the wash syringe 34, and is held constant by the pressure regulating vent 40.
  • the metering syringe 20 meters back a pre-determined volume in order to transport the metered sample 11 from the the aspirating needle 14 into the sample loop 18.
  • the multiport valve 16 is actuated and the sample in the sample loop 18 is connected to the gradient pump and analytical column for injection into the analytical column.
  • the apparatus changes to the cleaning phase.
  • the aspirating needle 14 is withdrawn from the O-ring 32 and held above the collection area 33.
  • Valve 46 changes state to allow the wash syringe to be recharged from a wash reservoir 48.
  • Naive 44 changes state to allow wash fluid to be supplied from wash reservoir 48 to the line connected to multiport valve port 2, through the multiport valve 16 to the aspirating needle 14 where it is flushed into the waste 41.
  • wash fluid typically supplied from wash reservoir 48 to the line connected to multiport valve port 2, through the multiport valve 16 to the aspirating needle 14 where it is flushed into the waste 41.
  • the invention allows a sample to be transported into a sample loop in significantly less time than it takes at atmospheric pressure, where transport speed is constrained by the vapor pressure of the fluids transported. Sample positioning accuracy is also improved over other chromatography systems as well.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Hydrology & Water Resources (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
PCT/US2003/028249 2002-09-11 2003-09-10 Pressurized fluid sample injector apparatus and method Ceased WO2004025272A1 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
JP2004536388A JP4564356B2 (ja) 2002-09-11 2003-09-10 加圧流体試料インジェクタおよび流体試料の射出方法
DE10393201.1A DE10393201B4 (de) 2002-09-11 2003-09-10 Hochdruckfluidprobeninjektionsvorrichtung und Verfahren
DE10393201T DE10393201T5 (de) 2002-09-11 2003-09-10 Hochdruckfluidprobeninjektionsvorrichtung und Verfahren
AU2003268571A AU2003268571A1 (en) 2002-09-11 2003-09-10 Pressurized fluid sample injector apparatus and method
GB0502759A GB2408098B (en) 2002-09-11 2003-09-10 Pressurized fluid sample injector and method of injecting fluid samples
US11/072,906 US20050194298A1 (en) 2002-09-11 2005-03-04 Pressurized fluid sample injector and method of injecting fluid samples
US11/532,234 US7951597B2 (en) 2002-09-11 2006-09-15 Pressurized fluid sample injector and method of injecting fluid samples

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US40983602P 2002-09-11 2002-09-11
US60/409,836 2002-09-11

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/072,906 Continuation US20050194298A1 (en) 2002-09-11 2005-03-04 Pressurized fluid sample injector and method of injecting fluid samples

Publications (1)

Publication Number Publication Date
WO2004025272A1 true WO2004025272A1 (en) 2004-03-25

Family

ID=31994012

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/028249 Ceased WO2004025272A1 (en) 2002-09-11 2003-09-10 Pressurized fluid sample injector apparatus and method

Country Status (6)

Country Link
US (1) US20050194298A1 (https=)
JP (1) JP4564356B2 (https=)
AU (1) AU2003268571A1 (https=)
DE (2) DE10393201T5 (https=)
GB (1) GB2408098B (https=)
WO (1) WO2004025272A1 (https=)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007109529A2 (en) 2006-03-17 2007-09-27 Waters Investments Limited Device and methods for reducing pressure and flow perturbations in a chromatographic system
CN103675174A (zh) * 2013-11-15 2014-03-26 国网山东省电力公司青岛供电公司 一种色谱分析仪
WO2014085003A3 (en) * 2012-11-30 2014-07-31 Agilent Technologies, Inc. Mixer bypass sample injection for liquid chromatography
CN105675776A (zh) * 2016-04-07 2016-06-15 中科合成油技术有限公司 一种在线色谱稳定进样装置及方法
US9435773B2 (en) 2009-06-03 2016-09-06 Agilent Technologies, Inc. Sample injector with metering device balancing pressure differences in an intermediate valve state
US10386342B2 (en) 2008-01-25 2019-08-20 Dionex Softron Gmbh Sample injector for liquid chromatography, particularly for high performance liquid chromatography
US10473631B2 (en) 2015-06-25 2019-11-12 Dionex Softron Gmbh Sampler for liquid chromatography
EP3617701A1 (en) * 2018-08-27 2020-03-04 F. Hoffmann-La Roche AG Sample injector with sample loop and buffer loop

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8241013B2 (en) * 2005-10-27 2012-08-14 Waters Technologies Corporation Serial capillary pump
JP4955342B2 (ja) * 2006-08-28 2012-06-20 株式会社日立ハイテクノロジーズ 液体クロマトグラフ装置及び試料導入装置
US8794052B2 (en) * 2009-04-16 2014-08-05 Shimadzu Corporation Liquid chromatograph
JP5111476B2 (ja) * 2009-10-26 2013-01-09 株式会社日立ハイテクノロジーズ 液体試料分析装置及び液体試料導入装置
JP5270771B2 (ja) * 2012-02-03 2013-08-21 株式会社日立ハイテクノロジーズ 液体クロマトグラフ装置及び試料導入装置
AU2016228931B2 (en) * 2015-03-06 2020-07-09 Alcoa Of Australia Limited Online sampling device
WO2018134662A1 (en) * 2017-01-17 2018-07-26 Waters Technologies Corporation Systems, methods, and devices for providing pressurized solvent flow

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4756201A (en) * 1985-09-03 1988-07-12 Technicon Instruments Corporation Apparatus and method for combined closed and open tube sampling
US4836038A (en) * 1988-03-18 1989-06-06 Aim Instruments Ltd. Automated sampler-injector apparatus and method for sampling a quantity of sample and testing portions of said quantity
US5297431A (en) * 1992-06-01 1994-03-29 Thermo Separation Products (California) Inc. Automated sample dilution
US5814742A (en) * 1996-10-11 1998-09-29 L C Packings, Nederland B.V. Fully automated micro-autosampler for micro, capillary and nano high performance liquid chromatography

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1346301A (en) * 1965-06-04 1974-02-06 Adler Auto Precision Ltd Methods for mixing and or dispensing liquids and apparatus therefor
JPS4843836B1 (https=) * 1970-01-30 1973-12-20
US3988921A (en) * 1974-02-08 1976-11-02 Lightner Gene E Method and apparatus for spotting chromatographic elements
US3918913A (en) * 1974-12-02 1975-11-11 Lilly Co Eli Sampler-injector for liquid chromatography
JPS5342693U (https=) * 1976-09-17 1978-04-12
US4116046A (en) * 1977-08-05 1978-09-26 Hoffmann-La Roche Inc. Liquid chromatography system
US4242909A (en) 1979-04-19 1981-01-06 Rheodyne Incorporated Sample injector
DE3129833A1 (de) * 1981-07-29 1983-02-17 Bodenseewerk Perkin-Elmer & Co GmbH, 7770 Überlingen Probengeber fuer gaschromatographen
US4457846A (en) * 1982-07-08 1984-07-03 Milton Roy Company Liquid chromatography methods and devices
JPS6176955A (ja) 1984-09-21 1986-04-19 Hitachi Ltd 液体試料注入装置
JPS61114143A (ja) * 1984-11-09 1986-05-31 Hitachi Ltd 試料導入装置の導入方法
IT1177184B (it) * 1984-11-14 1987-08-26 Erba Strumentazione Procedimento ed apparecchiatura per analisi chimiche mediante cromatografia liquida
US4674323A (en) * 1985-07-31 1987-06-23 The Dow Chemical Company Self-diagnostic gel permeation/size exclusion chromatograph
JPS6232365A (ja) * 1985-08-06 1987-02-12 Japan Spectroscopic Co 分析装置における液体試料の吸引方法
JPS6250659A (ja) * 1985-08-29 1987-03-05 Shimadzu Corp 液体クロマトグラフへの試料導入方法
JPS62182665A (ja) * 1985-10-15 1987-08-11 Toyo Soda Mfg Co Ltd 微量液の分注方法およびこれに用いる装置
US4920060A (en) * 1986-10-14 1990-04-24 Hercules Incorporated Device and process for mixing a sample and a diluent
DE69124556T2 (de) * 1990-09-11 1997-09-11 Prince Technologies B V Verfahren und Vorrichtung zur Einführung mindestens eines Flüssigkeitsvolumens in eine Röhre, insbesondere für kapillare Elektrophoresesysteme und Verfahren und Vorrichtung zur Trennung und/oder Analyse eines fluiden Materials
EP0607486B1 (en) * 1993-01-21 1997-03-12 Hewlett-Packard GmbH Method and apparatus for injecting liquid into a capillary tube
JPH07311187A (ja) * 1994-05-13 1995-11-28 Shiseido Co Ltd 液体クロマトグラフィ装置における試料注入方法及び注入装置
US5531959A (en) * 1994-01-31 1996-07-02 Hamilton Company Automated liquid handling and computer controlled system and method for solid phase chromatographic extractions
US5667657A (en) * 1995-09-28 1997-09-16 Dionex Corporation Pressure injection apparatus and method for injecting a sample into an electrophoresis capillary
US6299767B1 (en) * 1999-10-29 2001-10-09 Waters Investments Limited High pressure capillary liquid chromatography solvent delivery system
US6749749B2 (en) * 2002-06-26 2004-06-15 Isco, Inc. Separation system, components of a separation system and methods of making and using them

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4756201A (en) * 1985-09-03 1988-07-12 Technicon Instruments Corporation Apparatus and method for combined closed and open tube sampling
US4836038A (en) * 1988-03-18 1989-06-06 Aim Instruments Ltd. Automated sampler-injector apparatus and method for sampling a quantity of sample and testing portions of said quantity
US5297431A (en) * 1992-06-01 1994-03-29 Thermo Separation Products (California) Inc. Automated sample dilution
US5814742A (en) * 1996-10-11 1998-09-29 L C Packings, Nederland B.V. Fully automated micro-autosampler for micro, capillary and nano high performance liquid chromatography

Cited By (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1996930A4 (en) * 2006-03-17 2011-01-19 Waters Technologies Corp DEVICE AND METHOD FOR REDUCING THE PRESSURE AND SUPPRESSING RIVER FAILURES IN A CHROMATOGRAPHIC SYSTEM
US8312762B2 (en) 2006-03-17 2012-11-20 Waters Technologies Corporation Device and methods for reducing pressure and flow perturbations in a chromatographic system
WO2007109529A2 (en) 2006-03-17 2007-09-27 Waters Investments Limited Device and methods for reducing pressure and flow perturbations in a chromatographic system
EP1996930B1 (en) 2006-03-17 2018-01-10 Waters Technologies Corporation Device and methods for reducing pressure and flow perturbations in a chromatographic system
US10386342B2 (en) 2008-01-25 2019-08-20 Dionex Softron Gmbh Sample injector for liquid chromatography, particularly for high performance liquid chromatography
US12372500B2 (en) 2008-01-25 2025-07-29 Dionex Softron Gmbh Sample injector for liquid chromatography, particularly for high performance liquid chromatography
US11802854B2 (en) 2008-01-25 2023-10-31 Dionex Softron Gmbh Sample injector for liquid chromatography, particularly for high performance liquid chromatography
US11156589B2 (en) 2008-01-25 2021-10-26 Dionex Softron Gmbh Sample injector for liquid chromatography, particularly for high performance liquid chromatography
US11519884B2 (en) 2009-06-03 2022-12-06 Agilent Technologies, Inc. Sample injector with metering device balancing pressure differences in an intermediate valve state
US9435773B2 (en) 2009-06-03 2016-09-06 Agilent Technologies, Inc. Sample injector with metering device balancing pressure differences in an intermediate valve state
US10209229B2 (en) 2012-11-30 2019-02-19 Agilent Technologies, Inc. Mixer bypass sample injection for liquid chromatography
US9945820B2 (en) 2012-11-30 2018-04-17 Agilent Technologies, Inc. Mixer bypass sample injection for liquid chromatography
WO2014085003A3 (en) * 2012-11-30 2014-07-31 Agilent Technologies, Inc. Mixer bypass sample injection for liquid chromatography
CN103675174A (zh) * 2013-11-15 2014-03-26 国网山东省电力公司青岛供电公司 一种色谱分析仪
US11307178B2 (en) 2015-06-25 2022-04-19 Dionex Softron Gmbh Sampler for liquid chromatography
US10473631B2 (en) 2015-06-25 2019-11-12 Dionex Softron Gmbh Sampler for liquid chromatography
US11867669B2 (en) 2015-06-25 2024-01-09 Dionex Softron Gmbh Sampler for liquid chromatography
CN105675776A (zh) * 2016-04-07 2016-06-15 中科合成油技术有限公司 一种在线色谱稳定进样装置及方法
CN110865132A (zh) * 2018-08-27 2020-03-06 豪夫迈·罗氏有限公司 样品注射器
US11408865B2 (en) 2018-08-27 2022-08-09 Roche Diagnostics Operations, Inc. Sample injector
EP3617701A1 (en) * 2018-08-27 2020-03-04 F. Hoffmann-La Roche AG Sample injector with sample loop and buffer loop

Also Published As

Publication number Publication date
JP4564356B2 (ja) 2010-10-20
GB2408098B (en) 2006-03-22
GB2408098A (en) 2005-05-18
US20050194298A1 (en) 2005-09-08
AU2003268571A1 (en) 2004-04-30
DE10393201B4 (de) 2023-11-16
JP2005538378A (ja) 2005-12-15
GB0502759D0 (en) 2005-03-16
DE10393201T5 (de) 2005-09-01

Similar Documents

Publication Publication Date Title
WO2004025272A1 (en) Pressurized fluid sample injector apparatus and method
CN102597764B (zh) 液体试样分析装置以及液体试样导入装置
CN102869994B (zh) 吐出装置和液体分注装置以及液体分注方法
US4836038A (en) Automated sampler-injector apparatus and method for sampling a quantity of sample and testing portions of said quantity
US9664653B2 (en) Method for feeding a sample into an analysis branch of a liquid chromatography system
US4242909A (en) Sample injector
US7951597B2 (en) Pressurized fluid sample injector and method of injecting fluid samples
JP5471846B2 (ja) 液体試料導入装置及び液体試料導入方法
EP2668988B1 (en) Channel bubble reduction device, channel bubble reduction program, channel bubble reduction method, liquid provision device and chromatography device
JP6208440B2 (ja) 気泡低減装置、クロマトグラフィ装置、気泡低減方法、及び気泡低減プログラム
JP2005538378A5 (https=)
US5108708A (en) Aliquot collection adapter for HPLC automatic injector enabling simultaneous sample analysis and sample collection
CN107430103B (zh) 自动取样器
JPH06148157A (ja) 試料液供給装置
CN112881393B (zh) 样本检测过程中的吸液方法
JP2012247440A (ja) 液体試料分析装置及び液体試料導入装置
US12306144B2 (en) Mobile phase delivery system and mobile phase delivery method
CN115200655A (zh) 计量装置和计量液体介质的方法
CN116242765B (zh) 一种流式细胞仪的流体系统
JPH09274025A (ja) ガスクロマトグラフ用試料注入装置
JPH0369576B2 (https=)
JPH09127077A (ja) 試料注入装置

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
ENP Entry into the national phase

Ref document number: 0502759

Country of ref document: GB

Kind code of ref document: A

Free format text: PCT FILING DATE = 20030910

WWE Wipo information: entry into national phase

Ref document number: 0502759.4

Country of ref document: GB

WWE Wipo information: entry into national phase

Ref document number: 11072906

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: 2004536388

Country of ref document: JP

122 Ep: pct application non-entry in european phase
WWW Wipo information: withdrawn in national office

Ref document number: 0502759.4

Country of ref document: GB