WO2003091431A8 - Methylotrophe produisant une chaine de sucre de type mammifere - Google Patents

Methylotrophe produisant une chaine de sucre de type mammifere

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Publication number
WO2003091431A8
WO2003091431A8 PCT/JP2003/005464 JP0305464W WO03091431A8 WO 2003091431 A8 WO2003091431 A8 WO 2003091431A8 JP 0305464 W JP0305464 W JP 0305464W WO 03091431 A8 WO03091431 A8 WO 03091431A8
Authority
WO
WIPO (PCT)
Prior art keywords
sugar chain
type sugar
mammalian type
methylotroph
mammalian
Prior art date
Application number
PCT/JP2003/005464
Other languages
English (en)
Japanese (ja)
Other versions
WO2003091431A1 (fr
Inventor
Kazuo Kobayashi
Yoshinori Kitagawa
Toshihiro Komeda
Nagako Kawashima
Yoshifumi Jigami
Yasunori Chiba
Original Assignee
Kirin Brewery
Aist
Kazuo Kobayashi
Yoshinori Kitagawa
Toshihiro Komeda
Nagako Kawashima
Yoshifumi Jigami
Yasunori Chiba
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kirin Brewery, Aist, Kazuo Kobayashi, Yoshinori Kitagawa, Toshihiro Komeda, Nagako Kawashima, Yoshifumi Jigami, Yasunori Chiba filed Critical Kirin Brewery
Priority to EP03720967A priority Critical patent/EP1505149B1/fr
Priority to DE60325720T priority patent/DE60325720D1/de
Priority to JP2004501993A priority patent/JP4464269B2/ja
Priority to US10/511,436 priority patent/US7972809B2/en
Publication of WO2003091431A1 publication Critical patent/WO2003091431A1/fr
Publication of WO2003091431A8 publication Critical patent/WO2003091431A8/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/243—Colony Stimulating Factors
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/2488—Mannanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
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    • C12N9/88—Lyases (4.)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01085—3-Isopropylmalate dehydrogenase (1.1.1.85)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
    • C12Y101/03013—Alcohol oxidase (1.1.3.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01012—Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (1.2.1.12)
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    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01024—Alpha-mannosidase (3.2.1.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01019—Imidazoleglycerol-phosphate dehydratase (4.2.1.19)
    • C—CHEMISTRY; METALLURGY
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    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/02—Acid—amino-acid ligases (peptide synthases)(6.3.2)
    • C12Y603/02006—Phosphoribosylaminoimidazolesuccinocarboxamide synthase (6.3.2.6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man

Abstract

La présente invention concerne un processus de production d'une glycoprotéine comprenant une chaîne de sucre de type mammifère. Le processus de production se caractérise en ce qu'il consiste à transférer et à exprimer l'alpha-1,2-mannosidase dans le mutant du gène de la voie de biosynthèse de la chaîne sucre d'une levure de méthylotrophe sous le contrôle d'un promoteur puissant, à mettre en culture dans un milieu approprié, les cellules de levure de méthylotrophe dans lesquelles un gène étranger a été transfecté, puis à récolter dans le milieu, la glycoprotéine comprenant la chaîne de sucre de type mammifère. A l'aide d'une levure de méthylotrophe récemment cultivée portant une mutation de la chaîne de sucre, une chaîne de sucre neutre identique à une chaîne de sucre de type à forte teneur en mannose produite par des cellules de mammifère telles que des cellules humaines ou une glycoprotéine comprenant une telle chaîne de sucre neutre peut être produite, en grande quantité avec une grand niveau de pureté. Le fait de transférer un gène dans une voie de biosynthèse de la chaîne de sucre de type mammifère pour former le mutant décrit ci-avant, permet de produire efficacement une chaîne de sucre de type mammifère telle qu'une chaîne de sucre hybride ou une chaîne de sucre complexe ou bien encore une protéine comprenant une chaîne de sucre de type mammifère.
PCT/JP2003/005464 2002-04-26 2003-04-28 Methylotrophe produisant une chaine de sucre de type mammifere WO2003091431A1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP03720967A EP1505149B1 (fr) 2002-04-26 2003-04-28 Levure methylotrophe produisant une chaine de sucre de type mammifere
DE60325720T DE60325720D1 (de) 2002-04-26 2003-04-28 Methylotrophe hefe,die eine zuckerkette eines säugers herstellt
JP2004501993A JP4464269B2 (ja) 2002-04-26 2003-04-28 哺乳類型糖鎖生産メチロトロフ酵母
US10/511,436 US7972809B2 (en) 2002-04-26 2003-04-28 Methylotrophic yeast producing mammalian type sugar chain

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2002-127677 2002-04-26
JP2002127677 2002-04-26

Publications (2)

Publication Number Publication Date
WO2003091431A1 WO2003091431A1 (fr) 2003-11-06
WO2003091431A8 true WO2003091431A8 (fr) 2005-02-17

Family

ID=29267659

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2003/005464 WO2003091431A1 (fr) 2002-04-26 2003-04-28 Methylotrophe produisant une chaine de sucre de type mammifere

Country Status (6)

Country Link
US (1) US7972809B2 (fr)
EP (1) EP1505149B1 (fr)
JP (1) JP4464269B2 (fr)
AT (1) ATE420198T1 (fr)
DE (1) DE60325720D1 (fr)
WO (1) WO2003091431A1 (fr)

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Also Published As

Publication number Publication date
JP4464269B2 (ja) 2010-05-19
US7972809B2 (en) 2011-07-05
EP1505149A4 (fr) 2005-11-16
US20060148039A1 (en) 2006-07-06
DE60325720D1 (de) 2009-02-26
ATE420198T1 (de) 2009-01-15
EP1505149A1 (fr) 2005-02-09
WO2003091431A1 (fr) 2003-11-06
JPWO2003091431A1 (ja) 2005-09-02
EP1505149B1 (fr) 2009-01-07

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