WO2003080844A3 - Erhöhung des vitamin-e-gehalts in organismen durch erhöhung der 2-methyl-6-phytylhydrochinon-methyltransferase-aktivität - Google Patents

Erhöhung des vitamin-e-gehalts in organismen durch erhöhung der 2-methyl-6-phytylhydrochinon-methyltransferase-aktivität Download PDF

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Publication number
WO2003080844A3
WO2003080844A3 PCT/EP2003/002732 EP0302732W WO03080844A3 WO 2003080844 A3 WO2003080844 A3 WO 2003080844A3 EP 0302732 W EP0302732 W EP 0302732W WO 03080844 A3 WO03080844 A3 WO 03080844A3
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WO
WIPO (PCT)
Prior art keywords
phytyl
hydroquinone
methyl
organisms
vitamin
Prior art date
Application number
PCT/EP2003/002732
Other languages
English (en)
French (fr)
Other versions
WO2003080844A2 (de
Inventor
Susanne Tropf
Rainer Lemke
Klaus-Dieter Salchert
Michael Geiger
Original Assignee
Sungene Gmbh & Co Kgaa
Susanne Tropf
Rainer Lemke
Klaus-Dieter Salchert
Michael Geiger
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sungene Gmbh & Co Kgaa, Susanne Tropf, Rainer Lemke, Klaus-Dieter Salchert, Michael Geiger filed Critical Sungene Gmbh & Co Kgaa
Priority to AU2003212361A priority Critical patent/AU2003212361A1/en
Publication of WO2003080844A2 publication Critical patent/WO2003080844A2/de
Publication of WO2003080844A3 publication Critical patent/WO2003080844A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Die vorliegende Erfindung betrifft ein Verfahren zur Herstellung von Vitamin E durch Kultivierung von Organismen, insbesondere Pflanzen die gegenüber dem Wildtyp eine erhöhte 2-Methyl-6-phytylhydrochinon-Methyltransferase-Aktivität aufweisen, die Verwendung von Proteinen als 2-Methyl-6--phytylhydrochinon-Methyltransferasen, sowie die genetisch veränderten Organismen, insbesondere Pflanzen selbst.
PCT/EP2003/002732 2002-03-21 2003-03-17 Erhöhung des vitamin-e-gehalts in organismen durch erhöhung der 2-methyl-6-phytylhydrochinon-methyltransferase-aktivität WO2003080844A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003212361A AU2003212361A1 (en) 2002-03-21 2003-03-17 Enhancement of vitamin e content in organisms by enhancing 2-methyl-6-phytyl hydroquinone-methyltransferase activity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10212703.4 2002-03-21
DE10212703A DE10212703A1 (de) 2002-03-21 2002-03-21 Erhöhung Vitamin-E-Gehalts in Organismen durch Erhöhung der 2-Methyl-6-phytythydrochinon-Methyltransferase-Aktivität

Publications (2)

Publication Number Publication Date
WO2003080844A2 WO2003080844A2 (de) 2003-10-02
WO2003080844A3 true WO2003080844A3 (de) 2003-12-24

Family

ID=27798032

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/002732 WO2003080844A2 (de) 2002-03-21 2003-03-17 Erhöhung des vitamin-e-gehalts in organismen durch erhöhung der 2-methyl-6-phytylhydrochinon-methyltransferase-aktivität

Country Status (3)

Country Link
AU (1) AU2003212361A1 (de)
DE (1) DE10212703A1 (de)
WO (1) WO2003080844A2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1586645A3 (de) 1999-02-25 2006-02-22 Ceres Incorporated DNA-Fragmente mit bestimmter Sequenz und die dadurch kodierte Polypeptide
WO2006091676A2 (en) 2005-02-22 2006-08-31 Ceres Inc. Modulating plant alkaloids
WO2006133461A1 (en) 2005-06-08 2006-12-14 Ceres Inc. Identification of terpenoid-biosynthesis related regulatory protein-regulatory region associations

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1033405A2 (de) * 1999-02-25 2000-09-06 Ceres Incorporated DNS-fragmente mit bestimmter Sequenz und die dadurch kodierte Polypeptide
WO2001004330A1 (de) * 1999-07-09 2001-01-18 Sungene Gmbh & Co. Kgaa Identifizierung und überexpression einer dna-sequenz kodierend für eine 2-methyl-6-phytylhydrochinon-methyltransferase in pflanzen
WO2001062781A2 (de) * 2000-02-25 2001-08-30 Sungene Gmbh & Co. Kgaa Homogentisatphytyltransferase
WO2002000901A1 (de) * 2000-06-29 2002-01-03 Sungene Gmbh & Co. Kgaa Veränderung des gehalts an feinchemikalien in organismen durch genetische veränderung des shikimatweges

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1033405A2 (de) * 1999-02-25 2000-09-06 Ceres Incorporated DNS-fragmente mit bestimmter Sequenz und die dadurch kodierte Polypeptide
WO2001004330A1 (de) * 1999-07-09 2001-01-18 Sungene Gmbh & Co. Kgaa Identifizierung und überexpression einer dna-sequenz kodierend für eine 2-methyl-6-phytylhydrochinon-methyltransferase in pflanzen
WO2001062781A2 (de) * 2000-02-25 2001-08-30 Sungene Gmbh & Co. Kgaa Homogentisatphytyltransferase
WO2002000901A1 (de) * 2000-06-29 2002-01-03 Sungene Gmbh & Co. Kgaa Veränderung des gehalts an feinchemikalien in organismen durch genetische veränderung des shikimatweges

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE SWALL [online] BLOCK M.A. ET AL: "Molecular cloning of a cDNA encoding the precursor of the 37kDa inner envelope membrane polypeptide from tobacco chloroplasts", XP002245276, retrieved from EBI Database accession no. Q40501 *
DATABASE SWALL [online] MOTOHASHI R. ET AL: "Functional analysis of the 37kDa inner envelope membrane polypeptide in chloroplast biogenesis, using a Ds-tagged Arabidopsis pale green mutant.", XP002245275, retrieved from EBI Database accession no. Q94IE2 *
DATABASE SWALL [online] RIEGER M. ET AL: "EU Arabidopsis sequencing project.", XP002245274, retrieved from EBI Database accession no. Q9LY74 *
SHINTANI D ET AL: "ELEVATING THE VITAMIN E CONTENT OF PLANTS THROUGH METABOLIC ENGINEERING", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 282, no. 282, 1998, pages 2098 - 2100, XP000887122, ISSN: 0036-8075 *

Also Published As

Publication number Publication date
DE10212703A1 (de) 2003-10-02
AU2003212361A1 (en) 2003-10-08
WO2003080844A2 (de) 2003-10-02

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