WO2003042657A3 - Detection de sequences d'acides nucleiques par clivage et separation de structures marquees - Google Patents

Detection de sequences d'acides nucleiques par clivage et separation de structures marquees Download PDF

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Publication number
WO2003042657A3
WO2003042657A3 PCT/US2002/035552 US0235552W WO03042657A3 WO 2003042657 A3 WO2003042657 A3 WO 2003042657A3 US 0235552 W US0235552 W US 0235552W WO 03042657 A3 WO03042657 A3 WO 03042657A3
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WO
WIPO (PCT)
Prior art keywords
cleavage
detection
nucleic acid
acid sequences
tag
Prior art date
Application number
PCT/US2002/035552
Other languages
English (en)
Other versions
WO2003042657A2 (fr
Inventor
Ahmed Chenna
Vivian Xiao
Sharat Singh
Original Assignee
Aclara Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aclara Biosciences Inc filed Critical Aclara Biosciences Inc
Priority to JP2003544441A priority Critical patent/JP2005511033A/ja
Priority to CA002465588A priority patent/CA2465588A1/fr
Priority to AU2002360344A priority patent/AU2002360344A1/en
Priority to EP02795593A priority patent/EP1497455A4/fr
Publication of WO2003042657A2 publication Critical patent/WO2003042657A2/fr
Publication of WO2003042657A3 publication Critical patent/WO2003042657A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

L'invention concerne un procédé de détection de pluralités de séquences d'acides nucléiques qui consiste à former et à couper des structures duplex à l'aide de deux sondes marquées d'une étiquette électrophorétique. Le clivage des structures duplex libère les étiquettes électrophorétiques lesquelles sont ensuite séparées et identifiées pour indiquer la présence ou la quantité de séquences cibles. Cette invention est particulièrement appropriée pour les réactions multiplex dans lesquelles les séquences cibles multiples sont détectées en une seule réaction. L'invention concerne en outre des kits utilisés pour détecter les acides nucléiques.
PCT/US2002/035552 2001-11-09 2002-11-06 Detection de sequences d'acides nucleiques par clivage et separation de structures marquees WO2003042657A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2003544441A JP2005511033A (ja) 2001-11-09 2002-11-06 タグ含有構築物の切断および分離による核酸配列の決定
CA002465588A CA2465588A1 (fr) 2001-11-09 2002-11-06 Detection de sequences d'acides nucleiques par clivage et separation de structures marquees
AU2002360344A AU2002360344A1 (en) 2001-11-09 2002-11-06 Detection of nucleic acid sequences by cleavage and separation of tag-containing structures
EP02795593A EP1497455A4 (fr) 2001-11-09 2002-11-06 Detection de sequences d'acides nucleiques par clivage et separation de structures marquees

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33768601P 2001-11-09 2001-11-09
US60/337,686 2001-11-09

Publications (2)

Publication Number Publication Date
WO2003042657A2 WO2003042657A2 (fr) 2003-05-22
WO2003042657A3 true WO2003042657A3 (fr) 2004-10-28

Family

ID=23321576

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/035552 WO2003042657A2 (fr) 2001-11-09 2002-11-06 Detection de sequences d'acides nucleiques par clivage et separation de structures marquees

Country Status (5)

Country Link
EP (1) EP1497455A4 (fr)
JP (1) JP2005511033A (fr)
AU (1) AU2002360344A1 (fr)
CA (1) CA2465588A1 (fr)
WO (1) WO2003042657A2 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE44437E1 (en) 2003-04-01 2013-08-13 Monogram Biosciences, Inc. Methods for detecting receptor complexes comprising PI3K
US9110066B2 (en) 2009-01-15 2015-08-18 Laboratory Corporation Of America Holdings HER-3 antibodies and methods of use

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4742712B2 (ja) * 2005-07-14 2011-08-10 株式会社島津製作所 キャピラリー電気泳動方法
JP5143450B2 (ja) * 2007-03-14 2013-02-13 斉 大戸 Ｈｌａ−ｂローカスにおける新規アリル
CA2711843C (fr) 2007-12-20 2018-11-13 Laboratory Corporation Of America Holdings Procedes de diagnostic du her-2
SG177252A1 (en) 2008-12-01 2012-03-29 Lab Corp America Holdings METHODS AND ASSAYS FOR MEASURING p95 AND/OR p95 IN A SAMPLE AND ANTIBODIES SPECIFIC FOR p95
US10526641B2 (en) 2014-08-01 2020-01-07 Dovetail Genomics, Llc Tagging nucleic acids for sequence assembly
WO2016134034A1 (fr) 2015-02-17 2016-08-25 Dovetail Genomics Llc Assemblage de séquences d'acide nucléique
US11807896B2 (en) 2015-03-26 2023-11-07 Dovetail Genomics, Llc Physical linkage preservation in DNA storage
SG11201803289VA (en) 2015-10-19 2018-05-30 Dovetail Genomics Llc Methods for genome assembly, haplotype phasing, and target independent nucleic acid detection
EP3417059A4 (fr) * 2016-02-20 2019-10-30 Vladimir I. Bashkirov Procédés et systèmes pour la détection d'acides nucléiques cibles
KR20180116377A (ko) 2016-02-23 2018-10-24 더브테일 제노믹스 엘엘씨 게놈 어셈블리를 위한 페이징된 판독 세트의 생성 및 반수체형 페이징
CA3016914C (fr) 2016-03-15 2019-08-27 Laboratory Corporation Of America Holdings Procedes d'evaluation d'interactions de proteines entre des cellules
EP3455356B1 (fr) 2016-05-13 2021-08-04 Dovetail Genomics LLC Récupération d'informations de liaison de longue portée à partir d'échantillons conservés
CN109946360B (zh) * 2019-03-11 2022-01-21 中国科学院苏州生物医学工程技术研究所 检测8-羟基鸟嘌呤dna糖苷酶的传感器及方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5449602A (en) * 1988-01-13 1995-09-12 Amoco Corporation Template-directed photoligation
US5843650A (en) * 1995-05-01 1998-12-01 Segev; David Nucleic acid detection and amplification by chemical linkage of oligonucleotides

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6322980B1 (en) * 1999-04-30 2001-11-27 Aclara Biosciences, Inc. Single nucleotide detection using degradation of a fluorescent sequence

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5449602A (en) * 1988-01-13 1995-09-12 Amoco Corporation Template-directed photoligation
US5843650A (en) * 1995-05-01 1998-12-01 Segev; David Nucleic acid detection and amplification by chemical linkage of oligonucleotides

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1497455A4 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE44437E1 (en) 2003-04-01 2013-08-13 Monogram Biosciences, Inc. Methods for detecting receptor complexes comprising PI3K
US9110066B2 (en) 2009-01-15 2015-08-18 Laboratory Corporation Of America Holdings HER-3 antibodies and methods of use

Also Published As

Publication number Publication date
EP1497455A2 (fr) 2005-01-19
CA2465588A1 (fr) 2003-05-22
EP1497455A4 (fr) 2005-12-28
AU2002360344A1 (en) 2003-05-26
WO2003042657A2 (fr) 2003-05-22
JP2005511033A (ja) 2005-04-28

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