CA2465588A1 - Detection de sequences d'acides nucleiques par clivage et separation de structures marquees - Google Patents

Detection de sequences d'acides nucleiques par clivage et separation de structures marquees Download PDF

Info

Publication number
CA2465588A1
CA2465588A1 CA002465588A CA2465588A CA2465588A1 CA 2465588 A1 CA2465588 A1 CA 2465588A1 CA 002465588 A CA002465588 A CA 002465588A CA 2465588 A CA2465588 A CA 2465588A CA 2465588 A1 CA2465588 A1 CA 2465588A1
Authority
CA
Canada
Prior art keywords
electrophoretic
probe
probes
tag
tags
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002465588A
Other languages
English (en)
Inventor
Ahmed Chenna
Vivian Xiao
Sharat Singh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monogram Biosciences Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2465588A1 publication Critical patent/CA2465588A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

L'invention concerne un procédé de détection de pluralités de séquences d'acides nucléiques qui consiste à former et à couper des structures duplex à l'aide de deux sondes marquées d'une étiquette électrophorétique. Le clivage des structures duplex libère les étiquettes électrophorétiques lesquelles sont ensuite séparées et identifiées pour indiquer la présence ou la quantité de séquences cibles. Cette invention est particulièrement appropriée pour les réactions multiplex dans lesquelles les séquences cibles multiples sont détectées en une seule réaction. L'invention concerne en outre des kits utilisés pour détecter les acides nucléiques.
CA002465588A 2001-11-09 2002-11-06 Detection de sequences d'acides nucleiques par clivage et separation de structures marquees Abandoned CA2465588A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US33768601P 2001-11-09 2001-11-09
US60/337,686 2001-11-09
PCT/US2002/035552 WO2003042657A2 (fr) 2001-11-09 2002-11-06 Detection de sequences d'acides nucleiques par clivage et separation de structures marquees

Publications (1)

Publication Number Publication Date
CA2465588A1 true CA2465588A1 (fr) 2003-05-22

Family

ID=23321576

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002465588A Abandoned CA2465588A1 (fr) 2001-11-09 2002-11-06 Detection de sequences d'acides nucleiques par clivage et separation de structures marquees

Country Status (5)

Country Link
EP (1) EP1497455A4 (fr)
JP (1) JP2005511033A (fr)
AU (1) AU2002360344A1 (fr)
CA (1) CA2465588A1 (fr)
WO (1) WO2003042657A2 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040229294A1 (en) 2002-05-21 2004-11-18 Po-Ying Chan-Hui ErbB surface receptor complexes as biomarkers
JP4742712B2 (ja) * 2005-07-14 2011-08-10 株式会社島津製作所 キャピラリー電気泳動方法
JP5143450B2 (ja) * 2007-03-14 2013-02-13 斉 大戸 Ｈｌａ−ｂローカスにおける新規アリル
CA2711843C (fr) 2007-12-20 2018-11-13 Laboratory Corporation Of America Holdings Procedes de diagnostic du her-2
CA2764386C (fr) 2008-12-01 2018-05-01 Laboratory Corporation Of America Holdings Anticorps p35-her2 et leurs utilisations
SG172983A1 (en) 2009-01-15 2011-08-29 Lab Corp America Holdings Methods of determining patient response by measurement of her-3
CA2956925C (fr) 2014-08-01 2024-02-13 Dovetail Genomics, Llc Marquage d'acides nucleiques pour l'assemblage de sequences
SG10202000731WA (en) 2015-02-17 2020-03-30 Dovetail Genomics Llc Nucleic acid sequence assembly
WO2016154540A1 (fr) 2015-03-26 2016-09-29 Dovetail Genomics Llc Conservation de la liaison physique dans le stockage d'adn
AU2016341198B2 (en) * 2015-10-19 2023-03-09 Dovetail Genomics, Llc Methods for genome assembly, haplotype phasing, and target independent nucleic acid detection
WO2017143317A1 (fr) * 2016-02-20 2017-08-24 Bashkirov Vladimir I Procédés et systèmes pour la détection d'acides nucléiques cibles
CA3014911A1 (fr) 2016-02-23 2017-08-31 Dovetail Genomics, Llc Production d'ensembles de lecture en phase servant a l'assemblage du genome et la mise en phase d'haplotype
US10451614B2 (en) 2016-03-15 2019-10-22 Laboratory Corporation Of America Holdings Methods of assessing protein interactions between cells
AU2017263810B2 (en) 2016-05-13 2023-08-17 Dovetail Genomics Llc Recovering long-range linkage information from preserved samples
CN109946360B (zh) * 2019-03-11 2022-01-21 中国科学院苏州生物医学工程技术研究所 检测8-羟基鸟嘌呤dna糖苷酶的传感器及方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5449602A (en) * 1988-01-13 1995-09-12 Amoco Corporation Template-directed photoligation
US5843650A (en) * 1995-05-01 1998-12-01 Segev; David Nucleic acid detection and amplification by chemical linkage of oligonucleotides
US6322980B1 (en) * 1999-04-30 2001-11-27 Aclara Biosciences, Inc. Single nucleotide detection using degradation of a fluorescent sequence

Also Published As

Publication number Publication date
EP1497455A4 (fr) 2005-12-28
AU2002360344A1 (en) 2003-05-26
JP2005511033A (ja) 2005-04-28
WO2003042657A2 (fr) 2003-05-22
WO2003042657A3 (fr) 2004-10-28
EP1497455A2 (fr) 2005-01-19

Similar Documents

Publication Publication Date Title
US20060223107A1 (en) Detection of nucleic acid sequences by cleavage and separation of tag-containing structures
AU2002324825B2 (en) Terminal-phosphate-labeled nucleotides and methods of use
Balintová et al. Anthraquinone as a redox label for DNA: synthesis, enzymatic incorporation, and electrochemistry of anthraquinone‐modified nucleosides, nucleotides, and DNA
CN101384729B (zh) 固相测序
US7405281B2 (en) Fluorescent nucleotide analogs and uses therefor
CA2513672C (fr) Nucleotides marques sur le phosphate de terminaison et methodes d'utilisation
US5728528A (en) Universal spacer/energy transfer dyes
CA2465588A1 (fr) Detection de sequences d'acides nucleiques par clivage et separation de structures marquees
AU2002324825A1 (en) Terminal-phosphate-labeled nucleotides and methods of use
EP1581796A2 (fr) Plate-forme analytique multiplexee utilisant des etiquettes moleculaires
EP3452219A1 (fr) Séquençage d'adn sur molécule unique/ensemble basé sur le transfert d'énergie de fluorescence par synthèse
KR20170039743A (ko) 변형된 뉴클레오타이드 링커
CA2368581A1 (fr) Detection utilisant la degradation d'une sequence marquee
JPH03502041A (ja) Ｄｎａおよびｒｎａの迅速塩基配列決定方法
EP0832286A1 (fr) Nucleotides modifies marqueurs d'acides nucleiques
CN102083846A (zh) 包含2’-终止子核苷酸的核酸的合成和组合物
US20050053939A1 (en) Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents
WO2003042658A2 (fr) Procedes et compositions permettant d'ameliorer la detection dans des analyses dans lesquelles on utilise des reactifs a marqueurs electrophoretiques clivables
EP1112281B1 (fr) Analogues de pteridines nucleotidiques
JP4738741B2 (ja) テンプレートされた分子を合成するための改良法
JP2005521365A6 (ja) テンプレートされた分子を合成するための改良法

Legal Events

Date Code Title Description
FZDE Dead