WO2003022043A1 - Systeme de reconstitution de follicule pileux et animal portant ce systeme - Google Patents
Systeme de reconstitution de follicule pileux et animal portant ce systeme Download PDFInfo
- Publication number
- WO2003022043A1 WO2003022043A1 PCT/JP2002/009082 JP0209082W WO03022043A1 WO 2003022043 A1 WO2003022043 A1 WO 2003022043A1 JP 0209082 W JP0209082 W JP 0209082W WO 03022043 A1 WO03022043 A1 WO 03022043A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- cells
- hair
- dermal
- mouse
- animal
- Prior art date
Links
- 241001465754 Metazoa Species 0.000 title claims abstract description 84
- 210000004209 hair Anatomy 0.000 title claims abstract description 54
- 210000004027 cell Anatomy 0.000 claims abstract description 93
- 210000003780 hair follicle Anatomy 0.000 claims abstract description 55
- 210000001339 epidermal cell Anatomy 0.000 claims abstract description 38
- 210000002752 melanocyte Anatomy 0.000 claims abstract description 31
- 241000699666 Mus <mouse, genus> Species 0.000 claims description 43
- 230000002500 effect on skin Effects 0.000 claims description 39
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 claims description 26
- 241000699670 Mus sp. Species 0.000 claims description 20
- 229940079593 drug Drugs 0.000 claims description 19
- 239000003814 drug Substances 0.000 claims description 19
- 230000000694 effects Effects 0.000 claims description 19
- 210000004927 skin cell Anatomy 0.000 claims description 19
- 238000000034 method Methods 0.000 claims description 17
- 210000003491 skin Anatomy 0.000 claims description 15
- 238000011580 nude mouse model Methods 0.000 claims description 10
- 238000011156 evaluation Methods 0.000 claims description 9
- 238000012360 testing method Methods 0.000 claims description 9
- 230000002950 deficient Effects 0.000 claims description 8
- 210000002615 epidermis Anatomy 0.000 claims description 7
- 230000008859 change Effects 0.000 claims description 6
- 239000000049 pigment Substances 0.000 claims description 6
- 210000000987 immune system Anatomy 0.000 claims description 4
- 241000894007 species Species 0.000 claims description 4
- 230000004913 activation Effects 0.000 claims description 3
- 238000012544 monitoring process Methods 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 2
- 210000004694 pigment cell Anatomy 0.000 claims 2
- 239000004480 active ingredient Substances 0.000 claims 1
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- 230000037308 hair color Effects 0.000 abstract description 12
- 230000003779 hair growth Effects 0.000 abstract description 9
- 238000001356 surgical procedure Methods 0.000 description 11
- 241000699660 Mus musculus Species 0.000 description 9
- 210000004207 dermis Anatomy 0.000 description 9
- 108090000623 proteins and genes Proteins 0.000 description 9
- 238000012216 screening Methods 0.000 description 8
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 6
- 238000011830 transgenic mouse model Methods 0.000 description 6
- 102000004142 Trypsin Human genes 0.000 description 5
- 108090000631 Trypsin Proteins 0.000 description 5
- 230000003213 activating effect Effects 0.000 description 5
- 239000006285 cell suspension Substances 0.000 description 5
- 239000012588 trypsin Substances 0.000 description 5
- ALYNCZNDIQEVRV-UHFFFAOYSA-N 4-aminobenzoic acid Chemical compound NC1=CC=C(C(O)=O)C=C1 ALYNCZNDIQEVRV-UHFFFAOYSA-N 0.000 description 4
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- 238000002360 preparation method Methods 0.000 description 4
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- 102000029816 Collagenase Human genes 0.000 description 3
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- 238000002054 transplantation Methods 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 2
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 2
- 108700008625 Reporter Genes Proteins 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 210000000981 epithelium Anatomy 0.000 description 2
- 239000005090 green fluorescent protein Substances 0.000 description 2
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- 230000006698 induction Effects 0.000 description 2
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- 102000005600 Cathepsins Human genes 0.000 description 1
- 108010084457 Cathepsins Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 206010039509 Scab Diseases 0.000 description 1
- 102000003425 Tyrosinase Human genes 0.000 description 1
- 108060008724 Tyrosinase Proteins 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 229960004050 aminobenzoic acid Drugs 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229940035674 anesthetics Drugs 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000000386 athletic effect Effects 0.000 description 1
- 210000000270 basal cell Anatomy 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
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- 210000002808 connective tissue Anatomy 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 210000000185 follicular epithelial cell Anatomy 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000002825 functional assay Methods 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 239000003193 general anesthetic agent Substances 0.000 description 1
- 230000009395 genetic defect Effects 0.000 description 1
- 210000002768 hair cell Anatomy 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
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- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 238000002278 reconstructive surgery Methods 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 210000004918 root sheath Anatomy 0.000 description 1
- 210000001732 sebaceous gland Anatomy 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 230000003797 telogen phase Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
- C12N5/12—Fused cells, e.g. hybridomas
- C12N5/16—Animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
- C12N5/0698—Skin equivalents
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
- C12N5/0626—Melanocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
- C12N5/0627—Hair cells
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/105—Murine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/03—Animal model, e.g. for test or diseases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
- C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1323—Adult fibroblasts
Definitions
- the present inventors have studied to achieve the above-mentioned object, and it has been confirmed that the above-mentioned hair follicles are reconstructed.
- the so-called reconstructed hair follicles are obtained by adding exogenous melanosites of the same type or different types. Can be reconfigured, and in this way It has been found that the formed melanosite of the hair follicle can be kept active. Therefore, according to the present invention, there is provided a system for reconstituting a hair follicle composed of a combination of hair papilla cells and epidermal cells and foreign melanocytes for these cells.
- the above-mentioned dermal papilla cells and epidermal cells are preferably derived from animals of the same species as the recipient animal.
- specific examples of such animals include mouse And rat.
- the “system for reconstituting the hair follicle” of the present invention comprises a combination of the above-mentioned hair papilla cells and epidermal cells, and an exogenous melanocyte.
- a “combination system” includes not only the case where the above-mentioned cells are integrally contained, but also the case where individual cells are stored individually for the purpose of using them in the future. This has the meaning of including the form contained in the container. Thus, they are within the scope of the present invention as long as they are used for the purpose of reconstituting the above system.
- Chimeric animals carrying the reconstituted hair follicles as described above can induce long-term melanin-containing hair from the reconstituted hair follicles and grow them, and are therefore involved in hair growth and darkening of hair color.
- Cells or tissues or organs, such as melanosites, dermal papilla, or hair matrix cells, are considered to be working normally. Therefore, the chimeric animal according to the present invention
- Any or all of the above-mentioned actions can be used for screening drugs having a hair coloring action and drugs having a combination action thereof.
- the range of drugs or chemicals for activating melanosite according to the present invention includes any one of growth, differentiation, proliferation, survival, and athletic ability of melanosite, or some or all of the activities. Drugs that have the effect of deepening hair color by stimulating and activating hair, including those whose activity is indicated by hair color.
- step (B) treating the test animal by a certain means, and monitoring the activity of melanosite in the reconstituted hair follicle of the test animal thus treated;
- C) a non-treated subject for example, the step (B) (B) the degree of change in the activity of step (B) with respect to the reconstituted hair follicles in the reconstructed hair follicle of the test animal not subjected to the treatment in step (B), and the ability of the means to activate the melanocyte.
- the method for evaluating the activating ability of melanocytes comprising by such an evaluation method, the activation ability of melanocyte can be evaluated by the amount of melanin pigment in hair growth from reconstructed hair follicles. In addition, depending on the degree of hair growth and hair growth, either one or both of the hair papilla and hair matrix
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Dermatology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Animal Behavior & Ethology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Cosmetics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP02767910A EP1437042A4 (en) | 2001-09-06 | 2002-09-06 | CELL AND ANIMAL FOLLICLE RECONSTRUCTION SYSTEM CARRYING OUT THIS SYSTEM |
KR10-2004-7002913A KR20040029451A (ko) | 2001-09-06 | 2002-09-06 | 모포 재구성계 및 그 담지 동물 |
US10/488,708 US20040247572A1 (en) | 2001-09-06 | 2002-09-06 | Hair follicle-reconstitution system and animal carrying the same |
US11/905,595 US20080109915A1 (en) | 2001-09-06 | 2007-10-02 | Hair follicle-reconstitution system and animal carrying the same |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2001-270100 | 2001-09-06 | ||
JP2001270100 | 2001-09-06 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US11/905,595 Division US20080109915A1 (en) | 2001-09-06 | 2007-10-02 | Hair follicle-reconstitution system and animal carrying the same |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2003022043A1 true WO2003022043A1 (fr) | 2003-03-20 |
Family
ID=19095799
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2002/009082 WO2003022043A1 (fr) | 2001-09-06 | 2002-09-06 | Systeme de reconstitution de follicule pileux et animal portant ce systeme |
Country Status (5)
Country | Link |
---|---|
US (2) | US20040247572A1 (ja) |
EP (1) | EP1437042A4 (ja) |
KR (1) | KR20040029451A (ja) |
CN (1) | CN1297320C (ja) |
WO (1) | WO2003022043A1 (ja) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006057542A1 (en) * | 2004-11-29 | 2006-06-01 | Lifecord International Co., Ltd. | Method for the preparation of a dermal papilla tissue having hair follicle inductive potency |
WO2012115079A1 (ja) * | 2011-02-24 | 2012-08-30 | 株式会社オーガンテクノロジーズ | 毛色が制御された移植用再生毛包原基の製造方法、当該移植用再生毛包原基を含む組成物、およびその移植方法 |
US9023380B2 (en) | 2005-11-22 | 2015-05-05 | Aderans Research Institute, Inc. | Hair follicle graft from tissue engineered skin |
CN104877953A (zh) * | 2014-02-28 | 2015-09-02 | 上海尚瑞生物医药科技有限公司 | 促进黑色素细胞色素产生的皮肤制剂 |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005033302A1 (ja) * | 2003-10-06 | 2005-04-14 | Shiseido Company, Ltd. | 毛乳頭細胞調製品の調製方法、毛包を再生するための組成物、方法及び再生された毛包を担持する動物 |
CN100362972C (zh) * | 2004-12-24 | 2008-01-23 | 李宇 | 人工毛乳头替代结构及其制作方法 |
MX2008004928A (es) * | 2005-10-17 | 2008-09-12 | Aderans Res Inst Inc | Metodo para suministrar celulas progenitoras de foliculo piloso a la piel. |
GB0605450D0 (en) | 2006-03-17 | 2006-04-26 | Intercytex Ltd | Cell co-culture |
US7985537B2 (en) | 2007-06-12 | 2011-07-26 | Aderans Research Institute, Inc. | Methods for determining the hair follicle inductive properties of a composition |
IN2013MN00607A (ja) * | 2010-09-29 | 2015-09-11 | Shiseido Co Ltd | |
CN103785064B (zh) * | 2013-08-26 | 2015-02-25 | 济南磐升生物技术有限公司 | 一种皮肤模型及应用 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999045770A1 (en) * | 1998-03-09 | 1999-09-16 | The Board Of Trustees Of The Leland Stanford Junior University | Skin equivalent and methods of forming and using same |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5352440A (en) * | 1988-03-30 | 1994-10-04 | Trustees Of Boston University | Methods for increasing melanin content in melanocytes using diacylglycerols and uses thereof |
US5885822A (en) * | 1996-08-20 | 1999-03-23 | David A. Paslin | Method and system for growing molluscum contagiosum in xenografts to immunocompromised hosts |
GB9925964D0 (en) * | 1999-11-03 | 1999-12-29 | Jahoda Colin A B | Hair transplantation |
-
2002
- 2002-09-06 KR KR10-2004-7002913A patent/KR20040029451A/ko not_active Application Discontinuation
- 2002-09-06 CN CNB028172442A patent/CN1297320C/zh not_active Expired - Fee Related
- 2002-09-06 EP EP02767910A patent/EP1437042A4/en not_active Ceased
- 2002-09-06 WO PCT/JP2002/009082 patent/WO2003022043A1/ja active Application Filing
- 2002-09-06 US US10/488,708 patent/US20040247572A1/en not_active Abandoned
-
2007
- 2007-10-02 US US11/905,595 patent/US20080109915A1/en not_active Abandoned
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999045770A1 (en) * | 1998-03-09 | 1999-09-16 | The Board Of Trustees Of The Leland Stanford Junior University | Skin equivalent and methods of forming and using same |
Non-Patent Citations (4)
Title |
---|
KAMIMURA J. ET AL.: "Primary mouse keratinocyte cultures contain hair follicle progenitor cells with multiple differentiation potential", J. INVEST. DERMATOL., vol. 109, no. 4, 1997, pages 534 - 540, XP002959862 * |
ROSENBERG A.S. ET AL.: "Evidence that the effector mechanism of skin allograft rejection is antigen-specific", PROC. NATL. ACAD. SCI. USA, vol. 85, no. 20, 1988, pages 7739 - 7742, XP002959861 * |
See also references of EP1437042A4 * |
TOBIN D.J. ET AL.: "Isolation and long-term culture of human hair-follicle melanocytes", J. INVEST. DERMATOL., vol. 104, no. 1, 1995, pages 86 - 89, XP002959863 * |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006057542A1 (en) * | 2004-11-29 | 2006-06-01 | Lifecord International Co., Ltd. | Method for the preparation of a dermal papilla tissue having hair follicle inductive potency |
US7635589B2 (en) | 2004-11-29 | 2009-12-22 | Lifecord Inc., Corp. | Method for the preparation of a dermal papilla tissue having hair follicle inductive potency |
US9023380B2 (en) | 2005-11-22 | 2015-05-05 | Aderans Research Institute, Inc. | Hair follicle graft from tissue engineered skin |
WO2012115079A1 (ja) * | 2011-02-24 | 2012-08-30 | 株式会社オーガンテクノロジーズ | 毛色が制御された移植用再生毛包原基の製造方法、当該移植用再生毛包原基を含む組成物、およびその移植方法 |
JP5887333B2 (ja) * | 2011-02-24 | 2016-03-16 | 株式会社オーガンテクノロジーズ | 毛色が制御された移植用再生毛包原基の製造方法、当該移植用再生毛包原基を含む組成物、およびその移植方法 |
US9321998B2 (en) | 2011-02-24 | 2016-04-26 | Organ Technologies Inc. | Method of preparing regenerated hair follicle germ for transplantation in which hair color is controlled, composition including regenerated hair follicle germ for transplantation, and method of transplanting regenerated hair follicle germ |
KR101854490B1 (ko) | 2011-02-24 | 2018-05-03 | 가부시키가이샤 오간 테크놀로지즈 | 모색이 제어된 이식용 재생 모낭 원기의 제조방법, 당해 이식용 재생 모낭 원기를 포함하는 조성물 및 그의 이식방법 |
CN104877953A (zh) * | 2014-02-28 | 2015-09-02 | 上海尚瑞生物医药科技有限公司 | 促进黑色素细胞色素产生的皮肤制剂 |
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EP1437042A1 (en) | 2004-07-14 |
CN1551726A (zh) | 2004-12-01 |
US20080109915A1 (en) | 2008-05-08 |
US20040247572A1 (en) | 2004-12-09 |
EP1437042A4 (en) | 2006-05-03 |
CN1297320C (zh) | 2007-01-31 |
KR20040029451A (ko) | 2004-04-06 |
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