WO2003006497A3 - Photoprotéines mutees et leurs applications - Google Patents

Photoprotéines mutees et leurs applications Download PDF

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Publication number
WO2003006497A3
WO2003006497A3 PCT/FR2002/002492 FR0202492W WO03006497A3 WO 2003006497 A3 WO2003006497 A3 WO 2003006497A3 FR 0202492 W FR0202492 W FR 0202492W WO 03006497 A3 WO03006497 A3 WO 03006497A3
Authority
WO
WIPO (PCT)
Prior art keywords
photoproteins
mutated
derived
methods
detecting
Prior art date
Application number
PCT/FR2002/002492
Other languages
English (en)
Other versions
WO2003006497A2 (fr
Inventor
Bertrand Lambolez
Nathalie Gibelin
Gaelle Bourout
Ludovic Eric Tricoire
Olivier Arsene Courjean
Keisuke Tsuzuki
Jean Rossier
Original Assignee
Centre Nat Rech Scient
Bertrand Lambolez
Nathalie Gibelin
Gaelle Bourout
Ludovic Eric Tricoire
Olivier Arsene Courjean
Keisuke Tsuzuki
Jean Rossier
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre Nat Rech Scient, Bertrand Lambolez, Nathalie Gibelin, Gaelle Bourout, Ludovic Eric Tricoire, Olivier Arsene Courjean, Keisuke Tsuzuki, Jean Rossier filed Critical Centre Nat Rech Scient
Priority to EP02784875A priority Critical patent/EP1404711A2/fr
Priority to AU2002333989A priority patent/AU2002333989A1/en
Priority to CA002455542A priority patent/CA2455542A1/fr
Priority to US10/484,380 priority patent/US20050130262A1/en
Publication of WO2003006497A2 publication Critical patent/WO2003006497A2/fr
Publication of WO2003006497A3 publication Critical patent/WO2003006497A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention a pour objet des photoprotéines mutées dérivées des photoprotéines isolées de méduses, lesdites photoprotéines mutées étant caractérisées par une thermostabilité superieure à celle des photoprotéines dont elles dérivent, et sont désignées photoprotéines mutées thermostables, et/ou par un temps de luminescence supérieur à celui des photoprotéines dont elles dérivent, et sont désignées photoprotéines mutées persistantes. L'invention concerne également l'utilisation des photoprotéines mutées susmentionnées dans le cadre de la mise en oeuvre de procédés de détection in vitro de molécules dans un échantillon biologique, de procédés de detection de composes à activité enzymatique dans un échantillon biologique, ou de procédés de détection des variations de calcium intracellulaire.
PCT/FR2002/002492 2001-07-12 2002-07-12 Photoprotéines mutees et leurs applications WO2003006497A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP02784875A EP1404711A2 (fr) 2001-07-12 2002-07-12 Photoproteines mutees et leurs applications
AU2002333989A AU2002333989A1 (en) 2001-07-12 2002-07-12 Mutated photoproteins and their uses
CA002455542A CA2455542A1 (fr) 2001-07-12 2002-07-12 Photoproteines mutees et leurs applications
US10/484,380 US20050130262A1 (en) 2001-07-12 2002-07-12 Mutated photoproteins and their uses

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0109293A FR2827292B1 (fr) 2001-07-12 2001-07-12 Photoproteines mutees et leurs applications
FR01/09293 2001-07-12

Publications (2)

Publication Number Publication Date
WO2003006497A2 WO2003006497A2 (fr) 2003-01-23
WO2003006497A3 true WO2003006497A3 (fr) 2004-01-22

Family

ID=8865426

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2002/002492 WO2003006497A2 (fr) 2001-07-12 2002-07-12 Photoprotéines mutees et leurs applications

Country Status (6)

Country Link
US (1) US20050130262A1 (fr)
EP (1) EP1404711A2 (fr)
AU (1) AU2002333989A1 (fr)
CA (1) CA2455542A1 (fr)
FR (1) FR2827292B1 (fr)
WO (1) WO2003006497A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10328067A1 (de) * 2003-06-23 2005-01-27 Bayer Healthcare Ag Isoliertes Photoprotein Bolinopsin, sowie dessen Verwendung
DE10342670A1 (de) * 2003-09-16 2005-04-21 Bayer Healthcare Ag Isoliertes Photoprotein mtClytin, sowie dessen Verwendung
EP1700865A1 (fr) * 2005-03-11 2006-09-13 AXXAM S.r.l. Photoprotéines ayant une bioluminescence améliorée et leur utilisation comme indicateurs de calcium intracellulaire
DE102005022146A1 (de) * 2005-05-13 2006-11-23 Bayer Healthcare Ag Isoliertes Photoprotein AQdecay sowie dessen Verwendung
JP5374840B2 (ja) 2006-07-20 2013-12-25 Ｊｎｃ株式会社 カルシウム結合発光蛋白質、それをコードする遺伝子およびその用途
DE102007011241A1 (de) 2007-03-08 2008-09-11 Bayer Healthcare Ag Isoliertes Photoprotein mtClytinDecay, sowie dessen Verwendung
JP2010143860A (ja) * 2008-12-19 2010-07-01 Chisso Corp タンパク質の安定化剤
JP5549113B2 (ja) * 2009-05-14 2014-07-16 Ｊｎｃ株式会社 カルシウム結合発光蛋白質、それをコードする遺伝子およびその用途
WO2011008250A1 (fr) * 2009-07-14 2011-01-20 Millipore Corporation Photoprotéines modifiées présentant une affinité accrue pour le calcium, ainsi qu'une bioluminescence renforcée, et leurs utilisations
CN116143893B (zh) * 2022-08-09 2023-11-10 无锡佰翱得生物科学股份有限公司 一种增强型单体StayGold蛋白及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5541309A (en) * 1992-12-01 1996-07-30 Woods Hole Oceanographic Institution (Whoi) Modified apoaequorin having increased bioluminescent activity
JPH09278796A (ja) * 1996-04-16 1997-10-28 Mochida Pharmaceut Co Ltd 熱安定性が増強したエクオリン改変体
JP2001270899A (ja) * 2000-03-24 2001-10-02 Chisso Corp 発光量標準物質

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7345160B2 (en) * 2004-03-29 2008-03-18 University Of Kentucky Research Foundation Aequorin and obelin mutants with differing wavelengths and bioluminescence

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5541309A (en) * 1992-12-01 1996-07-30 Woods Hole Oceanographic Institution (Whoi) Modified apoaequorin having increased bioluminescent activity
JPH09278796A (ja) * 1996-04-16 1997-10-28 Mochida Pharmaceut Co Ltd 熱安定性が増強したエクオリン改変体
JP2001270899A (ja) * 2000-03-24 2001-10-02 Chisso Corp 発光量標準物質

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BALDWIN T O ET AL.: "The complete nucleotide sequence of the lux regulon of Vibrio fischeri and the luxABN region of Photobacterium leiognathi and the mechanism of control of bacterial bioluminescence", JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE, vol. 4, no. 1, 1989, pages 326 - 341, XP001064768 *
DATABASE CA [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; 26 January 1998 (1998-01-26), MOCHIZUKI, HIROSHI ET AL: "Preparation of aequorin derivatives with improved stability", XP002198117, Database accession no. 128:32705 *
DATABASE REGISTRY "Aequorin [59-arginine] (Aequorea victoria)", XP002198118 *
DATABASE WPI Section Ch Week 200211, Derwent World Patents Index; Class B04, AN 2002-078318, XP002198119 *
OHMIYA YOSHIHIRO ET AL: "Bioluminescence of the calcium binding photoprotein, aequorin, after histidine modification.", FEBS (FEDERATION OF EUROPEAN BIOCHEMICAL SOCIETIES) LETTERS, vol. 320, no. 3, 1993, pages 267 - 270, XP002198116, ISSN: 0014-5793 *
TSUJI F I ET AL: "SITE-SPECIFIC MUTAGENESIS OF THE CALCIUM-BINDING PHOTOPROTEIN AEQUORIN", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 83, no. 21, 1986, 1986, pages 8107 - 8111, XP002198115, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
FR2827292B1 (fr) 2004-06-18
WO2003006497A2 (fr) 2003-01-23
US20050130262A1 (en) 2005-06-16
AU2002333989A1 (en) 2003-01-29
FR2827292A1 (fr) 2003-01-17
EP1404711A2 (fr) 2004-04-07
CA2455542A1 (fr) 2003-01-23

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