DE60043937D1 - Eine in vivo "bibliothek-gegen-bibliothek-selektion" von optimierten protein-protein-wechselwirkungen - Google Patents

Eine in vivo "bibliothek-gegen-bibliothek-selektion" von optimierten protein-protein-wechselwirkungen

Info

Publication number
DE60043937D1
DE60043937D1 DE60043937T DE60043937T DE60043937D1 DE 60043937 D1 DE60043937 D1 DE 60043937D1 DE 60043937 T DE60043937 T DE 60043937T DE 60043937 T DE60043937 T DE 60043937T DE 60043937 D1 DE60043937 D1 DE 60043937D1
Authority
DE
Germany
Prior art keywords
library
protein
pairs
selection
vivo
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60043937T
Other languages
English (en)
Inventor
Stephen W Michnick
Joelle Nina Pelletier
Andreas Pluckthun
Katja M Arndt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Odyssey Pharmaceuticals Inc
Original Assignee
Odyssey Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Odyssey Pharmaceuticals Inc filed Critical Odyssey Pharmaceuticals Inc
Priority claimed from US09/603,885 external-priority patent/US6897017B1/en
Application granted granted Critical
Publication of DE60043937D1 publication Critical patent/DE60043937D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE60043937T 1999-06-26 2000-06-26 Eine in vivo "bibliothek-gegen-bibliothek-selektion" von optimierten protein-protein-wechselwirkungen Expired - Fee Related DE60043937D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14121099P 1999-06-26 1999-06-26
PCT/US2000/017436 WO2001000866A1 (en) 1999-06-26 2000-06-26 An in vivo library-versus-library selection of optimized protein-protein interactions
US09/603,885 US6897017B1 (en) 1997-01-31 2000-06-26 Vivo library-versus-library selection of optimized protein-protein interactions

Publications (1)

Publication Number Publication Date
DE60043937D1 true DE60043937D1 (de) 2010-04-15

Family

ID=22494676

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60043937T Expired - Fee Related DE60043937D1 (de) 1999-06-26 2000-06-26 Eine in vivo "bibliothek-gegen-bibliothek-selektion" von optimierten protein-protein-wechselwirkungen

Country Status (7)

Country Link
EP (1) EP1198586B1 (de)
JP (1) JP2003503067A (de)
AT (1) ATE459724T1 (de)
AU (1) AU6054900A (de)
CA (1) CA2377573A1 (de)
DE (1) DE60043937D1 (de)
WO (1) WO2001000866A1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001001137A1 (en) 1999-06-30 2001-01-04 Children's Medical Center Corporation Fusion protein and uses thereof
CA2384561A1 (en) 2000-05-12 2001-11-22 Yale University Methods of detecting interactions between proteins, peptides or libraries thereof using fusion proteins
WO2001098534A2 (en) * 2000-06-23 2001-12-27 Domantis Limited Matrix screening method
US20020115068A1 (en) * 2000-06-23 2002-08-22 Ian Tomlinson Matrix screening method
EP2075256A2 (de) 2002-01-14 2009-07-01 William Herman Gezielte Liganden
KR101150334B1 (ko) * 2011-02-08 2012-06-08 엘에스산전 주식회사 진공차단기의 플렉서블 션트

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992020791A1 (en) 1990-07-10 1992-11-26 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
EP1405911A1 (de) * 1994-07-20 2004-04-07 The General Hospital Corporation System zur Erkennung von Protein-Interaktionen
US5610015A (en) * 1995-03-23 1997-03-11 Wisconsin Alumni Research Foundation System to detect protein-RNA interactions
GB9712818D0 (en) * 1996-07-08 1997-08-20 Cambridge Antibody Tech Labelling and selection of specific binding molecules
US5925523A (en) * 1996-08-23 1999-07-20 President & Fellows Of Harvard College Intraction trap assay, reagents and uses thereof
CA2196496A1 (en) * 1997-01-31 1998-07-31 Stephen William Watson Michnick Protein fragment complementation assay for the detection of protein-protein interactions
JP2002526756A (ja) * 1998-09-24 2002-08-20 デューク ユニバーシティ 生細胞におけるタンパク質−タンパク質相互作用の測定方法
EP1194539A2 (de) * 1999-06-25 2002-04-10 Universität Zürich Hetero-assoziierende coiled-coil peptide und screenings-verfahren dafür

Also Published As

Publication number Publication date
EP1198586B1 (de) 2010-03-03
CA2377573A1 (en) 2001-01-04
ATE459724T1 (de) 2010-03-15
EP1198586A4 (de) 2004-03-24
EP1198586A1 (de) 2002-04-24
WO2001000866A1 (en) 2001-01-04
AU6054900A (en) 2001-01-31
JP2003503067A (ja) 2003-01-28

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee