WO2002090584A3 - Procédé de marquage et de fragmentation d'adn - Google Patents

Procédé de marquage et de fragmentation d'adn Download PDF

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Publication number
WO2002090584A3
WO2002090584A3 PCT/FR2002/001542 FR0201542W WO02090584A3 WO 2002090584 A3 WO2002090584 A3 WO 2002090584A3 FR 0201542 W FR0201542 W FR 0201542W WO 02090584 A3 WO02090584 A3 WO 02090584A3
Authority
WO
WIPO (PCT)
Prior art keywords
labelling
dna
fragmenting
reagent
fragmenting dna
Prior art date
Application number
PCT/FR2002/001542
Other languages
English (en)
Other versions
WO2002090584A2 (fr
Inventor
Cecile Bourget
Mitsuharu Kotera
Jean Lhomme
Emmanuelle Trevisiol
Ali Laayoun
Christelle Tora
Isabelle Sothier
Original Assignee
Bio Merieux
Univ Grenoble 1
Centre Nat Rech Scient
Cecile Bourget
Mitsuharu Kotera
Jean Lhomme
Emmanuelle Trevisiol
Ali Laayoun
Christelle Tora
Isabelle Sothier
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Merieux, Univ Grenoble 1, Centre Nat Rech Scient, Cecile Bourget, Mitsuharu Kotera, Jean Lhomme, Emmanuelle Trevisiol, Ali Laayoun, Christelle Tora, Isabelle Sothier filed Critical Bio Merieux
Priority to JP2002587643A priority Critical patent/JP4554159B2/ja
Priority to DE60214840T priority patent/DE60214840T2/de
Priority to EP02738211A priority patent/EP1383925B1/fr
Priority to CA2444926A priority patent/CA2444926C/fr
Priority to AU2002313026A priority patent/AU2002313026B2/en
Publication of WO2002090584A2 publication Critical patent/WO2002090584A2/fr
Publication of WO2002090584A3 publication Critical patent/WO2002090584A3/fr
Priority to HK04105111A priority patent/HK1062033A1/xx

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

La présente invention concerne un procédé de marquage et de fragmentation d'un acide désoxyribonucléique (ADN) simple ou double brin comprenant les étapes suivantes : fragmenter chimiquement l'ADN en créant au moins un site abasique sur ledit ADN, attacher un marqueur sur au moins un des fragments par l'intermédiaire d'un réactif de marquage, ledit réactif se couplant de manière covalente et majoritaire sur au moins un phosphate dudit fragment. L'invention trouve une application préférentielle dans le domaine du diagnostic.
PCT/FR2002/001542 2001-05-04 2002-05-03 Procédé de marquage et de fragmentation d'adn WO2002090584A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP2002587643A JP4554159B2 (ja) 2001-05-04 2002-05-03 Ｄｎａを標識化および断片化する方法
DE60214840T DE60214840T2 (de) 2001-05-04 2002-05-03 Verfahren zur markierung und fragmentierung von dns
EP02738211A EP1383925B1 (fr) 2001-05-04 2002-05-03 Procede de marquage et de fragmentation d'adn
CA2444926A CA2444926C (fr) 2001-05-04 2002-05-03 Procede de marquage et de fragmentation d'adn
AU2002313026A AU2002313026B2 (en) 2001-05-04 2002-05-03 Method for labelling and fragmenting DNA
HK04105111A HK1062033A1 (en) 2001-05-04 2004-07-13 Method for labelling and fragmenting dna

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR01/06039 2001-05-04
FR0106039A FR2824335A1 (fr) 2001-05-04 2001-05-04 Procede de marquage et de fragmentation d'adn

Publications (2)

Publication Number Publication Date
WO2002090584A2 WO2002090584A2 (fr) 2002-11-14
WO2002090584A3 true WO2002090584A3 (fr) 2003-09-25

Family

ID=8863020

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2002/001542 WO2002090584A2 (fr) 2001-05-04 2002-05-03 Procédé de marquage et de fragmentation d'adn

Country Status (11)

Country Link
EP (1) EP1383925B1 (fr)
JP (2) JP4554159B2 (fr)
CN (1) CN1325659C (fr)
AT (1) ATE340269T1 (fr)
AU (1) AU2002313026B2 (fr)
CA (1) CA2444926C (fr)
DE (1) DE60214840T2 (fr)
ES (1) ES2272723T3 (fr)
FR (1) FR2824335A1 (fr)
HK (1) HK1062033A1 (fr)
WO (1) WO2002090584A2 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8143001B2 (en) 2003-12-29 2012-03-27 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
US9206418B2 (en) 2011-10-19 2015-12-08 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004011665A2 (fr) * 2002-05-17 2004-02-05 Nugen Technologies, Inc. Procedes de fragmentation, d'etiquetage et d'immobilisation d'acides nucleiques
CN1882703B (zh) * 2003-10-29 2011-07-06 佰尔瑞溶液有限公司 通过断裂双链脱氧核糖核酸进行多元核酸分析
US20050191682A1 (en) * 2004-02-17 2005-09-01 Affymetrix, Inc. Methods for fragmenting DNA
FR2868071B1 (fr) * 2004-03-26 2006-06-09 Biomerieux Sa Reactifs de marquage, procedes de synthese de tels reactifs et procedes de detection de molecules biologiques
FR2873388B1 (fr) * 2004-07-23 2012-04-06 Biomerieux Sa Procede de marquage et de purification d'acides nucleiques d'interet presents dans un echantillon biologique a traiter dans un unique recipient reactionnel
FR2881437B1 (fr) 2005-01-31 2010-11-19 Biomerieux Sa Procede pour le diagnostic/pronostic d'un syndrome septique
FR2881363B1 (fr) 2005-02-02 2008-03-14 Commissariat Energie Atomique Dispositif d'analyses biologiques avec detecteur integre
FR2917090B1 (fr) 2007-06-11 2012-06-15 Biomerieux Sa Reactifs de marquage portant des fonctions diazo et nitro, procedes de synthese de tels reactifs et procedes de detection de molecules biologiques
FR2934595B1 (fr) 2008-07-29 2013-04-05 Biomerieux Sa Reactifs de marquage ayant un noyau pyridine portant une fonction diazomethyle, procedes de synthese de tels reactifs et procedes de detection de molecules biologiques
JP5916718B2 (ja) 2010-06-04 2016-05-11 ビオメリューＢｉｏｍｅｒｉｅｕｘ 結腸直腸癌の予後判定のための方法及びキット
US9110079B2 (en) 2010-09-29 2015-08-18 Biomerieux Method and kit for establishing an in vitro prognosis on a patient exhibiting SIRS
WO2012129758A1 (fr) 2011-03-25 2012-10-04 Biomerieux Procédé et kit pour déterminer in vitro la probabilité qu'un individu soit atteint d'un cancer colorectal
WO2013112923A1 (fr) 2012-01-26 2013-08-01 Nugen Technologies, Inc. Compositions et procédés pour l'enrichissement en séquence d'acide nucléique ciblée et la génération d'une banque à efficacité élevée
WO2013191775A2 (fr) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions et procédés pour la sélection négative de séquences d'acide nucléique indésirable
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US20140274738A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
CA2929596C (fr) 2013-11-13 2022-07-05 Nugen Technologies, Inc. Compositions et procedes pour l'identification d'une lecture de sequencage en double
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
WO2016022833A1 (fr) 2014-08-06 2016-02-11 Nugen Technologies, Inc. Mesures numériques à partir de séquençage ciblé
CN104862302B (zh) * 2015-05-05 2020-12-15 华南师范大学 一种dna片段化的方法及实现该方法的装置
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
CN113636931B (zh) * 2021-08-05 2024-02-13 康龙化成(宁波)科技发展有限公司 基因编码化合物库起始头片段化合物及其在基因编码化合物库合成上的应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990008838A1 (fr) * 1989-01-10 1990-08-09 Research Corporation Technologies, Inc. Marquage d'acides nucleiques a l'aide de marqueurs fluorescents
WO1999065926A1 (fr) * 1998-06-17 1999-12-23 Bio Merieux Procede de marquage d'un acide ribonucleique et fragments d'arn marques ainsi obtenus

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10179179A (ja) * 1996-11-08 1998-07-07 Kyowa Medex Co Ltd 特定遺伝子配列の定量方法及び定量試薬
FR2824323B1 (fr) * 2001-05-04 2008-04-25 Bio Merieux Reactif de marquage et procede de detection de molecules biologiques

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990008838A1 (fr) * 1989-01-10 1990-08-09 Research Corporation Technologies, Inc. Marquage d'acides nucleiques a l'aide de marqueurs fluorescents
WO1999065926A1 (fr) * 1998-06-17 1999-12-23 Bio Merieux Procede de marquage d'un acide ribonucleique et fragments d'arn marques ainsi obtenus

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
MAKRIGIORGOS G M ET AL: "FLUORESCENT LABELLING OF ABASIC SITES: A NOVEL METHODOLOGY TO DETECT CLOSELY-SPACED DAMAGE SITES IN DNA", INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, TAYLOR AND FRANCIS, LONDON, GB, vol. 74, no. 1, 1998, pages 99 - 109, XP002925462, ISSN: 0955-3002 *
SHIGA M ET AL: "FLUORESCENCE DETECTION OF DNA USING A NOVEL PEROXIDASE SUBSTRATE, 4-(4-HYDROXYPHENYLCARBAMOYL)BUTANOIC ACID", ANALYTICAL SCIENCES, JAPAN SOCIETY FOR ANALYTICAL CHEMISTRY, TOKYO,, JP, vol. 11, no. 4, August 1995 (1995-08-01), pages 591 - 595, XP008005960, ISSN: 0910-6340 *
SHIGA M ET AL: "SYNTHESIS OF A NOVEL BIOTIN DERIVATIVE THAT BEARS A DIAZO GROUP AS THE REACTIVE SITE", ANALYTICAL SCIENCES, JAPAN SOCIETY FOR ANALYTICAL CHEMISTRY, TOKYO,, JP, vol. 9, no. 4, August 1993 (1993-08-01), pages 553 - 556, XP008005959, ISSN: 0910-6340 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8143001B2 (en) 2003-12-29 2012-03-27 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
US9206418B2 (en) 2011-10-19 2015-12-08 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing

Also Published As

Publication number Publication date
WO2002090584A2 (fr) 2002-11-14
FR2824335A1 (fr) 2002-11-08
ATE340269T1 (de) 2006-10-15
CN1639356A (zh) 2005-07-13
DE60214840T2 (de) 2007-09-06
AU2002313026B2 (en) 2007-06-28
JP2004527258A (ja) 2004-09-09
CN1325659C (zh) 2007-07-11
CA2444926A1 (fr) 2002-11-14
JP2010075211A (ja) 2010-04-08
EP1383925A2 (fr) 2004-01-28
CA2444926C (fr) 2011-03-22
DE60214840D1 (de) 2006-11-02
HK1062033A1 (en) 2004-10-15
EP1383925B1 (fr) 2006-09-20
JP4554159B2 (ja) 2010-09-29
ES2272723T3 (es) 2007-05-01

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