HK1062033A1 - Method for labelling and fragmenting dna - Google Patents

Method for labelling and fragmenting dna

Info

Publication number
HK1062033A1
HK1062033A1 HK04105111A HK04105111A HK1062033A1 HK 1062033 A1 HK1062033 A1 HK 1062033A1 HK 04105111 A HK04105111 A HK 04105111A HK 04105111 A HK04105111 A HK 04105111A HK 1062033 A1 HK1062033 A1 HK 1062033A1
Authority
HK
Hong Kong
Prior art keywords
labelling
dna
fragmenting
reagent
fragmenting dna
Prior art date
Application number
HK04105111A
Inventor
Cecile Bourget
Mitsuharu Kotera
Jean Lhomme
Emmanuelle Trevisiol
Ali Laayoun
Christelle Tora
Isabelle Sothier
Original Assignee
Bio Merieux
Univ Grenoble 1
Centre Nat Rech Scient
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Merieux, Univ Grenoble 1, Centre Nat Rech Scient filed Critical Bio Merieux
Publication of HK1062033A1 publication Critical patent/HK1062033A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

The invention concerns a method for labelling and fragmenting a single-stranded or double-stranded deoxyribonucleic acid (DNA) comprising the following steps: chemically fragmenting the DNA by generating an abasic site on said DNA, attaching a marker on at least one of the fragments by means of a labelling reagent, said reagent being covalently and majority bonded on at least one phosphate of said fragment. The invention is applicable in the field of diagnosis.
HK04105111A 2001-05-04 2004-07-13 Method for labelling and fragmenting dna HK1062033A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0106039A FR2824335A1 (en) 2001-05-04 2001-05-04 DNA MARKING AND FRAGMENTATION PROCESS
PCT/FR2002/001542 WO2002090584A2 (en) 2001-05-04 2002-05-03 Method for labelling and fragmenting dna

Publications (1)

Publication Number Publication Date
HK1062033A1 true HK1062033A1 (en) 2004-10-15

Family

ID=8863020

Family Applications (1)

Application Number Title Priority Date Filing Date
HK04105111A HK1062033A1 (en) 2001-05-04 2004-07-13 Method for labelling and fragmenting dna

Country Status (11)

Country Link
EP (1) EP1383925B1 (en)
JP (2) JP4554159B2 (en)
CN (1) CN1325659C (en)
AT (1) ATE340269T1 (en)
AU (1) AU2002313026B2 (en)
CA (1) CA2444926C (en)
DE (1) DE60214840T2 (en)
ES (1) ES2272723T3 (en)
FR (1) FR2824335A1 (en)
HK (1) HK1062033A1 (en)
WO (1) WO2002090584A2 (en)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1573056A4 (en) * 2002-05-17 2007-11-28 Nugen Technologies Inc Methods for fragmentation, labeling and immobilization of nucleic acids
WO2005045060A2 (en) * 2003-10-29 2005-05-19 Bioarray Solutions, Ltd. Multiplexed nucleic acid analysis by fragmentation of double-stranded dna
CA2552007A1 (en) 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
EP1564306B1 (en) * 2004-02-17 2013-08-07 Affymetrix, Inc. Methods for fragmenting and labeling DNA
FR2868071B1 (en) * 2004-03-26 2006-06-09 Biomerieux Sa MARKING REAGENTS, METHODS FOR SYNTHESIZING SUCH REAGENTS AND METHODS FOR DETECTING BIOLOGICAL MOLECULES
FR2873388B1 (en) * 2004-07-23 2012-04-06 Biomerieux Sa PROCESS FOR MARKING AND PURIFYING NUCLEIC ACIDS OF INTEREST PRESENT IN A BIOLOGICAL SAMPLE TO BE PROCESSED IN A SINGLE REACTION RECIPIENT
FR2881437B1 (en) 2005-01-31 2010-11-19 Biomerieux Sa METHOD FOR THE DIAGNOSIS / PROGNOSIS OF A SEPTIC SYNDROME
FR2881363B1 (en) 2005-02-02 2008-03-14 Commissariat Energie Atomique DEVICE FOR BIOLOGICAL ANALYZES WITH INTEGRATED DETECTOR
FR2917090B1 (en) * 2007-06-11 2012-06-15 Biomerieux Sa MARKING REAGENTS HAVING DIAZO AND NITRO FUNCTIONS, METHODS FOR SYNTHESIZING SUCH REAGENTS AND METHODS FOR DETECTING BIOLOGICAL MOLECULES
FR2934595B1 (en) * 2008-07-29 2013-04-05 Biomerieux Sa MARKING REAGENTS HAVING A PYRIDINE CORE HAVING DIAZOMETHYL FUNCTION, METHODS FOR SYNTHESIZING SUCH REAGENTS AND METHODS FOR DETECTING BIOLOGICAL MOLECULES
EP2576815B1 (en) 2010-06-04 2018-02-14 Biomérieux Method for the prognosis of colorectal cancer
US9110079B2 (en) 2010-09-29 2015-08-18 Biomerieux Method and kit for establishing an in vitro prognosis on a patient exhibiting SIRS
WO2012129758A1 (en) 2011-03-25 2012-10-04 Biomerieux Method and kit for determining in vitro probability for individual to suffer from colorectal cancer
GB2497838A (en) 2011-10-19 2013-06-26 Nugen Technologies Inc Compositions and methods for directional nucleic acid amplification and sequencing
CN105861487B (en) 2012-01-26 2020-05-05 纽亘技术公司 Compositions and methods for targeted nucleic acid sequence enrichment and efficient library generation
CN104619894B (en) 2012-06-18 2017-06-06 纽亘技术公司 For the composition and method of the Solid phase of unexpected nucleotide sequence
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
EP2971130A4 (en) 2013-03-15 2016-10-05 Nugen Technologies Inc Sequential sequencing
WO2015073711A1 (en) 2013-11-13 2015-05-21 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
CN107075581B (en) 2014-08-06 2022-03-18 纽亘技术公司 Digital measurement by targeted sequencing
CN104862302B (en) * 2015-05-05 2020-12-15 华南师范大学 DNA fragmentation method and device for implementing same
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
CN113636931B (en) * 2021-08-05 2024-02-13 康龙化成(宁波)科技发展有限公司 Gene coding compound library initial fragment compound and application thereof in synthesis of gene coding compound library

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2007431A1 (en) * 1989-01-10 1990-07-10 Larry W. Mclauglin Labeling of nucleic acids with fluorescent markers
JPH10179179A (en) * 1996-11-08 1998-07-07 Kyowa Medex Co Ltd Determination of specific gene sequence and reagent for determination
FR2780059B1 (en) * 1998-06-17 2002-10-11 Bio Merieux METHOD FOR LABELING A RIBONUCLEIC ACID AND BRANDED RNA FRAGMENTS THUS OBTAINED
FR2824323B1 (en) * 2001-05-04 2008-04-25 Bio Merieux MARKING REAGENT AND METHOD FOR DETECTING BIOLOGICAL MOLECULES

Also Published As

Publication number Publication date
AU2002313026B2 (en) 2007-06-28
DE60214840D1 (en) 2006-11-02
CA2444926A1 (en) 2002-11-14
CN1639356A (en) 2005-07-13
CN1325659C (en) 2007-07-11
CA2444926C (en) 2011-03-22
JP2010075211A (en) 2010-04-08
EP1383925A2 (en) 2004-01-28
JP4554159B2 (en) 2010-09-29
JP2004527258A (en) 2004-09-09
ES2272723T3 (en) 2007-05-01
DE60214840T2 (en) 2007-09-06
ATE340269T1 (en) 2006-10-15
WO2002090584A3 (en) 2003-09-25
WO2002090584A2 (en) 2002-11-14
EP1383925B1 (en) 2006-09-20
FR2824335A1 (en) 2002-11-08

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Legal Events

Date Code Title Description
PC Patent ceased (i.e. patent has lapsed due to the failure to pay the renewal fee)

Effective date: 20160503