WO2002079765A3 - Optische struktur zur multi-photonen-anregung und deren verwendung - Google Patents

Optische struktur zur multi-photonen-anregung und deren verwendung Download PDF

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Publication number
WO2002079765A3
WO2002079765A3 PCT/EP2002/002958 EP0202958W WO02079765A3 WO 2002079765 A3 WO2002079765 A3 WO 2002079765A3 EP 0202958 W EP0202958 W EP 0202958W WO 02079765 A3 WO02079765 A3 WO 02079765A3
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Prior art keywords
layer
excitation
optical structure
photon excitation
photon
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PCT/EP2002/002958
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English (en)
French (fr)
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WO2002079765A2 (de
Inventor
Gert L Duveneck
Martin A Bopp
Michael Pawlak
Markus Ehrat
Gerd Marowsky
Original Assignee
Zeptosens Ag
Gert L Duveneck
Martin A Bopp
Michael Pawlak
Markus Ehrat
Gerd Marowsky
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Publication date
Application filed by Zeptosens Ag, Gert L Duveneck, Martin A Bopp, Michael Pawlak, Markus Ehrat, Gerd Marowsky filed Critical Zeptosens Ag
Priority to EP02727426A priority Critical patent/EP1373875A2/de
Priority to JP2002577546A priority patent/JP2004530125A/ja
Priority to US10/473,325 priority patent/US20040052489A1/en
Priority to AU2002257671A priority patent/AU2002257671A1/en
Publication of WO2002079765A2 publication Critical patent/WO2002079765A2/de
Publication of WO2002079765A3 publication Critical patent/WO2002079765A3/de

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/55—Specular reflectivity
    • G01N21/552—Attenuated total reflection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/7703—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator using reagent-clad optical fibres or optical waveguides
    • G01N21/774—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator using reagent-clad optical fibres or optical waveguides the reagent being on a grating or periodic structure
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/7703—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator using reagent-clad optical fibres or optical waveguides
    • G01N2021/7706—Reagent provision
    • G01N2021/7709—Distributed reagent, e.g. over length of guide
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7769—Measurement method of reaction-produced change in sensor
    • G01N2021/7786—Fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7793—Sensor comprising plural indicators
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/10—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings of the optical waveguide type
    • G02B6/12—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings of the optical waveguide type of the integrated circuit kind
    • G02B2006/12083—Constructional arrangements
    • G02B2006/12107—Grating

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  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Plasma & Fusion (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Die Erfindung betrifft eine variable Ausführungsform einer optischen Struktur, umfassend einen optischen Wellenleiter, mit einer bei mindestens einer Anregungswellenlänge transparenten, wellenleitenden Schicht (a), dadurch gekennzeichnet, dass die Intensität eines in die Schicht (a) eingekoppelten und in der Schicht (a) geführten Anregungslichts auf der Schicht (a) und in der Schicht (a) ausreichend hoch ist, um auf der Oberfläche der Schicht (a) oder in einem Abstand von weniger als 200 nm zur Schicht (a) befindliche, lumineszenzfähige und/oder photoreaktive Moleküle mittels Multi-Photonen-Anregung, vorzugsweise 2-Photonen-Anregung, anzuregen. Bevorzugt sind dabei solche Auführungsformen, welche eine linienhafte oder flächenhafte Multi-Photonen-Anregung, längs des in der Schicht (a) geführten Anregungslichts, ermöglichen. Die Erfindung betrifft auch verschiedene Ausführungsformen optischer Systeme und analytischer Systeme mit einer Anregungslichtquelle und einer erfindungsgemässen Ausführung einer optischer Struktur sowie darauf basierende Verfahren, insbesondere zur Lumineszenzanregung sowie zum Lumineszenznachweis eines oder mehrerer Analyten mittels Multi-Photonen-Anregung, sowie deren Verwendung.
PCT/EP2002/002958 2001-04-02 2002-03-18 Optische struktur zur multi-photonen-anregung und deren verwendung WO2002079765A2 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP02727426A EP1373875A2 (de) 2001-04-02 2002-03-18 Optische struktur zur multi-photonen-anregung und deren verwendung
JP2002577546A JP2004530125A (ja) 2001-04-02 2002-03-18 多光子励起のための光学構造及びその使用
US10/473,325 US20040052489A1 (en) 2001-04-02 2002-03-18 Optical structure for multi-photon excitation and the use thereof
AU2002257671A AU2002257671A1 (en) 2001-04-02 2002-03-18 Optical structure for multi-photon excitation and the use thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CH6172001 2001-04-02
CH0617/01 2001-04-02
CH0689/01 2001-04-12
CH6892001 2001-04-12

Publications (2)

Publication Number Publication Date
WO2002079765A2 WO2002079765A2 (de) 2002-10-10
WO2002079765A3 true WO2002079765A3 (de) 2003-01-30

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PCT/EP2002/002958 WO2002079765A2 (de) 2001-04-02 2002-03-18 Optische struktur zur multi-photonen-anregung und deren verwendung

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Country Link
US (1) US20040052489A1 (de)
EP (1) EP1373875A2 (de)
JP (1) JP2004530125A (de)
AU (1) AU2002257671A1 (de)
WO (1) WO2002079765A2 (de)

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Publication number Publication date
EP1373875A2 (de) 2004-01-02
JP2004530125A (ja) 2004-09-30
US20040052489A1 (en) 2004-03-18
WO2002079765A2 (de) 2002-10-10
AU2002257671A1 (en) 2002-10-15

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