WO2002071072A3 - Verfahren und vorrichtung zur aufarbeitung von proteinen in gelen - Google Patents
Verfahren und vorrichtung zur aufarbeitung von proteinen in gelen Download PDFInfo
- Publication number
- WO2002071072A3 WO2002071072A3 PCT/EP2002/002334 EP0202334W WO02071072A3 WO 2002071072 A3 WO2002071072 A3 WO 2002071072A3 EP 0202334 W EP0202334 W EP 0202334W WO 02071072 A3 WO02071072 A3 WO 02071072A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- microtitre plate
- reaction vessels
- plate
- peptides
- gel
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
- B01L3/50255—Multi-well filtration
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2550/00—Electrophoretic profiling, e.g. for proteome analysis
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Pathology (AREA)
- Urology & Nephrology (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Clinical Laboratory Science (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Electrochemistry (AREA)
- Food Science & Technology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Sampling And Sample Adjustment (AREA)
- Peptides Or Proteins (AREA)
Abstract
Die Erfindung betrifft ein Verfahren zur Aufarbeitung von Proteinen in Gelen, indem Gelfragmente mit Proteinen aus dem Gel isoliert, einem Proteaseverdau unterworfen und die entstandenen Spaltpeptide identifiziert werden, wobei die isolierten Protein-Gelfragmente in die entsprechende Anzahl Reaktionsgefäße einer ersten Mikrotiterplatte (Filterplatte) überführt werden, wobei die Böden der Reaktionsgefäße der Mikrotiterplatte als hydrophobe Filtermembran ausgestaltet sind, die Protein-Gelfragmente gewaschen und mit der Proteaselösung inkubiert werden, wobei nach jedem Schritt die jeweilige Lösung durch die Filtermembran abgesaugt wird. Anschließend wird die erste Mikrotiterplatte über einer zweiten Mikrotiterplatte (Auffangplatte) postiert, wobei die Reaktionsgefäße der zweiten Mikrotiterplatte konische Böden aufweisen und aus einem Material bestehen, das keine Peptide adsorbiert, und dann die an den Filtermembranen der ersten Mikrotiterplatte gebundenen Peptide mittels hydrophober Waschlösung unter Anlegen von Druck in die Reaktionsgefäße der zweiten Mikrotiterplatte eluiert und aufkonzentriert werden.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2002256641A AU2002256641A1 (en) | 2001-03-05 | 2002-03-04 | Method and device for processing proteins in gels |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE2001111853 DE10111853A1 (de) | 2001-03-05 | 2001-03-05 | Verfahren und Vorrichtung zur Aufarbeitung von Proteinen in Gelen |
DE10111853.8 | 2001-03-05 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2002071072A2 WO2002071072A2 (de) | 2002-09-12 |
WO2002071072A3 true WO2002071072A3 (de) | 2003-04-24 |
Family
ID=7677166
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2002/002334 WO2002071072A2 (de) | 2001-03-05 | 2002-03-04 | Verfahren und vorrichtung zur aufarbeitung von proteinen in gelen |
Country Status (3)
Country | Link |
---|---|
AU (1) | AU2002256641A1 (de) |
DE (1) | DE10111853A1 (de) |
WO (1) | WO2002071072A2 (de) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2867695B1 (fr) | 2004-03-18 | 2006-12-29 | Portmann Instr | Dispositif et procede de filtration et/ou de separation de molecules |
WO2006136297A1 (en) | 2005-06-18 | 2006-12-28 | Ge Healthcare Bio-Sciences Ab | Methods and systems for adding a reagent to an analyte in a gel |
AU2015264168B2 (en) * | 2014-05-21 | 2020-03-12 | Unchained Labs | Systems and methods for exchange of buffer solutions |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4017805A1 (de) * | 1989-08-22 | 1991-03-07 | Finnigan Mat Gmbh | Verfahren, praeparat und vorrichtung zur bereitstellung eines analytes fuer eine untersuchung |
US5595636A (en) * | 1994-03-10 | 1997-01-21 | Bruker-Franzen Analytik Gmbh | Method for mass spectrometric analysis of samples from electrophoresis plates |
WO1998024543A1 (en) * | 1996-12-02 | 1998-06-11 | Glaxo Group Limited | Method and apparatus for transferring and combining reagents |
WO1998035753A1 (de) * | 1997-02-17 | 1998-08-20 | GESELLSCHAFT FüR BIOTECHNOLOGISCHE FORSCHUNG MBH (GBF) | Vorrichtung für eine automatisierte chemische synthese |
CA2244947A1 (en) * | 1998-06-30 | 1999-12-30 | Universite De Geneve | Method of identifying polypeptides |
EP1151793A1 (de) * | 2000-04-15 | 2001-11-07 | Bruker Daltonik GmbH | Behandlung von Proteinen aus Gelen zur Analyse durch Verwendung von Massenspektrometrie |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19602464B4 (de) * | 1996-01-24 | 2006-05-04 | Rapp, Wolfgang, Dr. | Vorrichtung zur multiplen, gleichzeitigen und parallelen Synthese chemischer Verbindungen und zur diskreten Weiterbehandlung von Aliquoten |
DE19933078B4 (de) * | 1999-07-19 | 2004-07-08 | Repairgenics Gmbh | Verfahren und Kit zur Bestimmung von DNA-Doppel-/Einzelstrangbrüchen und Vorrichtung zum kontrollierten Filtrieren von insbesondere DNA-Moleküle und/oder andere Moleküle/Molekülkomplexe enthaltenden flüssigen Medien mit Well-Filterplatten |
-
2001
- 2001-03-05 DE DE2001111853 patent/DE10111853A1/de not_active Withdrawn
-
2002
- 2002-03-04 AU AU2002256641A patent/AU2002256641A1/en not_active Abandoned
- 2002-03-04 WO PCT/EP2002/002334 patent/WO2002071072A2/de active Application Filing
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4017805A1 (de) * | 1989-08-22 | 1991-03-07 | Finnigan Mat Gmbh | Verfahren, praeparat und vorrichtung zur bereitstellung eines analytes fuer eine untersuchung |
US5595636A (en) * | 1994-03-10 | 1997-01-21 | Bruker-Franzen Analytik Gmbh | Method for mass spectrometric analysis of samples from electrophoresis plates |
WO1998024543A1 (en) * | 1996-12-02 | 1998-06-11 | Glaxo Group Limited | Method and apparatus for transferring and combining reagents |
WO1998035753A1 (de) * | 1997-02-17 | 1998-08-20 | GESELLSCHAFT FüR BIOTECHNOLOGISCHE FORSCHUNG MBH (GBF) | Vorrichtung für eine automatisierte chemische synthese |
CA2244947A1 (en) * | 1998-06-30 | 1999-12-30 | Universite De Geneve | Method of identifying polypeptides |
EP1151793A1 (de) * | 2000-04-15 | 2001-11-07 | Bruker Daltonik GmbH | Behandlung von Proteinen aus Gelen zur Analyse durch Verwendung von Massenspektrometrie |
Also Published As
Publication number | Publication date |
---|---|
AU2002256641A1 (en) | 2002-09-19 |
WO2002071072A2 (de) | 2002-09-12 |
DE10111853A1 (de) | 2002-11-14 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2003066842A3 (de) | Verfahren zur herstellung von rekombinanten proteinen in mikroorganismen | |
ATE377756T1 (de) | Verfahren und gerät für die identifikation und zuordnung von biomolekülen | |
WO2005121163A3 (en) | Isolation of plasma or serum proteins | |
WO2002053582A3 (en) | Process for producing peptides by using in vitrotranscription/translation system | |
WO2001000814A3 (en) | Hetero-associating coiled-coil peptides and screenign method therefor | |
WO2002014860A8 (en) | Functional protein arrays | |
DE60011390D1 (de) | Verfahren und vorrichtung zur identifikation oder karakterisierung von polypeptiden | |
GB0100513D0 (en) | Process | |
DK0832097T3 (da) | System og fremgangsmåde til modifikation og rensning af peptider | |
WO2002083710A3 (en) | Removal of bacterial endotoxin in a protein solution by immobilized metal affinity chromatography | |
WO2000008057A3 (en) | Molecules designated b7l-1 | |
WO2003004628A3 (de) | Verfahren zur aufreinigung eines enzyms und hiernach hergestelltes, aufgereinigtes enzym sowie verwendung des enzyms | |
EP1624065A3 (de) | Agarase und Gen dafür | |
AU2002238449A1 (en) | Process for the production and purification of secreted proteins ans the production of arrays of proteins | |
IL112329A (en) | Sucrose isomerase, dna sequences coding therefor and processes for the preparation of non-cariogenic sugars utilizing the same | |
WO2007063129A3 (en) | Insolation of peptides , polypeptides and proteins | |
WO2002071072A3 (de) | Verfahren und vorrichtung zur aufarbeitung von proteinen in gelen | |
EP1681299A3 (de) | Verfahren zur Reinigung stark anionischer Proteine | |
WO2023154822A3 (en) | Methods of purifying a product | |
MX9806304A (es) | Procedimiento para la purificacion de proteasas similares a trombina a partir de venenos de serpiente. | |
DE60100025D1 (de) | Behandlung von Proteinen aus Gelen zur Analyse durch Verwendung von Massenspektrometrie | |
WO2004051247A3 (en) | Electrophoretic device comprising separation chamber, non-uniform electrode chamber, and a porous membrane between them | |
WO2000068416A3 (de) | Methoden zur auffindung von proteasen, die spezifisch membrangebundene substrate spalten | |
CA2190802A1 (en) | A method of preparing and recovering proteins | |
WO2002090500A3 (en) | Novel human proteins, polynucleotides encoding them and methods of using the same |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A2 Designated state(s): AU CA US |
|
AL | Designated countries for regional patents |
Kind code of ref document: A2 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
122 | Ep: pct application non-entry in european phase |