WO2002071072A3 - Verfahren und vorrichtung zur aufarbeitung von proteinen in gelen - Google Patents

Verfahren und vorrichtung zur aufarbeitung von proteinen in gelen Download PDF

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Publication number
WO2002071072A3
WO2002071072A3 PCT/EP2002/002334 EP0202334W WO02071072A3 WO 2002071072 A3 WO2002071072 A3 WO 2002071072A3 EP 0202334 W EP0202334 W EP 0202334W WO 02071072 A3 WO02071072 A3 WO 02071072A3
Authority
WO
WIPO (PCT)
Prior art keywords
microtitre plate
reaction vessels
plate
peptides
gel
Prior art date
Application number
PCT/EP2002/002334
Other languages
English (en)
French (fr)
Other versions
WO2002071072A2 (de
Inventor
Andreas Koepke
Sascha Thies
Original Assignee
Wita Proteomics Ag
Andreas Koepke
Sascha Thies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wita Proteomics Ag, Andreas Koepke, Sascha Thies filed Critical Wita Proteomics Ag
Priority to AU2002256641A priority Critical patent/AU2002256641A1/en
Publication of WO2002071072A2 publication Critical patent/WO2002071072A2/de
Publication of WO2002071072A3 publication Critical patent/WO2002071072A3/de

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2550/00—Electrophoretic profiling, e.g. for proteome analysis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Clinical Laboratory Science (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Electrochemistry (AREA)
  • Food Science & Technology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zur Aufarbeitung von Proteinen in Gelen, indem Gelfragmente mit Proteinen aus dem Gel isoliert, einem Proteaseverdau unterworfen und die entstandenen Spaltpeptide identifiziert werden, wobei die isolierten Protein-Gelfragmente in die entsprechende Anzahl Reaktionsgefäße einer ersten Mikrotiterplatte (Filterplatte) überführt werden, wobei die Böden der Reaktionsgefäße der Mikrotiterplatte als hydrophobe Filtermembran ausgestaltet sind, die Protein-Gelfragmente gewaschen und mit der Proteaselösung inkubiert werden, wobei nach jedem Schritt die jeweilige Lösung durch die Filtermembran abgesaugt wird. Anschließend wird die erste Mikrotiterplatte über einer zweiten Mikrotiterplatte (Auffangplatte) postiert, wobei die Reaktionsgefäße der zweiten Mikrotiterplatte konische Böden aufweisen und aus einem Material bestehen, das keine Peptide adsorbiert, und dann die an den Filtermembranen der ersten Mikrotiterplatte gebundenen Peptide mittels hydrophober Waschlösung unter Anlegen von Druck in die Reaktionsgefäße der zweiten Mikrotiterplatte eluiert und aufkonzentriert werden.
PCT/EP2002/002334 2001-03-05 2002-03-04 Verfahren und vorrichtung zur aufarbeitung von proteinen in gelen WO2002071072A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002256641A AU2002256641A1 (en) 2001-03-05 2002-03-04 Method and device for processing proteins in gels

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE2001111853 DE10111853A1 (de) 2001-03-05 2001-03-05 Verfahren und Vorrichtung zur Aufarbeitung von Proteinen in Gelen
DE10111853.8 2001-03-05

Publications (2)

Publication Number Publication Date
WO2002071072A2 WO2002071072A2 (de) 2002-09-12
WO2002071072A3 true WO2002071072A3 (de) 2003-04-24

Family

ID=7677166

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/002334 WO2002071072A2 (de) 2001-03-05 2002-03-04 Verfahren und vorrichtung zur aufarbeitung von proteinen in gelen

Country Status (3)

Country Link
AU (1) AU2002256641A1 (de)
DE (1) DE10111853A1 (de)
WO (1) WO2002071072A2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2867695B1 (fr) 2004-03-18 2006-12-29 Portmann Instr Dispositif et procede de filtration et/ou de separation de molecules
WO2006136297A1 (en) 2005-06-18 2006-12-28 Ge Healthcare Bio-Sciences Ab Methods and systems for adding a reagent to an analyte in a gel
AU2015264168B2 (en) * 2014-05-21 2020-03-12 Unchained Labs Systems and methods for exchange of buffer solutions

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4017805A1 (de) * 1989-08-22 1991-03-07 Finnigan Mat Gmbh Verfahren, praeparat und vorrichtung zur bereitstellung eines analytes fuer eine untersuchung
US5595636A (en) * 1994-03-10 1997-01-21 Bruker-Franzen Analytik Gmbh Method for mass spectrometric analysis of samples from electrophoresis plates
WO1998024543A1 (en) * 1996-12-02 1998-06-11 Glaxo Group Limited Method and apparatus for transferring and combining reagents
WO1998035753A1 (de) * 1997-02-17 1998-08-20 GESELLSCHAFT FüR BIOTECHNOLOGISCHE FORSCHUNG MBH (GBF) Vorrichtung für eine automatisierte chemische synthese
CA2244947A1 (en) * 1998-06-30 1999-12-30 Universite De Geneve Method of identifying polypeptides
EP1151793A1 (de) * 2000-04-15 2001-11-07 Bruker Daltonik GmbH Behandlung von Proteinen aus Gelen zur Analyse durch Verwendung von Massenspektrometrie

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19602464B4 (de) * 1996-01-24 2006-05-04 Rapp, Wolfgang, Dr. Vorrichtung zur multiplen, gleichzeitigen und parallelen Synthese chemischer Verbindungen und zur diskreten Weiterbehandlung von Aliquoten
DE19933078B4 (de) * 1999-07-19 2004-07-08 Repairgenics Gmbh Verfahren und Kit zur Bestimmung von DNA-Doppel-/Einzelstrangbrüchen und Vorrichtung zum kontrollierten Filtrieren von insbesondere DNA-Moleküle und/oder andere Moleküle/Molekülkomplexe enthaltenden flüssigen Medien mit Well-Filterplatten

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4017805A1 (de) * 1989-08-22 1991-03-07 Finnigan Mat Gmbh Verfahren, praeparat und vorrichtung zur bereitstellung eines analytes fuer eine untersuchung
US5595636A (en) * 1994-03-10 1997-01-21 Bruker-Franzen Analytik Gmbh Method for mass spectrometric analysis of samples from electrophoresis plates
WO1998024543A1 (en) * 1996-12-02 1998-06-11 Glaxo Group Limited Method and apparatus for transferring and combining reagents
WO1998035753A1 (de) * 1997-02-17 1998-08-20 GESELLSCHAFT FüR BIOTECHNOLOGISCHE FORSCHUNG MBH (GBF) Vorrichtung für eine automatisierte chemische synthese
CA2244947A1 (en) * 1998-06-30 1999-12-30 Universite De Geneve Method of identifying polypeptides
EP1151793A1 (de) * 2000-04-15 2001-11-07 Bruker Daltonik GmbH Behandlung von Proteinen aus Gelen zur Analyse durch Verwendung von Massenspektrometrie

Also Published As

Publication number Publication date
AU2002256641A1 (en) 2002-09-19
WO2002071072A2 (de) 2002-09-12
DE10111853A1 (de) 2002-11-14

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