WO2002050257A3 - Verfahren zur herstellung von polyfructanen - Google Patents

Verfahren zur herstellung von polyfructanen Download PDF

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Publication number
WO2002050257A3
WO2002050257A3 PCT/EP2001/013524 EP0113524W WO0250257A3 WO 2002050257 A3 WO2002050257 A3 WO 2002050257A3 EP 0113524 W EP0113524 W EP 0113524W WO 0250257 A3 WO0250257 A3 WO 0250257A3
Authority
WO
WIPO (PCT)
Prior art keywords
inulin
production
fructooligosaccharides
relates
produced according
Prior art date
Application number
PCT/EP2001/013524
Other languages
English (en)
French (fr)
Other versions
WO2002050257A2 (de
Inventor
Dirk Engels
Begli Alireza Haji
Markwart Kunz
Ralf Mattes
Mohammad Munir
Manfred Vogel
Original Assignee
Suedzucker Ag
Dirk Engels
Begli Alireza Haji
Markwart Kunz
Ralf Mattes
Mohammad Munir
Manfred Vogel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10106163A external-priority patent/DE10106163B4/de
Application filed by Suedzucker Ag, Dirk Engels, Begli Alireza Haji, Markwart Kunz, Ralf Mattes, Mohammad Munir, Manfred Vogel filed Critical Suedzucker Ag
Priority to EP01989498A priority Critical patent/EP1346032A2/de
Priority to JP2002552134A priority patent/JP2004524826A/ja
Priority to AU2002227939A priority patent/AU2002227939A1/en
Priority to CA002431994A priority patent/CA2431994A1/en
Priority to US10/450,896 priority patent/US20040086902A1/en
Priority to MXPA03005645A priority patent/MXPA03005645A/es
Publication of WO2002050257A2 publication Critical patent/WO2002050257A2/de
Publication of WO2002050257A3 publication Critical patent/WO2002050257A3/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/04—Anorexiants; Antiobesity agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)

Abstract

Die vorliegende Erfindung betrifft Nucleinsäuren, die modifizierte Polypeptide mit Fructosyltransferase-Aktivität codieren, diese Nucleinsäure enthaltende Vektoren zur Expression der modifizierten Fructosyltransferase in pro- oder eukaryontischen Zellen, diese Vektoren enthaltende Wirtszellen und/oder transgene Pflanzen, Verfahren zur Herstellung von hochmolekularen Polyfructanen mit vorweigend βbeta-1, 2-Bindungen und einem sehr niedrigen Verzwendung des erfindungsgemäss hergestellten Inulins oder unter Verwendung von Saccharose, sowie Verfahren zur Herstellung von Difructosedianhydriden unter Verwendung des erfindungsgemäss hergestellten Inulins oder unter Verwendung von Saccharose, sowie die Verwendung des erfindungsgemäss hergestellten Inulins zur Herstellung von INulinethern und INulinestern und die Verwendung von Fructooligosacchariden oder hydrierten Fructooligosacchariden und Difructosedianhydriden als Lebensmittelszusatz, insbesondere als diätisches Lebensmittel.
PCT/EP2001/013524 2000-12-21 2001-11-21 Verfahren zur herstellung von polyfructanen WO2002050257A2 (de)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP01989498A EP1346032A2 (de) 2000-12-21 2001-11-21 Verfahren zur herstellung von kohlenhydraten
JP2002552134A JP2004524826A (ja) 2000-12-21 2001-11-21 ポリフルクタンの製造方法
AU2002227939A AU2002227939A1 (en) 2000-12-21 2001-11-21 Method for the production of polyfructans
CA002431994A CA2431994A1 (en) 2000-12-21 2001-11-21 Method for the production of polyfructans
US10/450,896 US20040086902A1 (en) 2000-12-21 2001-11-21 Method for the production of polyfructans
MXPA03005645A MXPA03005645A (es) 2000-12-21 2001-11-21 Procedimiento para la preparacion de carbohidratos.

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10063889 2000-12-21
DE10063889.9 2000-12-21
DE10106163.3 2001-02-10
DE10106163A DE10106163B4 (de) 2000-12-21 2001-02-10 Verfahren zur Herstellung von Kohlenhydraten

Publications (2)

Publication Number Publication Date
WO2002050257A2 WO2002050257A2 (de) 2002-06-27
WO2002050257A3 true WO2002050257A3 (de) 2003-01-30

Family

ID=26008020

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/013524 WO2002050257A2 (de) 2000-12-21 2001-11-21 Verfahren zur herstellung von polyfructanen

Country Status (7)

Country Link
US (1) US20040086902A1 (de)
EP (1) EP1346032A2 (de)
JP (1) JP2004524826A (de)
AU (1) AU2002227939A1 (de)
CA (1) CA2431994A1 (de)
MX (1) MXPA03005645A (de)
WO (1) WO2002050257A2 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006118481A1 (en) * 2005-05-04 2006-11-09 Ludan Arturo C Acesulfame-k inulin sweetener
JP5554958B2 (ja) * 2009-10-14 2014-07-23 青葉化成株式会社 野菜の軟化抑制剤、野菜の軟化抑制方法および加熱野菜
CN108642036B (zh) * 2018-05-15 2021-11-30 福建农林大学 一种镍掺杂的有序介孔氧化铝及固定化果糖基转移酶的制备方法
CN114085294B (zh) * 2021-11-18 2022-10-14 青岛农业大学 一种藏黄连多糖硒纳米粒结构表征及其活性研究方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994014970A1 (en) * 1992-12-28 1994-07-07 Stichting Scheikundig Onderzoek In Nederland Method for obtaining transgenic plants showing a modified fructan pattern
WO1997042331A1 (de) * 1996-05-03 1997-11-13 Südzucker Aktiengesellschaft Verfahren zur herstellung transgener, inulin erzeugender pflanzen
WO1999024593A1 (en) * 1997-11-06 1999-05-20 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Nucleic acid molecules which encode proteins having fructosyl transferase activity and methods for producing long-chain inulin
WO2000014246A1 (de) * 1998-09-02 2000-03-16 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Nucleinsäuremoleküle codierend enzyme, die fructosyltransferaseaktivität besitzen und deren verwendung

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01225492A (ja) * 1988-03-07 1989-09-08 Mitsubishi Kasei Corp ジフルクトース・ジアンヒドリド３の製造法
DE4341780A1 (de) * 1993-12-08 1995-06-14 Suedzucker Ag Hydrierte Fructooligosaccharide
NL1000064C1 (nl) * 1994-07-08 1996-01-08 Stichting Scheikundig Onderzoe Produktie van oligosacchariden in transgene planten.
ATE373094T1 (de) * 1995-01-06 2007-09-15 Plant Res Int Bv Für kohlenhydratpolymere-bildende enzyme- kodierende dna-sequenzen und verfahren zur herstellung transgener pflanzen

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994014970A1 (en) * 1992-12-28 1994-07-07 Stichting Scheikundig Onderzoek In Nederland Method for obtaining transgenic plants showing a modified fructan pattern
WO1997042331A1 (de) * 1996-05-03 1997-11-13 Südzucker Aktiengesellschaft Verfahren zur herstellung transgener, inulin erzeugender pflanzen
WO1999024593A1 (en) * 1997-11-06 1999-05-20 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Nucleic acid molecules which encode proteins having fructosyl transferase activity and methods for producing long-chain inulin
WO2000014246A1 (de) * 1998-09-02 2000-03-16 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Nucleinsäuremoleküle codierend enzyme, die fructosyltransferaseaktivität besitzen und deren verwendung

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
SATO S ET AL: "ISOLATION AND CHARACTERIZATION OF A FRUCTOSYLTRANSFERASE GENE FROM STREPTOCOCCUS MUTANTS GS-5", INFECTION AND IMMUNITY, vol. 52, no. 1, April 1986 (1986-04-01), pages 166 - 170, XP001036906, ISSN: 0019-9567 *
SHIROZA T ET AL: "SEQUENCE ANALYSIS OF THE STREPTOCOCCUS MUTANS FRUCTOSYLTRANSFERASE GENE AND FLANKING REGIONS", JOURNAL OF BACTERIOLOGY, vol. 170, no. 2, February 1988 (1988-02-01), pages 810 - 816, XP001002139, ISSN: 0021-9193 *

Also Published As

Publication number Publication date
MXPA03005645A (es) 2004-04-21
AU2002227939A1 (en) 2002-07-01
CA2431994A1 (en) 2002-06-27
JP2004524826A (ja) 2004-08-19
EP1346032A2 (de) 2003-09-24
US20040086902A1 (en) 2004-05-06
WO2002050257A2 (de) 2002-06-27

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