WO2002040126A3 - Procedes d'identification de nucleotides dans des positions definies dans des acides nucleiques cibles au moyen de la polarisation de fluorescence - Google Patents

Procedes d'identification de nucleotides dans des positions definies dans des acides nucleiques cibles au moyen de la polarisation de fluorescence Download PDF

Info

Publication number
WO2002040126A3
WO2002040126A3 PCT/US2001/030743 US0130743W WO0240126A3 WO 2002040126 A3 WO2002040126 A3 WO 2002040126A3 US 0130743 W US0130743 W US 0130743W WO 0240126 A3 WO0240126 A3 WO 0240126A3
Authority
WO
WIPO (PCT)
Prior art keywords
target nucleic
fluorescence polarization
methods
nucleic acids
defined positions
Prior art date
Application number
PCT/US2001/030743
Other languages
English (en)
Other versions
WO2002040126A2 (fr
Inventor
Ness Jeffrey Van
David J Galas
Lori K Garrison
Original Assignee
Keck Graduate Inst
Ness Jeffrey Van
David J Galas
Lori K Garrison
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to AU3922802A priority Critical patent/AU3922802A/xx
Application filed by Keck Graduate Inst, Ness Jeffrey Van, David J Galas, Lori K Garrison filed Critical Keck Graduate Inst
Priority to AU2002239228A priority patent/AU2002239228A1/en
Priority to US10/398,101 priority patent/US20040038256A1/en
Publication of WO2002040126A2 publication Critical patent/WO2002040126A2/fr
Publication of WO2002040126A3 publication Critical patent/WO2002040126A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

L'invention concerne un procédé d'identification d'un nucléotide dans une position définie dans l'acide nucléique cible au moyen d'endonucléases de restriction et de polarisation de fluorescence. L'invention concerne également des composés, des compositions, et des nécessaires associés à ce procédé.
PCT/US2001/030743 2000-10-02 2001-10-01 Procedes d'identification de nucleotides dans des positions definies dans des acides nucleiques cibles au moyen de la polarisation de fluorescence WO2002040126A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU3922802A AU3922802A (en) 2000-10-02 2001-08-31 Methods for identifying nucleotides at defined positions in target nucleic acidsusing fluorescence polarization
AU2002239228A AU2002239228A1 (en) 2000-10-02 2001-10-01 Methods for identifying nucleotides at defined positions in target nucleic acids using fluorescence polarization
US10/398,101 US20040038256A1 (en) 2001-10-01 2001-10-01 Methods for identifying nucleotides at defined positions in target nucleic acids using fluorescence polarization

Applications Claiming Priority (22)

Application Number Priority Date Filing Date Title
US23740900P 2000-10-02 2000-10-02
US60/237,409 2000-10-02
US24717300P 2000-11-10 2000-11-10
US24716600P 2000-11-10 2000-11-10
US24717200P 2000-11-10 2000-11-10
US24716700P 2000-11-10 2000-11-10
US24727500P 2000-11-10 2000-11-10
US60/247,172 2000-11-10
US60/247,173 2000-11-10
US60/247,166 2000-11-10
US60/247,275 2000-11-10
US60/247,167 2000-11-10
US26397101P 2001-01-24 2001-01-24
US60/263,971 2001-01-24
US26924401P 2001-02-15 2001-02-15
US60/269,244 2001-02-15
US30035001P 2001-06-21 2001-06-21
US30031901P 2001-06-21 2001-06-21
US60/300,350 2001-06-21
US60/300,319 2001-06-21
US30139401P 2001-06-27 2001-06-27
US60/301,394 2001-06-27

Publications (2)

Publication Number Publication Date
WO2002040126A2 WO2002040126A2 (fr) 2002-05-23
WO2002040126A3 true WO2002040126A3 (fr) 2006-04-06

Family

ID=27582742

Family Applications (4)

Application Number Title Priority Date Filing Date
PCT/US2001/030828 WO2002028501A1 (fr) 2000-10-02 2001-10-01 Genotypage par chromatographie en phase liquide de fragments d'acides nucleiques courts
PCT/US2001/030743 WO2002040126A2 (fr) 2000-10-02 2001-10-01 Procedes d'identification de nucleotides dans des positions definies dans des acides nucleiques cibles au moyen de la polarisation de fluorescence
PCT/US2001/042432 WO2002029006A2 (fr) 2000-10-02 2001-10-01 Procédé pour le mesurage parallèle des variations génétiques
PCT/US2001/030742 WO2002046447A2 (fr) 2000-10-02 2001-10-01 Procede d'identification de nucleotides a des positions determines dans des acides nucleiques cibles

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2001/030828 WO2002028501A1 (fr) 2000-10-02 2001-10-01 Genotypage par chromatographie en phase liquide de fragments d'acides nucleiques courts

Family Applications After (2)

Application Number Title Priority Date Filing Date
PCT/US2001/042432 WO2002029006A2 (fr) 2000-10-02 2001-10-01 Procédé pour le mesurage parallèle des variations génétiques
PCT/US2001/030742 WO2002046447A2 (fr) 2000-10-02 2001-10-01 Procede d'identification de nucleotides a des positions determines dans des acides nucleiques cibles

Country Status (2)

Country Link
AU (4) AU3922802A (fr)
WO (4) WO2002028501A1 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA04000432A (es) * 2001-07-15 2004-10-27 Keck Graduate Inst Amplificacion de fragmentos de acido nucleico empleando agentes de mella.
US7033762B2 (en) * 2001-08-29 2006-04-25 Amersham Biosciences Corp Single nucleotide amplification and detection by polymerase
US7052839B2 (en) 2001-08-29 2006-05-30 Amersham Biosciences Corp Terminal-phosphate-labeled nucleotides and methods of use
US7223541B2 (en) 2001-08-29 2007-05-29 Ge Healthcare Bio-Sciences Corp. Terminal-phosphate-labeled nucleotides and methods of use
DK1421213T3 (da) 2001-08-29 2010-06-07 Ge Healthcare Bio Sciences Mærkede nukleosidpolyphosphater
US7560254B2 (en) 2001-08-29 2009-07-14 Ge Healthcare Bio-Sciences Corp. Allele specific primer extension
US7256019B2 (en) 2001-08-29 2007-08-14 Ge Healthcare Bio-Sciences Corp. Terminal phosphate blocked nucleoside polyphosphates
GB0228614D0 (en) * 2002-12-07 2003-01-15 Fu Guoliang Oligonucleotide guided analysis of gene expression
WO2004083432A1 (fr) 2003-03-21 2004-09-30 Academisch Ziekenhuis Leiden Modulation de la reconnaissance d'exons dans le pre-arnm par interference avec la structure d'arn secondaire
ES2639852T3 (es) 2007-10-26 2017-10-30 Academisch Ziekenhuis Leiden Medios y métodos para contrarrestar los trastornos musculares
USRE48468E1 (en) 2007-10-26 2021-03-16 Biomarin Technologies B.V. Means and methods for counteracting muscle disorders
EP2119783A1 (fr) 2008-05-14 2009-11-18 Prosensa Technologies B.V. Procédé pour l'omission efficace de l'exon (44) dans la dystrophie musculaire de Duchenne et moyens connexes
JP2012524540A (ja) 2009-04-24 2012-10-18 プロセンサ テクノロジーズ ビー．ブイ． Ｄｍｄを処置するためのイノシンを含むオリゴヌクレオチド
CN112251436A (zh) 2012-01-27 2021-01-22 比奥马林技术公司 治疗杜兴型肌营养不良症和贝克型肌营养不良症的具有改善特性的rna调节性寡核苷酸

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000011220A1 (fr) * 1998-08-21 2000-03-02 Washington University Polarisation de fluorescence utilisee dans l'analyse d'acides nucleiques

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6040166A (en) * 1985-03-28 2000-03-21 Roche Molecular Systems, Inc. Kits for amplifying and detecting nucleic acid sequences, including a probe
DE69333650T2 (de) * 1992-02-19 2006-01-12 The Public Health Research Institute Of The City Of New York, Inc. Neue anordnungn von oligonukleotiden und ihr nutzen zum sortieren, isolieren, sequenzieren und manipulieren von nukleinsäuren
JPH09503641A (ja) * 1992-05-22 1997-04-15 アメリカ合衆国 人体の多剤耐性遺伝子の発現およびそれら遺伝子によって形質導入された細胞の選択
US5284758A (en) * 1992-08-14 1994-02-08 Merck & Co., Inc. Process for forming cholesterol lowering compound using pseudodiplodia sp.
JPH078289A (ja) * 1993-06-29 1995-01-13 Osaka Gas Co Ltd リボソームを用いたペプチドおよびタンパク質の合成法
US6110709A (en) * 1994-03-18 2000-08-29 The General Hospital Corporation Cleaved amplified modified polymorphic sequence detection methods
US6027898A (en) * 1997-08-18 2000-02-22 Transgenomic, Inc. Chromatographic method for mutation detection using mutation site specifically acting enzymes and chemicals

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000011220A1 (fr) * 1998-08-21 2000-03-02 Washington University Polarisation de fluorescence utilisee dans l'analyse d'acides nucleiques

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CHEN ET AL.: "Fluorescence polarization in homogeneous nucleic acid analysis", GENOME RESEARCH, vol. 9, 1999, pages 492 - 498, XP000851703 *

Also Published As

Publication number Publication date
WO2002040126A2 (fr) 2002-05-23
AU2002239228A8 (en) 2006-11-02
WO2002029006A3 (fr) 2002-08-29
WO2002046447A3 (fr) 2003-12-24
WO2002028501A1 (fr) 2002-04-11
AU3922802A (en) 2002-05-27
WO2002046447A2 (fr) 2002-06-13
AU2001296491A1 (en) 2002-04-15
WO2002029006A2 (fr) 2002-04-11
AU1183902A (en) 2002-04-15
AU4146002A (en) 2002-06-18

Similar Documents

Publication Publication Date Title
AU2001276843A1 (en) Nucleic acid enzyme biosensor for ions
WO2002040126A3 (fr) Procedes d'identification de nucleotides dans des positions definies dans des acides nucleiques cibles au moyen de la polarisation de fluorescence
AU2002347981A1 (en) Universal nucleotides for nucleic acid analysis
AU2002225888A1 (en) Double transposition methods for manipulating nucleic acids
WO2002052031A3 (fr) Amplification d'acide nucleique
WO2003008622A3 (fr) Amplification exponentielle d'acides nucleiques au moyen d'endonucleases de coupure
AU2002314693A1 (en) Nucleic acid amplification method
AU2002322518A1 (en) Methods for preparing nucleic acid samples
WO2005087951A3 (fr) Reactifs, procedes et kits utilise dans la desactivation d'acides nucleiques
AU2002334672A1 (en) A clone-array pooled shotgun strategy for nucleic acid sequencing
AU2001287396A1 (en) Nucleic acid molecules, methods and kits for selecting a nucleic acid having a desired feature
WO2003078450A3 (fr) Liants du petit sillon a charge negative
AU2002217216A1 (en) Method for magnetising chemical or biological markers
AU2001285219A1 (en) Methods and compositions for identifying nucleic acid molecules using nucleolytic activities and hybridization
WO2000040590A3 (fr) Compose fonctionnalise, polynucleotide eventuellement marque et procede de detection d'un acide nucleique cible
AU2002346498A1 (en) Thermus brockianus nucleic acid polymerases
AU2002352902A1 (en) Thermus thermophilus nucleic acid polymerases
AU2002346517A1 (en) Thermus oshimai nucleic acid polymerases
AU5599101A (en) Methods for detecting nucleic acid molecules having particular nucleotide sequences
AU2038500A (en) Method for the detection of specific nucleic acid sequences by polymerase nucleotide incorporation
EP1098003A3 (fr) Procédé pour l' identification et la détection spécifique des mycobactéries qui grandissent lentement en utilisant le gène de l'ADN gyrase
AU2001262152A1 (en) Method for extracting nucleic acids
AU2214799A (en) Nucleic acid markers for apospory-specific genomic region
WO2002063037A3 (fr) Procede d'identification d'acides nucleiques fonctionnels
AU2002239228A1 (en) Methods for identifying nucleotides at defined positions in target nucleic acids using fluorescence polarization

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PH PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 10398101

Country of ref document: US

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
NENP Non-entry into the national phase

Ref country code: JP