WO2002031481A3 - Vorrichtung und verfahren zur elektrisch beschleunigten immobilisierung und zur detektion von molekülen - Google Patents

Vorrichtung und verfahren zur elektrisch beschleunigten immobilisierung und zur detektion von molekülen Download PDF

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Publication number
WO2002031481A3
WO2002031481A3 PCT/EP2001/011411 EP0111411W WO0231481A3 WO 2002031481 A3 WO2002031481 A3 WO 2002031481A3 EP 0111411 W EP0111411 W EP 0111411W WO 0231481 A3 WO0231481 A3 WO 0231481A3
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WO
WIPO (PCT)
Prior art keywords
macromolecules
immobilisation
sensor
detection
molecules
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PCT/EP2001/011411
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English (en)
French (fr)
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WO2002031481A2 (de
Inventor
Claus Escher
Guenter Brommer
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Aventis Res & Tech Gmbh & Co
Claus Escher
Guenter Brommer
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Application filed by Aventis Res & Tech Gmbh & Co, Claus Escher, Guenter Brommer filed Critical Aventis Res & Tech Gmbh & Co
Priority to AU2001291886A priority Critical patent/AU2001291886A1/en
Publication of WO2002031481A2 publication Critical patent/WO2002031481A2/de
Publication of WO2002031481A3 publication Critical patent/WO2002031481A3/de

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/0061—The surface being organic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00617—Delimitation of the attachment areas by chemical means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00621—Delimitation of the attachment areas by physical means, e.g. trenches, raised areas
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/0063—Other, e.g. van der Waals forces, hydrogen bonding
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00632—Introduction of reactive groups to the surface
    • B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00639—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium
    • B01J2219/00644—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium the porous medium being present in discrete locations, e.g. gel pads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00653—Making arrays on substantially continuous surfaces the compounds being bound to electrodes embedded in or on the solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
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  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

Beschrieben wird eine Vorrichtung zur elektronischen Immobilisierung und Detektion von Makromolekülen. Diese weist einen Träger (1) mit mehreren darauf angeordneten Sensorpositionen auf, auf denen Makromoleküle immobilisiert werden können, wobei jede Sensorposition aus mindestens einer Mobilisierungselektrode (3) und einer Sensorelektorde (4) besteht, die unabhängig voneinander mit elektrischen Potentialen belegt werden können, und wobei die Mobilisierungselektroden wenigstens einer Sensorposition mit einer Permeationsschicht (5) überzogen sind, die undurchlässig für die zu detektierenden Makromoleküle, aber durchlässig für Wasser und kleine Ionen ist, und wobei die Sensorelektroden entweder unbeschichtete Metallelektroden darstellen oder so ausgebildet sind, dass sie die zu detektierenden Makromoleküle spezifisch oder unspezifisch binden können. Mit der beschriebenen Vorrichtung lässt sich eine beschleunigte elektrische Immobilisierung und Hybridisierung sowie eine hochempfindliche elektrische Detektion von Molekülen durchführen.
PCT/EP2001/011411 2000-10-10 2001-10-02 Vorrichtung und verfahren zur elektrisch beschleunigten immobilisierung und zur detektion von molekülen WO2002031481A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001291886A AU2001291886A1 (en) 2000-10-10 2001-10-02 Device and method for electrically accelerated immobilisation and detection of molecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10049901.5 2000-10-10
DE2000149901 DE10049901C2 (de) 2000-10-10 2000-10-10 Vorrichtung und Verfahren zur elektrisch beschleunigten Immobilisierung und zur Detektion von Molekülen

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WO2002031481A2 WO2002031481A2 (de) 2002-04-18
WO2002031481A3 true WO2002031481A3 (de) 2002-08-29

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PCT/EP2001/011411 WO2002031481A2 (de) 2000-10-10 2001-10-02 Vorrichtung und verfahren zur elektrisch beschleunigten immobilisierung und zur detektion von molekülen

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AU (1) AU2001291886A1 (de)
DE (1) DE10049901C2 (de)
WO (1) WO2002031481A2 (de)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8877035B2 (en) 2005-07-20 2014-11-04 Bayer Healthcare Llc Gated amperometry methods
US9005527B2 (en) 2006-10-24 2015-04-14 Bayer Healthcare Llc Transient decay amperometry biosensors
US9110013B2 (en) 2005-09-30 2015-08-18 Bayer Healthcare Llc Gated voltammetry methods
US9410917B2 (en) 2004-02-06 2016-08-09 Ascensia Diabetes Care Holdings Ag Method of using a biosensor
CN106796192B (zh) * 2014-08-20 2019-10-29 ams国际有限公司 电容式传感器

Families Citing this family (13)

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US10539561B1 (en) 2001-08-30 2020-01-21 Customarray, Inc. Enzyme-amplified redox microarray detection process
GB0423686D0 (en) 2004-10-25 2004-11-24 Attana Ab Surface preparation method
US20060102471A1 (en) 2004-11-18 2006-05-18 Karl Maurer Electrode array device having an adsorbed porous reaction layer
US20070034513A1 (en) 2005-03-25 2007-02-15 Combimatrix Corporation Electrochemical deblocking solution for electrochemical oligomer synthesis on an electrode array
US9394167B2 (en) 2005-04-15 2016-07-19 Customarray, Inc. Neutralization and containment of redox species produced by circumferential electrodes
US20070065877A1 (en) 2005-09-19 2007-03-22 Combimatrix Corporation Microarray having a base cleavable succinate linker
WO2008020364A2 (en) * 2006-08-14 2008-02-21 Koninklijke Philips Electronics N. V. Biochemical sensor device
WO2009006445A2 (en) 2007-06-29 2009-01-08 Applied Biosystems Systems and methods for electronic detection with nanofets
WO2009076302A1 (en) 2007-12-10 2009-06-18 Bayer Healthcare Llc Control markers for auto-detection of control solution and methods of use
WO2009132667A1 (de) * 2008-04-30 2009-11-05 Micronas Gmbh Verfahren zum nachweisen und/oder bestimmen der konzentration eines liganden
US9927434B2 (en) 2010-01-20 2018-03-27 Customarray, Inc. Multiplex microarray of serially deposited biomolecules on a microarray
EP2565635B1 (de) 2011-09-02 2017-11-15 Sensirion AG Sensorchip und Verfahren zur Herstellung eines Sensorchips
EP3208610B1 (de) 2016-02-18 2021-05-12 ams AG Sensoranordnung und verfahren zur erzeugung von messsignalen

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9410917B2 (en) 2004-02-06 2016-08-09 Ascensia Diabetes Care Holdings Ag Method of using a biosensor
US8877035B2 (en) 2005-07-20 2014-11-04 Bayer Healthcare Llc Gated amperometry methods
US9110013B2 (en) 2005-09-30 2015-08-18 Bayer Healthcare Llc Gated voltammetry methods
US9005527B2 (en) 2006-10-24 2015-04-14 Bayer Healthcare Llc Transient decay amperometry biosensors
CN106796192B (zh) * 2014-08-20 2019-10-29 ams国际有限公司 电容式传感器

Also Published As

Publication number Publication date
DE10049901C2 (de) 2003-01-02
WO2002031481A2 (de) 2002-04-18
DE10049901A1 (de) 2002-05-02
AU2001291886A1 (en) 2002-04-22

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