WO2000075360A3 - Method and apparatus for enzyme detection - Google Patents

Method and apparatus for enzyme detection Download PDF

Info

Publication number
WO2000075360A3
WO2000075360A3 PCT/EP2000/004855 EP0004855W WO0075360A3 WO 2000075360 A3 WO2000075360 A3 WO 2000075360A3 EP 0004855 W EP0004855 W EP 0004855W WO 0075360 A3 WO0075360 A3 WO 0075360A3
Authority
WO
WIPO (PCT)
Prior art keywords
enzyme
enzyme detection
detecting
polymer
provides
Prior art date
Application number
PCT/EP2000/004855
Other languages
French (fr)
Other versions
WO2000075360A2 (en
Inventor
Steffi Krause
Claire Sumner
Original Assignee
Cambridge Life Sciences
Steffi Krause
Claire Sumner
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cambridge Life Sciences, Steffi Krause, Claire Sumner filed Critical Cambridge Life Sciences
Priority to EP00936818A priority Critical patent/EP1185688A2/en
Publication of WO2000075360A2 publication Critical patent/WO2000075360A2/en
Publication of WO2000075360A3 publication Critical patent/WO2000075360A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)

Abstract

The present invention provides a method for detecting the presence of an enzyme based on the enzyme-induced degradation of a polymer film. In a preferred embodiment, the invention provides a method for detecting the presence of an enzyme comprising contacting the sample to be analysed with a substrate, at least part of which is covered with a layer of a biodegradable polymer, said polymer being degraded by said enzyme to produce a signal; and measuring any signal produced.
PCT/EP2000/004855 1999-06-04 2000-05-27 Method and apparatus for enzyme detection WO2000075360A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP00936818A EP1185688A2 (en) 1999-06-04 2000-05-27 Method and apparatus for enzyme detection

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9913051.0 1999-06-04
GB9913051A GB2350677A (en) 1999-06-04 1999-06-04 Enzyme detection

Publications (2)

Publication Number Publication Date
WO2000075360A2 WO2000075360A2 (en) 2000-12-14
WO2000075360A3 true WO2000075360A3 (en) 2001-04-19

Family

ID=10854768

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/004855 WO2000075360A2 (en) 1999-06-04 2000-05-27 Method and apparatus for enzyme detection

Country Status (3)

Country Link
EP (1) EP1185688A2 (en)
GB (1) GB2350677A (en)
WO (1) WO2000075360A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0025084D0 (en) 2000-10-13 2000-11-29 Cambridge Meditech Improvements in detection
GB2435512A (en) 2006-02-23 2007-08-29 Mologic Ltd A binding assay and assay device
GB2435510A (en) 2006-02-23 2007-08-29 Mologic Ltd Enzyme detection product and methods
GB2435511A (en) 2006-02-23 2007-08-29 Mologic Ltd Protease detection
GB0620504D0 (en) 2006-10-16 2006-11-22 Queen Mary & Westfield College Method
TW200914822A (en) * 2007-05-03 2009-04-01 Koninkl Philips Electronics Nv Sensor system based on compound with solubility depending on analyte concentration
JP2010538292A (en) * 2007-09-06 2010-12-09 コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ Method and apparatus for chemical analysis of fluids
WO2011069997A2 (en) * 2009-12-09 2011-06-16 Iti Scotland Limited Detecting analytes
GB201012902D0 (en) 2010-07-30 2010-09-15 Queen Mary & Westfield College Sensor coating layer, device and method
GB2514825B (en) * 2013-06-06 2017-11-08 Bangor Univ Sensors
CN103555817B (en) * 2013-10-09 2015-08-19 上海工程技术大学 The dynamic degradation method of high-molecular biologic degradable material
CN107727861B (en) * 2017-08-22 2019-10-22 厦门依柯利斯医疗科技有限公司 A kind of pepsin assay kit and measuring method
BR112020006513A2 (en) * 2017-11-08 2020-09-29 Mölnlycke Health Care Ab biosensor and dressing system for associated injury

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5846744A (en) * 1993-05-29 1998-12-08 Cambridge Life Sciences Plc Sensors based on polymer transformation

Family Cites Families (7)

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US4444892A (en) * 1980-10-20 1984-04-24 Malmros Mark K Analytical device having semiconductive organic polymeric element associated with analyte-binding substance
US4916075A (en) * 1987-08-19 1990-04-10 Ohmicron Corporation Differential homogeneous immunosensor device
US5015843A (en) * 1990-02-15 1991-05-14 Polysense, Inc. Fiber optic chemical sensors based on polymer swelling
CA2043807A1 (en) * 1990-07-19 1992-01-20 Matthew K. Musho Conductive sensors and their use in diagnostic assays
GB2278447A (en) * 1993-05-29 1994-11-30 Cambridge Life Sciences Dielectric porosity change immunoassay
GB9415499D0 (en) * 1994-08-01 1994-09-21 Bartlett Philip N Electrodes and their use in analysis
GB9622304D0 (en) * 1996-10-26 1996-12-18 Univ Manchester Sensor

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5846744A (en) * 1993-05-29 1998-12-08 Cambridge Life Sciences Plc Sensors based on polymer transformation

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CHEN X. ET AL.: "Dynamic surface events measured by simultaneous probe microscopy and surface plasmon resonance", ANAL. CHEM., vol. 68, no. 1996, pages 1451 - 1455, XP002152742 *
DATABASE BIOSIS BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; XP002152743 *
HO W O ET AL: "ELECTROCHEMICAL SENSOR FOR MEASUREMENT OF UREA AND CREATININE IN SERUM BASED ON AC IMPEDANCE MEASUREMENT OF ENZYME-CATALYZED POLYMER TRANSFORMATION", ANALYTICAL CHEMISTRY,US,AMERICAN CHEMICAL SOCIETY. COLUMBUS, vol. 71, no. 10, 15 May 1999 (1999-05-15), pages 1940 - 1946, XP000834939, ISSN: 0003-2700 *
MCNEIL C.J. ET AL.: "Electrochemical sensors based on impendance measurement of enzyme-catalyzed polymer dissolution: theory and applications", ANAL. CHEM., vol. 67, 1995, pages 3928 - 3935, XP002151471 *
SAUM A.G.E ET AL.: "Use of substrate coated electrodes and AC impedance spectroscopy for the detection of enzyme activity", BIOSENSORS AND BIOELECTRONICS, vol. 13, 15 March 1998 (1998-03-15), pages 511 - 518, XP000984922, DOI: doi:10.1016/S0956-5663(97)00129-2 *
SUMNER C. ET AL.: "A transducer based on enzyme-induced degradation of thin polymer films monitored by surface plasmon resonance", ANAL. CHEM., vol. 72, 2000, pages 5225 - 5232, XP000961394 *

Also Published As

Publication number Publication date
WO2000075360A2 (en) 2000-12-14
GB9913051D0 (en) 1999-08-04
GB2350677A (en) 2000-12-06
EP1185688A2 (en) 2002-03-13

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