WO2001075151A3 - Verfahren zum erfassen von makromolekularen biopolymeren mittels einer elektrodenanordnung - Google Patents

Verfahren zum erfassen von makromolekularen biopolymeren mittels einer elektrodenanordnung Download PDF

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Publication number
WO2001075151A3
WO2001075151A3 PCT/DE2001/001244 DE0101244W WO0175151A3 WO 2001075151 A3 WO2001075151 A3 WO 2001075151A3 DE 0101244 W DE0101244 W DE 0101244W WO 0175151 A3 WO0175151 A3 WO 0175151A3
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WO
WIPO (PCT)
Prior art keywords
macromolecular biopolymers
electrode arrangement
molecules
electrodes
biopolymers
Prior art date
Application number
PCT/DE2001/001244
Other languages
English (en)
French (fr)
Other versions
WO2001075151A2 (de
Inventor
Franz Hofmann
Richard Johannes Luyken
Original Assignee
Infineon Technologies Ag
Franz Hofmann
Richard Johannes Luyken
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Infineon Technologies Ag, Franz Hofmann, Richard Johannes Luyken filed Critical Infineon Technologies Ag
Priority to US10/169,624 priority Critical patent/US20030226768A1/en
Priority to JP2001573025A priority patent/JP2003529773A/ja
Priority to EP01929290A priority patent/EP1272672A2/de
Publication of WO2001075151A2 publication Critical patent/WO2001075151A2/de
Publication of WO2001075151A3 publication Critical patent/WO2001075151A3/de

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3276—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a hybridisation with immobilised receptors

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Materials By The Use Of Fluid Adsorption Or Reactions (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

Die Elektroden sind mit Molekülen versehen, die makromolekulare Biopolymere binden können. Es wird eine erste elektrische Messung an den Elektroden durchgeführt. Ein Medium wird mit den Elektroden in Kontakt gebracht, derart, dass für den Fall, dass sich in dem Medium makromolekulare Biopolymere befinden, sich diese an auf den Elektroden aufgebrachte erste Moleküle oder zweite Moleküle spezifisch binden können. Nicht gebundene erste oder zweite Moleküle werden von der jeweiligen Elektrode entfernt und es wird eine zweite elektrische Messung durchgeführt. Abhängig von den Messungen werden die makromolekularen Biopolymere erfasst.
PCT/DE2001/001244 2000-03-30 2001-03-29 Verfahren zum erfassen von makromolekularen biopolymeren mittels einer elektrodenanordnung WO2001075151A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/169,624 US20030226768A1 (en) 2000-03-30 2001-03-29 Method for detecting macromolecular biopolymers by means of an electrode arrangement
JP2001573025A JP2003529773A (ja) 2000-03-30 2001-03-29 電極配置を用いた巨大分子生体高分子の検出方法
EP01929290A EP1272672A2 (de) 2000-03-30 2001-03-29 Verfahren zum erfassen von makromolekularen biopolymeren mittels einer elektrodenanordnung

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10015819.6 2000-03-30
DE10015819 2000-03-30

Publications (2)

Publication Number Publication Date
WO2001075151A2 WO2001075151A2 (de) 2001-10-11
WO2001075151A3 true WO2001075151A3 (de) 2002-04-18

Family

ID=7636970

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2001/001244 WO2001075151A2 (de) 2000-03-30 2001-03-29 Verfahren zum erfassen von makromolekularen biopolymeren mittels einer elektrodenanordnung

Country Status (4)

Country Link
US (1) US20030226768A1 (de)
EP (1) EP1272672A2 (de)
JP (1) JP2003529773A (de)
WO (1) WO2001075151A2 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7413859B2 (en) 2001-11-14 2008-08-19 Siemens Aktiengesellschaft Method and biosensors for detecting macromolecular biopolymers
DE10155892A1 (de) * 2001-11-14 2003-05-28 Infineon Technologies Ag Verfahren zum Erfassen von makromolekularen Biopolymeren mittels mindestens einer Einheit zum Immobilisieren von makromolekularen Biopolymeren und Biosensor zum Erfassen von makromolekularen Biopolymeren
DE10211358B4 (de) * 2002-03-14 2006-10-26 Siemens Ag Vertikal-Impedanz-Sensor-Anordnung und Verfahren zum Herstellen einer Vertikal-Impedanz-Sensor-Anordnung
US20050100938A1 (en) * 2002-03-14 2005-05-12 Infineon Technologies Ag Vertical impedance sensor arrangement and method for producing a vertical impedance sensor arrangement
DE10224567B4 (de) * 2002-06-03 2014-10-23 Boehringer Ingelheim Vetmedica Gmbh Sensor-Anordnung und Verfahren zum Betreiben einer Sensor-Anordnung
JP4101801B2 (ja) 2002-06-24 2008-06-18 シーメンス アクチエンゲゼルシャフト バイオセンサーアレイおよびその使用方法
DE10228125A1 (de) * 2002-06-24 2004-01-22 Infineon Technologies Ag Biosensor-Array und Verfahren zum Betreiben eines Biosensor-Arrays
DE10228124A1 (de) * 2002-06-24 2004-01-29 Infineon Technologies Ag Biosensor-Array und Verfahren zum Betreiben eines Biosensor-Arrays
DE10229210A1 (de) * 2002-06-28 2004-01-29 november Aktiengesellschaft Gesellschaft für Molekulare Medizin Vorrichtung zur Detektion eines Analyten
DE10259821B4 (de) * 2002-12-19 2006-03-09 Siemens Ag Bio-Chip
US7214528B1 (en) * 2002-12-31 2007-05-08 Oregon Health & Sciences University Device for direct electrical detection of molecules and molecule-molecule interactions
DE102004031370B4 (de) * 2004-06-29 2022-03-24 Siemens Aktiengesellschaft Vorrichtung und Verfahren zur Emulation einer Gegenelektrode in einem monolithisch integrierten elektrochemischen Analysesystem
DE102004031672A1 (de) * 2004-06-30 2006-01-19 Infineon Technologies Ag Planar-Sensor-Anordnung, Sensor-Array und Verfahren zum Herstellen einer Planar-Sensor-Anordnung
DE102004045210A1 (de) * 2004-09-17 2006-04-06 Infineon Technologies Ag Sensor-Anordnung und Verfahren zum Ermitteln eines Sensorereignisses
JP4779468B2 (ja) * 2005-07-01 2011-09-28 ソニー株式会社 相互作用検出部、バイオアッセイ用基板、及びそれらに係わる方法
AT503742B8 (de) * 2006-05-15 2011-08-15 Arc Austrian Res Centers Gmbh Elektronische biosensoranordnung
DE102007003161B4 (de) * 2007-01-22 2018-02-15 Intel Deutschland Gmbh Konfigurierbare Fernbedienung und entsprechende Verfahren zur Konfigurierung
US8834794B2 (en) * 2010-11-22 2014-09-16 Mehdi M Yazdanpanah Apparatus and methods for detection of tumor cells in blood

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995024641A1 (en) * 1994-03-08 1995-09-14 Andcare, Inc. Electrochemical immunoassay methods
US5658732A (en) * 1989-10-04 1997-08-19 E. I. Du Pont De Nemours And Company Assay method for biological target complexes on the surface of a biosensor
EP0859229A1 (de) * 1997-02-10 1998-08-19 Gist-Brocades B.V. Nachweis von Analyten unter Verwendung von Elektrochemie
EP0962773A1 (de) * 1998-06-03 1999-12-08 Mark Howard Jones Prüfverfahren, Vorrichtung und Markierungsmittel basierend auf Elektrochemie
WO2001061053A2 (en) * 2000-02-17 2001-08-23 Motorola, Inc. Protein and peptide sensors using electrical detection methods

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US5606662A (en) * 1995-03-24 1997-02-25 Advanced Micro Devices, Inc. Auto DRAM parity enable/disable mechanism

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5658732A (en) * 1989-10-04 1997-08-19 E. I. Du Pont De Nemours And Company Assay method for biological target complexes on the surface of a biosensor
WO1995024641A1 (en) * 1994-03-08 1995-09-14 Andcare, Inc. Electrochemical immunoassay methods
EP0859229A1 (de) * 1997-02-10 1998-08-19 Gist-Brocades B.V. Nachweis von Analyten unter Verwendung von Elektrochemie
EP0962773A1 (de) * 1998-06-03 1999-12-08 Mark Howard Jones Prüfverfahren, Vorrichtung und Markierungsmittel basierend auf Elektrochemie
WO2001061053A2 (en) * 2000-02-17 2001-08-23 Motorola, Inc. Protein and peptide sensors using electrical detection methods

Also Published As

Publication number Publication date
WO2001075151A2 (de) 2001-10-11
JP2003529773A (ja) 2003-10-07
EP1272672A2 (de) 2003-01-08
US20030226768A1 (en) 2003-12-11

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