WO2002031105A1 - Procede et instrument de collecte de liquide dans la cavite orale - Google Patents

Procede et instrument de collecte de liquide dans la cavite orale Download PDF

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Publication number
WO2002031105A1
WO2002031105A1 PCT/JP2000/007075 JP0007075W WO0231105A1 WO 2002031105 A1 WO2002031105 A1 WO 2002031105A1 JP 0007075 W JP0007075 W JP 0007075W WO 0231105 A1 WO0231105 A1 WO 0231105A1
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WO
WIPO (PCT)
Prior art keywords
oral
collection
fluid
collected
collecting
Prior art date
Application number
PCT/JP2000/007075
Other languages
English (en)
Japanese (ja)
Inventor
Toru Yokoyama
Naoki Shinozuka
Kenji Nakamura
Original Assignee
Sapporo Immuno Diagnostic Laboratory
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sapporo Immuno Diagnostic Laboratory filed Critical Sapporo Immuno Diagnostic Laboratory
Priority to PCT/JP2000/007075 priority Critical patent/WO2002031105A1/fr
Priority to AU2000276848A priority patent/AU2000276848A1/en
Publication of WO2002031105A1 publication Critical patent/WO2002031105A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/0045—Devices for taking samples of body liquids
    • A61B10/0051—Devices for taking samples of body liquids for taking saliva or sputum samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N2001/1056—Disposable (single-use) samplers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14—Suction devices, e.g. pumps; Ejector devices
    • G01N2001/1472—Devices not actuated by pressure difference
    • G01N2001/149—Capillaries; Sponges

Definitions

  • the present invention relates to the presence of oral secretions, such as salivary glands, submandibular glands, and sublingual glands, of saliva from each salivary gland, and exudates from the gingival sulcus, etc.
  • the present invention relates to a method for collecting an oral secretion fluid that is subjected to a treatment for sterilizing bacteria, and a simple instrument capable of performing sterilization, collection and collection.
  • a few microliters of whole blood obtained by puncturing a fingertip or the like is used as a test sample, and a test device consisting of a disposable biosensor chip and a sensor meter is used. After about 30 seconds, it is a system that can obtain the blood glucose level which is the test result (for example, WO00 / 07003).
  • the sample volume is as small as a few microliters and further volume reduction is being promoted, it is still an invasive or invasive measurement using a puncture needle, and the physical The pain and the danger of infectious diseases, especially, are inevitable No. For these reasons, there is an urgent need for noninvasive testing methods for glycemic patients who must perform frequent blood sampling for glycemic control.
  • sodium azide is mixed in the above-mentioned preservation solution to prevent propagation of oral bacteria present in the collected sample (saliva collection device “Omnisal (Saliva ⁇ Sampler) J)”.
  • saliva collection device “Omnisal (Saliva ⁇ Sampler) J) There is no D-glucose quantification, no sterilization operation is performed during collection, and no testing is performed on the spot and it is not used as a POCT, so the specimens stored in storage containers are transported to an inspection center, etc.
  • the remarkable progress of biosensor technology has led to the development of various POCT devices, and the need for simple instruments that can be collected immediately after sample collection.
  • the present invention solves the above-mentioned problem by disinfecting oral bacteria with a disinfectant in advance when collecting oral secretions for the purpose of quantifying D-dalcos in oral secretions. It has been shown that it suppresses the decomposition of D-dalcos by bacteria.
  • the oral secretory fluid is sterilized and collected by a hydrophilic absorbent containing a bactericide, and the hydrophilic absorbent is pressed to collect the absorbed oral secretory fluid. It is intended to provide a collection method and a simple device for collecting and collecting oral secretion fluid using the above method, thereby realizing rapid collection and collection of a sample. Description of the invention According to the present invention, when quantifying D-glucose contained in the oral secretion fluid, bacteria which are a nutrient source of D-glucose, which are various and abundant in the oral cavity, are preliminarily sterilized with a bactericide. And a new method for collecting oral secretions.
  • POCT it consists of only a hydrophilic absorber containing a bactericide, which has both the function of collecting oral secretory fluid at the same time as sterilization and the function of collecting oral secretory fluid after collection.
  • Another object of the present invention is to provide a simple device comprising a combination of an absorber and a water-resistant support.
  • Streptococcus mutans a cariogenic bacterium that is present in dental plaque, is capable of fermentatively decomposing various monosaccharides and producing several types of organic acids, including lactic acid. It is known (eg, Dental Microbiology, 5th Edition, Medical and Dental Medicine Publishing (1992)), and it is difficult to quantify D-glucose in saliva, an oral secretion fluid.
  • mouthwashes containing various disinfectants have been developed and sold as liquid toothpastes to prevent dental caries.
  • those containing cetylpyridinium chloride for example, W091 / 18585 (1991), JP-A-10-251131 (1998)) (for example, US Pat.No. 5,948,390 (1999)), and containing triclosan (For example, JP-A-11-310522, JP-A-11-322553) and the effect of triclosan and triclosan-monophosphate on the growth of oral bacteria (Journal of Antimicrobial Chemotherapy, Vol. 45, p. 447 (2000 )) And so on, and various bactericidal effects are being obtained.
  • the inclusion of a bactericide in the mouthwash is intended to prevent dental caries and periodontal disease, and a simple sampling method for the quantification of D-glucose in oral secretions has not yet been found. Therefore, in the present invention, as a method of collecting oral secretion fluid including a treatment of sterilizing oral bacteria for the purpose of quantifying D-glucose in oral secretion fluid, a method of collecting oral bacteria after disinfection using a bactericide is described.
  • the present invention provides a method for collecting oral secretions using one or both of the methods, which are performed simultaneously with or after sterilization.
  • the hydrophilic absorber in a structure in which only a hydrophilic absorber containing a bactericide or in combination with a water-resistant support, oral secretions are sterilized and collected by the hydrophilic absorber, and then the hydrophilic absorber is pressed. It also provides a method for collecting and collecting oral endocrine fluid for recovering absorbed oral secretion fluid, and a simple instrument for collection and recovery.
  • the hydrophilic absorber can be a collection and collection device with a separation function that can remove contaminants at the time of collection.
  • the collection layer By forming a multi-layer structure consisting of a filter layer and filtration layers of various pore sizes, the collection layer with a filtration function that allows the filtration of the oral secretory fluid components having a pore size equal to or less than the selected pore size when collected through the filtration layer.
  • a collection device is also provided.
  • the water-resistant support is made of a more flexible material in the form of a plate and a tube, so that when the oral secretory fluid is collected, the hydrophilic absorber placed above and inside the water-resistant support is used. The compression is easy due to its flexibility, and a device for collecting and collecting oral secretion fluid with a simple collection function is also provided.
  • the sensor chip and sensor meter which are POCT devices, are based on the reaction principle and fabrication technology that we have already invented (JP-A-2000-35413, WO00 / 04378, PCT / JP99 / 01392, PCT / JPOO / 05788, PCT / JPOO / 05789, PCT / JP00 / 05790), and the sensor chip consists of at least a working electrode and a counter electrode formed using a conductive material.
  • the layered structure on which the reaction reagent is immobilized is arranged on the upper surface of the electrode reaction part.
  • the sensor mechanism is composed of an electrochemical detection device and the like.
  • FIG. 1 shows the time course of the D-glucose abundance in human whole saliva in Example 1 of the present invention.
  • FIG. 2 shows the time course of the D-glucose abundance in human whole saliva with respect to various sterilized components in Example 2.
  • FIG. 3 shows the time course of the D-glucose abundance in human whole saliva by the bactericidal component (cetylpyridinium chloride (CPC)) in Example 3.
  • FIG. 4 shows a simple device for collecting and collecting oral secretion fluid (1) in Example 4
  • FIG. 5 shows a simple device for collecting and collecting oral secretion fluid in Example 5 (2)
  • FIG. This is a simple device (3) for collecting and collecting oral secretion fluid provided with a filtration function in Example 6.
  • 1 is a water-resistant support; 2 is a hydrophilic absorber; 3 is an oral secretion; 4 is an absorption layer; 5 is a filtration layer. Description of preferred embodiments
  • the bactericide used in the present invention can be used in the oral cavity without affecting the human body, sterilizes oral bacteria that degrade D-glucose, and does not inhibit D-glucose quantification. If so, there is no particular limitation.
  • CPC for quaternary ammonium salt-based germicides
  • ethanol for alcohol-based germicides
  • ozone water for peroxide-based germicides
  • triclosan and iodine-based germicides for phenol-based germicides.
  • a povidone or the like may be used. Therefore, it can also be used.
  • the hydrophilic absorber which collectively refers to the absorption layer, the adsorption layer, and the filtration layer, can be used in the oral cavity without affecting the human body, can contain a bactericide, and is compatible with the collected oral secretions. There is no particular limitation as long as there is no unspecific unintended adsorption or contamination of oral secretions, and it is a material that absorbs oral secretions and is hydrophilic.
  • fibers such as cotton, glass fiber, silica fiber, and cellulose fiber, and carboxymethyl cellulose, getyl aminoethyl cellulose, cellulose acetate, cellulose mixed ester, nylon, nitrocellulose, polyethersulfone, and polyester;
  • Polyethylene, polyvinyl chloride, polypropionate, polytetrafluoroethylene, polyvinylidene fluoride, polyvinylidene difluoride, polypropylene and the like can be used.
  • the absorption layer is not particularly limited as long as rapid absorption of oral secretions is realized.
  • the adsorbent layer should be made of a material that has positive or negative charge or a material that has ionic and covalent bonding capabilities, so long as it can realize a separation function that can remove contaminants by adsorption.
  • the filtration layer is not particularly limited as long as it realizes a filtration function capable of preparatively determining the molecular size of the recovered sample by selecting various pore sizes.
  • the water-resistant support can be used in the oral cavity without affecting the human body, and it can be applied with a bactericide, and non-specific unintended adsorption or oral secretion with the collected oral secretory fluid
  • a bactericide and non-specific unintended adsorption or oral secretion with the collected oral secretory fluid
  • tubes and films made of silicon, polyester, polyethylene, polyethylene terephthalate, polystyrene, polypropylene, etc. are inexpensive, and can be used because of their good adhesion to the hydrophilic absorber and good workability.
  • the substance for adhesion between the water-resistant support and the hydrophilic absorber can be used in the oral cavity without affecting the human body, and does not adsorb to the oral secretory fluid or contaminate the oral secretory fluid. There is no particular limitation as long as the substance is suitable.
  • Example 1 Change of D-glucose abundance in human whole saliva with time
  • FIG. 1 shows the change over time in the D-glucose abundance in human whole saliva in this example.
  • FIG. 2 shows the change over time of the D-Dalcos abundance ratio in human whole saliva with respect to various sterilizing components in the present example.
  • a commercially available oral washing solution containing a bactericidal component, etc. was added to the same human whole saliva so as to have a volume of 1/20, and the time-dependent change based on the D-glucose concentration immediately after the addition was measured. I asked.
  • FIG. 3 shows the time course of the D-dalcos abundance in whole saliva of humans by the sterilizing component (CPC) in this example.
  • CPC manufactured by Wako Pure Chemical Industries, Ltd.
  • D-glucose was added at a concentration of 5 mg / dL. did. Subsequent changes over time were determined based on the D-glucose concentration immediately after the addition.
  • the black circles in the figure indicate the results containing CPC, and the white circles in the figure indicate the results in FIG. 1 containing no fungicide.
  • FIG. 4 shows an example of a simple device for collecting and collecting oral secretions in this example.
  • Hydrophilic substrate 1 made of polypropylene (manufactured by Oji Yuka Synthetic Paper Co., Ltd.) impregnated with a CPC solution and dried to absorb hydrophilicity made of cellulose (manufactured by Nippon Pall Co., Ltd.)
  • Body 2 was placed with an adhesive. By contacting the oral secretory fluid 3 with the hydrophilic absorber 2, good absorbability was exhibited, sterilized and absorbed, and the oral secretory fluid 3 was rapidly collected.
  • the hydrophilic absorber 2 was pressed by bending the water-resistant support 1, so that the sterilized and collected oral secretions 3 could be collected from the end surface of the water-resistant support 1.
  • the stick shape of this simple device makes it easy to place it on the target site.It is easy to place saliva from the parotid gland beside the upper molars, and sublingual and submandibular gland saliva below the tongue. Sterilization and collection of spores were carried out easily.
  • the viscous substance considered to be mucin was trapped by the hydrophilic absorber 2 and became serous.
  • some of the food residue is captured by the hydrophilic absorber 2. It was confirmed that they would be caught.
  • FIG. 5 shows an example of a simple device for collecting and collecting oral secretions in this example.
  • the hydrophilic absorber 2 by selecting a material having an adsorbing ability for the hydrophilic absorber 2, for example, various protein concentrations can be applied to the adsorbing layer made of nylon (manufactured by Nippon Pall Co., Ltd.) having an adsorbing ability for proteins by covalent bonding.
  • nylon manufactured by Nippon Pall Co., Ltd.
  • Example 6 Simple instrument for collecting and collecting oral secretion fluid provided with a filtering function (3)
  • FIG. 6 shows an example of a simple device for collecting and collecting oral secretion fluid having a filtering function in this example. Things.
  • a two-layered hydrophilic absorber consisting of an absorbent layer 4 made of cellulose (manufactured by Nippon Pall Co., Ltd.) and a filtration layer 5 made of polyether sulfone (manufactured by Nippon Pall Co., Ltd.) is placed on the water-resistant support 1. And the intraoral portion where the residue of the food used in Example 1 can be seen Lactic acid 3 was sterilized and collected from one end of the tube, and collected from the other end of the tube.
  • the specific shape of the simple device for collecting and collecting oral secretions is illustrated, but the shape and arrangement of other components are not limited.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Public Health (AREA)
  • Hematology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Medical Informatics (AREA)
  • Molecular Biology (AREA)
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  • Animal Behavior & Ethology (AREA)
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  • Engineering & Computer Science (AREA)
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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne un procédé de collecte de liquide dans la cavité orale en vue de quantifier sa teneur en D-glucose. Les bactéries contenues dans la cavité orale sont soit préalablement tuées avec un bactéricide, soit recueillies en même temps que le fluide pour les empêcher de digérer le D-glucose. Par ailleurs, l'invention concerne un instrument approprié pour recueillir et récupérer le liquide dans la cavité orale, celui-ci étant stérilisé puis recueilli avec un élément absorbant hydrophile contenant un bactéricide. L'élément absorbant est ensuite pressé pour récupérer le liquide absorbé. Ainsi, l'invention permet de recueillir et de récupérer rapidement des échantillons.
PCT/JP2000/007075 2000-10-12 2000-10-12 Procede et instrument de collecte de liquide dans la cavite orale WO2002031105A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PCT/JP2000/007075 WO2002031105A1 (fr) 2000-10-12 2000-10-12 Procede et instrument de collecte de liquide dans la cavite orale
AU2000276848A AU2000276848A1 (en) 2000-10-12 2000-10-12 Method and instrument for collecting fluid in the oral cavity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2000/007075 WO2002031105A1 (fr) 2000-10-12 2000-10-12 Procede et instrument de collecte de liquide dans la cavite orale

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WO2002031105A1 true WO2002031105A1 (fr) 2002-04-18

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PCT/JP2000/007075 WO2002031105A1 (fr) 2000-10-12 2000-10-12 Procede et instrument de collecte de liquide dans la cavite orale

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002086453A1 (fr) 2001-04-20 2002-10-31 Sapporo Immuno Diagnostic Laboratory Instrument servant a prelever et a recuperer une secretion liquide d'une cavite orale
JP2014521092A (ja) * 2011-07-15 2014-08-25 オラシュアテクノロジーズ， インコーポレイテッド サンプル収集キット

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0485616A1 (fr) * 1990-05-29 1992-05-20 Sunstar Kabushiki Kaisha Composition orale
US5260031A (en) * 1990-12-18 1993-11-09 Saliva Diagnostic Systems, Inc. Saliva sampling device with sample adequacy indicating system
WO1995027205A1 (fr) * 1994-03-30 1995-10-12 Epitope, Inc. Dispositif et procede de dosage de la salive
WO1996004850A1 (fr) * 1994-08-09 1996-02-22 Epitope, Inc. Prelevement oral pour analyse immunologique
JPH0972900A (ja) * 1995-09-05 1997-03-18 Brother Ind Ltd 生化学物質測定装置及びそれに適用する唾液収集方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0485616A1 (fr) * 1990-05-29 1992-05-20 Sunstar Kabushiki Kaisha Composition orale
US5260031A (en) * 1990-12-18 1993-11-09 Saliva Diagnostic Systems, Inc. Saliva sampling device with sample adequacy indicating system
WO1995027205A1 (fr) * 1994-03-30 1995-10-12 Epitope, Inc. Dispositif et procede de dosage de la salive
WO1996004850A1 (fr) * 1994-08-09 1996-02-22 Epitope, Inc. Prelevement oral pour analyse immunologique
JPH0972900A (ja) * 1995-09-05 1997-03-18 Brother Ind Ltd 生化学物質測定装置及びそれに適用する唾液収集方法

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002086453A1 (fr) 2001-04-20 2002-10-31 Sapporo Immuno Diagnostic Laboratory Instrument servant a prelever et a recuperer une secretion liquide d'une cavite orale
EP1380829A1 (fr) * 2001-04-20 2004-01-14 Sapporo Immuno Diagnostic Laboratory Instrument servant a prelever et a recuperer une secretion liquide d'une cavite orale
EP1380829A4 (fr) * 2001-04-20 2009-12-30 Sapporo Immuno Diagnostic Lab Instrument servant a prelever et a recuperer une secretion liquide d'une cavite orale
JP2014521092A (ja) * 2011-07-15 2014-08-25 オラシュアテクノロジーズ， インコーポレイテッド サンプル収集キット

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