WO2002018903A1 - Materiel simple de collecte/recuperation d'echantillon liquide - Google Patents

Materiel simple de collecte/recuperation d'echantillon liquide Download PDF

Info

Publication number
WO2002018903A1
WO2002018903A1 PCT/JP2000/005790 JP0005790W WO0218903A1 WO 2002018903 A1 WO2002018903 A1 WO 2002018903A1 JP 0005790 W JP0005790 W JP 0005790W WO 0218903 A1 WO0218903 A1 WO 0218903A1
Authority
WO
WIPO (PCT)
Prior art keywords
liquid sample
collecting
sample
hydrophilic
absorber
Prior art date
Application number
PCT/JP2000/005790
Other languages
English (en)
Japanese (ja)
Inventor
Toru Yokoyama
Naoki Shinozuka
Kenji Nakamura
Original Assignee
Sapporo Immuno Diagnostic Laboratory
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sapporo Immuno Diagnostic Laboratory filed Critical Sapporo Immuno Diagnostic Laboratory
Priority to AU2000267325A priority Critical patent/AU2000267325A1/en
Priority to PCT/JP2000/005790 priority patent/WO2002018903A1/fr
Publication of WO2002018903A1 publication Critical patent/WO2002018903A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/505—Containers for the purpose of retaining a material to be analysed, e.g. test tubes flexible containers not provided for above
    • B01L3/5055—Hinged, e.g. opposable surfaces
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/15—Devices for taking samples of blood
    • A61B5/150007—Details
    • A61B5/150015—Source of blood
    • A61B5/150022—Source of blood for capillary blood or interstitial fluid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/15—Devices for taking samples of blood
    • A61B5/150007—Details
    • A61B5/150343—Collection vessels for collecting blood samples from the skin surface, e.g. test tubes, cuvettes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/15—Devices for taking samples of blood
    • A61B5/150007—Details
    • A61B5/150358—Strips for collecting blood, e.g. absorbent

Definitions

  • the present invention collects and collects liquid samples, for example, urine, saliva, sputum, swabs of throat, samples derived from living organisms such as sweat, tears, and blood, food analysis samples, and environmental measurement samples. It relates to possible methods and simple appliances. Background of the Invention
  • urinalysis includes 10 items such as protein, glucose, occult blood, etc., using the urine test paper method as a general test, and other endocrine tests such as electrolytes and minerals as biochemical tests. I have.
  • Urine collection is easier and less painful to subjects and patients than blood collection.
  • Saliva collection is not only painless, but also easy and anytime, anywhere.
  • POCT point-of-care testing
  • a saliva collection device “Omnisal (Saliva-Sampler)] manufactured by Saliva Diagnostic Systems, Inc.
  • a saliva collection device r SalivetteJ manufactured by Sarstedt
  • saliva, a non-invasive sample and a liquid sample as described above.
  • Excellent operability has been achieved with dedicated instruments such as a sampling instrument and a storage tube containing a preservation solution.
  • the sample stored in the storage container is transported to an inspection center or the like, where the sample is collected and the test is performed for the first time. Considering the above steps and required time, there is a problem with the speed.
  • the present invention provides a structure in which at least one layer of a hydrophilic absorber is disposed on a water-resistant support, wherein a liquid sample is collected by the hydrophilic absorber, and then the hydrophilic sample is collected.
  • a method of collecting and collecting a liquid sample which comprises compressing a body and collecting the absorbed liquid sample, and a simple instrument for collecting and collecting a liquid sample using the method. It is intended to realize quick collection and recovery. Description of the invention
  • the present invention realizes a method for collecting and collecting a specimen for simple and rapid examination. Specifically, in a structure in which at least one layer of a hydrophilic absorber is disposed on a water-resistant support, a liquid sample is collected by the hydrophilic absorber, and then the hydrophilic absorber is pressed to absorb the liquid sample. A method for collecting and collecting a liquid sample, characterized by collecting the liquid sample. Further, the present invention also provides a simple instrument for collecting and collecting a liquid sample using the above method.
  • the present invention also provides a method of recovering the absorbed liquid sample by pressing the hydrophilic absorber through the perforation hole.
  • the hydrophilic absorber is also characterized in that it has a separation function capable of removing the contaminant at the time of recovery by selecting a material having a property of adsorbing the contaminant in the liquid sample.
  • the separation function is provided by selecting a material having a positive or negative charge or a material having an ionic binding ability and a covalent binding ability for the adsorption layer.
  • the hydrophilic absorber into a multilayer structure comprising at least an absorption layer and filtration layers of various pore sizes, at the time of collection through the permeation hole, the liquid sample having a pore size equal to or smaller than the pore size selected for the filtration layer is obtained.
  • Another major feature is that it has a filtration function that allows fractionation.
  • filtration when collecting bacteria in a liquid sample, filtration can be performed by setting the pore size to 0.22 m.
  • the pore size in order to obtain blood plasma or serum components by filtering blood cell components from whole blood, the pore size can be reduced to 10 x m or 5 m or less.
  • Various whole blood filtration methods have been reported for separating plasma or serum using a porous component (eg, WO96 / 03194 (1996)).
  • the present invention also provides a simple collection and recovery method having both separation and filtration functions, which combines the above-mentioned contaminant removal method and fractionation method.
  • a sample derived from a living body such as blood, urine, and saliva
  • a sample for food analysis a sample for environmental measurement
  • a sample that can be liquefied can be used.
  • a compression plate with a perforated hole at the time of collection to avoid adherence to the practitioner.
  • FIG. 1 is a top view and a side view of a simple device for collecting and collecting a liquid sample in Example 1 of the present invention
  • FIG. 2 is a schematic diagram of a method of collecting a liquid sample using a compression plate in Example 1
  • FIG. 3 is a schematic diagram of a method of collecting and collecting a liquid sample having a separation function in Example 2;
  • FIG. 4 is a schematic diagram of a method for collecting and collecting a liquid sample having a filtration function according to the third embodiment.
  • the water-resistant support and the compression plate used in the present invention are machines that do not adsorb to the collected liquid sample or contaminate the liquid sample, are water-resistant, have good workability and are not damaged during use.
  • plastic films such as polyester, polyethylene, polyethylene terephthalate (PET), polystyrene, and polypropylene, and thick paper treated with water resistance are inexpensive. From the viewpoint of good adhesion to a hydrophilic absorber and good workability, it was found that water-treated paper was preferred here.
  • water-resistant paper may ease the disposal conditions of the used simple instrument after collection and collection.
  • the hydrophilic absorber which collectively refers to the absorption layer, adsorption layer, filtration layer, and spreading layer, is free from unspecific unintended adsorption with the collected liquid sample and contamination of the liquid sample, and is a material that absorbs the liquid sample And there is no particular limitation as long as it is hydrophilic.
  • fibers such as glass fiber, silica fiber, and cell mouth fiber, and carboxymethyl cellulose, methylaminoethyl cellulose, cell mouth varnish acetate, cellulose mixed ester, nylon, nitrocellulose, polyether sulfone, polyester, and polyester Len, polyvinyl chloride, polycarboxylic acid, polytetrafluoroethylene, polyvinylidene fluoride, polyvinylidene difluoride, polypropylene and the like can be used.
  • the absorption layer is not particularly limited as long as quick absorption of the liquid sample is realized.
  • the adsorbent layer should be made of a material that has positive or negative charge or a material that has ionic and covalent bonding capabilities, so long as it can realize a separation function that can remove contaminants by adsorption. There is no limitation.
  • the filtration layer is not particularly limited as long as it realizes a filtration function capable of preparatively determining the molecular size of the recovered sample by selecting various pore sizes.
  • the spreading layer is not particularly limited as long as it has a function of sequentially spreading the sample after filtration when the filtering layer is provided and improving the filtering efficiency.
  • the seal is water-resistant and does not adsorb to the collected liquid sample or contaminate the liquid sample. Furthermore, when collecting through the perforated hole, it shields around the perforated hole to prevent contamination. There is no particular limitation as long as it can hold a water-resistant support.
  • the adhesion between the water-resistant support and the hydrophilic absorber is not particularly limited as long as it is a substance that does not adsorb to the liquid sample or contaminate the liquid sample.
  • Example 1 Preparation of simple instrument for collecting and collecting liquid sample
  • FIG. 1 is a top view and a side view showing an example of manufacturing a simple instrument for collecting and collecting a liquid sample in the present embodiment.
  • FIG. 2 is a schematic diagram showing a method of recovering a liquid sample using a compression plate in the present embodiment.
  • a hydrophilic absorber 2 made of cellulose made by Nippon Pall Co., Ltd.
  • a water-resistant support 1 made of thick paper made by Oji Yuka Kagaku Synthetic Paper Co., Ltd.
  • the liquid sample 3 is brought into contact with the hydrophilic absorber 2;
  • the liquid sample 3 is already collected by the compression plate 5 having the transmission hole 4 in the insulating support 1 having the transmission hole 4.
  • the compression plate 5 having the transmission hole 4 in the insulating support 1 having the transmission hole 4.
  • FIG. 3 is a schematic diagram of a collection method in which a separation function is added to a simple device for collecting and collecting a liquid sample in the present embodiment.
  • a two-layer hydrophilic absorber 2 composed of an absorbent layer 6 made of cellulose (manufactured by Nippon Pall Co., Ltd.) and an adsorption layer 7 made of nylon (manufactured by Nippon Pall Co., Ltd.) was disposed on the water-resistant support 1. . Further, the transmission hole 4 on the lower surface side in the sample supply direction was shielded by a seal 10 in which an adhesive was applied to one surface of the PET film.
  • the selected adsorption layer 7 has the ability to adsorb proteins by covalent bonds.
  • the proteins in the sample were separated by the two-layer structure of the absorption layer 6 and the adsorption layer 7, but the same separation effect can be obtained by using only one layer of the adsorption layer 7. At that time, after removing the excess sample attached to the portion other than the adsorption layer 7, the sample can be recovered from the end surface of the water-resistant support 1 without using the permeation hole 4.
  • Example 3 Addition of filtration function
  • FIG. 4 is a schematic view of a collection method in which a filtering function is added to a simple device for collecting and collecting a liquid sample in the present embodiment.
  • the hydrophilic absorber 2 having a layer structure was disposed on the water-resistant support 1, and the hydrophilic absorber 2 was covered with a seal 10 in which an adhesive was applied to one surface of a PET film.
  • the transmission hole 4 on the lower surface side in the sample supply direction was also shielded by the seal 10. Whole blood as an actual sample was spotted from the sample supply hole 11 of the seal on the upper surface.
  • the seal 10 on the upper surface was partially peeled off, and separated from the other two layers so as to enclose the absorbent layer 6 containing the blood cell component in whole blood. Thereafter, the seal 10 on the lower surface was peeled off, and the filtration layer 8 and the developing layer 9 were pressed by the compression plate 5, whereby the liquid component in the whole blood could be collected from the permeation hole 4.
  • the filtration function of separating blood cells was provided by setting the pore size of the filtration layer 8 to 5 m, which is smaller than the size of blood cells in whole blood.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medical Informatics (AREA)
  • Surgery (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Physics & Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Dermatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne un procédé de collecte/récupération facile et rapide d'un échantillon à examiner, ainsi qu'un matériel simple servant à recueillir/récupérer un échantillon à l'aide du procédé ci-dessus. L'invention concerne notamment un procédé de collecte/récupération d'un échantillon liquide, caractérisé en ce qu'il comprend les étapes suivantes consistant dans une structure comprenant au moins une couche d'un absorbant hydrophile placé sur un support résistant à l'eau, à faire recueillir un échantillon liquide par cet absorbant hydrophile, puis à presser l'absorbant afin de récupérer l'échantillon liquide absorbé. L'invention concerne enfin un matériel simple de collecte/récupération d'un échantillon liquide, au moyen du procédé ci-dessus.
PCT/JP2000/005790 2000-08-28 2000-08-28 Materiel simple de collecte/recuperation d'echantillon liquide WO2002018903A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2000267325A AU2000267325A1 (en) 2000-08-28 2000-08-28 Simple implement for collecting/recovering liquid sample
PCT/JP2000/005790 WO2002018903A1 (fr) 2000-08-28 2000-08-28 Materiel simple de collecte/recuperation d'echantillon liquide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2000/005790 WO2002018903A1 (fr) 2000-08-28 2000-08-28 Materiel simple de collecte/recuperation d'echantillon liquide

Publications (1)

Publication Number Publication Date
WO2002018903A1 true WO2002018903A1 (fr) 2002-03-07

Family

ID=11736397

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2000/005790 WO2002018903A1 (fr) 2000-08-28 2000-08-28 Materiel simple de collecte/recuperation d'echantillon liquide

Country Status (2)

Country Link
AU (1) AU2000267325A1 (fr)
WO (1) WO2002018903A1 (fr)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0574134A2 (fr) * 1992-05-12 1993-12-15 Lifescan, Inc. Dispositif de conduite de fluide avec moyen de transport
EP0586789A2 (fr) * 1992-04-11 1994-03-16 Roche Diagnostics GmbH Membranes poreuses asymétriques
EP0597268A1 (fr) * 1992-10-13 1994-05-18 Bayer Corporation Dispositif et procédé pour l'analyse du HDL cholestérol dans le sang entier
JPH07134123A (ja) * 1993-09-17 1995-05-23 Nissho Corp 診断用容器
WO1996003194A1 (fr) * 1994-07-28 1996-02-08 Pall Corporation Bande fibreuse pour traiter un fluide
JPH0972900A (ja) * 1995-09-05 1997-03-18 Brother Ind Ltd 生化学物質測定装置及びそれに適用する唾液収集方法
JPH1096725A (ja) * 1996-07-29 1998-04-14 Hisamitsu Pharmaceut Co Inc 検査用デバイス

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0586789A2 (fr) * 1992-04-11 1994-03-16 Roche Diagnostics GmbH Membranes poreuses asymétriques
EP0574134A2 (fr) * 1992-05-12 1993-12-15 Lifescan, Inc. Dispositif de conduite de fluide avec moyen de transport
EP0597268A1 (fr) * 1992-10-13 1994-05-18 Bayer Corporation Dispositif et procédé pour l'analyse du HDL cholestérol dans le sang entier
JPH07134123A (ja) * 1993-09-17 1995-05-23 Nissho Corp 診断用容器
WO1996003194A1 (fr) * 1994-07-28 1996-02-08 Pall Corporation Bande fibreuse pour traiter un fluide
JPH0972900A (ja) * 1995-09-05 1997-03-18 Brother Ind Ltd 生化学物質測定装置及びそれに適用する唾液収集方法
JPH1096725A (ja) * 1996-07-29 1998-04-14 Hisamitsu Pharmaceut Co Inc 検査用デバイス

Also Published As

Publication number Publication date
AU2000267325A1 (en) 2002-03-13

Similar Documents

Publication Publication Date Title
US6048735A (en) Sensor laminates and multi-sectioned fluid delivery devices for detecting by immunoassay target molecules in biological fluids
US20130158431A1 (en) Sample collection system and method for use thereof
CA2452948C (fr) Bande d'essai pour dosage a ecoulement lateral d'un echantillon renfermant des cellules entieres
JP2729503B2 (ja) 粒子分離法及び装置
JP4191051B2 (ja) 血漿又は血清分離具
US8252139B2 (en) Method of making a fluid separator collection card
AU2006226195A1 (en) Device for sampling oral fluid
US5753497A (en) Diagnostic assay providing blood separation
JP2003532076A (ja) ラテラルフロー唾液検査用装置および方法
JPH10132800A (ja) 体液分離シートと一体型の検体保護容器
JP4675027B2 (ja) 口腔内分泌液の採取兼回収器具
WO2002018903A1 (fr) Materiel simple de collecte/recuperation d'echantillon liquide
JPH0843294A (ja) 全血試料の分析方法および分析要素
AU2013202899A1 (en) Method of processing a fluid sample using a fluid separator collection card
WO2002031105A1 (fr) Procede et instrument de collecte de liquide dans la cavite orale
JP3475834B2 (ja) 検査方法および用具
JP2004144636A (ja) 血液検体キャリアー構成体
AU2017203286B2 (en) Method of processing a fluid sample using a fluid separator multi-layer device
WO1998001753A1 (fr) Unite de separation du sang
TW200422615A (en) Test strip
WO1998010283A1 (fr) Recipient de protection d'echantillon solidaire d'une feuille de separation de fluides corporels
JPH0412272A (ja) 高感度乾式分折片

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AU BR CA CN CU IN JP KR MX NO NZ PL RO RU SG US

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): AT BE CH DE DK ES FR GB GR IT NL PT SE

121 Ep: the epo has been informed by wipo that ep was designated in this application
122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP