WO2002010834A1 - Table a microscope - Google Patents

Table a microscope Download PDF

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Publication number
WO2002010834A1
WO2002010834A1 PCT/EP2001/008412 EP0108412W WO0210834A1 WO 2002010834 A1 WO2002010834 A1 WO 2002010834A1 EP 0108412 W EP0108412 W EP 0108412W WO 0210834 A1 WO0210834 A1 WO 0210834A1
Authority
WO
WIPO (PCT)
Prior art keywords
microscope
slide
microscope stage
adapter
insert
Prior art date
Application number
PCT/EP2001/008412
Other languages
German (de)
English (en)
Inventor
Raimund SCHÜTZE
Original Assignee
P.A.L.M. Microlaser Technologies Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by P.A.L.M. Microlaser Technologies Ag filed Critical P.A.L.M. Microlaser Technologies Ag
Priority to AU2001282001A priority Critical patent/AU2001282001A1/en
Publication of WO2002010834A1 publication Critical patent/WO2002010834A1/fr

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/24—Base structure
    • G02B21/26—Stages; Adjusting means therefor

Definitions

  • the present invention relates to a microscope stage according to the preamble of claim 1.
  • a microscope stage which is part of a corresponding microscope, serves to hold a specimen slide, which in turn carries a material to be examined with the microscope.
  • a material to be examined with the microscope This can be, for example, a material that is to be examined with a microscope for chemical, biological or medical analyzes or experiments.
  • the microscope can be, for example, a microscope of the type described in WO 97/29355 A by the applicant, the microscope being coupled to a laser device in order to process individual biological and / or non-biological objects located on a slide and / or to be able to separate. It is proposed in this document to separate a selected biological object from the surrounding biological mass by means of a laser beam, so that the selected biological object is freely prepared from the surrounding biological mass. The thus freely prepared biological object is then catapulted from the slide to a collecting device with the aid of a laser shot, the collecting device being, for example, a collecting substrate or the like.
  • biological objects are understood to mean, above all, living or fixed biological cells or cell components which are part of a solid or liquid biological material, such as a cell tissue.
  • this method can also basically be applied to non-biological objects, ie inanimate matter, such as microscopic objects made of glass, silica, all kinds of polymers, etc.
  • the objective of the microscope is generally arranged above the slide, while the laser device is present below the slide.
  • the collecting device can, for example, be configured in such a way that it can be pivoted into the light path of the microscope or the laser, if necessary.
  • Microscope tables of conventional microscopes can generally be moved both horizontally, ie in the x and y directions, and vertically, ie in the z direction (for example with the aid of computer-controlled stepper motors). Furthermore, conventional microscope depressions have the respective slide in which they are placed. The area above the slide is freely accessible. After the slide has been inserted into the recess of the microscope table, the surface of the slide is several millimeters, for example 5 mm, below the surrounding surface of the microscope table. However, this also increases the Distance between the sample on the slide and the objective of the microscope or the collecting device described above. The sample can therefore not be moved anywhere near the objective of the microscope.
  • the collecting device must be lowered towards the slide in order to enable reliable collection of the objects catapulted away from the slide or the sample located thereon.
  • automatic feeding and removal of specimens or specimen slides is made more difficult, since the specimen slide has to be lowered into the recess of the microscope stage.
  • the present invention is therefore based on the object of proposing a microscope stage in which the disadvantages described above are eliminated and in particular an automatic supply and removal of specimen slides or specimens is facilitated.
  • the microscope stage according to the invention should advantageously be suitable for use in microscopes which have a laser device for catapulting out individual (biological or non-biological) objects from a specimen located on the slide and a collecting device for collecting and collecting the catapulted objects.
  • the microscope stage according to the invention should preferably be suitable for use in a large number of different microscopes.
  • the microscope stage according to the invention is equipped with a holding device for holding a specimen slide, the holding device being designed in such a way that the surface of the one located in the holding device Object carriers ' rs are arranged essentially flush with the surface of the microscope table surrounding the receiving device.
  • the receiving device can have a recess which is dimensioned such that the previously described flushness between the surface of the specimen slide and the surface of the microscope stage can be produced.
  • the depth of the recess can be, for example, in particular approximately 1-1.2 mm, so that conventional specimen slides, which have a thickness of approximately 1 mm, can be placed flush with the surface of the microscope table in the recess.
  • the receiving device or the depression described above is preferably designed in such a way that the placement of the specimen slide in the depression and the removal of the specimen slide is facilitated, wherein there is also a compromise with the simplest possible manufacture of the microscope table.
  • the receiving device has lateral depressions for holding the specimen slide in the receiving device, the depressions being spaced apart by a perforated area, which in particular is partially circular or segmented in the form of a segment, so that a portion of the perforated area protrudes laterally below the slide located in the accommodating device to easily grasp the in the
  • the receiving device is preferably in one in one
  • the surface of the Insert defines the surface of the microscope table described above, to which the surface of the slide located in the receiving device is arranged flush.
  • the microscope stage according to the invention Due to the design of the microscope stage according to the invention, i.e. The level between the surface of the specimen slide and the surrounding surface of the microscope table ensures that the specimen slide can be approached laterally more easily, so that the automation of the supply or removal of specimens for loading a specimen slide located in the receiving device and also the automation of the supply and discharge of the specimen slide itself is facilitated since an additional lifting movement for lowering the respective specimen or the respective specimen slide is not required.
  • the microscope stage according to the invention is also advantageous when used in a microscope with a previously described laser device and collecting device, since an additional lifting movement for lowering the collecting device can be omitted.
  • Sample that is on the slide can be moved closer to the microscope objective.
  • the microscope stage is provided with an exchangeable adapter for attaching the microscope stage to a tripod of a corresponding microscope, since this ensures that the microscope stage can be attached to different types of microscope only by exchanging the adapter ,
  • This feature which is in principle independent of the above-described recording device according to the invention, thus supports the universal applicability of the microscope stage for the most varied types of microscopes if a correspondingly extensive set of different different adapters for attaching the microscope stage to different microscope types is provided.
  • FIG. 1 shows a top view of an insert for a microscope stage according to a preferred exemplary embodiment of the present invention, and corresponding cross-sectional views of the insert,
  • FIG. 2 shows a bottom view of the insert shown in FIG. 1,
  • FIG. 3 shows various views of an adapter according to the invention for fastening a microscope stage to a microscope stand
  • FIG. 4 shows an exploded view of a microscope stage according to the preferred exemplary embodiment of the present invention
  • 5-FIG. 7 show perspective individual part representations of different slide parts of the microscope table shown in FIG. 4, and
  • FIG. 8 shows a partial cross-sectional view of the insert shown in FIG. 1 and FIG. 2 with a slide located therein.
  • FIG. 4 shows an exploded view of a microscope table according to the invention in accordance with a preferred exemplary embodiment.
  • the microscope stage essentially comprises a circular one Insert 1, a slide upper part 2, a slide middle part 3, a slide lower part 4 and an adapter 5 for fastening the microscope stage to a corresponding microscope stand. All components 1-5 can be made of aluminum, for example.
  • the insert 1 is shown in more detail in FIG. 1 and FIG. 2, a top view with corresponding cross-sectional views being shown in FIG. 1 and a bottom view of the insert 1 in FIG.
  • a recess corresponding to the elongated shape of the specimen slide is provided in the insert 1, said recess comprising two lateral depressions 6 formed in the upper side of the insert 1.
  • an opening 7 which extends from the top of the insert 1 to its underside and which ensures that a slide held in the recesses 6 can be illuminated and viewed both from above and from below.
  • the opening 7 has, in particular, circular or segment-like sections 9 and 10, which enable a specimen slide to be simply placed in the depressions 6 or a specimen slide placed in the depressions 6 to be gripped easily.
  • the radius of these circular segment-like sections 9 and 10 is selected such that the corresponding sections 9 and 10 protrude laterally beyond the edge of the slide placed in the recesses 6.
  • the depressions ⁇ form webs on both sides which are provided as supports or supports for the specimen slide. It can also be seen from FIG. 1 and FIG. 2 that a corresponding web also runs along the edge of the opening 7, so that a receiving device for the specimen slide is formed by the circumferential web or the depressions 6.
  • a further depression 8 of larger diameter is formed, which ensures that not only from above but also from below can be approached as close as possible to the slide with a corresponding objective.
  • the dimensions of the depressions 8 are chosen such that the freedom of movement with respect to the displacement of the table in the x and y directions is not restricted.
  • Bores 11 can be provided both on the bottom and on the top of the insert 1, which enable the insert 1 to be fastened or fixed in the upper slide part 2 shown in FIG.
  • FIG. 8 shows a cross-sectional view of the insert 1, corresponding to the cross-sectional views shown in FIG. 1, with a slide 12 located in the recesses 6.
  • the depth of the depressions 6 is chosen such that the surface of the specimen slide 12 is essentially flush with the surrounding surface of the insert 1, as is also shown in FIG.
  • the flushness shown in FIG. 8 can thus be achieved with a slide 12 with a thickness of 1 mm.
  • the recess 8 also shown in FIGS. 1, 2 and 8 can have a depth of approximately 2-3 mm, preferably 2.7 mm, for example.
  • FIG. 8 shows a partial cross-sectional view of the insert 1 in its working position, ie when the insert 1 is viewed from the front, while in FIG. 1 a partial cross-sectional view of the insert 1 along the in FIG Figure 1 shown section line AA is shown looking in the direction of the arrows shown.
  • FIG. 5 shows a perspective underside view of the upper carriage part 2. It can be seen from FIG. 5 that a recess 14 with a significantly larger diameter than the opening 12 is formed on the underside of the upper slide part 2.
  • An arm 15 with a threaded bore 16 is configured on one side of the upper slide part 2.
  • FIG. 6 shows a perspective underside view of the middle part 3 of the slide shown in FIG.
  • the middle part 3 of the slide has an opening 17 into which the arm 15 of the upper part 2 of the slide 2, which is shown in FIG. 5, is to be inserted, so that the threaded bore 16 also shown in FIG Threaded bores 22 and 23 are formed.
  • a threaded spindle is to be guided, one end of which is supported in the projection 19, while the other end comes to lie between the projection 20 and a further projection 21.
  • this end can be coupled to, for example, a computer-controlled stepper motor, in order to thus adjust the upper carriage part 2 in relation to the central carriage part 3 in the y direction.
  • rails 25 are formed on the upper side of the middle part of the slide, with provided on the side of the carriage upper part treads 26 are moved along these rails 25 when adjusting the (not shown) threaded spindle.
  • FIG. 7 shows a perspective underside view of the lower part 4 of the slide shown in FIG.
  • the lower carriage part 4 also has an opening 28 and on its underside projections 29-31, threaded bores being provided in the projections 29 and 30, through which a threaded spindle 35 shown in FIG. 4 is to be guided.
  • an element 36 sits firmly on the threaded spindle 35, which element is to be screwed to a projection 27 shown in FIG. 6 and formed on the underside of the middle part 3 of the slide.
  • the projection 31 formed on the underside of the lower part 4 of the slide has an opening 34, via which - as shown in FIG.
  • stepper motor 37 can be coupled to the threaded spindle 35 in order to adjust the threaded spindle accordingly.
  • Rails 38 are formed on the upper side of the lower part 4 of the slide, against which running surfaces 18, which are provided on the underside of the central part 3 of the slide (see FIG. 6), rest.
  • the lower part 4 of the slide has an adapter 5, which is used to fasten the lower part 4 of the slide (and thus the entire microscope stage) to a stand of a corresponding microscope.
  • the adapter 5 is preferably designed to be detachable from the lower part 4 of the slide and has bores 39 which allow the adapter or the lower part 4 of the slide to be fastened to the desired microscope stand. possible.
  • the adapter 5 can, for example, only be plugged onto the lower part 4 of the slide and then screwed to the corresponding microscope stand.
  • FIG. 3 shows various views of an adapter 5 according to a further exemplary embodiment of the present invention, a top view of the side facing the lower part 4 of the slide and also the top and the transverse side of the adapter 5 being shown. From Figure 3 it can be seen that, on the one hand
  • Drill holes 39 run from the front to the back and on the other hand drill holes 40 run from the top to the bottom of the adapter 5.
  • recesses 41 are provided, into which plug pins can be fitted, which in turn are to be inserted into corresponding drill holes in the lower part 4 of the slide, in order to couple the entire adapter 5 to the lower part of the slide.
  • the adapter 5 can then be fastened to the microscope stand of the desired microscope with the lower part of the slide or with the entire microscope stage via the drill holes 39 and 40.

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

L'invention concerne une table à microscope pouvant être employée universellement pour tous types de microscopes. Ladite table présente un dispositif de réception (6) destiné à recevoir un porte-objet (12), ledit dispositif de réception (6) étant conçu de manière que la surface du porte-objet (12) logé dans le dispositif de réception (6) est disposée de façon essentiellement jointive par rapport à la surface de la table à microscope entourant le dispositif de réception (6). Ledit dispositif de réception (6) est de préférence conçu sous forme d'insert interchangeable (1).
PCT/EP2001/008412 2000-07-31 2001-07-20 Table a microscope WO2002010834A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001282001A AU2001282001A1 (en) 2000-07-31 2001-07-20 Microscope table

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10037203.1 2000-07-31
DE2000137203 DE10037203C1 (de) 2000-07-31 2000-07-31 Mikroskoptisch

Publications (1)

Publication Number Publication Date
WO2002010834A1 true WO2002010834A1 (fr) 2002-02-07

Family

ID=7650793

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/008412 WO2002010834A1 (fr) 2000-07-31 2001-07-20 Table a microscope

Country Status (3)

Country Link
AU (1) AU2001282001A1 (fr)
DE (1) DE10037203C1 (fr)
WO (1) WO2002010834A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005114104A1 (fr) * 2004-05-07 2005-12-01 P.A.L.M. Microlaser Technologies Ag Table de microscope et piece rapportee
US7848552B2 (en) 2004-05-11 2010-12-07 P.A.L.M. Microlaser Technologies Gmbh Method for processing a material by means of a laser irradiation and control system

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986001004A1 (fr) * 1984-07-18 1986-02-13 Hermann Mader Appareil de selectionnement pour objets, en particulier pour les fossiles
US4946266A (en) * 1987-09-16 1990-08-07 Ernst Leitz Wetzlar Gmbh Universal object holder for microscopes
WO1997029355A1 (fr) * 1996-02-05 1997-08-14 P.A.L.M. Gmbh Procede et dispositif de micro-injection sans contact, de tri et de production d'objets biologiques obtenus de maniere planaire, a l'aide de faisceaux laser
EP0896238A2 (fr) * 1997-08-06 1999-02-10 Carl Zeiss Jena GmbH Stéréomicroscope avec changeur d'échantillons

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE8220792U1 (de) * 1982-07-21 1982-10-14 Fa. Carl Zeiss, 7920 Heidenheim Tisch fuer durchlichtmikroskope
US4974952A (en) * 1988-03-31 1990-12-04 Focht Daniel C Live cell chamber for microscopes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986001004A1 (fr) * 1984-07-18 1986-02-13 Hermann Mader Appareil de selectionnement pour objets, en particulier pour les fossiles
US4946266A (en) * 1987-09-16 1990-08-07 Ernst Leitz Wetzlar Gmbh Universal object holder for microscopes
WO1997029355A1 (fr) * 1996-02-05 1997-08-14 P.A.L.M. Gmbh Procede et dispositif de micro-injection sans contact, de tri et de production d'objets biologiques obtenus de maniere planaire, a l'aide de faisceaux laser
EP0896238A2 (fr) * 1997-08-06 1999-02-10 Carl Zeiss Jena GmbH Stéréomicroscope avec changeur d'échantillons

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005114104A1 (fr) * 2004-05-07 2005-12-01 P.A.L.M. Microlaser Technologies Ag Table de microscope et piece rapportee
US7576912B2 (en) 2004-05-07 2009-08-18 P.A.L.M. Microlaser Technologies Gmbh Microscope table and insert
US7848552B2 (en) 2004-05-11 2010-12-07 P.A.L.M. Microlaser Technologies Gmbh Method for processing a material by means of a laser irradiation and control system

Also Published As

Publication number Publication date
AU2001282001A1 (en) 2002-02-13
DE10037203C1 (de) 2002-04-04

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