Classifications

• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B82—NANOTECHNOLOGY
  • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
  • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
  • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
  • A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
  • A61K47/40—Cyclodextrins; Derivatives thereof
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
  • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
  • A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
  • A61K47/6949—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit inclusion complexes, e.g. clathrates, cavitates or fullerenes
  • A61K47/6951—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit inclusion complexes, e.g. clathrates, cavitates or fullerenes using cyclodextrin
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K9/00—Medicinal preparations characterised by special physical form
  • A61K9/0012—Galenical forms characterised by the site of application
  • A61K9/0014—Skin, i.e. galenical aspects of topical compositions
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K9/00—Medicinal preparations characterised by special physical form
  • A61K9/0012—Galenical forms characterised by the site of application
  • A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/04—Antibacterial agents
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K9/00—Peptides having up to 20 amino acids, containing saccharide radicals and having a fully defined sequence; Derivatives thereof
  • C07K9/006—Peptides having up to 20 amino acids, containing saccharide radicals and having a fully defined sequence; Derivatives thereof the peptide sequence being part of a ring structure
  • C07K9/008—Peptides having up to 20 amino acids, containing saccharide radicals and having a fully defined sequence; Derivatives thereof the peptide sequence being part of a ring structure directly attached to a hetero atom of the saccharide radical, e.g. actaplanin, avoparcin, ristomycin, vancomycin
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K38/00—Medicinal preparations containing peptides

Definitions

• This invention is directed to novel polyacid derivatives of glycopeptide antibiotics and related compounds. This invention is also directed to pharmaceutical compositions containing such glycopeptide derivatives, methods of using such glycopeptide derivatives as antibacterial agents, and processes and intermediates useful for preparing such glycopeptide derivatives.
• Glycopeptides are a well-known class of antibiotics produced by various microorganisms (see Glycopeptide Antibiotics, edited by R. Nagarajan, Marcel Dekker, Inc. New York (1994)). These complex multi-ring peptide compounds are very effective antibacterial agents against a majority of Gram-positive bacteria. Although potent antibacterial agents, the glycopeptides antibiotics are not used in the treatment of bacterial diseases as often as other classes of antibiotics, such as the semi-synthetic penicillins, cephalosporins and lincomycins, due to concerns regarding toxicity.
• VRE vancomycin-resistant enterococci
• a number of derivatives of vancomycin and other glycopeptides are known in the art. For example, see U.S.
• Other derivatives are disclosed in EP 0 802 199; EP 0 801 075; EP 0 667 353; WO 97/28812; WO 97/38702; WO 98/52589; WO 98/52592; and in J Amer. Chem. Soc, 1996, 118, 13107-13108; J. Amer. Chem. Soc, 1997, 119, 12041-12047; and J Amer.
• glycopeptide derivatives having effective antibacterial activity and an improved mammalian safety profile.
• a need exists for glycopeptide derivatives which are effective against a wide spectrum of pathogenic microorganisms, including vancomycin-resistant microorganisms.
• the present invention provides novel polyacid glycopeptide derivatives having highly effective antibacterial activity. Accordingly, the invention provides a compound of the invention, which is a glycopeptide substituted at the C-terminus with a substituent that comprises two or more (e.g. 2, 3, 4, or 5) carboxy (CO 2 H) groups; or a pharmaceutically acceptable salt, or stereoisomer, or prodrug thereof.
• the substituent is attached to a carbonyl group of the C- terminus to form an amide bond, an ester bond, or a thioester bond. More preferably, the substituent is attached to a carbonyl group of the C-terminus to form an amide bond. More preferably, the substituent comprises two carboxy (CO 2 H) groups.
• Preferred substituents at the C-terminus include a nitrogen- linked aspartic acid or a nitrogen linked glutamic acid.
• the compounds of the invention may preferably exclude the glycopeptides 1) teicoplanin A2 substituted at the C-terminus with a nitrogen-linked glutamic acid, and 2) teicoplanin aglycon (TD) substituted at the C-terminus with a nitrogen-linked glutamic acid.
• glycopeptide derivatives are also described in U.S. Patent Application Serial Number 09/470,209, filed 22 December 1999. Accordingly, the compounds of the invention may also preferably exclude glycopeptides of formula II:
• the invention also preferably exclude glycopeptides of formula II: e) wherein R 17 is nitrogen-linked aspartic acid; R 18 is hydrogen; R 19 is hydrogen; R 20 is 2-[4-(4- chlorobenzyloxy)benzylamino]ethyl; and R 21 is hydrogen; f) wherein ⁇ R 17 is 5-(2-carboxypyrrolidin-l-ylcarbonyl)-5- (2-carboxy-3-phenylpropylamino)pentylamino; R 18 is hydrogen; R 19 is hydrogen; R 20 is 2-(decylamino)ethyl; and R 21 is hydrogen; g) wherein ⁇ R 17 is nitrogen-linked aspartic acid; R 18 is hydrogen; R 19 is hydrogen; R 20 is 2-(decylamino)ethyl; and R 21 is -CH 2 -N-(N-CH 3 -D-glucamine); h) wherein ⁇ R 17 is nitrogen-linked aspartic acid; R 18 is hydrogen;
• a preferred compound of the invention is a glycopeptide of formula I:
• R 1 is selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, aryl, heteroaryl, heterocyclic and -R a - Y-R b -(Z) X ; or R 1 is a saccharide group optionally substituted with -R a - Y-R b -(Z) X , R f , -C(O)R-, or -C(O)-R a - Y-R b -(Z) X ;
• R 2 is hydrogen or a saccharide group optionally substituted with -R a - Y-R b -(Z) X , R f , -C(O)R f , or -C(O)-R a -Y-R b -(Z) x ;
• R 3 is a nitrogen-linked, oxygen-linked, or sulfur-linked substituent comprising two or more carboxy groups
• R 4 is selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, -R a -Y-R b -(Z) X , -C(O)R d and a saccharide group optionally substituted with -R a - Y-R b -(Z) X , R f , -C(O)R f , or -C(O)-R a - Y-R b -(Z) X ;
• R 6 is selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, -R a -Y-R b -(Z) X , - C(O)R d and a saccharide group optionally substituted with -NR C -R a - Y-R b -(Z) X , or R 5 and R 6 can be joined,
• R 7 is selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, -R a -Y-R b -(Z) X , and -C(O)R d ;
• R 8 is selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, aryl, heteroaryl and heterocyclic;
• R 9 is selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, aryl, heteroaryl and heterocyclic;
• R 10 is selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, aryl, heteroaryl and heterocyclic; or R 8 and R 10 are joined to form -Ar'-O- Ar 2 -, where Ar 1 and Ar 2 are independently arylene or heteroarylene;
• R u is selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, aryl, heteroaryl and heterocyclic, or R 10 and R 11 are joined, together with the carbon and nitrogen atoms to which they are attached, to form a heterocyclic ring;
• R 12 is selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, aryl, heteroaryl, heterocyclic, -C(O)R d , -C(NH)R d , -C(O)NR c R c , -C(O)OR d , -C(NH)NR C R C and -R a - Y-R b -(Z) X , or R u and R 12 are joined, together with the nitrogen atom to which they are attached, to form a heterocyclic ring; R 13 is selected from the group consisting of hydrogen or -OR 14 ;
• R 14 is selected from hydrogen, -C(O)R d and a saccharide group; each R a is independently selected from the group consisting of alkylene, substituted alkylene, alkenylene, substituted alkenylene, alkynylene and substituted alkynylene; each R b is independently selected from the group consisting of a covalent bond, alkylene, substituted alkylene, alkenylene, substituted alkenylene, alkynylene and substituted alkynylene, provided R b is not a covalent bond when Z is hydrogen; each R c is independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, aryl, heteroaryl, heterocyclic and -C(O)R d ; each R
• R e is a saccharide group; each R f is independently alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, aryl, heteroaryl, or heterocyclic;
• R x is an N-linked amino saccharide or an N-linked heterocyclic
• X 1 , X 2 and X 3 are independently selected from hydrogen or chloro; each Y is independently selected from the group consisting of oxygen, sulfur, -S-S-, -NR C -, -S(O)-, -SO 2 -, -NR c C(O)-, -OSO 2 -, -OC(O)-, -NR c SO 2 -, -C(O)NR c -, -C(O)O-, -SO 2 NR c -, -SO 2 O-, -P(O)(OR c )O-, -P(O)(OR c )NR c -, -OP(O)(OR c )O-,-OP(O)(OR c )NR c -, -OC(O)O-, -NR c C(O)O-, -NR c C(O)NR c -, -OC(O)NR c -
• R 1 is a saccharide group optionally substituted with -R a - Y-R b -(Z) X , R f , -C(O)R f , or -C(O)-R a -Y-R b -(Z). More preferably R 1 is an amino saccharide group substituted on the saccharide nitrogen with - CH 2 CH 2 -NH- (CH 2 ) 9 CH 3 ; - CH 2 CH 2 CH 2 -NH- (CH 2 ) 8 CH 3 ; -CH 2 CH 2 CH 2 CH 2 -NH-(CH 2 ) 7 CH 3 ; -CH 2 CH 2 -NHSO 2 -(CH 2 ) 9 CH 3 ;
• R 1 is also an amino saccharide group substituted on the saccharide nitrogen with a 4-(4- chlorophenyl)benzyl group or with a 4-(4-chlorobenzyloxy)benzyl group.
• R 1 can also be a saccharide group of the formula:
• R 15 is -R a - Y-R b -(Z) X , R f , -C(O)R f , or -C(O)-R a - Y-R b -(Z) X ; and R 16 is hydrogen or methyl.
• R 2 is hydrogen.
• the substituent R 3 is attached to the C-terminus to form an amide bond, an ester bond, or a thioester bond. More preferably, R 3 is attached to the C-terminus to form an amide bond. More preferably, the substituent R 3 comprises two carboxy (CO 2 H) groups.
• Preferred R 3 substituents include a nitrogen-linked aspartic acid or a nitrogen linked glutamic acid.
• R 3 can also be a nitrogen-linked radical of formula III:
• R g is a saccharide group. More preferably, R 8 is N-(N-methyl-D- glucamine) or N-(D-glucosamine).
• R 4 , R 6 and R 7 are each independently selected from hydrogen or - C(O)R d . More preferably, R 4 , R 6 and R 7 are each hydrogen.
• R 5 is hydrogen, -CH 2 -NHR C , -CH 2 -NR c R e or -CH 2 -NH-R a - Y-R b -(Z) X .
• R 5 can also preferably be hydrogen; -CH 2 -N-(N- CH 3 -D-glucamine); -CH 2 - ⁇ H-CH 2 CH 2 - ⁇ H-(CH 2 ) 9 CH 3 ; -CH 2 -NH-CH 2 CH 2 - NHC(O)-(CH 2 ) 3 COOH; -CH 2 -NH-(CH 2 ) 9 CH 3 ;-CH 2 -NH-CH 2 CH 2 -COOH; -CH 2 - NH-(CH 2 ) 5 COOH; -CH 2 -(morpholin-4-yl); -CH 2 -NH-CH 2 CH 2 -O-CH 2 CH 2 OH; - CH 2 -NH-CH 2 CH(OH)-CH 2 OH; -CH 2 -N[CH 2 CH 2 OH] 2 ; -CH 2 -NH-(CH 2 ) 3 - N(CH 3 ) 2 ; -CH 2 -N[(CH 2 ) 3
• R 8 is -CH 2 C(O)NH 2 , -CH 2 COOH, benzyl, 4-hydroxyphenyl or 3-chloro-4-hydroxyphenyl.
• R 9 is hydrogen or alkyl.
• R 10 is alkyl or substituted alkyl. More preferably, R 10 is the side-chain of a naturally occurring amino acid, such as isobutyl.
• R 11 is hydrogen or alkyl.
• R 12 is hydrogen, alkyl, substituted alkyl or -C(O)R d .
• R 12 can also preferably be hydrogen; -CH 2 COOH; -CH 2 -[CH(OH)] 5 CH 2 OH; -
• X 3 is hydrogen
• each Y is independently selected from the group consisting of oxygen, sulfur, -S-S-, -NR C -, -S(O)-, -SO 2 -, -NR c C(O)-, -OSO 2 -, -OC(O)-, -NR c SO 2 -, -C(O)NR c -, -C(O)O-, -SO 2 NR c -, -SO 2 O-, -P(O)(OR c )O- , -P(O)(OR c )NR c - , - OP(O)(OR c )O- ,- OP(O)(OR c )NR c - ,
• n is 0 or 1 , and more preferably, n is 1.
• Another preferred compound of the invention is a glycopeptide of formula II:
• R is a dicarboxy-substituted alkyl group having from 3 to 10 carbon atoms
• R 18 is selected from the group consisting of hydrogen and alkyl
• R 19 is hydrogen
• R 20 is -R a -Y-R b -(Z) X ;
• R 21 is hydrogen
• R a is selected from the group consisting of alkylene, substituted alkylene, alkenylene, substituted alkenylene, alkynylene and substituted alkynylene;
• R b is selected from the group consisting of a covalent bond, alkylene, substituted alkylene, alkenylene, substituted alkenylene, alkynylene and substituted alkynylene, provided R b is not a covalent bond when Z is hydrogen;
• Y is selected from the group consisting of sulfur, -S(O)- and -SO 2 -; each Z is independently selected from hydrogen, aryl, cycloalkyl, cycloalkenyl, heteroaryl and heterocyclic; and x is 1 or 2; or a pharmaceutically acceptable salt, or stereoisomer, or prodrug thereof.
• R a is preferably selected from alkylene having from 1 to 10 carbon atoms.
• R can be ethylene (-CH 2 CH 2 -), propylene (-CH 2 CH 2 CF£ 2 -) or butylene (-CH 2 CH 2 CH 2 CH 2 -).
• Z is preferably hydrogen and R b is alkylene of from 8 to 12 carbon atoms. More preferably, R b can be a covalent bond, methylene, -(CH 2 ) 6 -, -(CH 2 ) 7 -, -(CH 2 ) 8 -, -(CH 2 ) 9 - or -(CH 2 ) 10 -. Additionally, R b and Z can preferably form an w-octyl, n-nonyl, n-decyl, n- undecyl or «-dodecyl group.
• Z is preferably aryl, cycloalkyl, cycloalkenyl, heteroaryl and heterocyclic, and R b is a covalent bond or alkylene of from 1 to 10 carbon atoms. More preferably, Z can be aryl.
• Preferred aryl groups include phenyl, substituted phenyl, biphenyl, substituted biphenyl and terphenyl groups.
• Z can be phenyl, 4-isobutylphenyl, 4'- chlorobiphenyl-4-yl, 4'-trifluoromethylbiphenyl-4-yl, 4-(naphth-2-yl)phenyl, 4- (2-phenylethynyl)phenyl, 4-(3,4-dichlorobenzyloxy)-phenyl, or -terphenyl.
• x is preferably 1.
• Y is preferably sulfur.
• -R a - Y-R b -(Z) X is preferably selected from the group consisting of: -CH 2 CH 2 -S-(CH 2 ) 8 CH 3 ; -CH 2 CH 2 -S-(CH 2 ) 9 CH 3 ; -CH 2 CH 2 -S-(CH ) 10 CH 3 ;
• -R a -Y-R b -(Z) X is -CH 2 CH 2 -S-(CH 2 ) 9 CH 3 .
• R 17 is preferably a dicarboxy-substituted alkyl group having from 4 to 6 carbon atoms. More preferably R 17 is-
• R 17 is -CH 2 (COOH)CH 2 COOH.
• R 18 is preferably hydrogen.
• a preferred compound of formula II is a compound wherein -R a -Y-R b -(Z) X is -CH 2 CH 2 -S-(CH 2 ) 9 CH 3 ; R 17 is -CH 2 (COOH)CH 2 COOH; and R 18 is hydrogen.
• R 15 , R 20 , or -R a - Y-R b -(Z) X is -CH 2 CH 2 -NH-(CH 2 ) 9 CH 3 ; -CH 2 CH 2 CH 2 -NH-(CH 2 ) 8 CH 3 ;
• R 15 or R 20 is 4-(4-chlorophenyl)benzyl or 4-(4-chlorobenzyloxy)benzyl.
• the invention also provides a pharmaceutical composition
• a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a therapeutically effective amount of a compound of the invention.
• the pharmaceutically acceptable carrier comprises an aqueous cyclodextrin solution.
• the cyclodextrin is hydroxypropyl- ⁇ -cyclodextrin or sulfobutyl ether ⁇ -cyclodextrin. More preferably, the cyclodextrin is hydroxypropyl- ⁇ -cyclodextrin.
• the compounds of the invention are highly effective antibacterial agents. Accordingly, the invention also provides a method of treating a mammal having a bacterial disease, comprising administering to the mammal a therapeutically effective amount of a compound of the invention. The invention also provides a method of treating a mammal having a bacterial disease, comprising administering to the mammal a therapeutically effective amount of a pharmaceutical composition of the invention.
• the invention also provides processes and intermediates useful for preparing compounds of the invention, which processes and intermediates are described further herein.
• the invention also provides a compound of the invention as described herein for use in medical therapy, as well as the use of a compound of the invention in the manufacture of a formulation or medicament for treating a bacterial disease in a mammal.
• Preferred compounds of the invention are the compounds of formula II shown in Table I below wherein R 19 and R 21 are each hydrogen.
• Table T Preferred Compounds of Formula TT
• Another preferred group of compounds of the invention are C-terminus polyacid derivatives of the glycopeptide antibiotic A82846B (also known as chloroorienticin A oy LY264826). See for example R. Nagarajan et al., J. Org. Chem., 1988, 54, 983-986; and N. Tsuji et al., J. Antibiot., 1988, 41, 819-822.
• the structure of this glycopeptide is similar to vancomycin, except A82846B contains an additional amino sugar (i.e.
• a preferred group of compounds are N-alkylated derivatives of A82846B having a polyacid group at the C-terminus.
• N-alkylated derivatives of A82846B are known in the art and are described, for example, in U.S. Patent Number 5,840,684.
• C-Terminus polyacid derivatives of these compounds can readily be prepared using the procedures described herein.
• a particularly preferred group of compounds of the invention are A82846B derivatives having a 4-(4-chlorophenyl)benzyl group or a 4-(4- chlorobenzyloxy)benzyl group attached at the amino group of the 4-epi- vancosamine of the disaccharide moiety and having a group substituted at the C- terminus that comprises two or more carboxy group.
• Preferred polycarboxy substituents for A82846B derivatives include a nitrogen-linked aspartic acid, a nitrogen linked glutamic acid, and a nitrogen-linked radical of formula III as described herein.
• This invention relates to novel derivatives of glycopeptide antibiotics comprising a polyacid containing group at the C-terminus (compounds of the invention), as well as to compositions comprising such compounds and to therapeutic methods comprising the administration of such compounds.
• compounds of the invention the following terms have the following meanings, unless otherwise indicated. Definitions
• alkyl refers to a monoradical branched or unbranched saturated hydrocarbon chain preferably having from 1 to 40 carbon atoms, more preferably 1 to 10 carbon atoms, and even more preferably 1 to 6 carbon atoms. This term is exemplified by groups such as methyl, ethyl, n-propyl, wo-propyl, n-butyl, w-hexyl, w-decyl, tetradecyl, and the like.
• substituted alkyl refers to an alkyl group as defined above, having from 1 to 8 substituents, preferably 1 to 5 substitutents, and more preferably 1 to 3 substituents, selected from the group consisting of alkoxy, substituted alkoxy, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, acyl, acylamino, acyloxy, amino, substituted amino, aminoacyl, aminoacyloxy, oxyaminoacyl, azido, cyano, guanido, halogen, hydroxyl, keto, thioketo, carboxy, carboxyalkyl, thioaryloxy, thioheteroaryloxy, thioheterocyclooxy, thiol, thioalkoxy, substituted thioalkoxy, aryl, aryloxy, heteroaryl, heteroaryloxy, heterocyclic, heterocyclo
• alkylene refers to a diradical of a branched or unbranched saturated hydrocarbon chain, preferably having from 1 to 40 carbon atoms, preferably 1-10 carbon atoms, more preferably 1-6 carbon atoms. This term is exemplified by groups such as methylene (-CH 2 -), ethylene (-CH 2 CH 2 -), the propylene isomers (e.g., -CH 2 CH 2 CH 2 - and -CH(CH 3 )CH 2 -) and the like.
• substituted alkylene refers to an alkylene group, as defined above, having from 1 to 5 substituents, and preferably 1 to 3 substituents, selected from the group consisting of alkoxy, substituted alkoxy, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, acyl, acylamino, acyloxy, amino, substituted amino, aminoacyl, aminoacyloxy, oxyaminoacyl, azido, cyano, halogen, hydroxyl, carboxy, carboxyalkyl, thioaryloxy, thioheteroaryloxy, thioheterocyclooxy, thiol, thioalkoxy, substituted thioalkoxy, aryl, aryloxy, heteroaryl, heteroaryloxy, heterocyclic, heterocyclooxy, hydroxyamino, alkoxyamino, nitro, -SO-alky
• substituted alkylene groups include those where 2 substituents on the alkylene group are fused to form one or more cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, aryl, heterocyclic or heteroaryl groups fused to the alkylene group.
• fused groups Preferably contain from 1 to 3 fused ring structures.
• substituted alkylene includes alkylene groups in which from 1 to 5 of the alkylene carbon atoms are replaced with oxygen, sulfur or -NR- where R is hydrogen or alkyl.
• substituted alkylenes are chloromethylene (-CH(Cl)-), aminoethylene (-CH(NH 2 )CF£2-), 2-carboxypropylene isomers (-CH 2 CH(CO 2 H)CH 2 -), ethoxyethyl (-CH 2 CH 2 O-CH 2 CH 2 -) and the like.
• alkaryl refers to the groups -alkylene-aryl and -substituted alkylene-aryl where alkylene, substituted alkylene and aryl are defined herein. Such alkaryl groups are exemplified by benzyl, phenethyl and the like.
• alkoxy refers to the groups alkyl-O-, alkenyl-O-, cycloalkyl- O-, cycloalkenyl-O-, and alkynyl-O-, where alkyl, alkenyl, cycloalkyl, cycloalkenyl, and alkynyl are as defined herein.
• Preferred alkoxy groups are alkyl-O- and include, by way of example, methoxy, ethoxy, n-propoxy, is o- propoxy, -butoxy, tert-butoxy, .sec-butoxy, n-pentoxy, w-hexoxy, 1,2- dimethylbutoxy, and the like.
• substituted alkoxy refers to the groups substituted alkyl-O-, substituted alkenyl-O-, substituted cycloalkyl-O-, substituted cycloalkenyl-O-, and substituted alkynyl-O- where substituted alkyl, substituted alkenyl, substituted cycloalkyl, substituted cycloalkenyl and substituted alkynyl are as defined herein.
• alkylalkoxy refers to the groups -alkylene-O-alkyl, alkylene-O-substituted alkyl, substituted alkylene-O-alkyl and substituted alkylene-O-substituted alkyl wherein alkyl, substituted alkyl, alkylene and substituted alkylene are as defined herein.
• Preferred alkylalkoxy groups are alkylene-O-alkyl and include, by way of example, methylenemethoxy (- CH 2 OCH 3 ), ethylenemethoxy (-CH 2 CH 2 OCH 3 ), «-propylene-wo-propoxy (- CH 2 CH 2 CH 2 OCH(CH 3 ) 2 ), methylene-t-butoxy (-CH 2 -O-C(CH 3 ) 3 ) and the like.
• alkylthioalkoxy refers to the group -alkylene-S-alkyl, alkylene-S-substituted alkyl, substituted alkylene-S-alkyl and substituted alkylene-S-substituted alkyl wherein alkyl, substituted alkyl, alkylene and substituted alkylene are as defined herein.
• Preferred alkylthioalkoxy groups are alkylene-S-alkyl and include, by way of example, methylenethiomethoxy (- CH 2 SCH 3 ), ethylenethiomethoxy (-CH 2 CH 2 SCH 3 ), «-propylene-.s ⁇ -thiopropoxy (-CH 2 CH 2 CH 2. SCH(CH 3 ) 2 ), methylene-t-thiobutoxy (-CH 2 SC(CH 3 ) 3 ) and the like.
• alkenyl refers to a monoradical of a branched or unbranched unsaturated hydrocarbon group preferably having from 2 to 40 carbon atoms, more preferably 2 to 10 carbon atoms and even more preferably 2 to 6 carbon atoms and having at least 1 and preferably from 1-6 sites of vinyl unsaturation.
• substituted alkenyl refers to an alkenyl group as defined above having from 1 to 5 substituents, and preferably 1 to 3 substituents, selected from the group consisting of alkoxy, substituted alkoxy, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, acyl, acylamino, acyloxy, amino, substituted amino, aminoacyl, aminoacyloxy, oxyaminoacyl, azido, cyano, halogen, hydroxyl, keto, thioketo, carboxy, carboxyalkyl, thioaryloxy, thioheteroaryloxy, thioheterocyclooxy, thiol, thioalkoxy, substituted thioalkoxy, aryl, aryloxy, heteroaryl, heteroaryloxy, heterocyclic, heterocyclooxy, hydroxyamino, alkoxyamino,
• alkenylene refers to a diradical of a branched or unbranched unsaturated hydrocarbon group preferably having from 2 to 40 carbon atoms, more preferably 2 to 10 carbon atoms and even more preferably 2 to 6 carbon atoms and having at least 1 and preferably from 1-6 sites of vinyl unsaturation.
• substituted alkenylene refers to an alkenylene group as defined above having from 1 to 5 substituents, and preferably from 1 to 3 substituents, selected from the group consisting of alkoxy, substituted alkoxy, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, acyl, acylamino, acyloxy, amino, substituted amino, aminoacyl, aminoacyloxy, oxyaminoacyl, azido, cyano, halogen, hydroxyl, carboxy, carboxyalkyl, thioaryloxy, thioheteroaryloxy, thioheterocyclooxy, thiol, thioalkoxy, substituted thioalkoxy, aryl, aryloxy, heteroaryl, heteroaryloxy, heterocyclic, heterocyclooxy, hydroxyamino, alkoxyamino, nitro, -SO-al
• substituted alkenylene groups include those where 2 substituents on the alkenylene group are fused to form one or more cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, aryl, heterocyclic or heteroaryl groups fused to the alkenylene group.
• alkynyl refers to a monoradical of an unsaturated hydrocarbon preferably having from 2 to 40 carbon atoms, more preferably 2 to 20 carbon atoms and even more preferably 2 to 6 carbon atoms and having at least 1 and preferably from 1-6 sites of acetylene (triple bond) unsaturation.
• Preferred alkynyl groups include ethynyl (-C ⁇ CH), propargyl (-CH 2 C ⁇ CH) and the like.
• substituted alkynyl refers to an alkynyl group as defined above having from 1 to 5 substituents, and preferably 1 to 3 substituents, selected from the group consisting of alkoxy, substituted alkoxy, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, acyl, acylamino, acyloxy, amino, substituted amino, aminoacyl, aminoacyloxy, oxyaminoacyl, azido, cyano, halogen, hydroxyl, carboxy, carboxyalkyl, thioaryloxy, thioheteroaryloxy, thioheterocyclooxy, thiol, thioalkoxy, substituted thioalkoxy, aryl, aryloxy, heteroaryl, heteroaryloxy, heterocyclic, heterocyclooxy, hydroxyamino, alkoxyamino, nitro, -SO-
• alkynylene refers to a diradical of an unsaturated hydrocarbon preferably having from 2 to 40 carbon atoms, more preferably 2 to 10 carbon atoms and even more preferably 2 to 6 carbon atoms and having at least 1 and preferably from 1-6 sites of acetylene (triple bond) unsaturation.
• Preferred alkynylene groups include ethynylene (-C ⁇ C-), propargylene (-CH 2 C ⁇ C-) and the like.
• substituted alkynylene refers to an alkynylene group as defined above having from 1 to 5 substituents, and preferably 1 to 3 substituents, selected from the group consisting of alkoxy, substituted alkoxy, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, acyl, acylamino, acyloxy, amino, substituted amino, aminoacyl, aminoacyloxy, oxyaminoacyl, azido, cyano, halogen, hydroxyl, keto, thioketo, carboxy, carboxyalkyl, thioaryloxy, thioheteroaryloxy, thioheterocyclooxy, thiol, thioalkoxy, substituted thioalkoxy, aryl, aryloxy, heteroaryl, heteroaryloxy, heterocyclic, heterocyclooxy, hydroxyamino, alkoxyamino
• acyl refers to the groups HC(O)-, alkyl-C(O)-, substituted alkyl-C(O)-, cycloalkyl-C(O)-, substituted cycloalkyl-C(O)-, cycloalkenyl-C(O)- , substituted cycloalkenyl-C(O)-, aryl-C(O)-, heteroaryl-C(O)- and heterocyclic- C(O)- where alkyl, substituted alkyl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, aryl, heteroaryl and heterocyclic are as defined herein.
• acylamino or “aminocarbonyl” refers to the group -C(O)NRR where each R is independently hydrogen, alkyl, substituted alkyl, aryl, heteroaryl, heterocyclic or where both R groups are joined to form a heterocyclic group (e.g., morpholino) wherein alkyl, substituted alkyl, aryl, heteroaryl and heterocyclic are as defined herein.
• aminoacyl refers to the group -NRC(O)R where each R is independently hydrogen, alkyl, substituted alkyl, aryl, heteroaryl, or heterocyclic wherein alkyl, substituted alkyl, aryl, heteroaryl and heterocyclic are as defined herein.
• aminoacyloxy or “alkoxycarbonylamino” refers to the group -NRC(O)OR where each R is independently hydrogen, alkyl, substituted alkyl, aryl, heteroaryl, or heterocyclic wherein alkyl, substituted alkyl, aryl, heteroaryl and heterocyclic are as defined herein.
• acyloxy refers to the groups alkyl-C(O)O-, substituted alkyl- C(O)O-, cycloalkyl-C(O)O-, substituted cycloalkyl-C(O)O-, aryl-C(O)O-, heteroaryl-C(O)O-, and heterocyclic-C(O)O- wherein alkyl, substituted alkyl, cycloalkyl, substituted cycloalkyl, aryl, heteroaryl, and heterocyclic are as defined herein.
• aryl refers to an unsaturated aromatic carbocyclic group of from 6 to 20 carbon atoms having a single ring (e.g., phenyl) or multiple condensed (fused) rings, wherein at least one ring is aromatic (e.g., naphthyl, dihydrophenanthrenyl, fluorenyl, or anthryl).
• Preferred aryls include phenyl, naphthyl and the like.
• such aryl groups can optionally be substituted with from 1 to 5 substituents, preferably 1 to 3 substituents, selected from the group consisting of acyloxy, hydroxy, thiol, acyl, alkyl, alkoxy, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, substituted alkyl, substituted alkoxy, substituted alkenyl, substituted alkynyl, substituted cycloalkyl, substituted cycloalkenyl, amino, substituted amino, aminoacyl, acylamino, alkaryl, aryl, aryloxy, azido, carboxy, carboxyalkyl, cyano, halo, nitro, heteroaryl, heteroaryloxy, heterocyclic, heterocyclooxy, aminoacyloxy, oxyacylamino, sulfonamide, thioalkoxy, substituted thioalkoxy,
• aryloxy refers to the group aryl-O- wherein the aryl group is as defined above including optionally substituted aryl groups as also defined above.
• arylene refers to the diradical derived from aryl (including substituted aryl) as defined above and is exemplified by 1 ,2-phenylene, 1,3- phenylene, 1 ,4-phenylene, 1 ,2-naphthylene and the like.
• amino refers to the group -NH 2 .
• substituted amino refers to the group -NRR where each R is independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, cycloalkyl, substituted cycloalkyl, alkenyl, substituted alkenyl, cycloalkenyl, substituted cycloalkenyl, alkynyl, substituted alkynyl, aryl, heteroaryl and heterocyclic provided that both R's are not hydrogen.
• Amino acid refers to any of the naturally occurring amino acids (e.g.
• side chains of naturally occurring amino acids include, for example, hydrogen (e.g., as in glycine), alkyl (e.g., as in alanine, valine, leucine, isoleucine, proline), substituted alkyl (e.g., as in threonine, serine, methionine, cysteine, aspartic acid, asparagine, glutamic acid, glutamine, arginine, and lysine), alkaryl (e.g., as in phenylalanine and tryptophan), substituted arylalkyl (e.g., as in tyrosine), and heteroarylalkyl (e.g., as in histidine).
• alkyl e.g., as in alanine, valine, leucine, isoleucine, proline
• substituted alkyl e.g., as in threonine, serine, methionine, cysteine, aspartic acid, as
• C-terminus as it relates to a glycopeptide is well understood in the art.
• the C-terminus is the position substituted by the group R 3 .
• dicarboxy-substituted alkyl refers to an alkyl group substituted with two carboxy groups. This term includes, by way of example, -CH 2 (COOH)-CH 2 COOH and -CH 2 (COOH)CH 2 CH 2 COOH.
• carboxyalkyl or “alkoxycarbonyl” refers to the groups “-C(O)O-alkyl", “-C(O)O-substituted alkyl", “-C(O)O-cycloalkyl", “-C(O)O- substituted cycloalkyl", “-C(O)O-alkenyl”, “-C(O)O-substituted alkenyl", “- C(O)O-alkynyl” and "-C(O)O-substituted alkynyl” where alkyl, substituted alkyl, cycloalkyl, substituted cycloalkyl, alkenyl, substituted alkenyl, alkynyl and substituted alkynyl alkynyl are as defined herein.
• cycloalkyl refers to cyclic alkyl groups of from 3 to 20 carbon atoms having a single cyclic ring or multiple condensed rings.
• Such cycloalkyl groups include, by way of example, single ring structures such as cyclopropyl, cyclobutyl, cyclopentyl, cyclooctyl, and the like, or multiple ring structures such as adamantanyl, and the like.
• substituted cycloalkyl refers to cycloalkyl groups having from 1 to 5 substituents, and preferably 1 to 3 substituents, selected from the group consisting of alkoxy, substituted alkoxy, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, acyl, acylamino, acyloxy, amino, substituted amino, aminoacyl, aminoacyloxy, oxyaminoacyl, azido, cyano, halogen, hydroxyl, keto, thioketo, carboxy, carboxyalkyl, thioaryloxy, thioheteroaryloxy, thioheterocyclooxy, thiol, thioalkoxy, substituted thioalkoxy, aryl, aryloxy, heteroaryl, heteroaryloxy, heterocyclic, heterocyclooxy, hydroxyamino, alkoxyamino,
• cycloalkenyl refers to cyclic alkenyl groups of from 4 to 20 carbon atoms having a single cyclic ring and at least one point of internal unsaturation.
• suitable cycloalkenyl groups include, for instance, cyclobut-2-enyl, cyclopent-3-enyl, cyclooct-3-enyl and the like.
• substituted cycloalkenyl refers to cycloalkenyl groups having from 1 to 5 substituents, and preferably 1 to 3 substituents, selected from the group consisting of alkoxy, substituted alkoxy, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, acyl, acylamino, acyloxy, amino, substituted amino, aminoacyl, aminoacyloxy, oxyaminoacyl, azido, cyano, halogen, hydroxyl, keto, thioketo, carboxy, carboxyalkyl, thioaryloxy, thioheteroaryloxy, thioheterocyclooxy, thiol, thioalkoxy, substituted thioalkoxy, aryl, aryloxy, heteroaryl, heteroaryloxy, heterocyclic, heterocyclooxy, hydroxyamino, alkoxyamino
• Haloalkyl refers to alkyl as defined herein substituted by 1-4 halo groups as defined herein, which may be the same or different.
• Representative haloalkyl groups include, by way of example, trifluoromethyl, 3-fluorododecyl, 12,12,12-trifluorododecyl, 2-bromooctyl, 3-bromo-6-chloroheptyl, and the like.
• heteroaryl refers to an aromatic group of from 1 to 15 carbon atoms and 1 to 4 heteroatoms selected from oxygen, nitrogen and sulfur within at least one ring (if there is more than one ring).
• heteroaryl groups can be optionally substituted with 1 to 5 substituents, preferably 1 to 3 substituents, selected from the group consisting of acyloxy, hydroxy, thiol, acyl, alkyl, alkoxy, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, substituted alkyl, substituted alkoxy, substituted alkenyl, substituted alkynyl, substituted cycloalkyl, substituted cycloalkenyl, amino, substituted amino, aminoacyl, acylamino, alkaryl, aryl, aryloxy, azido, carboxy, carboxyalkyl, cyano, halo, nitro, heteroaryl, heteroaryloxy, heterocyclic, heterocyclooxy, aminoacyloxy, oxyacylamino, thioalkoxy, substituted thioalkoxy, thioaryloxy,
• Preferred aryl substituents include alkyl, alkoxy, halo, cyano, nitro, trihalomethyl, and thioalkoxy.
• Such heteroaryl groups can have a single ring (e.g., pyridyl or furyl) or multiple condensed rings (e.g., indolizinyl or benzothienyl).
• Preferred heteroaryls include pyridyl, pyrrolyl and furyl.
• Heteroarylalkyl refers to (heteroaryl)alkyl- where heteroaryl and alkyl are as defined herein. Representative examples include 2-pyridylmethyl and the like.
• heteroaryloxy refers to the group heteroaryl-O-.
• heteroarylene refers to the diradical group derived from heteroaryl (including substituted heteroaryl), as defined above, and is exemplified by the groups 2,6-pyridylene, 2,4-pyridiylene, 1 ,2-quinolinylene, 1,8-quinolinylene, 1 ,4-benzofuranylene, 2,5-pyridnylene, 2,5-indolenyl and the like.
• heterocycle or “heterocyclic” refers to a monoradical saturated or unsaturated group having a single ring or multiple condensed rings, from 1 to 40 carbon atoms and from 1 to 10 hetero atoms, preferably 1 to 4 heteroatoms, selected from nitrogen, sulfur, phosphorus, and/or oxygen within the ring.
• heterocyclic groups can be optionally substituted with 1 to 5, and preferably 1 to 3 substituents, selected from the group consisting of alkoxy, substituted alkoxy, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, acyl, acylamino, acyloxy, amino, substituted amino, aminoacyl, aminoacyloxy, oxyaminoacyl, azido, cyano, halogen, hydroxyl, keto, thioketo, carboxy, carboxyalkyl, thioaryloxy, thioheteroaryloxy, thioheterocyclooxy, thiol, thioalkoxy, substituted thioalkoxy, aryl, aryloxy, heteroaryl, heteroaryloxy, heterocyclic, heterocyclooxy, hydroxyamino, alkoxyamino, nitro,
• heterocyclic groups can have a single ring or multiple condensed rings.
• Preferred heterocyclics include morpholino, piperidinyl, and the like.
• nitrogen heterocycles and heteroaryls include, but are not limited to, pyrrole, imidazole, pyrazole, pyridine, pyrazine, pyrimidine, pyridazine, indolizine, isoindole, indole, indazole, purine, quinolizine, isoquinoline, quinoline, phthalazine, naphthylpyridine, quinoxaline, quinazoline, cinnoline, pteridine, carbazole, carboline, phenanthridine, acridine, phenanthroline, isothiazole, phenazine, isoxazole, phenoxazine, phenothiazine, imidazolidine, imidazoline, piperidine, piperazine
• crown compounds refers to a specific class of heterocyclic compounds having one or more repeating units of the formula [-(CH 2 -) a A-] where a is equal to or greater than 2, and A at each separate occurrence can be O, N, S or P.
• Examples of crown compounds include, by way of example only, [-(CH 2 ) 3 -NH-] 3 , [-((CH 2 ) 2 -O) 4 - ((CH 2 ) 2 -NH) 2 ] and the like.
• crown compounds can have from 4 to 10 heteroatoms and 8 to 40 carbon atoms.
• heterocyclooxy refers to the group heterocyclic-O-.
• thioheterocyclooxy refers to the group heterocyclic-S-.
• oxyacylamino or “aminocarbonyloxy” refers to the group -OC(O)NRR where each R is independently hydrogen, alkyl, substituted alkyl, aryl, heteroaryl, or heterocyclic wherein alkyl, substituted alkyl, aryl, heteroaryl and heterocyclic are as defined herein.
• prodrug is well understood in the art and includes compounds that are converted to pharmaceutically active compounds of the invention in a mammalian system. For example, see Remington 's Pharmaceutical Sciences, 1980, vol. 16, Mack Publishing Company, Easton, Pennsylvania, 61 and 424.
• saccharide group refers to an oxidized, reduced or substituted saccharide monoradical covalently attached to the glycopeptide or other compound via any atom of the saccharide moiety, preferably via the aglycone carbon atom.
• saccharide group includes amino-containing saccharide groups.
• Representative saccharides include, by way of illustration, hexoses such as D- glucose, D-mannose, D-xylose, D-galactose, vancosamine, 3-desmethyl- vancosamine, 3-epi-vancosamine, 4-epi-vancosamine, acosamine, actinosamine, daunosamine, 3-epi-daunosamine, ristosamine, D-glucamine, N-methyl-D- glucamine, D-glucuronic acid, N-acetyl-D-glucosamine, N-acetyl-D- galactosamine, sialyic acid, iduronic acid, L-fucose, and the like; pentoses such as D-ribose or D-arabinose; ketoses such as D-ribulose or D-fructose; disaccharides such as 2-O-( ⁇ -L-vancosaminyl)- ⁇ -D-glucopyranose, 2-O-
• amino-containing saccharide group refers to a saccharide group having an amino substituent.
• Representative amino- containing saccharides include L-vancosamine, 3 -desm ethyl- vancosamine, 3-epi- vancosamine, 4-epi-vancosamine, acosamine, actinosamine, daunosamine, 3-epi- daunosamine, ristosamine, N-methyl-D-glucamine and the like.
• spiro-attached cycloalkyl group refers to a cycloalkyl group attached to another ring via one carbon atom common to both rings.
• stereoisomer as it relates to a given compound is well understood in the art, and refers another compound having the same molecular formula, wherein the atoms making up the other compound differ in the way they are oriented in space, but wherein the atoms in the other compound are like the atoms in the given compound with respect to which atoms are joined to which other atoms (e.g. an enantiomer, a diastereomer, or a geometric isomer). See for example, Morrison and Boy de Organic Chemistry, 1983, 4th ed., Allyn and Bacon, Inc., Boston, Mass., page 123
• sulfonamide refers to a group of the formula -SO 2 ⁇ RR, where each R is independently hydrogen, alkyl, substituted alkyl, aryl, heteroaryl, or heterocyclic wherein alkyl, substituted alkyl, aryl, heteroaryl and heterocyclic are as defined herein.
• thiol refers to the group -SH.
• thioalkoxy refers to the group -S-alkyl.
• substituted thioalkoxy refers to the group -S-substituted alkyl.
• thioaryloxy refers to the group aryl-S- wherein the aryl group is as defined above including optionally substituted aryl groups also defined above.
• heteroaryloxy refers to the group heteroaryl-S- wherein the heteroaryl group is as defined above including optionally substituted aryl groups as also defined above.
• thioether derivatives when used to refer to the glycopeptide compounds of this invention includes thioethers (-S-), sulfoxides (-SO-) and sulfones (-SO 2 -).
• thioethers thioethers
• sulfoxides -SO-
• sulfones -SO 2 -
• any of the above groups which contain one or more substituents it is understood, of course, that such groups do not contain any substitution or substitution patterns which are sterically impractical and/or synthetically non- feasible.
• the compounds of this invention include all stereochemical isomers arising from the substitution of these compounds.
• Cyclodextrin refers to cyclic molecules containing six or more ⁇ -D- glucopyranose units linked at the 1,4 positions by ⁇ linkages as in amylose.
• cyclodextrin also includes cyclodextrin derivatives such as hydroxypropyl and sulfobutyl ether cyclodextrins. Such derivatives are described for example, in U.S. Patent Nos. 4,727,064 and 5,376,645.
• One preferred cyclodextrin is hydroxypropyl ⁇ -cyclodextrin having a degree of substitution of from about 4.1-5.1 as measured by FTIR.
• glycopeptide refers to oligopeptide (e.g. a heptapeptide) antibiotic, characterized by a multi-ring peptide core optionally substituted with saccharide groups, such as vancomycin. Examples of glycopeptides included in this definition may be found in "Glycopeptides Classification, Occurrence, and Discovery", by Raymond C. Rao and Louise W. Crandall, ("Drugs and the Pharmaceutical Sciences”Volume 63, edited by Ramakrishnan Nagarajan, published by Marcal Dekker, Inc.).
• glycopeptodes are disclosed in U.S. Patent Nos. 4,639,433; 4,643,987; 4,497,802; 4,698,327; 5,591,714; 5,840,684; and 5,843,889; in EP 0 802 199; EP 0 801 075; EP 0 667 353; WO 97/28812; WO 97/38702; WO 98/52589; WO 98/52592; and in J Amer. Chem. Soc, 1996, 118, 13107-13108; J. Amer. Chem. Soc, 1997, 119, 12041-12047; and J Amer. Chem. Soc, 1994, 116, 4573-4590.
• Representative glycopeptides include those identified as A477, A35512, A40926, A41030, A42867, A47934, A80407, A82846, A83850, A84575, AB-65, Actaplanin,
• Actinoidin Ardacin, Avoparcin, Azureomycin, Balhimycin, Chloroorientiein, Chloropolysporin, Decaplanin, N-demethyl-vancomycin, Eremomycin, Galacardin, Helvecardin, Izupeptin, Kibdelin, LL-AM374, Mannopeptin, MM45289, MM47756, MM47761, MM49721, MM47766, MM55260, MM55266, MM55270, MM56597, MM56598, OA-7653, Orenticin, Parvodicin, Ristocetin, Ristomycin, Synmonicin, Teicoplanin, UK-68597, UK-69542, UK- 72051, Vancomycin, and the like.
• glycopeptide as used herein is also intended to include the general class of peptides disclosed above on which the sugar moiety is absent, i.e. the aglycone series of glycopeptides. For example, removal of the disaccharide moiety appended to the phenol on vancomycin by mild hydrolysis gives vancomycin aglycone. Also within the scope of the invention are glycopeptides that have been further appended with additional saccharide residues, especially aminoglycosides, in a manner similar to vancosamine.
• Vancomycin refers to a glycopeptide antibiotic having the formula:
• N van - indicates that a substituent is covalently attached to the amino group of the vacosamine moiety of vacomycin.
• N leu - indicates that a substituent is covalently attached to the amino group of the leucine moiety of vancomycin.
• “Optional” or “optionally” means that the subsequently described event or circumstance may or may not occur, and that the description includes instances where said event or circumstance occurs and instances in which it does not.
• “optionally substituted” means that a group may or may not be substituted with the described substituent.
• inert organic solvent or “inert solvent” or “inert diluent” mean a solvent or diluent which is essentially inert under the conditions of the reaction in which it is employed as a solvent or diluent.
• inert solvents or diluents include, by way of illustration, benzene, toluene, acetonitrile, tetrahydrofuran (“THF”), dimethyl formamide (“DMF”), chloroform ("CHC1 3 "), methylene chloride (or dichloromethane or "CH 2 C1 2 ), di ethyl ether, ethyl acetate, acetone, methylethyl ketone, methanol, ethanol, propanol, isopropanol, tert-butanol, dioxane, pyridine, and the like.
• the solvents used in the reactions of the present invention are inert solvents.
• “Pharmaceutically acceptable salt” means those salts which retain the biological effectiveness and properties of the parent compounds and which are not biologically or otherwise harmful as the dosage administered.
• the compounds of this invention are capable of forming both acid and base salts by virtue of the presence of amino and carboxy groups respectively.
• nitrogen- linked or "N-linked” means a group or substituent is attached to the remainder of a compound (e.g. a compound of formula I) tlirough a bond to a nitrogen of the group or substituent.
• oxygen-linked means a group or substituent is attached to the remainder of a compound (e.g. a compound of formula I) through a bond to an oxygen of the group or substituent.
• sulfur-linked means a group or substituent is attached to the remaiiider of a compound (e.g. a compound of formula I) through a bond to a sulfur of the group or substituent.
• Pharmaceutically acceptable base addition salts may be prepared from inorganic and organic bases.
• Salts derived from inorganic bases include, but are not limit d to, the sodiurr., potassi m ; lithium, ammonium, calcium, and magnesium salts.
• Salts derived from organic bases include, but are not limited to, salts of primary, secondary and tertiary amines, substituted amines including naturally-occurring substituted amines, and cyclic amines, including i-Opropyiamine, trimethyl amine, di ⁇ .hylamine, triethylamine, tripropylamine. ethanolamine, 2-dimethylaminoethanol, trometharnine, lysine, arginine, his ⁇ dine.
• carboxylic acid derivatives would be useful in the practice of this invention, for example carboxylic t «cid amides, including carboxamides, lower alky! carboxamides, di(lower alkyl) carboxamides, and the like.
• Pharmaceutically acceptable acid addition salts may be prepared from morg ⁇ . ⁇ ic and organic acids.
• Salts derived from organic acids include acetic acid, pro ionic acid, glycolic acid, pyruvic acid, oxalic acid, malic acid, malonic acid, succinic acid, maleic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid, salicylic acid and the like.
• the compounds of this invention typically contain one or more chiral centers. Accordingly, this invention is intended to include racemic mixtures, diasteromers, enantiomers and mixture enriched in one or more steroisomer.
• the scope of the invention as described and claimed encompasses the racemic forms of the compounds as well as the individual enantiomers and non-racemic mixtures thereof.
• treatment includes any treatment of a condition or disease in an animal, particularly a mammal, more particularly a human, and includes:
• disease state which is alleviated by treatment with a broad spectrum antibacterial or "bacterial disease” as used herein is intended to cover all disease states which are generally acknowledged in the art to be usefully treated with a broad spectrum antibacterial in general, and those disease states which have been found to be usefully treated by the specific antibacterials of this invention.
• Such disease states include, but are not limited to, treatment of a mammal afflicted with pathogenic bacteria, in particular staphylococci (methicillin sensitive and resistant), streptococci (penicillin sensitive and resistant), enterococci (vancomycin sensitive and resistant), and Clostridium difficile
• therapeutically effective amount refers to that amount which is sufficient to effect treatment, as defined herein, when administered to a mammal in need of such treatment.
• the therapeutically effective amount will vary depending on the subject and disease state being treated, the severity of the affliction and the manner of administration, and may be determined routinely by one of ordinary skill in the art.
• protecting group refers to any group which, when bound to one or more hydroxyl, thiol, amino, carboxy or other groups of the compounds, prevents undesired reactions from occurring at these groups and which protecting group can be removed by conventional chemical or enzymatic steps to reestablish the hydroxyl, thio, amino, carboxy or other group.
• removable blocking group employed is not critical and preferred removable hydroxyl blocking groups include conventional substituents such as allyl, benzyl, acetyl, chloroacetyl, thiobenzyl, benzylidine, phenacyl, t-butyl- diphenylsilyl and any other group that can be introduced chemically onto a hydroxyl functionality and later selectively removed either by chemical or enzymatic methods in mild conditions compatible with the nature of the product.
• substituents such as allyl, benzyl, acetyl, chloroacetyl, thiobenzyl, benzylidine, phenacyl, t-butyl- diphenylsilyl and any other group that can be introduced chemically onto a hydroxyl functionality and later selectively removed either by chemical or enzymatic methods in mild conditions compatible with the nature of the product.
• Preferred removable amino blocking groups include conventional substituents such as t-butyoxycarbonyl (t-BOC), benzyloxycarbonyl (CBZ), fluorenylmethoxycarbonyl (FMOC), allyloxycarbonyl (ALOC) and the like, which can be removed by conventional conditions compatible with the nature of the product.
• t-BOC t-butyoxycarbonyl
• CBZ benzyloxycarbonyl
• FMOC fluorenylmethoxycarbonyl
• ALOC allyloxycarbonyl
• Preferred carboxy protecting groups include esters such as methyl, ethyl, propyl, t-butyl etc. which can be removed by mild conditions compatible with the nature of the product.
• glycopeptide compounds of this invention can be prepared from readily available starting materials using the following general methods and procedures. It will be appreciated that where typical or preferred process conditions (i.e., reaction temperatures, times, mole ratios of reactants, solvents, pressures, etc.) are given, other process conditions can also be used unless otherwise stated. Optimum reaction conditions may vary with the particular reactants or solvent used, but such conditions can be determined by one skilled in the art by routine optimization procedures.
• protecting groups may be necessary to prevent certain functional groups from undergoing undesired reactions.
• the choice of a suitable protecting group for a particular functional group as well as suitable conditions for protection and deprotection are well known in the art. For example, numerous protecting groups, and their introduction and removal, are described in T. W. Greene and G. M. Wuts, Protecting Groups in Organic Synthesis, Third Edition, Wiley, New York, 1999, and references cited therein.
• glycopeptide compounds are depicted in a simplified form as a box "G” that shows the carboxy terminus labeled [C], the vancosamine amino terminus labeled [V], the "non-saccharide” amino terminus (leucine amine moiety) labeled [N], and optionally, the resorcinol moiety labeled [R] as follows:
• a glycopeptide compound of the present invention that is substituted at the C-terminus with a substituent that comprises two or more carboxy groups can be prepared by coupling the corresponding glycopeptide compound wherein the C-terminus is a carboxy group with the requisite carboxy-protected compound. Subsequent removal of the carboxy protecting groups affords the compound of the invention.
• a glycopeptide compound of formula I wherein R 3 is a nitrogen linked moiety comprising two or more carboxy groups can be prepared by coupling a corresponding glycopeptide compound of formula I wherein R 3 is hydroxy with the requisite carboxy-protected amine to form an amide. Subsequent removal of the carboxy protecting groups affords the compound of the invention.
• a glycopeptide compound of the present invention wherein the C- terminus is substituted with a substituent that comprises two or more carboxy groups and wherein the vancosamine amino terminus (V) is also substituted can be prepared by first reductively alkylating the corresponding glycopeptide compound wherein the vancosamine amino terminus (V) is the free amine (NH 2 ) and then coupling the corresponding glycopeptide compound with the requisite poly carboxy-protected compound at the C-terminus. Subsequent removal of the carboxy protecting groups affords the compound of the invention.
• glycopeptide compound such as vancomycin
• a glycopeptide compound can first be reductive alkylated as shown in the following reaction:
• A represents R a minus one carbon atom and R a , R b , Y, Z and x are as defined herein.
• This reaction is typically conducted by first contacting one equivalent of the glycopeptide, i.e., vancomycin, with an excess, preferably from 1.1 to 1.3 equivalents, of the desired aldehyde in the presence of an excess, preferably about 2.0 equivalents, of a tertiary amine, such as diisopropylethylamine (DIPEA) and the like.
• DIPEA diisopropylethylamine
• This reaction is typically conducted in an inert diluent, such as DMF or acetonitrile/water, at ambient temperature for about 0.25 to 2 hours until formation of the corresponding imine and/or hemiaminal is substantially complete.
• the resulting imine and/or hemiaminal is typically not isolated, but is reacted in situ with a metal hydride reducing agent, such as sodium cyanoborohydride and the like, to afford the corresponding amine.
• a metal hydride reducing agent such as sodium cyanoborohydride and the like
• This reaction is preferably conducted by contacting the imine and/or hemiaminal with an excess, preferably about 3 equivalents, of trifluoroacetic acid, followed by about 1 to 1.2 equivalents of the reducing agent at ambient temperature in methanol or acetonitrile/water.
• the resulting alkylated product is readily purified by conventional procedures, such as precipitation and/or reverse-phase HPLC.
• conventional procedures such as precipitation and/or reverse-phase HPLC.
• the selectivity for the reductive alkylation reaction is greatly improved, i.e., reductive alkylation at the amino group of the saccharide (e.g., vancosamine) is favored over reductive alkylation at the N-terminus (e.g., the leucinyl group) by at least 10:1, more preferably 20:1.
• the present invention also provides a method for alkylatmg a glycopeptide that comprises a saccharide-amine comprising: combining an aldehyde or ketone, a suitable base, and the glycopeptide, to provide a reaction mixture; acidifying the reaction mixture; and combining the reaction mixture with a suitable reducing agent, to provide a glycopeptide that is alkylated at the saccharide-amine.
• the glycopeptide comprises at least one amino group other than the saccharide- amine.
• the reductive alkylation at the saccharide-amine is favored over reductive alkylation at another amino group of the glycopeptide by at least about 10:1; and more preferably, by at least about 15:1 or about 20: 1.
• the reductive alkylation process of the invention is typically carried out in the presence of a suitable solvent or combination of solvents, such as, for example, a halogenated hydrocarbon (e.g. methylene chloride), a linear or branched ether (e.g. diethyl ether, tetrahydrofuran), an aromatic hydrocarbon (e.g.
• a suitable solvent or combination of solvents such as, for example, a halogenated hydrocarbon (e.g. methylene chloride), a linear or branched ether (e.g. diethyl ether, tetrahydrofuran), an aromatic hydrocarbon (e.g.
• benzene, or toluene an alcohol (methanol, ethanol, or isopropanol), dimethylsulfoxide (DMSO), N,N-dimethylformamide, acetonitrile, water, 1,3- dimethyl-3,4,5,6-tetrahydro-2(lH)-pyrimidone, tetramethyl urea, N,N- dimethylacetamide, diethylformamide (DMF), l-methyl-2-pyrrolidinone, tetramethylenesulfoxide, glycerol, ethyl acetate, isopropyl acetate, N,N- dimethylpropylene urea (DMPU) or dioxane.
• DMSO dimethylsulfoxide
• DMF diethylformamide
• DMF diethylformamide
• l-methyl-2-pyrrolidinone tetramethylenesulfoxide
• glycerol ethyl acetate
• the alkylation is carried out in acetonitrile/water, or DMF/methanol.
• the reduction i.e. treatment with the reducing agent
• a protic solvent such as, for example, an alcohol (e.g. methanol, ethanol, propanol, isopropanol, or butanol), water, or the like.
• the reductive alkylation process of the invention can be carried out at any suitable temperature from the freezing point to the reflux temperature of the reaction mixture.
• the reaction is carried out at a temperature in the range of about 0 °C to about 100 °C. More preferably at a temperature in a range of about 0 °C to about 50 °C, or in a range of about 20 °C to about 30 °C.
• Suitable bases include tertiary amines (e.g. diisopropylethylamine, N-methylmorpholine or triethylamine) and the like.
• Any suitable acid can be used to acidify the reaction mixture.
• Suitable acids include carboxylic acids (e.g. acetic acid, trichloroacetic acid, citric acid, fonnic acid, or trifluoroacetic acid), mineral acids (e.g. hydrochloric acid, sulfuric acid, or phosphoric acid), and the like.
• a preferred acid is trifluoroacetic acid.
• Suitable reducing agents for carrying out reductive alkylation process of the invention are known in the art. Any suitable reducing agent can be employed in the methods of the invention, provided it is compatible with the functionality present in the glycopeptide.
• suitable reducing agents include sodium cyanoborohydri.de, triacetoxyborohydride, pyridine/borane, sodium borohydride, and zinc borohydride.
• the reduction can also be carried out in the presence of a transition metal catalyst (e.g. palladium or platinum) in the presence of a hydrogen source (e.g. hydrogen gas or cycloheadiene). See for example, , Advanced Organic Chemistry, Fourth Edition, John Wiley & Sons, New York (1992), 899-900.
• glycopeptide derivative resulting from the reductive alkylation can then coupled with a polycarboxy-substituted amine (R 3 -H) to form an amide.
• R 3 -H polycarboxy-substituted amine
• R 3 is a nitrogen-linked group that comprises at least two protected carboxy groups.
• the glycopeptide derivative is typically contacted with the amine in the presence of a peptide coupling reagent, such as PyBOP and HOBT, to provide the amide.
• a peptide coupling reagent such as PyBOP and HOBT
• This reaction is typically conducted in an inert diluent, such as DMF, at a temperature ranging from about 0°C to about 60 °C for about 1 to 24 hours or until the coupling reaction is substantially complete. Subsequent deprotection using conventional procedures and reagents affords the compound of this invention.
• the amine coupling step described above can be conducted first to provide an amide, followed by reductive alkylation and deprotection to afford the compound of the invenxion.
• the glycopeptide compounds of this invention can also be prepared in a step-wise manner in which a precursor to the -R a -Y-R b -(Z) X group is first attached the glycopeptide by reductive alkylation, followed by subsequent. elaboration of the attached precursor using conventional reagent and procedures to form the -R a -Y-R -(Z) X group. Additionally, ketones may also be employed in the above-described reductive alkylation reactions to afford ⁇ -substituted amines.
• glycopeptide having an amino group may be employed in these reductive alkylation reactions.
• Such glycopeptides are well-known in the art and are either commercially available or may be isolated using conventional procedures. Suitable glycopeptides are disclosed, by way of example, in U.S. Patent Nos.
• an aminoalkyl sidechain at the resorcinol moiety of a glycopeptide such as vancomycin
• a Mannich reaction in this scheme, the resorcmol moiety is shown for clarity.
• an amine NHR C R C
• an aldehyde CH 2 O
• formalin a source of formaldehyde
• Suitable reagents for oxidizing a thio compound to a sulfoxide include, by way of example, hydrogen peroxide, peracides such as 3- chloroperoxybenzoic acid (MCPBA), sodium periodate, sodium chlorite, sodium hypochlorite, calcium hypochlorite, tert-butyl hypochlorite and the like.
• Chiral oxidizing reagents, (optically active reagents) may also be employed to provide chiral sulfoxides.
• optically active reagents are well-known in the art and include, for example, the reagents described in Kagen et al., Synlett., 1990, 643- 650.
• the aldehydes and ketones employed in the above reactive alkylation reactions are also well-known in the art and are either commercially available or can be prepared by conventional procedures using commercially available starting materials and conventional reagents (for example see March, Advanced Organic Chemistry, Fourth Edition, John Wiley & Sons, New York (1992), and references cited therein).
• poly-carboxy-substituted compounds e.g. poly-carboxy-substituted amines, alcohols, and thiols
• employed to prepare the compounds of the invention are either commercially available or can be prepared by conventional procedures using commercially available starting materials and reagents.
• This invention also includes pharmaceutical composition containing the novel glycopeptide compounds of this invention.
• the glycopeptide compound preferably in the form of a pharmaceutically acceptable salt, can be formulated for oral or parenteral administration for the therapeutic or prophylactic treatment of bacterial infections.
• the glycopeptide compound can be admixed with conventional pharmaceutical carriers and excipients and used in the form of tablets, capsules, elixirs, suspensions, syrups, wafers, and the like.
• Such pharmaceutical compositions will contain from about 0.1 to about 90% by weight of the active compound, and more generally from about 10 to about 30%.
• the pharmaceutical compositions may contain common carriers and excipients, such as corn starch or gelatin, lactose, sucrose, microcrystall ne cellulose, kaolin, mannitol, dicalcium phosphate, sodium chloride, and alginic acid.
• Disintegrators commonly used in the formulations of this invention include croscarmellose, microcrystalline cellulose, corn starch, sodium starch glycolate and alginic acid.
• a liquid composition will generally consist of a suspension or solution of the compound or pharmaceutically acceptable salt in a suitable liquid carrier(s), for example ethanol, glycerine, sorbitol, non-aqueous solvent such as polyethylene glycol, oils or water, optionally with a suspending agent, a solubilizi g agent (such as a cyclodextrin), preservative, surfactant, wetting agent, flavoring or coloring agent.
• a liquid formulation can be prepared from a reconstitutable powder.
• a powder containing active compound, suspending agent, sucrose and a sweetener can be reconstituted with water to form a suspension; and a syrup can be prepared from a powder containing active ingredient, sucrose and a sweetener.
• a composition in the form of a tablet can be prepared using any suitable pharmaceutical carrier(s) routinely used for preparing solid compositions. Examples of such carriers include magnesium stearate, starch, lactose, sucrose, microcrystalline cellulose and binders, for example polyvinylpyrrolidone.
• the tablet can also be provided with a color film coating, or color included as part of the carrie ⁇ (s).
• active compound can be formulaied in a controlled release dosage form as a tablet comprising a hydrophilic or hydrophobic matrix.
• a composition in the form of a capsule can be prepared using routine encapsulation procedures, for example by incorporation of active compound and excipients into a hard gelatin capsule.
• a semi-solid matrix of active compound and high molecular weight polyethylene glycol can be prepared and filled into a hard gelatin capsule; or a solution of active compound in polyethylene glycol or a suspension in edible oil, for example liquid paraffin or fractionated coconut oil can be prepared and filled into a soft gelatin capsule.
• Tablet binders that can be included are acacia, methylcellulose, sodium carboxymefhylcellulose, poly-vinylpyrrolidone (Povidone), hydroxypropyl methylcellulose, sucrose, starch and ethylcellulose.
• Lubricants that can be used include magnesium stearate or other metallic stearates, stearic acid, silicone fluid, talc, waxes, oils and colloidal silica. Flavoring agents such as peppermint, oil of wintergreen, cherry flavoring or the like can also be used. Additionally, it may be desirable to add a coloring agent to make the dosage form more attractive in appearance or to help identify the product.
• the compounds of the invention and their pharmaceutically acceptable salts that are active when given parenterally can be formulated for intramuscular, intrathecal, or intravenous administration.
• a typical composition for intra-muscular or intrathecal administration will consist of a suspension or solution of active ingredient in an oil, for example arachis oil or sesame oil.
• a typical composition for intravenous or intrathecal administration will consist of a sterile isotonic aqueous solution containing, for example active ingredient and dextrose or sodium chloride, or a mixture of dextrose and sodium chloride.
• Other examples are lactated Ringer's injection, lactated Ringer's plus dextrose injection, Normosol-M and dextrose, Isolyte E, acylated Ringer's injection, and the like.
• a co-solvent for example, polyethylene glycol
• a chelating agent for example, ethylenediamine tetracetic acid
• a solubilizing agent for example, a cyclodextrin
• an anti-oxidant for example, sodium metabisulphite
• the solution can be freeze dried and then reconstituted with a suitable solvent just prior to administration.
• the glycopeptide derivatives of this invention are formulated in an aqueous solution containing a cyclodextrin.
• the glycopeptide derivatives of this invention are formulated as a lyophilized powder containing a cyclodextrin or as a sterile powder containing a cyclodextrin.
• the cyclodextrin is hydroxypropyl- ⁇ -cyclodextrin or sulfobutyl ether ⁇ -cyclodextrin; more preferably, the cyclodextrin is hydroxypropyl- ⁇ -cyclodextrin.
• the cyclodextrin will comprise about 1 to 25 weight percent; preferably, about 2 to 10 weight percent; more preferable, about 4 to 6 weight percent, of the formulation. Additionally, the weight ratio of the cyclodextrin to the glycopeptide derivative will preferably be from about 1:1 to about 10:1.
• the compounds of the invention and their pharmaceutically acceptable salts which are active on rectal administration can be formulated as suppositories.
• a typical suppository formulation will generally consist of active ingredient with a binding and/or lubricating agent such as a gelatin or cocoa butter or other low melting vegetable or synthetic wax or fat.
• transdermal compositions or transdermal delivery devices
• Such compositions include, for example, a backing, active compound reservoir, a control membrane, liner and contact adhesive.
• transdermal patches may be used to provide continuous or discontinuous infusion of the compounds of the present invention in controlled amounts.
• the construction and use of transdermal patches for the delivery of pharmaceutical agents is well known in the art. See, e.g., U.S. Patent 5,023,252, issued June 11, 1991.
• patches may be constructed for continuous, pulsatile, or on demand delivery of pharmaceutical agents.
• the active compound is effective over a wide dosage range and is generally administered in a pharmaceutically effective amount. It, will be understood, however, that the amount of the compound actually administered will be determined by a physician, in the light of the relevant circumstances, including the condition to be treated, the chosen route of administration, the actual compound administered and its relative activity, the age, weight, and response of the individual patient, the severity of the patient's symptoms, and the like.
• Suitable doses are in the general range of from 0.01-100 mg/kg/day, preferably 0.1-50 mg/kg/day. For an average 70 kg human, this would amount to 0.7 mg to 7 g per day, or preferably 7 mg to 3.5 g per day. A more preferred dose for a human is about 500 mg to about 2 g per day.
• Formulation Example A This example illustrates the preparation of a representative pharmaceutical composition for oral administration of a compound of this invention: Ingredients Quantity per tablet, (mg)
• Active Compound 200 Lactose, spray-dried 148 Magnesium stearate 2
• the above ingredients are mixed and introduced into a hard-shell gelatin capsule.
• Formulation Example B This example illustrates the preparation of another representative pharmaceutical composition for oral administration of a compound of this invention:
• This example illustrates the preparation of a representative pharmaceutical composition for oral administration of a compound of this invention.
• An oral suspension is prepared having the following composition. Ingredients
• Veegum K (Vanderbilt Co.) 1.0 g Flavoring 0.035 ml
• Formulation Example D This example illustrates the preparation of a representative pharmaceutical composition containing a compound of this invention.
• An injectable preparation buffered to a pH of 4 is prepared having the following composition:
• Formulation Example E This example illustrates the preparation of a representative pharmaceutical composition for injection of a compound of this invention.
• a reconstituted solution is prepared by adding 20 mL of sterile water to 1 g of the compound of this invention. Before use, the solution is then diluted with 200 mL of an intravenous fluid that is compatible with the active compound.
• Such fluids are chosen from 5% dextrose solution, 0.9% sodium chloride, or a mixture of 5% dextrose and 0.9% sodium chloride.
• Other examples are lactated Ringer's injection, lactated Ringer's plus 5% dextrose injection, Normosol-M and 5% dextrose, Isolyte E, and acylated Ringer's injection Formulation Example F This example illustrates the preparation of a representative pharmaceutical composition containing a compound of this invention.
• An injectable preparation is prepared having the following composition:
• Active Compound 0.1-5.0 g Hydroxypropyl- ⁇ -cyclodextrin 1-25 g 5% Aqueous Dextrose Solution (sterile) q.s. to 100 mL
• Formulation Example G This example illustrates the preparation of a representative pharmaceutical composition containing a compound of this invention.
• a frozen solution suitable for injection is prepared having the following composition:
• the weight ratio of hydroxypropyl- ⁇ -cyclodextrin to the active compound will typically be from about 1:1 to about 10:1.
• Formulation Example H This example illustrates the preparation of a representative pharmaceutical composition containing a compound of this invention.
• a lyophilized powder useful for preparing an injectable solution is prepared having the following composition:
• Excipients e.g., mannitol, sucrose and/or lactose 0-50 g
• Buffer agent e.g., citrate 0-500 mg
• the weight ratio of hydroxypropyl- ⁇ -cyclodextrin to the active compound will typically be from about 1:1 to about 10:1.
• Hydroxypropyl- ⁇ -cyclodextrin and excipients and/or buffering agents are dissolved in about 60% of the water for injection.
• the active compound is added and dissolved and the pH is adjusted with 1 M sodium hydroxide to 4.0-5.0 and the volume is adjusted to 95% of the final volume with water for injection.
• the pH is checked and adjusted, if necessary, and the volume is adjusted to the final volume with water for injection.
• the formulation is then sterile filtered through a 0.22 micron filter and placed into a sterile vial under aseptic conditions.
• the formulation is then freeze-dried using an appropriate lyophihzation cycle.
• the vial is capped (optionally under partial vacuum or dry nitrogen), labeled and stored at room temperature or under refrigeration.
• Formulation Example I This example illustrates the preparation of a representative pharmaceutical composition containing a compound of this invention.
• a sterile powder useful for preparing an injectable solution is prepared having the following composition:
• the weight ratio of hydroxypropyl- ⁇ -cyclodextrin to the active will typically be from about 1:1 to about 10:1.
• Hydroxypropyl- ⁇ -cyclodextrin and the active compound (and any excipients) are dispersed into an appropriate sterile container and the container is sealed (optionally under partial vacuum or dry nitrogen), labeled and stored at room temperature or under refrigeration.
• formulation examples H and I above can be administered intravenously to a patient by the appropriate medical personnel to treat or prevent gram-positive infections.
• the above formulations can be reconstituted and/or diluted with a diluent, such as 5% dextrose or sterile saline, as follows:
• This example illustrates the preparation of a representative pharmaceutical composition containing a compound of this invention.
• a suppository totaling 2.5 grams is prepared having the following composition:
• Witepsol H-15 * balance ( * triglycerides of saturated vegetable fatty acid; a product of Riches-
• glycopeptide compounds of this invention are useful in medical treatments and exhibit biological activity, including antibacterial activity, which can be demonstrated in using the tests described herein. Such tests are well known to those skilled in the art, and are referenced and described in Lorian “Antibiotics in Laboratory Medicine", Fourth Edition, Williams and Wilkins (1991).
• this invention provides methods for treating bacterial or infectious diseases, especially those caused by Gram-positive microorganisms, in animals.
• the compounds of this invention are particularly useful in treating infections caused by methicillin-resistant staphylococci.
• the compounds are useful in treating infection due to enterococci, including vancomycin- resistant enterococci (VRE).
• VRE vancomycin- resistant enterococci
• diseases include severe staphylococcal infections, such as staphylococcal endocarditis and staphylococcal septicemia.
• the animal treated may be either susceptible to, or infected with, the microorganism.
• the method of treatment typically comprises administering to the animal an amount of a compound of this invention which is effective for this purpose.
• the antibiotic can be administered in a single daily dose or in multiple doses per day.
• the treatment regimen may require administration over extended periods of time, for example, for several days or for from one to six weeks.
• the amount per administered dose or the total amount administered will depend on such factors as the nature and severity of the infection, the age and general health of the patient, the tolerance of the patient to the antibiotic and the microorganism or microorganisms in the infection.
• vancomycin hydrochloride semi-hydrate was purchased from Alpharma, Inc. Fort Lee, ⁇ J 07024 (Alpharma AS, Oslo Norway).
• Other reagents and reactants are available from Aldrich Chemical Co.,
• R 18 is hydrogen
• R 19 is hydrogen
• R 20 is -CH 2 CH 2 -S-(CH 2 ) 9 CH 3 )
• R 18 is hydrogen
• R 19 is hydrogen
• R 20 is -CH 2 CH 2 O(CH 2 ) 9 CH 3 N VAN -decyloxoethyl Vancomycin bistrifluoroacetate (3 g, 1.6 mmol) and
• MTCs Minimal Inhibitory Concentrations
• Vancomycin resistant enterococci were phenotyped as Van A or Van B based on their sensitivity to teicoplanin. Some vancomycin resistant enterococci that had been genotyped as Van A, Van B, Van Cl or Van C2 were obtained from the Mayo Clinic.
• MICs Minimal inhibitory concentrations were measured in a microdilution broth procedure under NCCLS guidelines. Routinely, the compounds were serially diluted into Mueller-Hinton broth in 96-well microtiter plates. Overnight cultures of bacterial strains were diluted based on absorbance at 600 ran so that the final concentration in each well was 5 x 10 5 cfu/rnL. Plates were returned to a 35 °C incubator. The following day (or 24 hours in the case of Enterococci strains), MICs were determined by visual inspection of the plates.
• MSSA methicillin-sensitive Staphylococcus aureus
• MSSE methicillin-sensitive Staphylococcus epidermidis
• MRSE methicillin-resistant Staphylococcus epidermidis
• VSE Fm vancomycin sensitive Enterococcus faecalis
• VRE Fm Van A vancomycin resistant Enterococcus faecium sensitive to teicoplanin
• VRE Fm Van B vancomycin resistant Enterococcus faecalis also resistant to teicoplanin
• VRE Fs Van A vancomycin resistant Enterococcus faecalis sensitive to teicoplanin
• VRE Fs Van B vancomycin resistant Enterococcus faecalis also resistant to teicoplanin
• VRE Fs Van A vancomycin resistant Enterococcus faecalis sensitive to teicoplanin
• VRE Fs Van B enterococcus
• MICs with those strains were determined using either TS A broth supplemented with defibrinated blood or blood agar plates. Compounds which had significant activity against the strains mentioned above were then tested for MIC values in a larger panel of clinical isolates including the species listed above as well as non- speciated coagulase negative Staphylococcus both sensitive and resistant to methicillin (MS-CNS and MR-CNS). In addition, they were tested for MICs against gram negative organisms, such as Escherichia coli and Pseudomonas aeruginosa.
• the compounds of the invention were active in the above tests in vitro tests and demonstrated a broad spectrum of activity.
• a compound of this invention was administered either intravenously or subcutaneously and observed for 5-15 minutes. If there were no adverse effects, the dose was increased in a second group of mice. This dose incrementation continued until mortality occurred, or the dose was maximized. Generally, dosing began at 20 mg/kg and increased by 20 mg/kg each time until the maximum tolerated dose (MTD) is achieved.
• MTD maximum tolerated dose
• mice were initially rendered neutropenic by administration of cyclophosphamide at 200 mg/kg on day 0 and day 2. On day 4 they were infected in the left anterior thigh by an IM injection of a single dose of bacteria. The mice were then administered the test compound one hour after the bacteria and at various later times (normally 1, 2.5, 4 and 24 hours) the mice were sacrificed (3 per time point) and the thigh excised, homogenized and the number of CFUs (colony forming units) were determined by plating. Blood was also plated to determine the CFUs in the blood.
• an appropriately virulent strain of bacteria most commonly S. aureus, or E. Faecalis or E. Faecium, sensitive or resistant to vancomycin.
• Mice were initially rendered neutropenic by administration of cyclophosphamide at 200 mg/kg on day 0 and day 2. On day 4 they were infected in the left anterior thigh by an IM injection of a single dose of bacteria. The mice were then administered the test compound
• the rate at which a compound of this invention is removed from the blood can be determined in either rats or mice.
• rats the test animals were cannulated in the jugular vein.
• the test compound was administered via tail vein injection, and at various time points (normally 5, 15, 30,60 minutes and 2,4,6 and 24 hours) blood was withdrawn from the cannula
• mice the test compound was also administered via tail vein injection, and at various time points. Blood was normally obtained by cardiac puncture.
• the concentration of the remaining test compound was determined by HPLC.
• the compounds of the invention were active in the above test in vivo and demonstrated a broad spectrum of activity.
• This procedure is used to examine the tissue distribution, excretion and metabolism of a radiolabeled test compound in both male and female rats following intravenous infusion at 10 mg/kg.
• the test compound is formulated in 5% hydroxypropyl- ⁇ -cyclodextrin as 2.5 mg/mL solution.
• Urine and feces are cage collected over 24 hours period. At 24 hours after dosing, animals are sacrificed and tissues are removed.
• This procedure is used to evaluate tissue distribution of a test compound in rats following single dose administration by infusion.
• Two formulations are used: 30% PEG 400 and 10% sulfobutylether- ⁇ -cyclodextrin.
• Urine samples are cage collected over 24 hours. Blood samples are collected for serum chemistry and concentration determination. Liver and kidneys are removed for histology evaluation. One kidney and part of the liver are homogenized for concentration analysis using reverse phase HPLC with UV detection. Drug concentrations in urine and serum samples are determined by LC-MS analysis.
• test compound is formulated in 5% hydroxypropyl- ⁇ -cyclodextrin or 1% sucrose/4.5% dextrose.
• Urine samples are cage collected at days 1 and 7 post-dose. Blood samples are collected for serum chemistry and concentration determination. Liver and kidneys are removed for histology evaluation. One kidney and part of the liver are homogenized for concentration analysis using reverse phase HPLC with UV detection. Drug concentrations in urine and serum samples are determined by LC- MS analysis.

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